10results about How to "Suitable for clinical application" patented technology

The invention discloses a bone cement composite material with collapse resistance and a preparation method thereof, and belongs to the technical field of biomedical materials. The composite material comprises a solid phase part and a liquid phase part, the liquid phase part and the solid phase part are mixed according to the liquid-solid ratio of 0.35 mL / g to 0.6 mL / g; the solid phase part is composed of calcium sulfate powder and calcium phosphate powder, and the mass ratio of the calcium sulfate powder to the calcium phosphate powder is 85: 15; the liquid phase part is selected from carboxymethyl chitosan or a combination of carboxymethyl chitosan and sodium alginate. The bone cement composite material prepared by the preparation method disclosed by the invention has good biocompatibility; due to a gel network formed by carboxymethyl chitosan and sodium alginate, the compressive strength of the bone cement is remarkably improved, and the collapse resistance and injectable performance are excellent; meanwhile, the bone cement composite material promotes the attachment and proliferation of osteoblasts, and can induce osteogenic differentiation in vitro.

This invention belongs to the field of biomedicine and regenerative medicine technology. It discloses a chimeric polypeptide, related biomaterials, and their application in inducing cardiomyocytes. The chimeric polypeptide is obtained through family homologous domain substitution and is selected from the following groups: (1) a MEF2 chimeric polypeptide with MEF2C protein as the backbone, wherein the N-terminal and C-terminal transcriptional activation domains are replaced by homologous regions of MEF2B and MEF2D, respectively; (2) a GATA chimeric polypeptide with GATA4 protein as the backbone, wherein the N-terminal transcriptional activation domain is replaced by homologous regions of GATA1 and GATA6. This invention also provides the nucleic acid encoding the polypeptide, the construct, and a method for efficiently inducing fibroblast reprogramming into cardiomyocytes under in vitro conditions. When the chimeric polypeptide of this invention is used alone or in combination, the induced cells exhibit calcium ion oscillations earlier and have higher maturity.

The application provides a kind of mitochondria-targeted palladium-doped finger citron carbon dot nanomaterial and a preparation method thereof.The preparation method uses natural plant finger citron as raw material, through hydrothermal reaction, cooling, centrifugation, dialysis, freeze-drying and other steps, extracts natural carbon dots from finger citron, and then dopes palladium and mitochondria-targeted peptide, finally obtains mitochondria-targeted palladium-doped finger citron carbon dot nanomaterial (PDs@SS31).The mitochondria-targeted palladium-doped finger citron carbon dot nanomaterial prepared by the application can form a multifunctional synergistic treatment system in combination with a near-infrared light irradiation device.The system has high-efficiency ROS scavenging, precise mitochondria targeting, near-infrared response and immune regulation functions, has excellent biocompatibility, can effectively activate the mitochondrial autophagy pathway, and can significantly improve the treatment effect of acute lung injury by specifically reprogramming the oxidation-reduction homeostasis in the lung microenvironment.

The application relates to a hydrogel for regulating a cGAS-STING path by mitochondria autophagy and a preparation method and application thereof, the preparation method comprising the following steps: preparation of spirulina loaded with deferoxamine and preparation of the hydrogel; the drug-loaded hydrogel system of the application has the functions of wet tissue adhesion and multi-dimensional pathological microenvironment regulation, fully utilizes the effects of the components, realizes regulation of cell fate from a molecular level, induces macrophages to polarize into M2 type, targets inhibition of a cGAS-STING path and ferroptosis, and remolds an immune microenvironment of a wound surface, thereby providing a new strategy and idea for repair of a burn infection wound surface.

The invention is applicable to the field of genetic engineering, and provides a mycobacterium tuberculosis 32C-PPE18-LS protein nanoparticle, a preparation method and application thereof, the mycobacterium tuberculosis 32C-PPE18-LS protein nanoparticle is formed by sequence fusion of a C terminal of a mycobacterium tuberculosis MTB32A protein, a PPE18 protein and an LS protein, and the amino acid sequence of the mycobacterium tuberculosis 32C-PPE18-LS protein nanoparticle is shown in a sequence table SEQ ID NO: 2. According to the mycobacterium tuberculosis 32C-PPE18-LS protein nanoparticles provided by the invention, multi-fragment immunogenic protein fusion is adopted, a synergistic immune effect can be induced, and the immunostimulation ability of an antigen is remarkably enhanced. Moreover, the 32C-PPE18-LS protein nanoparticles are non-toxic and free of pathogenicity, so that the vaccine can be ensured to have good biological safety, and the 32C-PPE18-LS protein nanoparticles are suitable for clinical application.

