Method for preparing hBMSCs/hyaline cartilage particle/calcium alginate gel compound and application thereof

Abstract

The invention provides a method for preparing an hBMSCs / hyaline cartilage particle / calcium alginate gel compound. According to the method, the hBMSCs / hyaline cartilage particle / calcium alginate gel compound is prepared by using hyaline cartilage particles having a size of 0.125mm<3> after inducing culture is carried out on hBMSCs and is used for repairing defects on the cartilage surface, a strong subchondral bone support can be reconstructed, a complete and smooth articular cartilage surface can be recovered, the trabecular space of the subchondral bone can be filled, and seamless connection can be formed with adjacent defects. Due to the existence of the hyaline cartilage particles, partial normal-simulated cartilage extracellular matrix and partial normal cartilage cells can be formed in the repaired tissue, so that the reconstruction process can be accelerated, the repaired tissue strength on the surface can be enhanced, double effects can be achieved by adopting hBMSCs, and abrasion of the joint surface and regression near the joint surface can be retarded.

Description

technical field

[0001] The invention relates to a preparation method and application of hBMSCs / hyaline cartilage particles / calcium alginate gel complex. Background technique

[0002] Intra-articular osteochondral defect and injury are common clinical diseases and are difficult to treat. Osteochondral compound defect refers to a compound defect that includes articular cartilage tissue defect and subchondral bone defect at the same time. Hyaline cartilage tissue lacks self-repair ability, which will lead to Outcomes in osteoarthritis. At present, the commonly used treatment methods include subchondral bone drilling, microfracture, autologous or allogeneic cartilage transplantation, chondrocyte transplantation, and tissue engineering cartilage tissue transplantation. All have limited repair ability, repair tissue is composed of fibrocartilage, cannot repair subchondral bone defects at the same time, lack of mechanical strength of repair tissue, disease spread, limited donor ar...