15 results about "Fibrin adhesive" patented technology

The invention discloses a preparation method and application of autologous fibrin glue, and belongs to the field of biomedical materials. The preparation method comprises the following five steps: collecting autologous venous blood, adding a sodium citrate anticoagulant with the volume ratio of 1: (8-12), and storing at 4-6 DEG C for not more than 2 hours; performing step-by-step centrifugation at the speed of 1500 to 2000 r / min and the speed of 3500 to 4000 r / min to obtain a fibrinogen concentrate; the thrombin-calcium chloride activation solution A and the tranexamic acid activation solution B are mixed according to the ratio of 1: (2-3) to form a composite activation system; mixing the two components according to a mass ratio of 1: (0.8-1.2), and reacting at 25-37 DEG C for 3-5 minutes to form colloid; and pre-freezing at-40 to-60 DEG C, performing vacuum freeze-drying, and storing at 4-8 DEG C. During application, the colloid can be coated on an operation wound surface after being redissolved, or mixed with a growth factor to prepare a dressing for covering a chronic wound surface. The colloid is high in purity, stable in coagulation time, long in storage period, suitable for scenes such as surgical hemostasis and the like, and high in clinical value.

The invention discloses a hydrogel adhesive based on gelatin and a clay material and a preparation method of the hydrogel adhesive. The hydrogel adhesive at least comprises the following preparation raw materials: modified gelatin, a modified clay material and a thiolated cross-linking agent. The hydrogel adhesive prepared by the invention has an excellent tissue adhesion effect in a moist environment, can form gel within 30-60s, has flexibly adjustable adhesion performance, and can meet the application of various surgical scenes. The fibrin glue has the blood coagulation promoting effect, the hemostasis effect is better than that of traditional fibrin glue, the antibacterial effect is achieved, the risk of wound infection is reduced, meanwhile, good elasticity and toughness are achieved, the matching degree with tissue mechanical properties is high, and the fibrin glue cannot lose efficacy during cyclic compression. The biological compatibility is good, the anti-inflammatory and anti-oxidation effects are achieved, and wound healing and regeneration can be accelerated.

The application belongs to the technical field of biological medicine, and particularly relates to the use of a lung extracellular matrix hydrogel in the preparation of a lung injury repair material. The application grafts methacrylic anhydride groups on a lung tissue-derived extracellular matrix, and provides a lung extracellular matrix hydrogel which can be rapidly photo-crosslinked, has high stability, effectively promotes angiogenesis, and prevents postoperative leakage. The photo-crosslinked lung extracellular matrix hydrogel of the application is applied to a lung injury animal model, significantly promotes angiogenesis, promotes alveolar regeneration, and also significantly reduces the fibrosis level. Compared with a sealing agent alpha-cyanopropyl acrylate gel and a fibrin glue which are used in clinical application, the photo-crosslinked lung extracellular matrix hydrogel has excellent effects on repairing lung injury, provides a new strategy for repairing traumatic and iatrogenic lung injury, and has a good application prospect.

The invention relates to the technical field of atomization drug delivery devices, and discloses a fibrin adhesive atomization drug delivery device which comprises a device bottom table, a drug bin, an atomization piece, an air outlet pipe, a flow dividing mechanism, a covering cover and a clamping mechanism, the drug bin is arranged at the top of the device bottom table and used for containing a fibrin adhesive, and a bin cover is arranged at the top of the drug bin and used for containing the fibrin adhesive. The atomizing piece is used for atomizing the fibrin adhesive and fixedly arranged in the device bottom table, the air outlet pipe is used for discharging atomized medicine and communicates with the medicine bin through the atomizing piece, and the flow dividing mechanism is used for dividing atomized steam flow. According to the fibrin adhesive atomization administration device, the shunting mechanism is arranged, and multidirectional pipelines of a three-directional shunting pipe are used for shunting, so that the administration device can carry out multidirectional administration at one time, and can carry out atomization administration treatment on a plurality of wound surfaces at the same time; the convenience and the treatment efficiency of multi-wound atomization hemostasis treatment are improved.

