A kind of preparation method of gradient tissue engineering scaffold

Abstract

The invention provides a manufacturing method of a gradient tissue engineering scaffold. The manufacturing method comprises the following steps: printing a biological high molecular material and hydrogel into three types of grids by utilizing a low-temperature deposition 3D (Three Dimensional) printer, so as to obtain a hierarchical structure which comprises a tangential grid corresponding to superficial-layer tangential fibers, a uniform grid corresponding to middle-layer transition fibers and a normal grid corresponding to bottom-layer normal fibers; forming a grid bracket, wherein the grid bracket has the mechanical strength characteristics that a compression resisting force is gradually increased from shallow to deep and an anti-shearing force is gradually reduced from shallow to deep; after printing, carrying out freeze drying on the grid bracket for a first time; adding a dECM (decellularized Extracellular Matrix) solution into pores of the formed grid bracket; carrying out freeze drying for a second time and removing a solvent component in the dECM solution, so as to obtain the composite gradient tissue engineering scaffold which is close to a normal cartilage directional micro-tissue. According to the manufacturing method, one-step molding of the gradient composite scaffold can be realized and a bionic effect is remarkably optimized; quantitative mechanical strength optimization can be carried out very well.

Description

technical field [0001] The invention relates to the field of biological tissue engineering, in particular to a preparation method of a gradient tissue engineering scaffold. Background technique [0002] Tissue engineering is an emerging discipline that combines cell biology and material science to construct tissues or organs in vitro or in vivo. Through the mixed culture of cells, scaffold structures and growth factors, the graft materials suitable for repairing human tissue or organ defects can be manufactured, and the structure regeneration and functional reconstruction of the defect site can be realized. The tissue engineering technology used in the human body puts forward higher requirements on the biological and physical and chemical properties of materials, among which the reconstruction of bone and cartilage tissue has the highest requirements on mechanical strength, and also puts forward certain requirements on microscopic tissue morphology. [0003] Bone and cartil...

AI Technical Summary

Problems solved by technology

However, the common cartilage scaffolds are mostly homogeneous or quasi-homogeneous structures, and the mechanical strength is not biomimetic enough.

Many papers and patents CN201410097482 have introduced cartilage scaffolds with gradient structures, but their production methods are mostly layered bonding, layered casting or multi-material printing, which has cumbersome processes, poor bonding / casting interface strength, and difficult Quantitative mechanical strength optimization and other issues

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