Prepn. of blood coagulation factor IX compound

A blood coagulation factor and complex technology, which is applied in the field of preparation of blood coagulation factor IX complex, can solve the problems of poor mechanical strength, low recovery rate, and difficult to stabilize quality, and achieve the effects of reducing risk, improving recovery rate, and simple operation

Inactive Publication Date: 2002-02-20
四川高维系统工程技术有限公司 +2
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AI Technical Summary

Problems solved by technology

[0004] It is found in practice that the DEAE-sephadex A-50 resin adopted or recommended in many of the above-mentioned preparation methods has poor mechanical strength, short service life, difficulty in separating the glue, low recovery rate, and difficult production operations, making the finished product The cost is high and the quality is difficult to stabilize

Method used

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  • Prepn. of blood coagulation factor IX compound
  • Prepn. of blood coagulation factor IX compound
  • Prepn. of blood coagulation factor IX compound

Examples

Experimental program
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Embodiment Construction

[0017] What example 1~example 10 adopts is common column chromatography mode, and its operation method is: in the DEAE-sepharose Fast Flow gel chromatography column that swells and packs the column in advance, use 4-5 times of glue-like volume Corresponding phosphate buffer or citrate buffer, equilibrate the chromatographic exchange column in a conventional manner. Then, the fresh frozen human plasma was thawed at 37°C in a rapid way so that the fibrinogen would not be precipitated and adjusted to pH 7.0 with the same system buffer solution, injected into the chromatography exchange column and kept standing in the corresponding conditions listed in each case. time to complete the adsorption exchange process. The column was then washed with an appropriate buffer of the same system at a flow rate of 2.5 ml / min. After the washing peak is over, use the buffer solution of the same system adjusted to the required ionic strength with sodium chloride to elute the chromatographic exch...

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Abstract

The present invention relates to the preparation of plasma thromboplastin antecedent IX complex phosphate buffer solution or citrate buffer solution with 4-7 milli mole concertration and pH 5.5-6.5 is used to balance the gel used for adsorption, with fresh frozen human plasmia being added. DEAE-sepharose Fast Flow is used to proceed ion exchange adsorption separation, then same buffer solution with pH 5.5-7.0 is used to wash adsorption gel. further same buffer solution with pH 6-8 is used to elute adsorption gel, collect plasma thromboplastin antecedent IX complex containing factor II, VII, IX and X.

Description

technical field [0001] The present invention relates to an improved method for preparing blood coagulation factor IX complex, specifically ionizing fresh frozen plasma with DEAE-sepharose Fast Flow (diethylamine ethyl-agarose fast glue) gel The method of exchange adsorption separation. Background technique [0002] For hemophilia B and abnormal coagulation function caused by liver disease, high-purity human blood coagulation factor IX is mainly used at present, or coagulation factor IX complex mainly containing coagulation factors II, VII, IX, and X dependent on vitamin K (ie "prothrombin complex") plasma protein products. Since the latter product contains a variety of coagulation factors, it can also be used for the treatment of bleeding caused by low levels of factors II, VII, IX, and X due to vitamin K deficiency, and has great application value. [0003] Whether it is the preparation of a single blood coagulation factor IX or the method of preparing a prothrombin compl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/14A61K38/43A61P7/04C07K1/14C07K1/16C07K14/745
Inventor 余蓉杨继虞李晓红
Owner 四川高维系统工程技术有限公司
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