Preparation method of an infrared-responsive high-strength hydrogel for articular cartilage repair

An infrared response, cartilage repair technology, applied in medical science, prosthesis, etc., can solve the problems of gold nanomaterial toxicity, poor biocompatibility, unsuitable for cartilage repair, etc., and achieve good affinity and excellent biophase Compatibility, the effect of excellent biocompatibility

Inactive Publication Date: 2018-05-18
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are two major defects in the existing photoresponsive hydrogels: 1. The poor biocompatibility is not suitable for cartilage repair
For example, gold nanomaterials are toxic and cannot be implanted in the body; carbon nanotubes, graphene and their derivatives are inorganic substances of carbon, and long-term implantation has potential toxicity hidden dangers
2. Poor mechanical strength, not suitable for cartilage repair requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of an infrared-responsive high-strength hydrogel for cartilage repair, the specific steps of which are as follows:

[0032] A. Double bond modification of chitosan

[0033] Fully dissolve chitosan in the buffer, and then add glycidyl methacrylate to the buffer, so that the concentration of chitosan in the buffer is 10mg / ml, and the concentration of glycidyl methacrylate is 2mg / ml ; Settling or dialysis after 6h, and then drying to obtain chitosan containing double bond modification;

[0034] B. Preparation of Smart Hydrogels

[0035] B1. Dissolve polydopamine nanoparticles, N-isopropylacrylamide and the double-bonded modified chitosan obtained in step A in deionized water to obtain a mixed solution, and the concentration of polydopamine nanoparticles in the mixed solution is 0.1 mg / ml, the concentration of N-isopropylacrylamide is 80mg / ml, and the concentration of double bond modified chitosan is 200mg / ml;

[0036] B2. Add the cross-linking agent...

Embodiment 2

[0042] A preparation method of an infrared-responsive high-strength hydrogel for cartilage repair, the specific steps of which are as follows:

[0043] A. Double bond modification of carboxymethyl chitosan

[0044] Fully dissolve carboxymethyl chitosan in the buffer so that the concentration of carboxymethyl chitosan in the buffer is 12 mg / ml, and add 0.1 times the molar amount of carboxymethyl chitosan NHS and carboxymethyl chitosan. Sugar 0.1 times the molar amount of EDC; then add glycidyl methacrylate to the buffer, so that the concentration of glycidyl methacrylate in the buffer is 12mg / ml; after 24h sedimentation or dialysis, and then drying, to obtain a Double bond modified carboxymethyl chitosan;

[0045] B. Preparation of Smart Hydrogels

[0046] B1. Polydopamine nanoparticles, N-isopropylacrylamide and the double bond modified carboxymethyl chitosan obtained in step A are dissolved together in the double bond modified carboxymethyl chitosan obtained in step A The ...

Embodiment 3

[0053] A preparation method of an infrared-responsive high-strength hydrogel for cartilage repair, the specific steps of which are as follows:

[0054] A. Double bond modification of chondroitin sulfate

[0055] Fully dissolve chondroitin sulfate in the buffer, and then add methacrylic anhydride to the buffer, so that the concentration of chondroitin sulfate in the buffer is 10 mg / ml, and the concentration of methacrylic anhydride is 60 mg / ml; after 20 hours of dialysis , then dialyzed, freeze-dried to obtain chondroitin sulfate containing double bond modification;

[0056] B. Preparation of Smart Hydrogels

[0057] B1. Polydopamine nanoparticles, N-isopropylacrylamide and the double-bonded modified chondroitin sulfate obtained in step A are jointly dissolved in deionized water to obtain a mixed solution, and the concentration of polydopamine nanoparticles in the mixed solution is 0.5 mg / ml, the concentration of N-isopropylacrylamide is 150mg / ml, and the concentration of do...

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Abstract

The invention discloses a preparation method of an infrared-responsive high-strength hydrogel for cartilage repair. The main operation is to modify polysaccharides or proteins with double bonds through methacrylic acid, methacrylic anhydride, and methacrylate substances, so that the side chains have double bond groups and can be polymerized to form hydrogels; Under the action of initiator and cross-linking agent, N-isopropylacrylamide, double bond modified polysaccharide or protein polymerize in situ to form double network high-strength hydrogel. The infrared-responsive high-strength hydrogel prepared by the method has good biocompatibility and excellent mechanical properties, and has good application prospects in the field of articular cartilage repair.

Description

technical field [0001] The invention relates to a preparation method of an infrared response high-strength intelligent hydrogel for articular cartilage repair. Background technique [0002] Hydrogel has a soft and elastic three-dimensional network structure, which can provide a microenvironment closer to the natural cartilage extracellular matrix for cell proliferation and differentiation, and is an ideal material for cartilage tissue repair. [0003] In the process of promoting cartilage repair, the growth factors traditionally loaded in hydrogels cannot be released in a controlled manner according to the repair requirements, which is an important problem that restricts the application of hydrogels in cartilage repair. In recent years, light-responsive hydrogels have attracted more and more attention because of their intelligence in sensing changes in the external environment and in the release of external stimuli-response control factors. However, the existing light-respo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/22A61L27/20A61L27/18A61L27/50A61L27/52A61L27/54C08L5/08C08L5/04C08L79/02C08J3/24
Inventor 鲁雄张晏宁王振铭韩璐胥杰龙甘东林
Owner SOUTHWEST JIAOTONG UNIV
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