14results about How to "Good tissue adhesion" patented technology

The invention relates to the technical field of biology, and discloses a stem cell exosome anti-hair loss and hair growth complex and a preparation method thereof.A hair growth agent is wrapped and loaded in adipose-derived mesenchymal stem cell exosome to form a drug-loaded stem cell exosome; the medicine-carrying stem cell exosome is loaded in cross-linked hydrogel composed of a gentisic acid-cyclodextrin complex and epsilon-polylysine modified hyaluronic acid to form the stem cell exosome anti-hair loss and hair growth complex, and the stem cell exosome anti-hair loss and hair growth complex is directly smeared and externally applied to the scalp surface and acts on the hair follicle infection part in a targeted mode, so that the hair loss prevention and hair growth promotion effect is achieved. The stem cell exosome anti-hair-loss and hair-growth-promoting compound can inhibit bacterial growth, relieve and treat hair follicle inflammation caused by bacterial infection and improve the scalp environment, so that under the condition that inflammation is effectively controlled, the drug-loaded stem cell exosome in the stem cell exosome anti-hair-loss and hair-growth-promoting compound is stably released, damaged hair follicle cells are repaired, hair regeneration is promoted, the exosome treatment effect is prolonged, and the anti-hair-loss and hair-growth-promoting effect is achieved. The purpose of treating alopecia is achieved.

The invention discloses a preparation method and application of photo-thermal antibacterial hydrogel for MRSA (Methicillin Resistant Staphylococcus Aureus) infected diabetes wounds, and relates to the technical field of biological tissue engineering. The method comprises the following steps: uniformly dispersing ZIF-8 (at) PDA nanoparticles in a carboxymethyl chitosan solution, adding a gelatin solution, uniformly mixing, adding into an oxidized sodium alginate solution, and carrying out a Schiff base reaction to obtain the photo-thermal antibacterial hydrogel for MRSA infected diabetes wounds. The hydrogel disclosed by the invention is prepared by crosslinking carboxymethyl chitosan, gelatin, oxidized sodium alginate and dopamine modified ZIF-8 nanoparticles through a Schiff base bond. Carboxymethyl chitosan (CMCS) has excellent biocompatibility, antibacterial activity and adjustable and controllable mechanical properties, and can effectively preserve moisture, form a breathable and moist environment and promote skin healing.

The invention provides antibacterial carboxymethyl cellulose hemostatic gauze and a preparation method thereof.The preparation method comprises the steps that carboxymethyl cellulose gauze is soaked in a PBS buffer solution, N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide are added into the PBS buffer solution, and a soaking solution is obtained after uniform mixing; adding the graft modified alginate oligosaccharide into the soaking solution, mixing and stirring to generate grafting reaction, taking out the carboxymethyl cellulose gauze after the reaction is finished, washing and drying to obtain the antibacterial carboxymethyl cellulose hemostatic gauze. According to the preparation method disclosed by the invention, the catechol-grafted modified alginate oligosaccharide and partially carboxymethylated carboxymethyl cellulose gauze are subjected to amidation grafting compounding, so that the hemostatic gauze with excellent antibacterial and hemostatic properties is prepared.

The invention provides a seawater soaking wound hydrogel dressing and a preparation method thereof, a drug-loaded nano system 4OI (at) ZIF-8 (at) PAD with a core-shell structure is constructed, an anti-inflammatory drug 4-octyl itaconic acid and zinc ions are jointly encapsulated in a ZIF-8 framework, and a polydopamine coating is wrapped, so that synergistic loading and controllable release of the drug and metal ions are realized. According to the dressing, dopamine modified hyaluronic acid is used as a matrix, a temperature-sensitive material and an enzyme catalysis cross-linking system are combined, stable gel can be rapidly formed in situ within 5 seconds, and the dressing has excellent tissue adhesion performance (the adhesion strength reaches 6.7 kPa). Experiments prove that the hydrogel dressing has the characteristics of broad-spectrum antibiosis, inflammation regulation and control and long-acting release. Animal experiments prove that the healing rate of seawater soaking wounds treated by the dressing on the twelfth day is as high as 95.7% and is remarkably superior to that of a commercial silver ion gel control group, scars are less after healing, and an efficient, safe and easy-to-implement scheme is provided for marine environment wound nursing.

