Manufacturing method of gradient tissue engineering scaffold

Abstract

The invention provides a manufacturing method of a gradient tissue engineering scaffold. The manufacturing method comprises the following steps: printing a biological high molecular material and hydrogel into three types of grids by utilizing a low-temperature deposition 3D (Three Dimensional) printer, so as to obtain a hierarchical structure which comprises a tangential grid corresponding to superficial-layer tangential fibers, a uniform grid corresponding to middle-layer transition fibers and a normal grid corresponding to bottom-layer normal fibers; forming a grid bracket, wherein the grid bracket has the mechanical strength characteristics that a compression resisting force is gradually increased from shallow to deep and an anti-shearing force is gradually reduced from shallow to deep; after printing, carrying out freeze drying on the grid bracket for a first time; adding a dECM (decellularized Extracellular Matrix) solution into pores of the formed grid bracket; carrying out freeze drying for a second time and removing a solvent component in the dECM solution, so as to obtain the composite gradient tissue engineering scaffold which is close to a normal cartilage directional micro-tissue. According to the manufacturing method, one-step molding of the gradient composite scaffold can be realized and a bionic effect is remarkably optimized; quantitative mechanical strength optimization can be carried out very well.

Description

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AI Technical Summary

Benefits of technology

This patented describes an improved way to make biological materials that have specific properties such as high tensile stress resistance or porosity. These modifications help improve their ability to repair damaged areas on human body's own cells called chondrocytes. They use special techniques like three dimensional printing (3DP) and directed ice crystal growth to create these structures quickly without causing damage during production. By combining advanced methods together, they allow scientists to optimize them over time while still maintaining good quality material.

Problems solved by technology

This patented technical problem addressed by this patent relates to improving the mechanical property required for implant devices made from synthetic polymers like poly(propylene carbonate)-based plurality of different types of molecules called chitosan, collagen, elastin, protcolysaccharide, etc., while maintaining good biocompatibility and compatibility between these components.

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