54 results about "Fresh frozen" patented technology

The articular cartilage according to the invention is made of pure cartilage and is provided with incisions (12) on the surface facing the bone. The cartilage cells are preferably seeded on the surface provided with incisions (12). The method for producing the articular cartilage comprises the step of collecting cartilage from joints, wherein pure cartilage is collected without bone, and incisions are made on the surface of the cartilage intended to face the bone. It is preferably fresh frozen until use. The device for harvesting articular cartilage, comprises handle and cutting blade, wherein the cutting blade (4) is curvilinear and is provided with spacer elements (5), meanwhile the device for producing incisions in articular cartilages comprises handle (2) and a bridge (3) connected to said handle (2) and being provided with one or more cutting blade(s) (4). During the method for applying the articular cartilage the articular cartilage is fixed by thin surgical yarn stitches, by fibrin glue or by small anchors (FIG. 8).

The invention discloses a fresh frozen shrimp antifreeze agent. A water solution system of the fresh frozen shrimp antifreeze agent is characterized by comprising the following components in percentage by weight: 0.09%-0.28% of low-acyl gellan gum, 1.5%-2.5% of trehalose, 1.0%-3.0% of sodium lactate, 0.8%-1.6% of sodium citrate and 0.2%-0.8% of sodium ascorbate, wherein the molecular weight of the low-acyl gellan gum is 0.5*10<5>Da to 1*10<5>Da. Compared with a traditional phosphate antifreeze agent, the antifreeze agent disclosed by the invention is relatively safe and efficient to use; the freezing denaturation of shrimp protein and color deepening in the frozen storage process can be effectively weakened; the moisture and the original flavor of the shrimp are kept; and compared with a non-phosphorus antifreeze agent, the antifreeze agent disclosed by the invention is relatively low in sweetness and heat, conforms to the consumption trends of low sweetness and low heat, can be applied to a long-term storage process of the frozen shrimp instead of the phosphate antifreeze agent, and can also be applied to frozen storage of other aquatic products.

The invention discloses edible composite sodium alginate antibacterial film liquid as well as a preparation method and application thereof. The edible composite sodium alginate antibacterial film liquid is characterized in that by taking a sodium alginate solution with the mass concentration of 1%-4% as a film base solution, glycerin accounting for 0.5%-1.5% of the film base solution by mass, epsilon-polylysine accounting for 0.15% -0.5% of the film base solution by mass and shaddock peel nanofiber accounting for 0.15%-0.25% of the film base solution by mass are added, citric acid is added to adjust the pH value of the solution to 5-6 and then uniform mixing is carried out; the preparation method comprises the following steps: sodium alginate solution preparation, shaddock peel nanofiber preparation and composite sodium alginate antibacterial film liquid preparation. The edible composite sodium alginate antibacterial film liquid can be used for fresh keeping of fresh-frozen ducks and has the advantages that moisture permeability of a film can be reduced, so that water loss of the duck meat is controlled and a biological antibacterial agent contained in the edible composite sodium alginate antibacterial film liquid can restrain the growing of putrefying bacteria in the fresh-frozen ducks. Therefore, color, luster and flavor of duck meat can be kept, and the shelf life of the fresh-frozen duct is prolonged.

The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for diagnosing or predicting cancer in a patient. In one embodiment, a method for identifying a patient as having cancer comprises the steps of (a) providing a formalin-fixed, paraffin-embedded or fresh frozen sample of patient tissue; (b) steaming the sample in antigen retrieval buffer; (c) incubating the sample in hydrochloric acid (HCl); (d) incubating the sample with an affinity reagent specific for 5hmC under conditions to form a complex between the affinity reagent and 5-hydroxymethylcytosine (5hmC) present in the sample; (e) detecting the complexes formed between 5hmC and the affinity reagent with secondary detection reagents; (f) quantifying 5hmC levels; and (g) identifying the patient as having cancer if the 5hmC levels in the sample are reduced as compared to a control.

The invention discloses an antifreeze agent for fresh frozen shrimp. The antifreeze agent is characterized in that an aqueous solution system contains the following components according to mass concentration: 5-10% of edible colloid and protein saccharified reactant and 1-5% of erythritol. The antifreeze agent disclosed by the invention has the characteristics of being low in calorie, low in sweetness, free from phosphorus and the like, and can reduce the water losing effect of the fresh frozen shrimp after unfreezing. The antifreeze agent can be used for the long-time frozen storage of the fresh frozen shrimp, and can also be used for other antifreeze agents for freezing denaturation of protein of other aquatic products.