The invention discloses preparation and application of a space-time cascade wound dressing, relates to the technical field of biomedical materials, and solves the problems of limited depth and poor treatment effect when a diabetes infected wound is treated in the prior art. The space-time cascade wound dressing is composed of a hydrogel patch and a soluble microneedle array. The hydrogel patch comprises polyvinyl alcohol, sodium alginate, nano-enzyme, a color reagent for indicating pH and the like, has the functions of oxidation resistance and sterilization, and can monitor the state of the wound surface in real time according to the pH change indication of the wound surface; the soluble microneedle array comprises a soluble polymer and glucose oxidase, and has the functions of deep sterilization and local blood glucose reduction. According to the space-time cascade wound dressing provided by the invention, two treatment forms of the hydrogel patch and the soluble microneedle array are integrated, and a nano enzyme and a natural enzyme catalytic system capable of generating cascade catalytic reaction are respectively introduced into the hydrogel patch and the soluble microneedle array, so that a multifunctional treatment system with a space-time cascade catalytic effect is formed; and comprehensive treatment and real-time monitoring of multi-layer wound surfaces are realized.

The application belongs to the technical field of biological medicine, and specifically discloses a sprayable double-network hydrogel microsphere adhesive as well as a preparation method and application thereof. The preparation method comprises the following steps: liquid paraffin and sorbitan monooleate, polyoxyethylene sorbitan monolaurate are taken as oil phases to form an emulsion; the emulsion is fully mixed with petroleum ether, centrifugal separation is performed on the gel hydrogel microspheres, and freeze-drying is performed on the gel hydrogel microspheres for storage in the dark; preparation of ropivacaine liposomes; ultrapure water is added into the lipid membrane to obtain a primary liposome suspension, the hydrogel microspheres are used to adsorb the liposomes, and the obtained double-network hydrogel microspheres are collected. The sprayable double-network hydrogel microsphere adhesive as well as the preparation method and application thereof can be used to prepare hydrogel microspheres which can quickly and uniformly cover irregular wounds, and the liposomes carrying ropivacaine can provide sustained analgesic release, so that the problems of maintaining sustained adhesion in a blood-saturated wound and prolonging pain relief are simultaneously solved.

The invention relates to the technical field of medical biological materials, and discloses a mussel mucin-chondroitin sulfate composite hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: respectively preparing a mussel mucin aqueous solution and a chondroitin sulfate aqueous solution; the mass volume concentration of the mussel mucoprotein aqueous solution is 1%-10%, the mass volume concentration of the chondroitin sulfate aqueous solution is 1%-10%, the mass ratio of the mussel mucoprotein to the chondroitin sulfate is 3: 1-1: 3, the two solutions are mixed and stirred or oscillated under the conditions that the temperature is 4-37 DEG C and the pH value is 7.0-9.0, a cross-linking reaction is conducted, and hydrogel is formed; the cross-linking mode is physical cross-linking, chemical cross-linking or physical-chemical dual cross-linking; according to the invention, excellent tissue adhesion, injectability, self-healing property and biodegradability are realized, cell adhesion and proliferation can be effectively promoted, and the gel is suitable for various medical beauty scenes such as skin filling, wound repairing and anti-aging nursing.

The invention belongs to the technical field of anti-freezing hydrogel, and particularly relates to anti-freezing hydrogel and application of the anti-freezing hydrogel in preparation of a rapid trauma hemostasis product, and the anti-freezing hydrogel is prepared through the following steps: 1) preparing a nanocellulose solution; 2) adding a catalyst, a cross-linking agent and dopamine into the nanocellulose solution, and reacting to obtain a first mixed solution; 3) cooling the first mixed solution, dialyzing and drying to obtain an intermediate solid product; 4) mixing the intermediate solid product with water to obtain a second mixed solution, then adding glycerol and polyvinyl alcohol, and after the intermediate solid product is completely dissolved, adding a borax solution to obtain a third mixed solution; and 5) adding a coagulation promoting drug into the third mixed solution to obtain a fourth mixed solution, reacting at 90-92 DEG C for 0.5-1 hour, cooling to room temperature, and molding to obtain the anti-freezing hydrogel. The material has excellent tissue adhesion, freezing resistance and rapid hemostatic effect.

The utility model relates to an oral cavity adhesive bandage with a double-layer structure. The oral cavity adhesive bandage comprises a near-wound layer and a protective layer, wherein the near-wound layer is directly attached to a wound surface; the protective layer is adhered to the outer side of the near-wound layer; the area of the protective layer is larger than or equal to that of the near-wound layer, so that the near-wound layer is located in the covering surface of the protective layer; the near-wound layer and the protective layer are hydrophilic structure layers; the near-wound layer is an absorptive application pad, and the protective layer is an anti-adhesion layer. The near-wound layer can be completely attached to the surface of a wound, the outer layer structure also has certain tissue adhesion, the tissue adhesion performance of the adhesive bandage is greatly improved, and the problem that an existing product is prone to slipping off is solved; meanwhile, the hydrophilic structure of the protective layer on the outer layer of the adhesive bandage can be dissolved, so that the safety problem after an existing product is mistakenly swallowed can be solved; by adding a specific high polymer material, the dissolution time of the product is remarkably prolonged, tissue healing can be promoted, breeding of oral pathogenic bacteria can be inhibited, and good safety is achieved.

