The application belongs to the technical field of
cell culture, and discloses a
T cell activation and expansion method based on self-adaptive liquid-
liquid interface and application thereof. The application provides application of a liquid-
liquid interface culture
system in
T cell activation and expansion, wherein the liquid-
liquid interface comprises a two-dimensional liquid-liquid interface and a liquid-liquid interface
microsphere, both of which can form a
protein nanofilm at an oil-water interface, and the
protein nanofilm is used to load molecules providing
T cell receptor activation signals and / or molecules providing T
cell costimulatory signals, so as to construct a self-adaptive T
cell activation interface with dynamic molecular display capability. Compared with a traditional rigid
solid culture dish and a
magnetic bead system, the liquid-liquid interface has fluidity, molecular migration and interface responsiveness, and is more conducive to T cell
receptor aggregation, formation of an immunological
synapse-like structure and synergistic integration of T cell
receptor activation signals and costimulatory signals.