158 results about "Adhesive proteins" patented technology

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component and the clotting and adhesive proteins component are harvested simultaneously from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.

3,4-Dihydroxyphenyl-L-alanine (DOPA) is an unusual amino acid found in mussel adhesive proteins (MAPs) that form tenacious bonds to various substrates under water. DOPA is believed to be responsible for the adhesive characteristics of MAPs. This invention relates to a route for the conjugation of DOPA moieties to various polymeric systems, including but not limited to poly(ethylene glycol) or poly(alkylene oxide) systems such as poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) block copolymers.

The invention provides human extracellular adhesive proteins (EXADH) and polynucleotides which identify and encode EXADH. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of EXADH.

The present invention relates to the discovery that biocompatible anionic polymers can effectively inhibit fibrosis, scar formation, and surgical adhesions. The invention is predicated on the discovery that anionic polymers effectively inhibit invasion of cells associated with detrimental healing processes, and in particular, that the effectiveness of an anionic polymer at inhibiting cell invasion correlates with the anionic charge density of the polymer. Thus the present invention provides a large number of materials for use in methods of inhibiting fibrosis and fibroblast invasion. Anionic polymers for use in the invention include but are not limited to natural proteoglycans, and the glycosaminoglycan moieties of proteoglycans. Additionally, anionic carbohydrates and other anionic polymers may be used. The anionic polymers dextran sulfate and pentosan polysulfate are preferred. In a more preferred embodiment, dextran sulfate, in which the sulfur content is greater than about 10% by weight, may be used. In a more preferred embodiment, the average molecular weight is about 40,000 to 500,000 Daltons. The present invention provides compositions and methods to inhibit fibrosis and scarring associated with surgery. The invention further provides compositions and methods to inhibit glial cell invasion, detrimental bone growth and neurite outgrowth. In a preferred embodiment, the inhibitory compositions further comprise an adhesive protein.

The present invention relates to technology of immobilizing or coating various functional bioactive substances on various surfaces without physical chemical treatment using mussel adhesive protein. More specifically, the present invention relates to a functional scaffold for tissue engineering comprising artificial extracellular matrix, manufactured by coating various functional bioactive substances on the surface of nanofiber and metal scaffold using mussel adhesive protein, and a method of manufacturing the same.

The invention describes new synthetic medical adhesives and films which exploit the key components of natural marine mussel adhesive proteins.

The invention discloses a bioadhesive, which contains a polymer and a solvent, wherein the polymer is a graft of dopamine or mussel adhesive protein and oxidized graft A; the graft A is a graft of a macromolecular substance andan acrylate compound; a six-membered ring in a macromolecular substance in the oxidized graft A is bonded with an aldehyde group; the macromolecular substance is selected from at least one of hyaluronic acid, chondroitin sulfate, gelatin, polypeptide, alginic acid and chitosan; and the acrylate compound is selected from one of methyl methacrylate, methacrylate, glycidyl methacrylate and acrylic ester. The invention further provides a preparation method of the bioadhesive. The bioadhesive has the advantages of being good in biological activity, relatively good in mechanical property and biodegradable, and is capable of promoting tissue healing in a moist environment.

The invention relates to the technical field of cosmetics for skin beautifying, and particularly relates to an application of a mussel adhesive protein in preparing cosmetics for skin beautifying. The application disclosed by the invention has the following main beneficial effects: 1, after a mussel adhesive protein-containing cosmetic is applied on the skin, a molecular layer is formed on the skin, and after most of moisture is evaporated, because the mussel adhesive protein has a strong pyknosis effect, the mussel adhesive protein can promote the uniform shrinkage of the skin, so that the skin forms a uniform and consistent shrinkage layer, and then the occurrence of large wrinkles is avoided, thereby achieving the effect of instant skin firming and wrinkle removal, and improving the firmness and wrinkle resilience of the skin; 2, the smoothness and glossiness of the skin are increased; 3, the action of stains is reduced; and 4, the mussel adhesive protein is non-toxic and harmless.

The invention discloses a preparation method of multifunctional group bionic mussel adhesive protein polymer. The preparation method comprises four processes: the synthesis and preparation of polysuccinimide prepolymer powder, the synthesis and preparation of water soluble polysuccinimide derivative, the synthesis and preparation of polyaspartic acid derivative, and the synthesis and preparation of bionic mussel adhesive protein polymer. The preparation method utilizes the characteristics of environment protection and nontoxicity of polysuccinimide and the characteristics of adhesive protein and organic coating, sufficient catechol groups introduced to a structure can perform complexation with metal ion on the surface of metal base materials to form a passive film, so that good adhesion is formed on the surface of the metal base materials, active epoxy groups and hydroxyl easily cured and crossly bonded with the organic coating are introduced into the molecular structure, the interface chemical bonding probability between the polymer and the organic coating is improved, the adhesive strength between the polymer and the organic coating is realized, and the protective performance of the whole protective coating is improved.

The present invention comprises a Mytilus edulis cDNA sequenc having a nucleotide sequence that encodes for the Mytilus edulis foot protein-1 (Mefp-1), an example of a mollusk foot protein. Mefp-1 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-1 gene will allow researchers to produce Mefp-1 protein using genetic engineering techniques. The discovery of Mefp-1 gene sequence will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.

The present invention relates to a bio-adhesive derived from mussel. In particular, it relates to a recombinant protein fp(foot protein)-131 that is a hybrid of fp-3 variant A and fp-1. According to the present invention, the recombinant protein with adhesive activity can be economically produced in large scale to be used in place of chemical adhesives.

