Recombinant lentivirus for carrying out targeted induction on osteogenic differentiated cell apoptosis as well as preparation method and application thereof

A technology of osteogenic differentiation cells and recombinant lentivirus, applied in biochemical equipment and methods, methods based on microorganisms, bone diseases, etc., can solve the problems of limited repair ability of cartilage, lack of treatment measures, etc., and achieve low cost and high preparation method easy effect
CN101787359AInactive Publication Date: 2010-07-28ARMY MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ARMY MEDICAL UNIV
Publication Date
2010-07-28
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses recombinant lentivirus for carrying out targeted induction on the osteogenic differentiated cell apoptosis as well as a preparation method and application thereof. A recombinant lentivirus genome contains an Apoptin gene expression box which contains an Osterix gene promoter, an Apoptin gene and a terminator, and the Apoptin gene expression is regulated and controlled by the Osterix gene promoter; the recombinant lentivirus takes a pan-osteogenic cell peculiar expression gene Osterix promoter as a promoting element, expresses Apoptin for inducing the osteogenic differentiated cell apoptosis in osteogenic differentiated cells, does not play a role on chondrogenic differentiated cells and normal cartilage cells, can be prepared into medicines for inducing the osteogenic differentiated cell apoptosis, carries out the treatment on residual osteogenic differentiated cells in seed cells after the osteogenic induced differentiation treatment and ensures a single chondrogenic differentiation path of the seed cells, thereby providing a favorable seed cell for the establishment of tissue engineering cartilage; and the preparation method of the recombinant lentivirus is simple, convenient, rapid and low in cost.
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Description

technical field

[0001] The present invention relates to a recombinant virus, in particular to a recombinant lentivirus targeted to induce apoptosis of osteogenic differentiation cells, and also to a preparation method and application of the recombinant lentivirus. Background technique

[0002] Cartilage damage or loss is a common clinical problem. Due to the histological and biological characteristics of cartilage limit its own repair ability, there is still a lack of effective treatment measures. The tissue engineering method provides a brand-new method for the repair of cartilage defects, using scaffold materials to provide a spatial framework for the proliferation of seed cells and matrix secretion, forming a structure similar to normal cartilage tissue, and performing functions. Seed cells include embryonic stem cells and various sources of mesenchymal stem cells such as bone marrow mesenchymal stem cells, peripheral blood mesenchymal stem cells, umbilical cord blood mes...

Claims

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