41 results about "Adhesive proteins" patented technology

The invention relates to the technical field of repair compositions, and discloses a repair composition based on mussel mucin and application of the repair composition in skin barrier repair. The invention discloses a mussel mucoprotein-zinc composite microcapsule repair agent, which is prepared from the following components in parts by weight: mussel mucoprotein-zinc composite microcapsules, modified carboxymethyl chitosan, isolactone palmitate, a pH response type ceramide slow release system, dimeticone, cetostearyl alcohol, a calcium ion activating agent, an auxiliary repair factor, beheneth-25, caprylic / capric triglyceride, glyceryl monostearate, glyceryl distearate, pentanediol and citric acid. The preparation method comprises the following steps: adding ethanol, phenoxyethanol / ethylhexylglycerin, trehalose, beta-glucan and purified water; according to the whole technical scheme, through functional complementation and process optimization of all the components, synergistic interaction is achieved, the transpedermis water loss amount can be rapidly reduced, the water content of the skin cuticle is increased, the damaged skin barrier structure can be repaired from the source, and the resistance and stability of the skin are remarkably improved.

The application provides a mussel mucin synthesis method and relates to the technical field of genetic engineering.The application improves the yield of mussel mucin by carrying out deletion mutation on the N-terminal sequence of the secondary structure of the mussel mucin amino acid sequence such as SEQ ID NO.1.The yield of mussel mucin in the prior art is about 2.57g / L, while the yield of mussel mucin in the application is about 5g / L, which is greatly improved compared with the yield of mussel mucin in the prior art.On this basis, the mussel mucin is modified by dopa through co-expression of tyrosinase, and the expression amount of the tyrosinase is controlled through different intensities of RBS, so as to regulate the modification degree of dopa, improve the yield of mussel mucin, and realize controllable modification of dopa in the mussel mucin, finally obtaining mussel mucin with high yield and different modification degrees of dopa.

The invention discloses a special membrane material for targeted trapping of macrobrachium larvae in water and a preparation method of the special membrane material, and belongs to the field of water environment biological pollution prevention and control. The membrane material is of a multi-layer composite structure and comprises a nanofiber membrane base material layer, an interface adhesion layer and a functional modification layer. The interface adhesion layer is bionic coacervate gel formed by curing hydrophobic polyelectrolyte and phytic acid; the functional modification layer is covalently grafted with a bionic adhesion protein active peptide fragment and a macrobrachium larva chemical pheromone. The material actively attracts larvae to approach through chemical pheromones, high-strength adhesion capture is achieved through bionic peptide fragments rich in aromatic and cationic amino acids, and trapping is integrated. The preparation method comprises the steps of electrostatic spinning of a base material, surface activation, gel coating, functional modification and the like. The material disclosed by the invention has high selectivity and high capture rate on the larvae of the Limnoperna fortunei, is environment-friendly, long-acting and stable, and is suitable for green prevention and treatment of fouling of the larvae of the Limnoperna fortunei in facilities such as water pipelines and pump stations.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

The invention relates to the technical field of medical biological materials, and discloses a mussel mucin-chondroitin sulfate composite hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: respectively preparing a mussel mucin aqueous solution and a chondroitin sulfate aqueous solution; the mass volume concentration of the mussel mucoprotein aqueous solution is 1%-10%, the mass volume concentration of the chondroitin sulfate aqueous solution is 1%-10%, the mass ratio of the mussel mucoprotein to the chondroitin sulfate is 3: 1-1: 3, the two solutions are mixed and stirred or oscillated under the conditions that the temperature is 4-37 DEG C and the pH value is 7.0-9.0, a cross-linking reaction is conducted, and hydrogel is formed; the cross-linking mode is physical cross-linking, chemical cross-linking or physical-chemical dual cross-linking; according to the invention, excellent tissue adhesion, injectability, self-healing property and biodegradability are realized, cell adhesion and proliferation can be effectively promoted, and the gel is suitable for various medical beauty scenes such as skin filling, wound repairing and anti-aging nursing.

The application discloses a Mycoplasma bovum adhesion protein FtsZ and application thereof. The nucleic acid sequence of the Mycoplasma bovum adhesion protein FtsZ is shown as SEQ ID NO. 1. The application also discloses a recombinant plasmid pET-30a-ftsZ and an E. coli containing the recombinant plasmid pET-30a-ftsZ. ftsZ The recombinant protein rFtsZ has the advantages of being capable of specifically combining with EBL cell membrane protein, being capable of combining with extracellular matrix components (fibronectin, fibronectin, laminin and type IV collagen), having the direct adhesion host cell effect, having good antigenicity, being capable of producing high-level antibodies, being capable of providing good immune protection effect, and providing a new target and thought for elucidating the pathogenic mechanism of the Mycoplasma bovum and developing a new vaccine and medicine.

