Method for preparing hBMSCs/hyaline cartilage particle/calcium alginate gel compound and application thereof

Abstract

The invention provides a preparation method of hBMSCs / hyaline cartilage particles / calcium alginate gel complex, the preparation method uses 0.125mm 3 Hyaline cartilage granules as small as 100000000000000000 hBMSCs are induced and cultured, and hBMSCs / hyaline cartilage granules / calcium alginate gel complex is prepared to repair the defect on the cartilage surface, which can simultaneously rebuild the strong subchondral bone support and restore the integrity The smooth articular cartilage surface can fill the subchondral trabecular space and form a seamless connection with the adjacent defect. The presence of hyaline cartilage particles can form a part of extracellular matrix simulating normal cartilage and part of normal chondrocytes in the repair tissue, which is conducive to accelerating the reconstruction process and enhancing the strength of the repair tissue on the surface. The hBMSCs used can play a dual role , can delay the wear of the articular surface and the degeneration of the adjacent articular surface.

Description

technical field [0001] The invention relates to a preparation method and application of hBMSCs / hyaline cartilage particles / calcium alginate gel complex. Background technique [0002] Intra-articular osteochondral defect and injury are common clinical diseases and are difficult to treat. Osteochondral compound defect refers to a compound defect that includes articular cartilage tissue defect and subchondral bone defect at the same time. Hyaline cartilage tissue lacks self-repair ability, which will lead to Outcomes in osteoarthritis. At present, the commonly used treatment methods include subchondral bone drilling, microfracture, autologous or allogeneic cartilage transplantation, chondrocyte transplantation, and tissue engineering cartilage tissue transplantation. All have limited repair ability, repair tissue is composed of fibrocartilage, cannot repair subchondral bone defects at the same time, lack of mechanical strength of repair tissue, disease spread, limited donor ar...

AI Technical Summary

Problems solved by technology

All have limited repair ability, the repair tissue is composed of fibrocartilage, cannot repair subchondral bone defects at the same time, the mechanical strength of the repair tissue is insufficient, the disease spreads, the donor area is limited, the steps are cumbersome, and the cost is expensive, etc., and there are many complications.

At present, there is no ideal method that can satisfy both subchondral bone support and smooth cartilage surface at home and abroad.