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Bioadhesive and preparation method thereof

A bioadhesive and solvent technology, applied in surgical adhesives, applications, medical science and other directions, can solve the problems of different components, structures and mechanical properties, and achieve good mechanical properties, broad application prospects, and good biological activity.

Active Publication Date: 2015-05-20
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these methods lead to the formation of fibrocartilage, whose composition, structure and mechanical properties are different from normal cartilage
There are currently no adhesive products available for bonding after cartilage damage

Method used

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  • Bioadhesive and preparation method thereof

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Effect test

preparation example Construction

[0024] See figure 1 , The preparation method of the bioadhesive according to the preferred embodiment of the present invention includes the following steps:

[0025] (1) Preparation of graft A, the graft A is a graft of a polymer substance and an acrylate compound.

[0026] The macromolecular substance is a natural macromolecular substance, specifically selected from at least one of hyaluronic acid, chondroitin sulfate, gelatin, polypeptide, alginic acid, and chitosan. The acrylic compound is selected from one of methyl methacrylate, methacrylate, glycidyl methacrylate, and acrylate.

[0027] This step (1) specifically includes: dissolving the polymer substance in solvent M, and then adding an acrylate compound to react, wherein the moles of the acrylic compound added are 10-50 times the moles of the polymer substance, and then The organic solvent N is added for precipitation, and the obtained precipitate is the graft A (the graft of a polymer substance and an acrylate compound).

...

Embodiment 1

[0058] Step (1): Preparation of graft A (hyaluronic acid grafted with methyl methacrylate).

[0059] 1g hyaluronic acid (molecular weight 1.6×10 6 ) Was added to 100mL phosphate buffer solution (pH=3), mechanically stirred and dissolved for 24h at a speed of 280 revolutions per minute (rpm); then 2.2mL of methyl methacrylate was added to it, and the reaction was continued for 5 days; Acetone is used for precipitation, and unreacted methyl methacrylate and other small molecules are removed. The volume of acetone added is 20 times the volume of phosphate buffer solution; after the precipitate is obtained, it is dissolved in water, and then acetone is added for precipitation. Repeat two Second; Finally, vacuum drying is carried out at room temperature for 48 hours, and the vacuum degree is -100kpa, to obtain graft A (hyaluronic acid grafted with methyl methacrylate, named hyaluronic acid acrylate).

[0060] Step (2): Oxidize the graft A.

[0061] Weigh 0.3g of graft A and add it to 5mL...

Embodiment 2

[0067] Step (1): Preparation of graft A (chondroitin sulfate grafted with methyl methacrylate).

[0068] Add 1g of chondroitin sulfate (molecular weight 40,000) to 100mL of acetone aqueous solution (the volume ratio of acetone to water is 1:1), and dissolve it under magnetic stirring for 24h at a speed of 520rpm; then add 1mL of methyl methacrylate And 1mL of triethylamine, continue to stir the reaction for 2 days; then add acetone for precipitation, and remove unreacted methyl methacrylate and other small molecules, where the volume of acetone added is 20 times the volume of the acetone aqueous solution; After dissolving the material with water, adding acetone to precipitate, repeat twice; finally, vacuum drying at room temperature for 48h, the vacuum degree is -100kpa, that is, graft A (chondroitin sulfate grafted with methyl methacrylate, named sulfuric acid) Chondroitin Acrylate).

[0069] Step (2): Oxidize the graft A.

[0070] Weigh 0.9g of graft A and add it to 5mL of aqueou...

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Abstract

The invention discloses a bioadhesive, which contains a polymer and a solvent, wherein the polymer is a graft of dopamine or mussel adhesive protein and oxidized graft A; the graft A is a graft of a macromolecular substance andan acrylate compound; a six-membered ring in a macromolecular substance in the oxidized graft A is bonded with an aldehyde group; the macromolecular substance is selected from at least one of hyaluronic acid, chondroitin sulfate, gelatin, polypeptide, alginic acid and chitosan; and the acrylate compound is selected from one of methyl methacrylate, methacrylate, glycidyl methacrylate and acrylic ester. The invention further provides a preparation method of the bioadhesive. The bioadhesive has the advantages of being good in biological activity, relatively good in mechanical property and biodegradable, and is capable of promoting tissue healing in a moist environment.

Description

Technical field [0001] The invention relates to a biological adhesive and a preparation method thereof. The adhesive is used for the adhesion and repair of tissues in a humid environment, and is particularly suitable for the adhesion and repair of cartilage tissues. Background technique [0002] In recent decades, in order to reduce the complexity of surgery and the trauma to patients, medical adhesives have been rapidly developed and widely used. In surgical operations, medical adhesives are used for local adhesion and repair of certain organs and tissues. After the operation, the sutures can be prevented from bleeding from blood vessels. Gynecology is used to block the fallopian tube to complete the ligation. Dentistry is used for tooth repair and orthopedic surgery. Combination and positioning of middle bones and joints. Currently, sutures or tissue adhesives are mainly used clinically to integrate soft tissues that need to be joined. Because tissue adhesive can effectively ...

Claims

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Application Information

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IPC IPC(8): A61L24/04A61L24/10A61L24/08
Inventor 李小丽陈昌盛王明波
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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