The invention belongs to the technical field of medical dressings, and particularly relates to a gel dressing for relieving postoperative lymphedema of breast cancer and a preparation method thereof.The gel dressing is prepared from, by weight, 0.1-0.5 part of active medicine, 8-12 parts of modified matrix, 0.2-0.8 part of dopamine hydrochloride, 2 parts of dispersing agent and 0.5-1.5 parts of enhancer, menthol with functions of promoting infiltration and inducing diuresis to alleviate edema is used for modifying mussel mucoprotein with good biocompatibility, the crosslinking gelling property of the mussel mucoprotein is improved, meanwhile, along with hydrolysis breakage of covalent bonds, gel slowly collapses to release active drugs, and the active drugs and the menthol cooperate with each other, and the postoperative lymphedema of breast cancer is treated from various mechanism ways.

1. The name of the design product: packaging box (multiple-factor medical mussel mucin liquid dressing). 2. The use of the design product: for packaging box. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

An antiviral coating composition is provided. An antiviral coating composition according to one embodiment of the present invention is implemented by including an antiviral component comprising an antiviral fusion protein in which an antiviral motif is bound to an adhesive protein. According to the present invention, the composition has excellent processability enabling easy provision on various surfaces of various products, has adhesion sustainability enabling an adhesive state to be maintained for a long period of time after being adhered to a surface, and has activity sustainability enabling antiviral activity to be maintained for a long period of time without a loss in activity according to external conditions during preparation, storage and use.

This invention relates to recombinant mussel adhesive proteins, the nucleic acid molecules encoding them, nucleic acid constructs, expression vectors, recombinant engineered strains, pharmaceutical compositions, preparation methods, purification methods, and applications. This invention assembles and splices partial functional sequences from different mussels and type III collagen to obtain several novel mussel adhesive protein sequences. Further screening yielded recombinant mussel adhesive proteins Mfp5, Mfp55, Mfp131, and Mfp151, suitable for large-scale industrial production and exhibiting high expression levels. Efficacy experiments demonstrated that these proteins significantly promote cell migration, indicating their excellent cell repair function and broad application prospects.

The application discloses a method for constructing a model for recognizing mussel adhesive proteins, and belongs to the technical field of bioinformatics, and specifically comprises the following steps: inputting an amino acid sequence of a target protein, obtaining standard electromagnetic response waveforms of each amino acid residue in a variety of non-aqueous solvent environments, and sorting to form an initial electromagnetic fingerprint; polypeptide fragment fingerprints are generated through sliding window cutting, shared frequency components of each fragment waveform are extracted, and characteristic sequences are formed by sorting according to intensity; local patterns and context dependencies of the sequences are extracted through an encoder network, and a context vector is output; a decoder network maps the vector into a fixed-dimension embedding representation; known protein sequences with or without adhesive functions are used as training samples, the above-mentioned processing is performed on each sample to obtain a set of embedding representations, a binary classification model is trained, and a recognition model is obtained. The application realizes function recognition by using electromagnetic response characteristics of proteins in non-aqueous environments.

1. Name of the designed product: packing box (mussel adhesive protein anorectal dressing). 2. Use of the designed product: for packing products. 3. Design points of the designed product: in the combination of shape and pattern. 4. Picture or photo best indicating the design points: front view.

The invention provides a layered graphene / epoxy resin composite coating and a preparation method thereof. The preparation method comprises the following steps: pretreating the surface of mussel mucoprotein (MAP), and alternately and repeatedly preparing an EP film and a graphene layer by adopting EP film spin-coating and graphene self-assembly based on a Marangoni method to obtain a Lay-GEC coating. According to the layered graphene / epoxy resin composite coating and the preparation method, self-assembly and horizontal directional arrangement of the graphene nanosheets on the water surface are achieved through the Marangoni effect, and the compactness and barrier performance of the coating are improved. And the catechol group of the mussel protein is utilized to realize the spontaneous coordination repair of the scratched part, so that the self-repair capability and durability of the coating are improved.

