The invention discloses a puncture bag for cartilage destruction and sampling of rabbit knee joints. The puncture bag comprises a puncture sleeve, a puncture needle and an injector, wherein the puncture needle can be inserted into the puncture sleeve, the needle tail end of the puncture needle is fixedly sleeved with a puncture needle handle, the needle head end of the puncture needle extends intothe sleeve and penetrates through the sleeve, the needle tail of the puncture needle is connected with a needle plug, a fixing block is fixed to the needle head end, penetrating out of the puncture sleeve, of the puncture needle, a plurality of rotating shafts are fixed to the outer circumferential wall of the fixing block, cartilage surface destroying plates are rotatably connected to the rotating shafts, and a plurality of cartilage surface destroying nail thorns are fixedly connected to the cartilage surface destroying plates. The puncture bag has the beneficial effects that the cartilagesurfaces of the knee joints can be destroyed by opening the umbrella-shaped cartilage surface destroying plates of the cartilage surface destroying nail thorns, the knee joint tissue destroying and sampling efficiency is improved, the minimally invasive operation ensures that the pollution is avoided, meanwhile, the damage area and the material taking size are controllable, and the scientificity and standardization are improved.