Preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength

A mussel adhesion protein and composite hydrogel technology, which is applied in the field of preparation of PEGDA-mussel adhesion protein-collagen composite hydrogel, can solve the problems of poor adhesion, low mechanical strength and limitations of composite colloidal tissues Application and other issues, to achieve good biological safety, low cost, easy to operate

Active Publication Date: 2020-11-27
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen is also added to PEGDA gel by physical mixing to enhance cell adhesion, but after the two are physically mixed, the mechanical strength does not change significantly, and its mechanical strength is still not high
In addition, the tissue adhesion of the composite colloid is still poor, which is not conducive to the fixation on the tissue surface, limiting its application

Method used

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  • Preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength
  • Preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength
  • Preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation method of PEGDA-mussel adhesion protein-collagen composite hydrogel.

[0032] 1) Dissolve the mussel adhesive protein in 5% acetic acid solution, stir at 4°C for 5 minutes to obtain the mussel adhesive protein acetic acid solution, where the mass volume concentration of mussel adhesive protein is 0.01g / mL;

[0033] 2) Directly dissolve fish skin collagen solids in the mussel adhesion protein acetic acid solution in step 1), stir for 4 hours at 4°C to obtain a mussel adhesion protein-fish skin collagen solution, in which the quality of the fish skin collagen The volume concentration is 1mg / mL;

[0034] 3) Add 4M sodium hydroxide solution dropwise to the mussel adhesion protein-fish skin collagen solution obtained in step 2), and stir to adjust the pH to 9 at 4°C;

[0035] 4) Then, add the PEGDA solid to the mussel adhesion protein-fish skin collagen solution with pH 9 to make the PEGDA mass and volume concentration 0.05g / mL. After it is completely dissolve...

Embodiment 2

[0036] Example 2: Preparation method of PEGDA-mussel adhesion protein-collagen composite hydrogel.

[0037] 1) Dissolve mussel adhesion protein in 5% acetic acid solution, stir at 4°C for 5 minutes to obtain mussel adhesion protein acetic acid solution, wherein the mass volume concentration of mussel adhesion protein is 0.05 g / mL;

[0038] 2) Dissolve the fish skin collagen solid directly in the mussel adhesion protein acetic acid solution and stir at 4°C for 4 hours to obtain the mussel adhesion protein-fish skin collagen solution, in which the mass volume concentration of fish skin collagen is 2 mg / mL;

[0039] 3) Add 4M sodium hydroxide solution dropwise to the mussel adhesion protein-fish skin collagen solution obtained in step 2), and stir to adjust the pH to 7 at 4°C;

[0040] 4) Then, add the PEGDA solid to the mussel adhesion protein-fish skin collagen solution with pH 7 to make the PEGDA mass and volume concentration 0.1g / mL. After it is completely dissolved, add 5mg / mL phot...

Embodiment 3

[0041] Example 3: Preparation method of PEGDA-mussel adhesion protein-collagen composite hydrogel.

[0042] 1) Dissolve the mussel adhesive protein in an acetic acid solution with a volume concentration of 5%, stir at 4°C for 5 minutes to obtain the mussel adhesive protein acetic acid solution, in which the mass volume concentration of the mussel adhesive protein is 0.1g / mL;

[0043] 2) Directly dissolve the methacrylamide collagen solid in the mussel adhesive protein acetic acid solution in step 1), stir at 4°C for 4 hours to obtain the mussel adhesive protein-methacrylamide collagen solution, wherein The mass volume concentration of acrylamide collagen is 4mg / mL;

[0044] 3) Add 4M sodium hydroxide solution dropwise to the mussel adhesion protein-methacrylamide collagen solution obtained in step 2), stir and adjust the pH to 5 at 4°C;

[0045] 4) Then, add the PEGDA solid to the mussel adhesion protein-methacrylamide collagen solution with pH 5 to make the PEGDA mass volume concentr...

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Abstract

The invention discloses a preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength, belonging to the technical field of hydrogel. In order to improve the mechanical strength and tissue adhesion of a collagen hydrogel, the invention discloses the preparation method for the PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength. The method comprises the following steps: dissolving mussel adhesive protein into an acetic acid solution to obtain an acetic acid solution of the mussel adhesive protein; dissolving a collagen solid in the acetic acid solution of the mussel adhesive protein to obtain a mussel adhesive protein-collagen solution; carrying out alkali neutralization until a pH value is in a range of 5-9, and adding a PEGDA solid until the solid is completely dissolved; and adding a photoinitiation factor, and carrying out ultraviolet crosslinking to form thePEGDA-mussel adhesive protein-collagen composite hydrogel. The preparation method is simple in process and easy to operate, and has wide application prospects in the fields of tissue engineering, medicine and the like.

Description

Technical field [0001] The invention belongs to the technical field of hydrogels, and specifically relates to a method for preparing a PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength. Background technique [0002] Collagen is the main component of the extracellular matrix. It has the characteristics of biocompatibility, biodegradability, cell adhesion, low immunogenicity, etc., and has broad applications in drug delivery, skin substitutes, tissue engineering, etc. At present, commercial collagen is mainly derived from pig skin and bovine Achilles tendon, but the high extraction cost and the possibility of virus transmission limit the application of mammalian collagen. Fish skin and other aquatic product processing wastes are one of the sources of collagen, with low price and high safety. Therefore, fish collagen gradually takes a place in the market. However, regardless of whether the collagen is derived from mammals or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/28C08J3/24C08J3/075C08L89/00C08L71/02
CPCC08J3/28C08J3/246C08J3/075C08J2389/00C08J2371/02C08J2489/00C08J2471/02
Inventor 包子娴孙粤年锐咸漠樊喜英陈泉刘文帅彭馨莹
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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