38 results about "Normal cartilage" patented technology

An articular cartilage graft and a preparation method thereof are provided. The prepared articular cartilage graft is composed of a superficial layer, a middle layer and a deep layer from outside to inside; the thickness, shape and size of each layer are all matched with a cartilage injury part, and thus preoperative shaping is not required; after grafting, the articular cartilage graft has layer distribution corresponding to distribution of each layer of surrounding normal cartilages, is conducive to intercellular signal transmission, transduction and regulation, and can be better integrated with surrounding normal cartilage tissues; the articular cartilage graft has structure characteristics consistent with those of the natural cartilages, the collagen type II content is gradually decreased from the superficial layer to the deep layer, the GAG content is increased gradually from the superficial layer to the deep layer, and compression resistance and wear resistance are good; and chondrocytes in the cartilage graft are wrapped with an extracellular matrix, have low immunogenicity, allow generation of immunologic rejection to be avoided after grafting, can survive for a long term and exert functions, and improve cartilage repair long-term curative effects.

The invention belongs to the technical field of biomedical engineering, especially relates to the technical field of bionic material preparation, discloses bionic cartilage based on 3D printing and amanufacturing method thereof, and includes bionic cartilage based on 3D printing and a method for manufacturing 3D printed bionic cartilage. The bionic cartilage based on 3D printing has a multilayerstructure, modified II type collagen, modified hyaluronic acid, nanometer nano-hydroxyapatite and cartilage cells are prepared by gradient bionic 3D printing. The bionic cartilage has good biologicalcompatibility, has mechanical properties equivalent to normal cartilage tissue, and is good for repairing defect parts of cartilage.

The invention provides a manufacturing method of a gradient tissue engineering scaffold. The manufacturing method comprises the following steps: printing a biological high molecular material and hydrogel into three types of grids by utilizing a low-temperature deposition 3D (Three Dimensional) printer, so as to obtain a hierarchical structure which comprises a tangential grid corresponding to superficial-layer tangential fibers, a uniform grid corresponding to middle-layer transition fibers and a normal grid corresponding to bottom-layer normal fibers; forming a grid bracket, wherein the grid bracket has the mechanical strength characteristics that a compression resisting force is gradually increased from shallow to deep and an anti-shearing force is gradually reduced from shallow to deep; after printing, carrying out freeze drying on the grid bracket for a first time; adding a dECM (decellularized Extracellular Matrix) solution into pores of the formed grid bracket; carrying out freeze drying for a second time and removing a solvent component in the dECM solution, so as to obtain the composite gradient tissue engineering scaffold which is close to a normal cartilage directional micro-tissue. According to the manufacturing method, one-step molding of the gradient composite scaffold can be realized and a bionic effect is remarkably optimized; quantitative mechanical strength optimization can be carried out very well.

The invention belongs to the technical field of cartilage complexes, in particular to preparation of multilayer cartilage complex containing cytokine microspheres through electrospinning 3D printing,which comprises the following steps of: S1, culturing seed cells; S2, preparing a three-phase integrated scaffold; S3, planting cells. According to the method, an electrostatic spinning 3D printing technology is adopted, the fiber diameter and the printing path can be accurately controlled, the diameter of a monofilament can be as fine as 10 micrometers, corresponding loaded cytokine microspheresare mixed in a printing material to be loaded on a printed bracket, by controlling the types and contents of the cytokines, the corresponding seed cells can be induced to differentiate to the corresponding cells on the scaffold layer by layer so as to finally form a component hierarchical structure similar to a normal cartilage tissue, the complex has a layered structure similar to the normal articular cartilage tissue, and has high structural precision; and the loaded cytokine is favorable for promoting cell proliferation and differentiation, and is favorable for repairing cartilage damage.

The invention provides a pharmaceutical composition containing sodium alginate for cartilage tissue repairing. As an active ingredient of the composition, bone morphogenetic protein-4 transfected fat is derived from stem cells, sodium alginate and calcium chloride; by virtue of the composition, gaps filled between cartilage rods as well as between the cartilage rods and peripheral cartilages and subchondral bones are completely filled by regenerated tissues and mechanical properties similar to that of normal cartilage are guaranteed; full fusion of mosaic bones and cartilages with peripheral cartilages and subchondral bones is achieved, and joint function improvement and level recovery in postoperative patients are significantly improved; in addition, the invention also provides a preparation method of the pharmaceutical composition.

The invention discloses a constructing method of a bracket-free engineering cartilaginous tissue and a product therefrom. The constructing method comprises the following steps: inducing and differentiating human umbilical mesenchymal stem cells into cartilaginous cells and then carrying out aggregation inducing culture so that the cartilaginous cells excrete stroma towards the outside of cells and form membranoid substance; continuously inducing and culturing the membranoid substance in a cell culture box and then transferring the membranoid substance into a centrifugal tube for inducing culture to form loose cartilaginous tissue agglomerate; and continuously inducing and culturing the loose cartilaginous tissue agglomerate in a rotating wall type bioreactor to obtain dense cartilaginous-like tissue. The cytology, the histology or the immunohistochemistry dyeing shows that the constructed engineering tissue has a normal cartilaginous cell type. The constructed bracket-free engineering cartilaginous tissue does not relate to the composite problem of the cells and an exogenous bracket, is absent from the biocompatibility problem between the cells and the bracket, is safe to human body, has even cell planting density and simple clinical application operation and can be applied to the treatment or the repair of cartilage loss.

