16 results about "Adhesive proteins" patented technology

The application discloses a Mycoplasma bovum adhesion protein FtsZ and application thereof. The nucleic acid sequence of the Mycoplasma bovum adhesion protein FtsZ is shown as SEQ ID NO. 1. The application also discloses a recombinant plasmid pET-30a-ftsZ and an E. coli containing the recombinant plasmid pET-30a-ftsZ. ftsZ The recombinant protein rFtsZ has the advantages of being capable of specifically combining with EBL cell membrane protein, being capable of combining with extracellular matrix components (fibronectin, fibronectin, laminin and type IV collagen), having the direct adhesion host cell effect, having good antigenicity, being capable of producing high-level antibodies, being capable of providing good immune protection effect, and providing a new target and thought for elucidating the pathogenic mechanism of the Mycoplasma bovum and developing a new vaccine and medicine.

This invention relates to recombinant mussel adhesive proteins, the nucleic acid molecules encoding them, nucleic acid constructs, expression vectors, recombinant engineered strains, pharmaceutical compositions, preparation methods, purification methods, and applications. This invention assembles and splices partial functional sequences from different mussels and type III collagen to obtain several novel mussel adhesive protein sequences. Further screening yielded recombinant mussel adhesive proteins Mfp5, Mfp55, Mfp131, and Mfp151, suitable for large-scale industrial production and exhibiting high expression levels. Efficacy experiments demonstrated that these proteins significantly promote cell migration, indicating their excellent cell repair function and broad application prospects.

The application discloses a method for constructing a model for recognizing mussel adhesive proteins, and belongs to the technical field of bioinformatics, and specifically comprises the following steps: inputting an amino acid sequence of a target protein, obtaining standard electromagnetic response waveforms of each amino acid residue in a variety of non-aqueous solvent environments, and sorting to form an initial electromagnetic fingerprint; polypeptide fragment fingerprints are generated through sliding window cutting, shared frequency components of each fragment waveform are extracted, and characteristic sequences are formed by sorting according to intensity; local patterns and context dependencies of the sequences are extracted through an encoder network, and a context vector is output; a decoder network maps the vector into a fixed-dimension embedding representation; known protein sequences with or without adhesive functions are used as training samples, the above-mentioned processing is performed on each sample to obtain a set of embedding representations, a binary classification model is trained, and a recognition model is obtained. The application realizes function recognition by using electromagnetic response characteristics of proteins in non-aqueous environments.

This invention provides a method for preparing a thermosensitive gel for the prevention and treatment of nasal diseases and its application. By weight and volume percentage, it comprises recombinant human type III collagen, mussel adhesive protein, an anti-allergy agent, traditional Chinese medicine extracts, thermosensitive materials, and a sodium pyruvate-cocamidopropyl betaine ion-pair complex. This invention, by pre-forming the ion-pair complex, simultaneously solves the technical problems of existing nasal gels, such as poor anti-dilution ability, short adhesion time, and easy inactivation of active ingredients. The product is liquid below 30°C and can be sprayed for complete nasal coverage. At body temperature, it transforms into a gel within 2 minutes for long-lasting adhesion, exhibiting rapid anti-inflammatory, nasal patency-clearing, antipruritic, and mucosal repair effects. It is suitable for the prevention and treatment of allergic rhinitis and sinusitis, as well as nasal wound care, with significantly better efficacy than similar commercially available products.

The application discloses a biological macromolecular drug for tumor treatment, which has tissue protein adhesion, can continuously improve the oxidative stress environment of a tumor site by prolonging the time at the tumor site, can inhibit tumor growth by tumor microenvironment regulation, can also be used as an auxiliary therapeutic agent to enhance the curative effect of other tumor treatments, and has a good clinical transformation prospect.