The invention belongs to the technical field of high polymer materials, and particularly relates to three-layer gradient self-healing hydrogel as well as a preparation method and application thereof. The invention provides a composition for preparing three-layer gradient self-healing hydrogel. The composition comprises a composition A, a composition B and / or a composition C, the composition A and the composition B are both composed of modified chondroitin sulfate, carboxymethyl chitosan and tannic acid, and the difference is that the concentration of tannic acid in the composition A is 0.5-1.5% w / v, and the concentration of tannic acid in the composition B is 2.5-3.5% w / v; the composition C is prepared from modified chondroitin sulfate, carboxymethyl chitosan, tannic acid and nano-hydroxyapatite, wherein the concentration of the tannic acid is 2.5 to 3.5 percent w / v. The three-layer gradient self-healing hydrogel has good self-healing performance, mechanical performance, tissue adhesion performance and oxidation resistance, and can be applied to integrated repair of articular cartilage-bone tissue.

This invention relates to a myocardial repair patch based on multi-material printing, its preparation, and its application, belonging to the field of bio-3D printing and regenerative medicine technology. The invention uses PLCL and GelMA hydrogel loaded with active cardiomyocytes as bio-inks, and introduces gelatin gel as a heat buffer material. An orthogonal network structure is constructed using multi-material bio-3D printing technology to prepare a myocardial repair patch with excellent dynamic adaptability to the myocardial interface and good biocompatibility. The myocardial injury repair patch prepared by this invention achieves a structure-function integrated design through the synergistic effect of the three materials, significantly overcoming the limitations of single materials in terms of mechanical properties, biological function, or thermal compatibility. The mechanical properties of the myocardial injury repair patch prepared by this invention can precisely match those of natural myocardium, while possessing high cell activity, effectively inhibiting fibrosis, improving myocardial function, and achieving true active repair. Furthermore, the process conditions are controllable, demonstrating good translational potential.

The invention discloses a medical flushing glue solution and a preparation method thereof, and relates to the technical field of flushing glue solutions, and the medical flushing glue solution is prepared by adding 0.15% by weight of sodium methyl cellulose, 0.8% by weight of sodium chloride and 99.05% by weight of medical purified water. According to the application, the content ratio of sodium carboxymethyl cellulose to sodium chloride is accurately matched with the requirement of a medical flushing scene, and meanwhile, the dosage of sodium carboxymethyl cellulose can enable the glue solution to have good flowability and moderate tissue adhesiveness, so that smooth flushing in clinical operation can be realized, a continuous moist protective layer can be formed on the surface of tissue, and the medical flushing effect is improved. The cleaning and protecting effects are effectively achieved; the dosage of sodium chloride can accurately simulate physiological osmotic pressure to ensure that the glue solution is compatible with the human tissue environment, in addition, the glue solution under the formula is excellent in stability, has no layering and precipitation phenomena within 6 months under the storage condition of 4 DEG C, and can meet the stability requirements of long-term storage and clinical use.

The present application relates to a kind of injectable compound for soft tissue repair and its preparation method and application, the injectable compound includes: temperature-sensitive medium;Pre-crosslinked, water-insoluble hydrophilic polymer gel microparticles dispersed in the temperature-sensitive medium, content is 5-23% of the total mass of injectable compound;And acellular matrix microparticle dispersed in the temperature-sensitive medium, content is 6-12% of the total mass of injectable compound;Wherein, the network phase formed by the hydrophilic polymer gel microparticle gelatinization with the temperature-sensitive medium at body temperature is combined, constitutes the support framework of enhancing the structural stability of the injectable compound.The injectable compound involved in the present application is flowable viscous gel when injection, rapidly converted into non-flowing semi-solid at physiological temperature, forms tissue-conforming repair layer.Among them, gelatinous particle, temperature-sensitive medium, acellular matrix microparticle three synergistically promote tissue repair.

The invention relates to multifunctional in-situ repair hydrogel based on a modified dermal matrix as well as a preparation method and application of the hydrogel. The hydrogel is formed by crosslinking a first polymer precursor and a second polymer precursor through dynamic covalent bonds of boric acid ester, the first polymer precursor is carboxymethyl chitosan grafted with a phenylboronic acid group and an epsilon-polylysine chain segment; the second polymer precursor is a soluble acellular dermal matrix which is grafted with a 3, 4-dihydroxyphenyl propionic acid group and is subjected to enzymolysis treatment. The hydrogel disclosed by the invention has good biocompatibility, moldability and potential self-healing capability.