The invention provides a preparation method of a composite hydrogel. The preparation method comprises the following steps: S1) carrying out a reaction between chondroitin sulfate and a first acrylate monomer in a phosphate buffer solution to obtain a chondroitin sulfate acrylate hydrogel monomer; oxidizing the chondroitin sulfate acrylate hydrogel monomer to obtain an oxidized chondroitin sulfate acrylate hydrogel monomer; S2) under an ultraviolet radiation condition, carrying out a polymerization reaction between the oxidized chondroitin sulfate acrylate hydrogel monomer and a degradable hydrogel monomer through initiation of a photoinitiator so as to obtain the composite hydrogel. Compared with the prior art, a Schiff's base reaction of aldehyde groups contained in the oxidized chondroitin sulfate acrylate hydrogel monomer and amino groups existing in a tissue can be carried out, so that the adhesion of the tissue inside the body is more facilitated; moreover, the composite hydrogel contains normal cartilage tissue components, a novel cartilage tissue is easy to form quickly, and the composite hydrogel has potential application values in the aspect of clinical cartilage repair.

The invention relates to an articular cartilage repairing material based on oxidized hyaluronic acid-type II collagen and autologous concentration bone marrow nucleated cells and a preparation method.The articular cartilage repairing material is prepared from oxidized hyaluronic acid, type II collagen and autologous concentration bone marrow nucleated cells; the autologous concentration bone marrow nucleated cells in the material have potential in self-renewal and chondrogenic differentiation, participate in cartilage defect repairing, and thus realize a perfect cartilage defect repairing effect; the repaired tissue appears like hyaline cartilage and is obviously superior to the blank control group in surface flatness, integration degree with adjacent normal cartilage, content of type IIcollagen, GAG content, forms of calcified cartilage and subchondral bone and the like, and has potential in clinical transformation.

The invention relates to an anatomical composite three-dimensional scaffold tissue engineering cartilage and a preparation method thereof. According to the anatomical composite three-dimensional scaffold tissue engineering cartilage, a scaffold is made of polycaprolactone-hydroxyapatite composite material; IGF-1 PLGA microspheres are carried on the scaffold; and the adding amount of the IGF-1 PLGA microspheres is 0.1%-2% of the weigh of the scaffold. The novel three-dimensional porous scaffold prepared by the method has good biocompatibility and biodegradability and is free of cytotoxicity, can be gradually degraded along with regeneration of normal cartilage tissues after being implanted into a living body, and is finally replaced with regenerated cartilage, so that the target of thoroughly healing cartilage injuries is reached.

A novel composition for regenerating a cartilage has been demanded, which can achieve a good effect of regenerating a hyaline cartilage that is a nearly normal cartilage without requiring the use of any transplanted cell. The present invention provides a composition for regenerating a cartilage, wherein (a) a monovalent metal salt of low endotoxin alginic acid and (b) SDF-1 are used in combination.

A tissue-engineered cartilage transplant with composite structure for treating the cartilage defect and surficial depressed defect is composed of the medically acceptable solid carrier with internal cavity, and the mixture of medically acceptable colloid and normal cartilage cells through filling said mixture in said cavity of biodegradable solid carrier.

The invention discloses a repairing tool for articular cartilage injuries. The repairing tool comprises a rod piece, the rod piece comprises a first section and a second section, the first section isused for receiving rotation driving, the end of the second section is provided with a spherical head, the repairing tool further comprises an annular elastic cover, and the end of the first section, the annular elastic cover and the end of the second section are sleeved in sequence; friction resistance between the annular elastic cover and the first section is set to be within a preset range, andwhen the resistance between the annular elastic cover and the first section is larger than the preset range, the portion between the annular elastic cover and the first section is idle. According to the repairing tool for the articular cartilage injuries, the two ends and middle of the rod piece are connected through the annular elastic cover, when the resistance is large, the annular elastic cover decouples transmission, in this way, through reasonable pressure selection, the spherical head can get rid of injured cartilages, and gets stuck when meeting normal cartilages, and thus the probability that normal cartilage tissue is gotten rid of is lowered.

The invention provides a medicinal composition comprising psoralen as an active component, and the application of the psoralen to preparation of the medicinal composition for treating osteoarthritis.The medicinal composition comprises a therapeutically effective amount of psoralen and a pharmacologically acceptable excipient or carrier. The medicinal composition comprising the psoralen can promote secretion of a cartilage matrix, protect chondrocytes and maintain a normal cartilage tissue structure, so that the medicinal composition can effectively treat the osteoarthritis.

The invention relates to a method of treating a cartilage matrix-forming bone tumour and/or a metastatic cancer originating from a cartilage matrix-forming bone tumour, for example chondrosarcoma, in which one or more of an inhibitor of RUNX2 activity, an inhibitor of RUNX2 expression, an inhibitor of YBX1 activity and an inhibitor of YBX1 expression, is administered to a subject in need thereof. The invention also relates to an in vitro method for detecting the presence of a cartilage matrix-forming bone tumour in a subject or the risk of a subject developing a cartilage matrix-forming bone tumour, for example chondrosarcoma, in which the following steps are performed: (i) measuring the expression level of at least one of RUNX2 and YBX1 in a biological sample obtained from a subject, and (ii) comparing the expression level of RUNX2 and/or YBX1 in the biological sample obtained from the subject with the respective expression level of RUNX2 and/or YBX1 in normal cartilage or other biological material. A higher expression level of RUNX2 and/or YBX1 in the biological sample obtained from the subject compared to the respective expression level of RUNX2 and/or YBX1 in the normal cartilage or other biological material indicates the presence of or an increased risk of developing a cartilage matrix-forming bone tumour.