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Tissue engineering fiber bundle structure body and preparation method thereof

A technology of fiber filaments and hydrogel fibers, applied in the biological field, can solve the problems of complex forming process and difficult control of internal structure

Active Publication Date: 2012-06-20
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Assembly (Assembly) is currently divided into two types: random stacking and orderly arrangement. The former has a simple process, but the internal structure is difficult to control; although the latter can form a regular structure, the forming process is very complicated and still needs further work. explore

Method used

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  • Tissue engineering fiber bundle structure body and preparation method thereof
  • Tissue engineering fiber bundle structure body and preparation method thereof
  • Tissue engineering fiber bundle structure body and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Embodiment 1, matrix material is the forming construction of the hydrogel fiber silk of sodium alginate / cell culture fluid

[0110] 1. The formation and construction of hydrogel fiber filaments with sodium alginate / cell culture medium as the matrix material

[0111] 1) Solution preparation

[0112] Prepare a 0.05 g / ml sodium alginate aqueous solution, place it at 60° C. to dissolve it completely, and perform sterilization treatment.

[0113] Prepare 0.05g / ml calcium chloride aqueous solution and sterilize it.

[0114] Preparation of cell culture fluid A: It is obtained by mixing fetal bovine serum, penicillin, streptomycin and high-sugar DEME culture fluid, the concentration of said fetal bovine serum in said cell culture fluid is 10% (volume percentage), so The concentration of said penicillin and streptomycin in said cell culture medium is 100U / ml.

[0115] Preparation of cell culture medium B: high-sugar DEME medium, Invitrogen Cat. No. 12800-017), placed at 4°C f...

Embodiment 2

[0124] Example 2. Forming and construction of sodium alginate hydrogel fiber filaments containing cardiomyocytes and wrapping endothelial cells

[0125] 1. Formation and construction of sodium alginate hydrogel fibers containing cardiomyocytes and wrapping endothelial cells

[0126] 1) Solution preparation

[0127] Prepare 2.5% sodium alginate aqueous solution and 0.05 g / ml calcium chloride aqueous solution in the same manner as in Example 1.

[0128] Preparation of cardiomyocyte suspension, isolated cardiomyocytes were extracted from sacrificed neonatal suckling mice (purchased from the Experimental Animal Center of Peking University Health Science Center, Sprague-Dawley rats), cultured in cell culture solution A, and placed in a 37°C carbon dioxide incubator for standby . 75cm of cardiomyocytes will be overgrown when used 2 Aspirate the cell culture solution in the cell culture bottle, add 2ml of cell digestion solution, shake gently to make it cover the bottom of the bot...

Embodiment 3

[0150] Embodiment 3, matrix material is the shaping construction of the hydrogel fiber silk of sodium alginate / fibrin

[0151] 1. Forming and construction of hydrogel fiber filaments with sodium alginate / fibrin as the matrix material

[0152] 1) Solution preparation

[0153] Prepare 5% (0.05g / ml) sodium alginate aqueous solution and 0.05g / ml calcium chloride aqueous solution with embodiment 1.

[0154] Preparation of 5% fibrinogen solution: fibrinogen (purchased from Sigma, product number F8630) was dissolved in DEME high-glucose culture medium, and placed at 37° C. to dissolve completely.

[0155] Preparation of 30 U / ml thrombin solution: prepare a thrombin solution (purchased from Sigma, product number T4648) with DEME high-sugar culture medium at a concentration of 30 U / ml, and place it at 4°C for use.

[0156] Preparation of cell culture fluid A: It is obtained by mixing fetal bovine serum, penicillin, streptomycin and high-sugar DEME culture fluid, the concentration of ...

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Abstract

The invention discloses a tissue engineering fiber bundle structure body and a preparation method thereof. The invention provides a method A or B for preparing hydrogel fiber filaments containing cellular components. The method A comprises the following steps: 1) compositing a hydrogel material utilized as a matrix with cellular components to obtain a mixture containing cellular components; and 2) putting the mixture obtained in the step 1) into crosslinked fluid corresponding to the matrix to undergo crosslinking, thus obtaining the hydrogel fiber filaments containing cellular components. Experiments prove that the design can comprehensively meet the special bionic requirements of high-density cell composition, good vascularization development trend and achievement of directionally ordered structure in tissue engineering, so that the structure body is good in vascularization development prospect and can hopefully break through the development bottleneck that the tissue engineering can not become large and thick at present.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tissue engineering fiber bundle structure and a preparation method thereof. Background technique [0002] "Tissue Engineering (Tissue Engineering)" (Langer R, VacantiJ P. Tissue engineering. Science, 1993, 260(5110): 920-926.), proposed in the 1980s, aims to construct three-dimensional structures of cells and materials in vitro , through proper cultivation and training, it develops into a transplantable substitute with a structure and primary function similar to natural tissue. In recent years, tissue engineering has been a research hotspot at home and abroad, with rapid development and many achievements. Its main products are concentrated in a few fields such as bone tissue engineering and skin tissue engineering. The current in vitro construction of tissue engineering structures has involved a variety of methods, such as hydrogel casting, electrospinning, cell printing, three-di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F9/04D01F1/10D01F11/00D01F8/18D01F8/02D01F11/02D06M16/00D06M15/15C12S11/00D01D5/06A61L27/20A61L27/22A61L27/24A61L27/36C12N11/10C12Q1/02D06M101/04D06M101/10D06M101/14
Inventor 熊卓黎铭峰张婷孙伟张人佶林峰
Owner TSINGHUA UNIV
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