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Recombinant lentivirus for carrying out targeted induction on osteogenic differentiated cell apoptosis as well as preparation method and application thereof

A technology of osteogenic differentiation cells and recombinant lentivirus, applied in biochemical equipment and methods, methods based on microorganisms, bone diseases, etc., can solve the problems of limited repair ability of cartilage, lack of treatment measures, etc., and achieve low cost and high preparation method easy effect

Inactive Publication Date: 2010-07-28
ARMY MEDICAL UNIV
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Problems solved by technology

[0002] Cartilage damage or loss is a common clinical problem. Due to the histological and biological characteristics of cartilage limit its own repair ability, there is still a lack of effective treatment measures

Method used

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  • Recombinant lentivirus for carrying out targeted induction on osteogenic differentiated cell apoptosis as well as preparation method and application thereof
  • Recombinant lentivirus for carrying out targeted induction on osteogenic differentiated cell apoptosis as well as preparation method and application thereof
  • Recombinant lentivirus for carrying out targeted induction on osteogenic differentiated cell apoptosis as well as preparation method and application thereof

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Embodiment Construction

[0035] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer. Unless otherwise stated, various restriction endonucleases, DNA polymerases, DNA ligases and kits in the preferred examples were purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd. and Beijing Biotech Biogene Technology Co., Ltd. company.

[0036] 1. Preparation of Osxp-Apoptin recombinant lentivirus

[0037] The schematic diagram of the construction of Osxp-Apoptin recombinant lentivirus is as follows figure 1 Shown, its preparation method comprises the following steps:

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Abstract

The invention discloses recombinant lentivirus for carrying out targeted induction on the osteogenic differentiated cell apoptosis as well as a preparation method and application thereof. A recombinant lentivirus genome contains an Apoptin gene expression box which contains an Osterix gene promoter, an Apoptin gene and a terminator, and the Apoptin gene expression is regulated and controlled by the Osterix gene promoter; the recombinant lentivirus takes a pan-osteogenic cell peculiar expression gene Osterix promoter as a promoting element, expresses Apoptin for inducing the osteogenic differentiated cell apoptosis in osteogenic differentiated cells, does not play a role on chondrogenic differentiated cells and normal cartilage cells, can be prepared into medicines for inducing the osteogenic differentiated cell apoptosis, carries out the treatment on residual osteogenic differentiated cells in seed cells after the osteogenic induced differentiation treatment and ensures a single chondrogenic differentiation path of the seed cells, thereby providing a favorable seed cell for the establishment of tissue engineering cartilage; and the preparation method of the recombinant lentivirus is simple, convenient, rapid and low in cost.

Description

technical field [0001] The present invention relates to a recombinant virus, in particular to a recombinant lentivirus targeted to induce apoptosis of osteogenic differentiation cells, and also to a preparation method and application of the recombinant lentivirus. Background technique [0002] Cartilage damage or loss is a common clinical problem. Due to the histological and biological characteristics of cartilage limit its own repair ability, there is still a lack of effective treatment measures. The tissue engineering method provides a brand-new method for the repair of cartilage defects, using scaffold materials to provide a spatial framework for the proliferation of seed cells and matrix secretion, forming a structure similar to normal cartilage tissue, and performing functions. Seed cells include embryonic stem cells and various sources of mesenchymal stem cells such as bone marrow mesenchymal stem cells, peripheral blood mesenchymal stem cells, umbilical cord blood mes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/85A61K35/76A61P19/08C12R1/93
Inventor 董世武邓均邹仲敏谢肈杨波应大君
Owner ARMY MEDICAL UNIV
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