The invention discloses extraction and high-performance liquid detection methods of beta-ecdysone in euphausia superba. Fresh frozen euphausia superba is taken as a material, subjected to unfreezing and tissue cutting treatment, ultrasonic extraction with methanol and degreasing with n-hexane and frozen at subzero 20 DEG C for removal of other impurities, and an extract containing beta-ecdysone isobtained; a high-performance liquid detection method is adopted for detecting the beta-ecdysone in the euphausia superba under the conditions of a UV/Visible detector, a YMC-PACK ODS-A250*4.6 mml.D.Schromatographic column, mobile phase methanol-water, isocratic elution and detection wavelength 248 nm, the peak area is tracked and integrated by Breeze software, and the content of the beta-ecdysone in the euphausia superba is obtained. The operation method is simple and quick and the result is accurate and reliable.

A refrigerator with antistaling-freezing chamber is composed of a main body with freezing chamber, cold storage chamber and their gates, a case body with insulating part to form the case, air suckinginlet and air exhaust outlet, an antistaling-freezing chamber formed by communicating said air exhaust outlet with air sucking inlet of freezing chamber, and a temp regulator for regulating the temp to one of cooling temp levels. Its advantage is controllable food storing temp according to food characters.

The invention relates to the field of fresh-keeping of Chinese medicinal materials, and discloses a fresh frozen liquid, a fresh-keeping method thereof and an application thereof. The fresh frozen liquid comprises water-soluble chitosan, glycerin, trehalose and calcium chloride; the fresh-keeping method comprises the following steps: (1) pre-cooling the fresh frozen liquid under the condition of -1 DEG C to -7 DEG C; (2) placing fresh Cordyceps sinensis in the pre-cooled fresh frozen liquid in step (1) for 5 to 30 minutes; and (3) placing the fresh Cordyceps sinensis after the immersion treatment in step (2) at -1 DEG C to -7 DEG C under refrigeration. The fresh Cordyceps sinensis is treated with the fresh frozen liquid is fresher than that of a direct refrigerator frozen-storage method, and the preservation time is extended by at least 14 days.

The invention provides a making method of dried chicken feet. The making method comprises steps as follows: fresh-frozen or frozen chicken feet are taken as raw materials, the unfrozen chicken feet are soaked with an aqueous solution of a chicken foot peeling agent and then are fished out, after claws and bones are removed manually, rice vinegar and a lemon liquid are added, and the chicken feet are rolled and rubbed at a low speed and are soaked in an environment at 0-4 DEG C; after rolling, rubbing and soaking, the chicken feet are wrapped with crisp powder, are dried with a high-low-temperature air frying technology and are bagged, packaged and put in storage through matching with self-made powder spraying bags and are stored at the normal temperature. The chicken foot peeling agent is independently developed, and after the chicken feet are soaked with the chicken foot peeling agent, the bones are removed more easily and thoroughly; with the adoption of the efficient and standard manual claw and bone removing method, the problem about bone removal of the chicken feet is solved, and the bone removing efficiency is improved; by means of the chicken foot fishiness removing method with the rice vinegar and the lemon liquid, fishiness, odor and stink of the chicken feet can be removed effectively; a product obtained after fishiness removal not only is free of fishiness and other odor, but also has slight lemon fragrance.

Compositions/methods for employing fresh-frozen or FFPE colon cancer tissue in left side colon cancer (LCC) and right-side colon cancer (RCC) disease patients for risk of relapse assessment/stratification is provided (3 strata and a 4 strata methodology). An RCC gene panel of 4 genes (FAM69A, CDX2, FAM84A, ITGA3), and 9 genes (FAM69A, CDX2, ITGA3, FAM84A, ITPRIP, RAB3B, SMAD3, PCSK5, MMP28), is provided. An LCC gene panel of 4 genes (NOX4, WNT5A, MMP3, IBSP), and a 9 genes (MMP3, WINT5A, NOX4, IBSP, SLC16A6, CYPIBI, TFAP2C, MATN3, ANKRD6), is provided. A microchip-based clinical tool, and a kit including a microchip, is presented. The invention also describes a computer-implemented method for assessing relative risk of relapse in LCC and/or RCC disease. An individual patient scoring method that presents a continuous stratification score useful in the post-surgical colon cancer management of LCC and/or RCC patient is also presented.