The invention describes new synthetic medical adhesives and antifouling coatings which exploit the key components of natural marine mussel adhesive proteins.

The invention provides a method for preparing a marine mussel adhesive protein waterproof biological adhesive, and relates to a biological adhesive. The invention provides the method for preparing marine mussel-Mytilus coruscus adhesive protein waterproof biological adhesive with degradability, water resistance and good biological compatibility by a method of biological gene engineering. The method comprises the following steps: extracting total RNA from the foot part of alive Mytilus coruscus to be reversely transcribed into cDNA; designing a PCR primer according to an Mcfp-1 gene sequence of the Mytilus coruscus; connecting the obtained DNA fragment in a prokaryotic expression vector pGEX-4T through PCR amplification to construct a recombinant plasmid; then transforming the recombined plasmid in a colon bacillus BL21 bacterial strain; and finally, purifying and modifying the expressed polypeptide product. The preparation method is simple, and the expression product is soluble, can be easily purified with low cost, and has similar or better effect compared with CELL-TAK<TM> cell and tissue adhesive without cytotoxicity. The adhesive accords with the requirement of scientific research, and can be used as a biological medical material.

A preparation method for high-performance water-based environmentally-friendly anticorrosive paint used for a metal base material comprises the step that a component A and a component B are blended with the ratio being 1: (0.3-1.2), and then the high-performance water-based environmentally-friendly anticorrosive paint can be prepared, wherein the component A comprises, by weight, 30-45 parts of waterborne epoxy curing agents, 10-15 parts of non-heavy-metal antirust paint, 11-28 parts of de-ionized water, 15-30 parts of packing, 2-7 parts of thickening agents, 0.3-1.3 parts of dispersing agents, 0.1-0.5 part of flatting agent, 0.1-0.4 part of de-foaming agent, 0.1-0.8 part of PH conditioning agent and 1-4 parts of film coalescing aid; the component B comprises, by weight, 85-95 parts of water-borne epoxy resin and 5-15 parts of mussel adhesive proteins. The mussel adhesive proteins in the paint are utilized to perform a strong coordination complexation effect with ions in the non-heavy-metal antirust paint, and the adhesive force of a coating to the metal base material is increased to 10 MPa; at the temperature of 25 DEG C, the water resistance reaches 1,400 h, the salt fog resistance reaches 2,000 h, and coated films do not foam or rust.

The present invention relates to a bioadhesive derived from mussel. In particular, it relates to a novel MGFP-3A MUTANT (Mytilus galloprovincialis foot protein type-3A MUTANT) protein and a recombinant protein that is a hybrid of MGFP-3A MUTANT, FP (Foot Protein)-1 and MGFP-5 (Mytilus galloprovincialis foot protein type-5). According to the present invention, the adhesive protein can be economically produced in large scale and can be used instead of chemical adhesives.

It is intended to provide a novel method of immobilizing a protein and a protein chip, by which the protein can be immobilized at a high reproducibility while preventing the protein from inactivation without resort to a large-scaled apparatus and the protein can be immobilized even in a microchannel. Further, by using a cell adhesive protein as the protein to be immobilized, it is also possible to use a cell as a target and to provide a method of immobilizing a cell and a cell chip, by which a cell can be immobilized in an arbitrary region on a substrate.

The invention provides a material for promoting cell growth adhering to a wall and a preparation method of the material. The material for promoting cell growth adhering to wall comprises a solid medium used for cell culture and a mussel adhesive protein adhered to the solid medium; the preparation method of the material comprises the following steps: adding a mussel adhesive protein solution into a culture vessel or a suspension culture carrier and automatically adhering the mussel adhesive protein to the solid surface for use. The method and the material provided by the invention can promote the cell to fast adhere to the wall and crawl; and the material can be preserved at normal temperatures, can tolerate sterilization of gamma rays and reduce the usage amount of supernatant serum and is especially applied to the serum-free culture environment.

The invention describes new synthetic medical adhesives and antifouling coatings which exploit the key components of natural marine mussel adhesive proteins.

An assay article for detection first biomolecules contained in a sample is described. The assay article includes a substrate having a modified surface and a first biomolecule directly adsorbed and immobilized on the modified surface of the substrate without linking moieties. A second biomolecule is bound to or adsorbed on the first biomolecule. Also disclosed is a method of making the assay article. A first biomolecule (other than an adhesive protein) is contacted with a modified surface of a substrate. The substrate is dried to directly adsorb the first biomolecule and immobilize it on the modified surface of the substrate without additional fixing steps to form an activated substrate. Then, a second biomolecule is contacted with the activated substrate under conditions sufficient for the first biomolecule to bind the second biomolecule.

The invention provides an antimicrobial coating composition, which consists of mussel adhesive protein and silver nanoparticles. The invention also provides a medical implant material containing an antimicrobial coating and a preparation method of the medical implant material. The antimicrobial coating formed by the composition disclosed by the invention, as an antimicrobial coating which is excellent in performance and is safe and reliable, is quite excellent in biocompatibility and antimicrobial activity, free from toxic and side effects and free from rejection reaction. The antimicrobial coating containing medical implant material provided by the invention is strong in antimicrobial activity, good in biocompatibility and is applicable to various substrate materials, and the medical implant material is simple in manufacturing process and strong in universality; and the medical implant material is capable of achieving mass production and clinical application, and the implant material has important social and economic significances.

The invention relates to an aquagel material used to recover hurt nerve center. Wherein, it uses nerve center cross method to graft the layer adhesive protein (Laninin) with nerve grow activity to the molecule skeleton of transparent acid aquagel, to rpare the aquagel frame, which can accelerate the regeneration of nerve axon.