38 results about "Normal cartilage" patented technology

The invention discloses a constructing method of a bracket-free engineering cartilaginous tissue and a product therefrom. The constructing method comprises the following steps: inducing and differentiating human umbilical mesenchymal stem cells into cartilaginous cells and then carrying out aggregation inducing culture so that the cartilaginous cells excrete stroma towards the outside of cells and form membranoid substance; continuously inducing and culturing the membranoid substance in a cell culture box and then transferring the membranoid substance into a centrifugal tube for inducing culture to form loose cartilaginous tissue agglomerate; and continuously inducing and culturing the loose cartilaginous tissue agglomerate in a rotating wall type bioreactor to obtain dense cartilaginous-like tissue. The cytology, the histology or the immunohistochemistry dyeing shows that the constructed engineering tissue has a normal cartilaginous cell type. The constructed bracket-free engineeringcartilaginous tissue does not relate to the composite problem of the cells and an exogenous bracket, is absent from the biocompatibility problem between the cells and the bracket, is safe to human body, has even cell planting density and simple clinical application operation and can be applied to the treatment or the repair of cartilage loss.

A novel composition for regenerating a cartilage has been demanded, which can achieve a good effect of regenerating a hyaline cartilage that is a nearly normal cartilage without requiring the use of any transplanted cell. The present invention provides a composition for regenerating a cartilage, wherein (a) a monovalent metal salt of low endotoxin alginic acid and (b) SDF-1 are used in combination.

The invention discloses a patella microfracture surgery device. The patella microfracture surgery device comprises a first connecting rod, a second connecting rod and a hole puncher, wherein one end of the first connecting rod is provided with a first handle, the other end of the first connecting rod is provided with a patella bearing device attached to the upper part and / or side part of the patella, the second connecting rod is hinged to the first connecting rod, one end of the second connecting rod is provided with a second handle corresponding to the first handle, the hole puncher is arranged at the other end of the second connecting rod and comprises a hole driller and a spherical housing, the spherical housing is internally provided with a hole driller channel, and the hole driller channel is provided with an opening facing the patella bearing device and used for at least part of hole driller exposed and a punching drive mechanism for driving at least part of hole driller to be exposed out of the opening. The patella microfracture surgery device can stably and reliably fix the patella, can conveniently adjust the punching position and punching depth, can conduct microfracturepunching operation at any position of the cartilage face of the patella, is simple in operation and can avoid iatrogenic injury to the normal cartilage.

The invention discloses a tissue engineering cartilage and a preparation method thereof. The tissue engineering cartilage is cytoactive, consists of sequentially laminated cell patches including a surface layer, a middle layer, a deep layer and a calcified layer, and takes collagen matrix as a main component; the layers are compounded by collagen and hyaluronic acid, and the bordering integration of various layers of cartilage structures is promoted through hydrostatic culture; and the tissue engineering cartilage is prepared by preparing various layers of cartilage cell patches and assembling multiple structural layers. The prepared tissue engineering cartilage is in a layering arrangement of a natural cartilage, has a very good tissue structure and very good mechanical properties (frictional coefficient is 75-85 percent that of a natural cartilage tissue, and the elastic modulus is 80-90 percent that of the natural cartilage tissue.); the integration between different layers is good; experiments prove that the prepared tissue engineering cartilage can form the tidal line same as that of a normal cartilage tissue and has the same layering structure; and the prepared tissue engineering cartilage is capable of promoting regeneration and function recovery of a cartilage and obviously shortening the cycle of cartilage damage treatment and is applicable to cartilage defect restoration for a load-bearing part of a articular cartilage.

An articular cartilage graft and a preparation method thereof. The articular cartilage graft prepared by the present invention consists of three layers from the outside to the inside: superficial layer, middle layer and deep layer, and the thickness, shape and size of each layer are matched with the cartilage damage site, No need for preoperative shaping, after implantation, it corresponds to the distribution of the surrounding normal cartilage layers, which is conducive to the signal transmission, transduction and regulation between cells, and can better integrate with the surrounding normal cartilage tissue; it has a structure consistent with natural cartilage Features, from the superficial layer to the deep layer, the content of type II collagen gradually decreases, the content of GAG gradually increases, and the compression resistance and abrasion resistance are good; the chondrocytes in the cartilage graft are wrapped by extracellular matrix, and the immunogenicity is low, avoiding implantation After immune rejection occurs, it can survive and function for a long time, and improve the long-term curative effect of cartilage repair.