The invention relates to a recombinant mussel mucin MFP535. The invention further provides a coding nucleic acid, a recombinant vector, a recombinant strain and the like for highly expressing the MFP535, so that the efficient expression of the recombinant mussel mucin MFP535 is realized. The invention further provides a purification and separation method for the recombinant mussel mucin MFP535, the purity of the MFP535 finally obtained through purification reaches 96% or above, the tyrosine oxidation rate reaches 0.44%, and the endotoxin is lt; 1 EU / mg. The recombinant mussel mucin MFP535 has an obvious effect of promoting cell growth. The separation and purification method disclosed by the invention is high in specificity, mild in operation condition, simple in step, good in purification effect, high in recovery rate, low in cost, high in speed and beneficial to expanded production.

This invention provides a method for preparing a thermosensitive gel for the prevention and treatment of nasal diseases and its application. By weight and volume percentage, it comprises recombinant human type III collagen, mussel adhesive protein, an anti-allergy agent, traditional Chinese medicine extracts, thermosensitive materials, and a sodium pyruvate-cocamidopropyl betaine ion-pair complex. This invention, by pre-forming the ion-pair complex, simultaneously solves the technical problems of existing nasal gels, such as poor anti-dilution ability, short adhesion time, and easy inactivation of active ingredients. The product is liquid below 30°C and can be sprayed for complete nasal coverage. At body temperature, it transforms into a gel within 2 minutes for long-lasting adhesion, exhibiting rapid anti-inflammatory, nasal patency-clearing, antipruritic, and mucosal repair effects. It is suitable for the prevention and treatment of allergic rhinitis and sinusitis, as well as nasal wound care, with significantly better efficacy than similar commercially available products.

The application discloses a kind of polypeptide, underwater adhesive, adhesive coating and its preparation method and application, the amino acid sequence of the polypeptide is Lys-DOPA-Phe-Lys-DOPA-Phe.The underwater adhesive prepared by the polypeptide of the application and the adhesive coating formed have considerable underwater adhesion, and have certain resistance to acidic environment and salt environment.In addition, since the polypeptide only contains 6 natural amino acids, compared with natural marine biological adhesive protein containing dozens of hundreds of amino acids, the polypeptide and underwater adhesive of the application have simple sequence, simple and economical synthesis.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.

1. Name of the product in this design: Packaging Box (Recombinant Mussel Adhesive Protein Medical Dressing). 2. Purpose of this design: Packaging box for medical dressings. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The right view is the same as the left view, so the right view is omitted.

The application discloses a biological macromolecular drug for tumor treatment, which has tissue protein adhesion, can continuously improve the oxidative stress environment of a tumor site by prolonging the time at the tumor site, can inhibit tumor growth by tumor microenvironment regulation, can also be used as an auxiliary therapeutic agent to enhance the curative effect of other tumor treatments, and has a good clinical transformation prospect.

The invention relates to the technical field of medical biological materials, and discloses mussel mucoprotein-agarose composite hydrogel and a preparation method and application thereof.The preparation method comprises the steps that agarose powder is dispersed in an aqueous solvent and heated to 80-100 DEG C to be completely dissolved, and a clear agarose solution is obtained; cooling the agarose solution to 40-60 DEG C, and keeping the agarose solution in a liquid state; the preparation method comprises the following steps: dissolving mussel mucin powder in an aqueous solvent or a buffer solution to prepare a mussel mucin solution, and slightly heating (1t, 40 DEG C) to promote dissolution; uniformly mixing the agarose solution obtained in the step S2 and the mussel mucoprotein solution obtained in the step S3 at 40-60 DEG C, and avoiding introducing bubbles to obtain a mixture; cooling the mixture to room temperature or body temperature to form composite hydrogel; carrying out irradiation sterilization on the composite hydrogel; the hydrogel is simple to prepare, has the thermal reversible gel characteristic, has good tissue adhesion and biocompatibility, and can effectively maintain the biological activity of the two natural components.

1. Name of the product in this design: Packaging kit (Medical mussel adhesive protein care solution). 2. Purpose of this design: for product packaging. 3. The key design feature of this product is its pattern. 4. The image or photograph that best illustrates the design highlights: the main view of Suite 2. 5. Other situations requiring explanation: This design product is a kit product, so it is used and sold as a set. Kit 1 is a packaging bottle, and kit 2 is a packaging box. Kit 1 is placed inside kit 2 for sale and use.