A wild edible bolete processing method is characterized by comprising the steps of carrying out low temperature processing on fresh-frozen white boletus, red boletus and boletus aereus for 4-5h at -30 DEG C to -35 DEG C; then slightly unfreezing quick-frozen bolete for 3-4h at -20 DEG C to -25 DEG C; frying the unfrozen bolete in a high-pressure fryer at 90 DEG C-110 DEG C, wherein the pressure inside the high-pressure fryer is 70KPa-80KPa, pure-natural colza oil is adopted as frying oil, and the oil frying time is 9-11min; fishing up the fried bolete, and then packaging the bolete. According to the wild edible bolete processing method, the bolete does not have toxic and side effects after being processed, the potential safety hazard does not exist, and the instantly edible wild bolete can be processed by adopting a common cooking method.

The invention discloses a salt-baked chicken neck and a preparation method thereof. The preparation method comprises the following technical steps: firstly, receiving raw materials: selecting fresh frozen skinless chicken necks which are from a disease-free district and meet the requirements, and strictly controlling bacteria and pesticide residues; secondly, thawing raw material meat: putting the raw material chicken necks into a thawing room for thawing, and controlling the temperature of the thawed raw material chicken necks at 0DEG C to 5DEG C; thirdly, tumbling ingredients: performing ingredient tumbling on the thawed raw materials in time, wherein the ingredients are salt-baked marinade, baking soda, composite phosphate, ethyl maltol, soybean oil and capsanthin; controlling the temperature of the raw materials at 0DEG C to 8DEG C during tumbling and tumbling for 25 minutes. The salt-baked chicken neck disclosed by the invention is mainly prepared by pickling frozen chicken necks by using the ingredients, and is deeply favored by people; the appetite also can be increased; the salt-baked chicken neck has the advantages of unique taste, good mouthfeel, high nutritive value, easiness in preservation and convenience in eating.

The invention discloses primers, a Taqman probe and a kit for detection of STMN1 mRNA expression quantity, wherein the primers have the nucleotide sequences shown in SEQ ID No:1-2, and the Taqman probe has the nucleotide sequence shown in SEQ ID NO:3; a 5' end of the probe is labeled with a fluorescent reporter group, and a 3' end of the probe is labeled with a quencher. The primers and the probe have high sensitivity and good specificity; the kit composed of the primers and the probe is easy and safe to operate, has the whole detection process requiring only 80 min, saves the time, has relatively high sensitivity and specificity on all of fresh frozen tissues, paraffin-embedded tissues, blood and other samples, and has wide application range; relative quantification adopts a double-standard-curve method, an error is relatively small, and a result is more accurate.

The invention discloses a method used for detecting monoclonal antibody drug tissue cross-reactivity with fresh frozen tissues. The method comprises following steps: 1) fresh frozen tissues are collected; 2) fresh frozen tissue samples are prepared; 3) the fresh frozen tissue samples are made into fresh frozen tissue slices; and 4) the fresh frozen tissue slices are used for detecting monoclonal antibody drug tissue cross-reactivity. The method is capable of improving frozen tissue slice-making technology and slice-making quality, reducing difficulty of immune response evaluation, is convenient for operation, and is high in application value.

Methods for tissue microdissection- or dissociation-free means of collecting cell-specific biomolecules from complex heterogeneous tissue sources including native tissues, fresh frozen, fixed, and archived specimens. The tissue section having a plurality of beads with immobilized capture probes on their surface evenly distributed over all cell types of the tissue is arranged. Alternatively the tissue section being coated with a thin layer of resin containing incorporated or covalently bound immobilizing capture probes is arranged. The beads or resin are incubated directly on the tissue section permitting capturing of the biomolecules by the capture probes. After incubation, the tissue section is visualized and the cells or area of interest are identified. Next, the beads or resin samples, located in these cells or areas of interest, are collected and transferred to a sample tube where biomolecules are separated from the beads or resin using standard methods and are then used in downstream applications.