The invention relates to the technical field of developing gold labels, and discloses a near-tumor developing liquid gold label and a using method and application thereof.The near-tumor developing liquid gold label comprises a mixed solution composed of iodine oil and biological hemostatic glue, the iodine content of the iodine oil is not smaller than 40%, and the biological hemostatic glue is selected from fibrin glue and gelatin-based hemostatic glue. The mass ratio of the iodine oil to the biological hemostatic glue is 1: 1-1: 2; the liquid gold label provided by the invention has remarkable economical efficiency, and the production and implantation cost of the gold label is reduced, so that the liquid gold label is widely applied in clinic; meanwhile, due to the adoption of a non-metal material, the risk of in-vivo implantation is reduced, the occurrence rate of complications such as infection and bleeding is reduced, and the safety and acceptance of a patient are improved; besides, the liquid gold label does not generate artifacts in imaging examination, so that accurate judgment of a doctor on the position and the form of the tumor is ensured, and the accuracy and the safety of SBRT are improved.

The invention belongs to the technical field of biomedicine, and particularly relates to application of lung extracellular matrix hydrogel in preparation of a lung injury repair material. A methacrylic anhydride group is grafted on a lung tissue-derived extracellular matrix, and the lung extracellular matrix hydrogel capable of being quickly photo-crosslinked is provided, is high in stability, effectively promotes angiogenesis and prevents postoperative leakage. When the photo-crosslinking lung extracellular matrix hydrogel is applied to a lung injury animal model, the generation of blood vessels is remarkably promoted, the regeneration of pulmonary alveoli is promoted, and the fibrosis level is also remarkably reduced. Compared with clinically used sealants alpha-cyanoacrylate gel and fibrin glue, the sealant has an excellent lung injury repairing effect, provides a new strategy for trauma and iatrogenic lung injury repairing, and has a good application prospect.

The present invention relates to a composition for transplantation comprising an organoid, and a use of same. According to one example, using collagen, gelatin or fibrin glue as a scaffold for organoid transplantation results in a high transplantation rate and a high survival rate of organoid as well as desirable stability.

The present invention relates to a method for constructing a mouse tumor model using a single tumor cell. In particular, the method according to the present invention comprises the step of transplanting a fibrin gel comprising a single tumor cell into a mouse; preferably, the method comprises the step of transplanting a fibrin gel comprising a single tumor cell into the subcutis of a mouse.

The application belongs to the technical field of biological medicine, and discloses application of antibacterial peptide humAMP_23 in preparation of a medicine for treating burn infection, and is used for promoting healing of a burn wound surface. It is found that local application of the antibacterial peptide humAMP_23 can effectively reduce inflammatory reaction and mortality. It is proved by cell experiments that humAMP_23 has no cytotoxicity and good biocompatibility. The application further provides a pharmaceutical composition containing the antibacterial peptide humAMP_23 and a pharmaceutically acceptable local external carrier. The pharmaceutical composition can be prepared into various forms such as gels, creams, ointments, sprays, hydrocolloid dressings or fibrin glue, is convenient to use in medicine, has the advantages of convenient use, high local drug concentration, remarkable effect of promoting wound healing, and is suitable for local administration of burn infection wound, especially burn infection wound caused by Staphylococcus aureus or Pseudomonas aeruginosa.

To provide a lesion site identification method capable of safely identifying a lesion site of an organ by suppressing exposure to radiation and pain of a patient.SOLUTION: A lesion site identification method includes the steps of: creating, on the basis of data obtained by lung CT scan, a life-size pulmonary lobe model of a pulmonary lobe including a lesion site by a 3D printer, the pulmonary lobe model representing a position corresponding to the lesion site on an outer shell thereof; covering the pulmonary lobe model with a PGA sheet, transferring a shape of the pulmonary lobe model onto the PGA sheet, and creating a pleural sheet by fixing it with fibrin glue; marking the pleural sheet at a position corresponding to the lesion site of the pulmonary lobe; covering the pulmonary lobe with the marked pleural sheet; and identifying the lesion site on the basis of the mark on the pleural sheet covering the pulmonary lobe.SELECTED DRAWING: Figure 1

The invention provides a medicine composition for treating cancer and a preparation method, a using method and application thereof, the medicine composition comprises PROTAC and fibrin glue loaded with CAR-T cells, on one hand, the PROTAC is high in oral bioavailability, oral administration is facilitated, and the medicine dosage can be adjusted at any time; on the other hand, the utilization rate of the CAR-T cells can be increased through a method for in-situ delivery of the CAR-T cells after operation; when the PROTAC and the HPK1 are combined for application, the obvious synergistic interaction is realized, the PROTAC can realize targeted HPK1 degradation, enhance the activity of CAR-T cells and the tumor infiltration capability, obviously improve the killing capability of the CAR-T cells on tumor cells and inhibit solid tumor recurrence, so that the anti-tumor effect of the CAR-T cells is obviously promoted, and the PROTAC has a good application prospect.