The invention provides a composition for promoting wound healing and a preparation method thereof, and belongs to the technical field of medicines. Comprising the following raw materials in parts by weight: 3-5 parts of pseudo-ginseng, 1-3 parts of turmeric, 3-7 parts of astragalus membranaceus, 2-5 parts of herba epimedii, 3-5 parts of rhizoma bletillae, 45-60 parts of lecithin, 10-15 parts of cholesterol, 2-4 parts of ginseng, 0.5-1 part of a surfactant, 0.1-0.2 part of protein peptide, 10-15 g of agarose, 0.2-0.3 part of tetramethylpiperidine oxide, 1-1.5 parts of sodium bromide, 2-4 parts of mussel mucin and 0.1-0.2 part of zinc citrate. The composition is safe, free of side effects, free of chemical cross-linking agent addition, biodegradable, capable of effectively preventing wound reinfection, achieving intelligent drug delivery and promoting rapid healing of wounds, suitable for wound repair in various scenes and wide in application range.

This invention discloses a method for modifying the structure of recombinant mussel adhesive protein, comprising the following steps: S1. Optimizing the mussel adhesive protein gene sequence and cloning it into an expression vector to construct a recombinant plasmid; S2. Transforming the recombinant plasmid into competent cells and culturing them to obtain engineered bacteria; S3. Fermenting the engineered bacteria, collecting the bacterial cells by centrifugation, adding a lysis solution to break the bacterial cells, purifying and collecting the eluent, and dialysis to obtain a dialysate containing non-adhesive recombinant mussel adhesive protein; S4. Adding a chemical oxidant and an enhancer to the dialysate containing the non-adhesive recombinant mussel adhesive protein to react and obtain a highly adhesive recombinant mussel adhesive protein. This invention provides a novel chemical structure modification method for recombinant mussel adhesive protein. Through the synergistic effect of highly selective oxidants and enhancers, the tertiary structure of the protein is unfolded and the reaction activation energy is reduced, achieving efficient and simultaneous tyrosine hydroxylation and DOPA quinone crosslinking. The modification rate is more than 7 times higher than that of traditional chemical methods.

This application relates to a long-lasting antioxidant flavored tomato sauce and its preparation method. The flavored tomato sauce is composed of tomato pulp, emulsifier, flavoring agent, nano-encapsulated antioxidant particles, and water. The nano-encapsulated antioxidant particles consist of a coating liquid, an adsorbent carrier, and an antioxidant dispersion. The coating liquid consists of polysaccharide, water-soluble mussel adhesive protein, and water. The adsorbent carrier is composed of one or more of dietary fiber powder, chitosan fiber powder, and porous starch. The antioxidant dispersion consists of a natural antioxidant, polysorbate-80, and a diluent. By combining polysaccharide with water-soluble mussel adhesive protein, and combining it with the coating carrier and antioxidant dispersion, the resulting nano-encapsulated antioxidant particles, after spray drying, have a slow-release effect. This slow-release antioxidant works synergistically with lycopene and protects the structure of lycopene and vitamin C, preventing obvious oxidation, discoloration, and layering during the tomato sauce's shelf life. This avoids mistaking spoilage for deterioration and causing waste.

This invention belongs to the field of dressing preparation technology, specifically relating to a mussel protein dressing with anti-inflammatory, antibacterial, moisturizing, repairing, and wound-healing effects, and its preparation method. The invention comprises the following raw materials by weight: 15-25 parts mussel adhesive protein ferment, 2-5 parts reed extract, 6-10 parts geranium extract, 4-8 parts mannitol, 10-15 parts glycerin, 0.6-1 part sodium chloride, 0.2-0.5 parts p-hydroxyacetophenone, 1-2 parts 1,2-hexanediol, 0.05-0.1 parts ectoine, and 100-200 parts water. This invention utilizes a specific combination of mussel adhesive protein ferment, reed extract, and geranium extract to facilitate the synergistic effect of each active substance and fully release its efficacy. Therefore, the technical solution of this invention has the advantages of more comprehensive functions and more significant effects, enabling anti-inflammatory, antibacterial, moisturizing, repairing, and wound-healing effects.

This invention belongs to the field of biomedical technology and provides a mussel adhesive protein and azelastine hydrochloride nasal thermosensitive gel, its preparation and application. The mussel adhesive protein and azelastine hydrochloride nasal thermosensitive gel includes an active ingredient and pharmaceutically acceptable excipients. The active ingredient is recombinant mussel adhesive protein mefp-5 subtype and azelastine hydrochloride. The excipients include a thermosensitive polymer, which is mixed with the active ingredient to form a thermosensitive gel system. This invention solves the technical problem that existing single-drug formulations cannot simultaneously provide rapid symptom relief and long-term mucosal barrier repair for allergic rhinitis by combining recombinant mussel adhesive protein mefp-5 subtype with mucosal repair function with azelastine hydrochloride, which has rapid anti-allergic effects. Pharmacodynamic studies have confirmed that the two active ingredients exhibit a significant synergistic effect (CDI < 0.7) in improving allergy symptoms, inhibiting inflammatory responses, and repairing the mucosal barrier.