This invention belongs to the field of dressing preparation technology, specifically relating to a mussel protein dressing with anti-inflammatory, antibacterial, moisturizing, repairing, and wound-healing effects, and its preparation method. The invention comprises the following raw materials by weight: 15-25 parts mussel adhesive protein ferment, 2-5 parts reed extract, 6-10 parts geranium extract, 4-8 parts mannitol, 10-15 parts glycerin, 0.6-1 part sodium chloride, 0.2-0.5 parts p-hydroxyacetophenone, 1-2 parts 1,2-hexanediol, 0.05-0.1 parts ectoine, and 100-200 parts water. This invention utilizes a specific combination of mussel adhesive protein ferment, reed extract, and geranium extract to facilitate the synergistic effect of each active substance and fully release its efficacy. Therefore, the technical solution of this invention has the advantages of more comprehensive functions and more significant effects, enabling anti-inflammatory, antibacterial, moisturizing, repairing, and wound-healing effects.

This invention belongs to the field of biomedical technology and provides a mussel adhesive protein and azelastine hydrochloride nasal thermosensitive gel, its preparation and application. The mussel adhesive protein and azelastine hydrochloride nasal thermosensitive gel includes an active ingredient and pharmaceutically acceptable excipients. The active ingredient is recombinant mussel adhesive protein mefp-5 subtype and azelastine hydrochloride. The excipients include a thermosensitive polymer, which is mixed with the active ingredient to form a thermosensitive gel system. This invention solves the technical problem that existing single-drug formulations cannot simultaneously provide rapid symptom relief and long-term mucosal barrier repair for allergic rhinitis by combining recombinant mussel adhesive protein mefp-5 subtype with mucosal repair function with azelastine hydrochloride, which has rapid anti-allergic effects. Pharmacodynamic studies have confirmed that the two active ingredients exhibit a significant synergistic effect (CDI < 0.7) in improving allergy symptoms, inhibiting inflammatory responses, and repairing the mucosal barrier.

This invention relates to the field of bone repair scaffold technology, specifically to a multi-level sustained-release biomimetic bone repair scaffold based on mussel adhesive protein and its preparation method. This invention organically integrates the strong wet adhesion of recombinant mussel adhesive protein, the excellent mechanical properties of biomimetic mineralized collagen, the programmed drug release capability of drug-loaded PLGA-PEG biphase sustained-release microspheres, and the 4D shape memory characteristics of thermosensitive poly(N-isopropylacrylamide) hydrogel to achieve multi-functional synergy. Then, low-temperature deposition 3D printing combined with thermosensitive materials is used to achieve 4D printing of the scaffold, resulting in a scaffold with a complex external morphology and a precise internal porous structure. The mechanical strength reaches the level of cortical bone, and it can actively adapt to the defect morphology after implantation, reducing intraoperative trimming time by more than 50%.

This invention belongs to the field of medical materials technology and discloses a medical composite dressing for relieving dermatitis and eczema and its preparation method. First, mussel adhesive protein is dissolved in purified water, and the pH is adjusted to obtain a mussel adhesive protein solution. Trehalose, ectoine, and a portion of glycerin are mixed and dissolved to obtain an active ingredient composite solution. Then, the mussel adhesive protein solution and the active ingredient composite solution are mixed, and the pH is adjusted to obtain phase A. Sodium carboxymethyl cellulose is dissolved in a buffer solution in batches to obtain sodium carboxymethyl cellulose solution A and sodium carboxymethyl cellulose solution B. The two solutions are mixed, and the remaining glycerin and methylparaben are added. After adjusting the pH, phase B is obtained. Finally, phase A is added dropwise to phase B, stirred, and allowed to stand for maturation to obtain the final product. The medical composite dressing provided by this invention has a unique temperature-pH response mechanism, enabling the release of active ingredients such as ectoine and trehalose under weakly acidic conditions, achieving targeted repair and moisturizing of the inflamed area.