The invention is applicable to the field of gene engineering, and provides a mycobacterium tuberculosis RpfB-LS protein nanoparticle, a preparation method and application thereof, the mycobacterium tuberculosis RpfB-LS protein nanoparticle is formed by sequence fusion of mycobacterium tuberculosis RpfB protein and LS protein, and the amino acid sequence of the mycobacterium tuberculosis RpfB-LS protein nanoparticle is shown in a sequence table SEQ ID NO: 2. The RpfB-LS protein nanoparticles provided by the invention are non-toxic and free of pathogenicity, can ensure that vaccines have good biological safety, and are suitable for clinical application. Animal experiments show that after a mouse is immunized by the RpfB-LS protein nanoparticle, strong humoral immunity (antibody generation) and cellular immunity (T cell response) can be stimulated at the same time, and it is proved that the recombinant RpfB-LS protein nanoparticle vaccine can effectively induce specific immune protection.

This invention discloses a kit for detecting HPV integration sites, belonging to the field of genetic engineering. Using this invention, targeted sequencing of partially unknown sequences can be performed at very low sequencing throughput (approximately 400K-600K) to obtain sufficient data (approximately 50X coverage depth) to yield reliable results. It can detect HPV integration in samples including, but not limited to, cervical cancer samples. Another objective of this invention is to verify the integration status of primer-corresponding regions using methods such as hybridization, quantitative fluorescence sequencing, or first-generation sequencing, employing single or partial primers provided by this invention. The presence of HPV integration strongly suggests that the patient's condition is CIN and cervical cancer. This invention provides more data support for the clinical research and diagnosis of cervical cancer from the perspective of HPV integration.

The present application provides an ultrasonic color imaging method, system, apparatus, computing device and computer-readable medium. The method includes: receiving multiple ultrasonic receiving signals provided by an ultrasonic transducer, wherein the center frequencies of the multiple ultrasonic receiving signals are different from each other; dividing the multiple ultrasonic receiving signals into a preset number of groups, wherein the preset number is equal to the number of channels of a preset color mode; and using each group of ultrasonic receiving signals as a component of one of the channels of the preset color mode and generating a color image according to the preset color mode. The method generates a color image by using multiple ultrasonic receiving signals with different center frequencies as channel components of the color mode, which can make full use of the color perceptible space of the human eye to more completely and accurately reflect the information of the target object, and the required calculation amount is low, the hardware performance requirements are low, and it is more suitable for clinical applications.

The present invention belongs to the field of medical detection, and discloses a primer set, a kit and a system for rapid diagnosis of PWS / AS. The present invention uses MS-PCR in combination with NGS sequencing technology to detect the methylation level of the SNRPN gene in the 15q11.2-q13 region of chromosome 15. The detection method can be used for rapid molecular diagnosis of Prader-Willi syndrome (PWS) and Angelman syndrome (AS) epigenetic inheritance. The method is easy to operate, has low detection cost, good detection result accuracy, high detection sensitivity and specificity, and high detection throughput, which is suitable for clinical promotion and application. The detection results of this method can accurately distinguish the abnormal methylation level of the chromosome region 15q11.2-q13, can be used for rapid auxiliary diagnosis of PWS syndrome and AS syndrome, and is suitable for clinical rapid diagnosis applications.

The invention relates to the technical field of biomedical materials, in particular to a biomass hydrogel dressing based on broccoli stem pectin and a preparation method and application thereof.The preparation method comprises the following steps that polyvinyl alcohol is dissolved in deionized water and stirred to be uniform, and a polyvinyl alcohol solution is obtained; and adding the broccoli stem pectin, adjusting the pH value to 2.0-3.0, stirring at 35-60 DEG C for 0.1-2 hours until the broccoli stem pectin is completely dissolved, pouring the obtained mixed solution into a mold, freezing, unfreezing, repeating for 2-5 times, packaging the obtained hydrogel in a dialysis bag, and dialyzing in neutral water for 3-6 times until the pH value is 7.0 to obtain the hydrogel dressing. According to the broccoli stem pectin-based biomass hydrogel dressing as well as the preparation method and the application thereof, the broccoli stem pectin is dissolved in the polyvinyl alcohol solution to be repeatedly frozen and thawed to prepare the hydrogel dressing, and the hydrogel dressing has excellent biocompatibility, mechanical property and biological activity and a good tissue repair function.