The invention provides a preparation method of a composite hydrogel. The preparation method comprises the following steps: S1) carrying out a reaction between chondroitin sulfate and a first acrylate monomer in a phosphate buffer solution to obtain a chondroitin sulfate acrylate hydrogel monomer; oxidizing the chondroitin sulfate acrylate hydrogel monomer to obtain an oxidized chondroitin sulfate acrylate hydrogel monomer; S2) under an ultraviolet radiation condition, carrying out a polymerization reaction between the oxidized chondroitin sulfate acrylate hydrogel monomer and a degradable hydrogel monomer through initiation of a photoinitiator so as to obtain the composite hydrogel. Compared with the prior art, a Schiff's base reaction of aldehyde groups contained in the oxidized chondroitin sulfate acrylate hydrogel monomer and amino groups existing in a tissue can be carried out, so that the adhesion of the tissue inside the body is more facilitated; moreover, the composite hydrogel contains normal cartilage tissue components, a novel cartilage tissue is easy to form quickly, and the composite hydrogel has potential application values in the aspect of clinical cartilage repair.

The invention belongs to the technical field of biomedical engineering, especially the technical field of bionic material preparation, and discloses a bionic cartilage based on 3D printing and a manufacturing method thereof, including a bionic cartilage based on 3D printing and a 3D printed bionic cartilage Manufacturing method. The 3D printing-based biomimetic cartilage is a multi-layer structure, which is prepared by gradient biomimetic 3D printing from modified type II collagen, modified hyaluronic acid, nano-hydroxyapatite and chondrocytes. The bionic cartilage has good biocompatibility and mechanical properties equivalent to normal cartilage tissue, and is beneficial to the repair of cartilage defect parts.

The invention provides a manufacturing method of a gradient tissue engineering scaffold. The manufacturing method comprises the following steps: printing a biological high molecular material and hydrogel into three types of grids by utilizing a low-temperature deposition 3D (Three Dimensional) printer, so as to obtain a hierarchical structure which comprises a tangential grid corresponding to superficial-layer tangential fibers, a uniform grid corresponding to middle-layer transition fibers and a normal grid corresponding to bottom-layer normal fibers; forming a grid bracket, wherein the grid bracket has the mechanical strength characteristics that a compression resisting force is gradually increased from shallow to deep and an anti-shearing force is gradually reduced from shallow to deep; after printing, carrying out freeze drying on the grid bracket for a first time; adding a dECM (decellularized Extracellular Matrix) solution into pores of the formed grid bracket; carrying out freeze drying for a second time and removing a solvent component in the dECM solution, so as to obtain the composite gradient tissue engineering scaffold which is close to a normal cartilage directional micro-tissue. According to the manufacturing method, one-step molding of the gradient composite scaffold can be realized and a bionic effect is remarkably optimized; quantitative mechanical strength optimization can be carried out very well.

The invention discloses a repairing tool for articular cartilage injuries. The repairing tool comprises a rod piece, the rod piece comprises a first section and a second section, the first section isused for receiving rotation driving, the end of the second section is provided with a spherical head, the repairing tool further comprises an annular elastic cover, and the end of the first section, the annular elastic cover and the end of the second section are sleeved in sequence; friction resistance between the annular elastic cover and the first section is set to be within a preset range, andwhen the resistance between the annular elastic cover and the first section is larger than the preset range, the portion between the annular elastic cover and the first section is idle. According to the repairing tool for the articular cartilage injuries, the two ends and middle of the rod piece are connected through the annular elastic cover, when the resistance is large, the annular elastic cover decouples transmission, in this way, through reasonable pressure selection, the spherical head can get rid of injured cartilages, and gets stuck when meeting normal cartilages, and thus the probability that normal cartilage tissue is gotten rid of is lowered.