This application relates to the development and application of a novel protein hair dye. This invention discloses a biological hair dyeing system involving three color proteins, mussel adhesive protein, and split intein, and its application, belonging to the field of biotechnology. This invention uses Escherichia coli as the chassis cell to construct and express a recombinant protein functional module mediated by split intein: three color proteins (red, yellow, and blue) connected to the C-terminus of the split intein, and a mussel adhesive protein connected to the N-terminus of the split intein. The key points of its technical solution include: (1) using red, yellow, and blue primary color proteins to achieve multi-tone and full-spectrum color coverage through precise compounding in different proportions; (2) using the excellent underwater interfacial adhesion ability of mussel adhesive protein to firmly anchor the color-developing module to the surface of hair fibers; (3) using the specific interaction between split inteins and protein trans-splicing reaction to achieve covalent connection between the color-developing module and the adhesive module at the molecular level. This invention effectively overcomes the technical bottlenecks of existing microbial pigments, such as monotonous colors and incomplete color gamut. It has the advantages of rich and controllable color development, mild hair dyeing conditions, excellent color fixing performance, and high biological safety, and has broad application prospects in the field of green cosmetics.

This invention discloses a biomimetic recombinant V-type mussel adhesive protein, its preparation method, and its applications. The recombinant V-type mussel adhesive protein is composed of the Mfp-5 protein from natural mussels and proteins that constitute the fibrous skeleton of mussel byssal fibers. During preparation, the fusion gene and a tyrosinase gene are co-expressed, and the expressed tyrosinase is used to modify the tyrosine residues in the recombinant mussel adhesive protein with dopa, thereby obtaining a bioactive recombinant V-type mussel adhesive protein with high yield, high purity, and low endotoxin content, fully meeting the application requirements of pharmaceuticals, medical devices, and cosmetics.

This invention discloses a production quality control method for medical mussel adhesive protein nasal spray, belonging to the field of medical preparation production and quality management technology. The method acquires measured data on the physicochemical parameters of each process in historical batches, as well as the molecular aggregation state spectrum and oxidative crosslinking activity state of the finished product, and constructs a two-way control sample set corresponding to each batch. A multivariate gradient coupling fitting algorithm is used to construct a mapping model between physicochemical parameters and functional states. Real-time physicochemical parameters of the current production batch are input, and a hidden state time-series extrapolation algorithm is used to output dynamic evolution data and determine the quality level. Data from multiple batches are integrated to form a batch quality traceability chain. For batches with abnormal deviations, the production process is adjusted and the extrapolation is repeated until the functional state deviation converges. This invention achieves continuous monitoring and dynamic quality management of the production process of medical mussel adhesive protein nasal spray by mapping physicochemical parameters to functional states and combining time-series extrapolation and batch traceability.

The application discloses a mussel adhesive protein biomimetic copolymer modified asphalt and a preparation method thereof. The modified asphalt comprises matrix asphalt and a biomimetic copolymer formed by copolymerization of catechol structural units and a free radical initiating monomer. The biomimetic copolymer can form a dynamic network in the asphalt system through hydrogen bonding, pi-pi interaction and metal coordination, and form a strong adhesion layer on the aggregate surface, thereby significantly improving the adhesion and water damage resistance of the asphalt. At the same time, the free radical initiating monomer can react with asphalt aging free radicals under ultraviolet irradiation, effectively terminating the aging process and imparting excellent ultraviolet aging resistance to the modified asphalt. The preparation method is simple, suitable for industrial production, and particularly suitable for road engineering in the hot and humid regions of South China.

The invention relates to the technical field of biology, and particularly discloses fungus and diatom symbiont microspheres for improving the immune function of cage culture stichopus japonicus and improving water quality. Core functional components of the fungus and diatom symbiont microspheres are cordyceps militaris fungi and Ampelopsis coffee, and in addition, in order to improve the biological activity of the symbiont microspheres and the adhesion of the symbiont microspheres to a netting, pullulan, recombinant mussel mucin, a folium cortex eucommiae extract and a curcuma zedoary extract are also added in the preparation process of the microspheres. The functional components of the symbiont microsphere disclosed by the invention are mutually beneficial, common and synergistic, the non-specific immune function of the stichopus japonicus can be comprehensively improved by enhancing the activity of immune enzyme and digestive enzyme, and meanwhile, the contents of nitrogen phosphorus, nitrite, organic pollutants and pathogenic vibrio in an aquaculture water body can also be reduced.