An embodiment of the present disclosure provides an extracellular matrix-based bioadhesive as an adhesive in the form of a composition including an extracellular matrix-containing hydrogel and a gelatin curing agent, wherein the extracellular matrix-containing hydrogel is gelatinized. Since the extracellular matrix-based bioadhesive according to an embodiment of the present disclosure has the same or similar rheological properties as gelatin, the bioadhesive has flowability at a temperature of 30° C. or higher and may be evenly and easily applied to a lesion site in the body. In addition, the extracellular matrix-based bioadhesive according to an embodiment of the present disclosure may adhere well to the lesion site because of a level of adhesive strength that is about 2 to 6 times higher than that of fibrin glue used as a commercial tissue adhesive. In addition, the extracellular matrix-based bioadhesive according to an embodiment of the present disclosure is based on a tissue-derived extracellular matrix, and thus includes a tissue-derived wound healing component or a tissue regeneration component, and may be used for wound healing or tissue regeneration in addition to bioadhesive applications.

The invention provides a collagen self-adhesive cartilage repair scaffold as well as a preparation method and application thereof, and relates to the technical field of biomedical materials, the collagen self-adhesive cartilage repair scaffold sequentially comprises a collagen compact layer, a collagen loose layer and a biological adhesive layer covalently crosslinked with the collagen loose layer; the biological bonding layer comprises a multi-arm PEG derivative, and a terminal functional group of the multi-arm PEG derivative is NHS ester. The NHS ester can be subjected to a covalent reaction with amino groups of collagen in cartilage tissue, joint fluid scouring and joint activity shearing force are resisted, the initial adhesion strength and stability of the cartilage repair scaffold in a wet dynamic environment are improved, the postoperative early displacement or falling risk is reduced, and persistent repair of cartilage defects is promoted. The technical problems that in the prior art, due to the fact that a cartilage repair support depends on suture or fibrin glue fixing, surgical operation is complex, initial mechanical stability is insufficient, and the integration failure rate is high are solved.

The invention relates to the technical field of cartilage repair, in particular to a preparation method and application of injectable collagen gel. The method comprises the following specific steps: (1) carrying out low-temperature pretreatment and irradiation sterilization on a collagen solution; (2) mixing the physiological pH buffer solution B with moisturizing, lubricating and repairing components and a composite nutrient solution, and adjusting the pH value to 7.5-8.5 to obtain a neutralization solution A; and (3) mixing and neutralizing the collagen solution and the neutralization solution A to complete collagen self-assembly to form the hydrogel. The physicochemical property and the structure of the gel are more suitable for adhesion, growth, proliferation and differentiation of cartilage cells, and are more suitable for tissue engineering cartilage research. The technology does not need to be combined with a micro-fracture surgery, does not need to be bonded by virtue of fibrin glue, can be directly operated in joint fluid, does not need a dry environment, does not damage subchondral bones, protects the joint structure of a patient, increases the proliferation migration rate of cells, greatly reduces the operation cost, and simultaneously reduces the technical threshold of an operation hospital and an operation doctor.

The utility model provides an exosome fibrin adhesive preparation device which comprises a preparation tank, a first material storage assembly, a second material storage assembly and a conveying assembly, the left side surface of the preparation tank is provided with the first material storage assembly, and the upper side surface of the preparation tank is provided with the second material storage assembly. Compared with the prior art, the device has the advantages that the first storage assembly and the second storage assembly are arranged to store different raw materials respectively, raw material management is more orderly, meanwhile, exosome, fibrous protein and other raw materials can be conveniently added through the discharging structures of the first storage assembly and the second storage assembly, operation efficiency is improved, the conveying assembly is arranged, and the conveying efficiency is improved. In the using process, the auger piece can stably control the discharging amount, it is ensured that the amount of adhesive output every time is stable, the conveying motor drives the auger piece to achieve rapid and continuous material conveying, and the production efficiency is improved.