The application discloses a kind of special membrane material for targeting trapping Corbicula fluminea larvae in water and a preparation method thereof, and belongs to the field of water environmental biological pollution prevention and control.The membrane material is a multilayer composite structure, including a nanofiber membrane substrate layer, an interface adhesion layer and a functional modification layer.The interface adhesion layer is a biomimetic condensed layer gel formed by hydrophobic polyelectrolyte and phytic acid solidification;The functional modification layer is covalently grafted with a biomimetic adhesion protein active peptide segment and a Corbicula fluminea larva chemical information substance.The material actively lures larvae by chemical information substance, and realizes high-strength adhesion capture by using a biomimetic peptide segment rich in aromatic and cationic amino acids, and is integrated with "trapping body".The preparation method includes the steps of electrospinning substrate, surface activation, coating gel and functional modification, etc.The material has high selectivity and high capture rate for Corbicula fluminea larvae, and is environmentally friendly, long-acting and stable, and is suitable for green prevention and control of Corbicula fluminea larvae pollution in facilities such as water conveying pipeline and pump station.

This invention relates to the field of bone repair scaffold technology, specifically to a multi-level sustained-release biomimetic bone repair scaffold based on mussel adhesive protein and its preparation method. This invention organically integrates the strong wet adhesion of recombinant mussel adhesive protein, the excellent mechanical properties of biomimetic mineralized collagen, the programmed drug release capability of drug-loaded PLGA-PEG biphase sustained-release microspheres, and the 4D shape memory characteristics of thermosensitive poly(N-isopropylacrylamide) hydrogel to achieve multi-functional synergy. Then, low-temperature deposition 3D printing combined with thermosensitive materials is used to achieve 4D printing of the scaffold, resulting in a scaffold with a complex external morphology and a precise internal porous structure. The mechanical strength reaches the level of cortical bone, and it can actively adapt to the defect morphology after implantation, reducing intraoperative trimming time by more than 50%.

This invention belongs to the field of medical materials technology and discloses a medical composite dressing for relieving dermatitis and eczema and its preparation method. First, mussel adhesive protein is dissolved in purified water, and the pH is adjusted to obtain a mussel adhesive protein solution. Trehalose, ectoine, and a portion of glycerin are mixed and dissolved to obtain an active ingredient composite solution. Then, the mussel adhesive protein solution and the active ingredient composite solution are mixed, and the pH is adjusted to obtain phase A. Sodium carboxymethyl cellulose is dissolved in a buffer solution in batches to obtain sodium carboxymethyl cellulose solution A and sodium carboxymethyl cellulose solution B. The two solutions are mixed, and the remaining glycerin and methylparaben are added. After adjusting the pH, phase B is obtained. Finally, phase A is added dropwise to phase B, stirred, and allowed to stand for maturation to obtain the final product. The medical composite dressing provided by this invention has a unique temperature-pH response mechanism, enabling the release of active ingredients such as ectoine and trehalose under weakly acidic conditions, achieving targeted repair and moisturizing of the inflamed area.

This application relates to the development and application of a novel protein hair dye. This invention discloses a biological hair dyeing system involving three color proteins, mussel adhesive protein, and split intein, and its application, belonging to the field of biotechnology. This invention uses Escherichia coli as the chassis cell to construct and express a recombinant protein functional module mediated by split intein: three color proteins (red, yellow, and blue) connected to the C-terminus of the split intein, and a mussel adhesive protein connected to the N-terminus of the split intein. The key points of its technical solution include: (1) using red, yellow, and blue primary color proteins to achieve multi-tone and full-spectrum color coverage through precise compounding in different proportions; (2) using the excellent underwater interfacial adhesion ability of mussel adhesive protein to firmly anchor the color-developing module to the surface of hair fibers; (3) using the specific interaction between split inteins and protein trans-splicing reaction to achieve covalent connection between the color-developing module and the adhesive module at the molecular level. This invention effectively overcomes the technical bottlenecks of existing microbial pigments, such as monotonous colors and incomplete color gamut. It has the advantages of rich and controllable color development, mild hair dyeing conditions, excellent color fixing performance, and high biological safety, and has broad application prospects in the field of green cosmetics.