20results about How to "Good detection repeatability" patented technology

This invention relates to the fields of environmental monitoring and biosafety technology, and in particular to a viral aerosol sampling patch for face masks, comprising: a capture layer for intercepting viral aerosol particles in the air; a culture layer disposed inside the capture layer, the culture layer comprising a culture unit supporting the virus to maintain activity and replicate in the presence of a host, a host bacterium sensitive to the virus, and a visual indicator indicating viral replication or host response; and a base layer disposed inside the culture layer; the sampling patch is fixed to the face mask through the base layer, and viral aerosol collection is achieved without external power through the capture layer, and countable detection results are generated after sampling through the culture layer; utilizing the airflow and humidity conditions naturally formed during wearing, effective interception, activity preservation, and visual detection of viral aerosols are achieved without complex equipment and additional operating steps, and the operation is simple and repeatable.

The application provides a method for detecting biochemical oxygen demand in sewage, and belongs to the field of oxygen demand detection. The method comprises the following steps: forming a fluorine-containing organosilicon nano coating on the electrode surface of a dissolved oxygen sensor of a full-automatic BOD5 analyzer to obtain a fluorine-silicon modified dissolved oxygen sensor; pretreating the sewage to be detected in an adsorption column composed of modified activated carbon and porous silica gel to obtain a pretreated water sample; fixing bacillus subtilis on a porous carrier and then putting the carrier into the pretreated water sample to obtain an inoculated water sample; measuring the initial dissolved oxygen of the inoculated water sample and the end-point dissolved oxygen after 5-day culture; and obtaining the five-day biochemical oxygen demand in the sewage according to the initial dissolved oxygen and the end-point dissolved oxygen. Through the four-level collaborative system of "sensor anti-interference optimization, sample purification and enrichment, microbial reaction standardization and accurate signal acquisition", the application systematically eliminates the error sources in the traditional BOD5 detection, and realizes the accurate measurement of the organic matter pollution level in complex sewage.

The present application relates to the technical field of microfluidic detection, and discloses a microfluidic detection system and a liquid detection method, wherein the microfluidic detection system comprises a paper-based microfluidic chip with a detection cell (21) and a regulation unit, the regulation unit is configured to regulate at least one of the environmental temperature, air flow speed, humidity and vacuum degree of the area where the detection cell is located, so as to regulate the limited evaporation speed of the liquid in the detection cell through the vent hole. According to the migration mechanism of colorimetric on the paper chip, the evaporation speed of different positions of the detection cell is regulated by covering the non-water-permeable and air-permeable part with a vent hole on the detection cell, and at the same time, the evaporation speed of the liquid in the detection cell through the vent hole is regulated by using the regulation unit, so as to effectively improve the efficiency of the colorimetric migrating and enriching with the supplement of moisture to the position with faster evaporation speed in the evaporation process, improve the detection sensitivity and reduce the detection limit.

This invention belongs to the field of chemical detection technology, specifically relating to a method for detecting diethylstilbestrol (DES) based on a photothermal probe and lateral chromatography test strip. The method comprises a photothermal material coupled with a DES monoclonal antibody. The detection zone of the lateral chromatography test strip is coated with a PBS solution containing DES antigen, while the control zone is coated with a PBS solution containing goat anti-mouse secondary antibody. This invention combines the photothermal properties of the photothermal material with lateral chromatography test strip technology to achieve sensitive quantitative analysis of DES. In this invention, an 808nm laser is used to irradiate the control and detection zones of the lateral chromatography test strip, and a thermal imager is used to record the temperature changes in each zone. A standard curve is established using the temperature difference and DES concentration to achieve quantitative analysis of DES. This invention can be used for rapid, accurate, intuitive, highly repeatable, and highly stable quantitative detection of samples containing DES.

This invention relates to the field of mortar testing, specifically to a mortar consistency testing device for environmentally friendly mortar. It includes a lead screw slide with its output end fixed to a worktable. A cover is located beside the worktable, and a cone-shaped device coaxially and detachably holds the mortar. A vibration mechanism is located at the lower end of the cover. An infrared sensor is fixedly connected to the lead screw slide near the cover, and the infrared sensor is electrically connected to a controller. An electromagnet is fixedly connected to the lead screw slide below the sensor. A support is fixedly connected to the lead screw slide below the electromagnet, and an electric actuator is fixedly connected to the upper end of the support. A retaining ring is fixedly connected to the end of the support away from the lead screw slide, and a guide rod is coaxially connected to the retaining ring. A permanent magnet is fixedly connected to the upper end of the guide rod near the lead screw slide, and a cone-shaped device coaxially with the cone-shaped device is fixed to the lower end of the guide rod. During testing, the cone is filled with mortar and fixed coaxially with the cover cylinder. The vibration mechanism disperses the mortar. The screw slide and electric actuator adjust the position of the cone, and the electromagnet attracts the guide rod. After the power is cut off, the cone falls, the infrared sensor detects the displacement and transmits it to the controller to calculate the consistency, thus achieving accurate testing.

The invention belongs to the technical field of sensor array detection, and provides a sensor array device based on stepping scanning and a detection method, and the method comprises the steps: collecting signals through the comparison of a test group and a control group, and precisely judging whether aliasing occurs or not through the analysis of spectrum peak value and time domain performance; further defining a risk super-harmonic frequency band through multi-gradient driving fundamental frequency test, and verifying and positioning a super-harmonic order and an interference source in combination with dynamic fine tuning; and finally, eliminating aliasing by adopting a driving parameter fine tuning or reverse digital compensation mode according to working condition limitation, and extracting a real detection signal. The device adaptive to the method adopts a modular design, and all modules cooperate and are efficient, so that the device can be stably adaptive to a high-speed stepping working condition; according to the invention, accurate determination, positioning and elimination of aliasing are realized, the detection accuracy and stability are improved, different process requirements are adapted, and the detection error is reduced.

The invention provides a railway four-power digital engineering spatial topology geometry detection method and system, and the method comprises the steps: obtaining railway four-power digital engineering multi-source spatial data and a model, carrying out the graph-model separation and preprocessing, and unifying the data to a same coordinate system; constructing a three-dimensional model comprising a line, a structure and four-electric equipment on a BIM or GIS platform; establishing connectivity and inclusion topological relations among components including points, lines, surfaces, bodies, equipment, cables, bridges and cabinets in the model, and extracting related geometric features; on the basis of the topological relation and the geometrical characteristics, collision, gap and distance detection is automatically executed according to specifications, detection results of problem components and parameters are generated, and highlighted display and report output are carried out in a three-dimensional visual interface; the corresponding system is composed of a data acquisition and preprocessing module, a three-dimensional model construction module, a spatial topological relation establishment module, a spatial geometric information detection module and a result display module. The automation and precision of spatial geometric detection of railway four-electric digital engineering can be greatly improved.

This invention discloses a detection kit technology, specifically a detection kit and method for novoarginine, comprising a universal sample processing solution, a novoarginine-specific probe, fluorescently labeled streptavidin, washing solution, chromogenic substrate solution, stop solution, novoarginine standard, and a reaction microplate. The universal sample processing solution is composed of acetonitrile, potassium dihydrogen phosphate buffer, and disodium ethylenediaminetetraacetate in a volume ratio of 3:6:1, with the potassium dihydrogen phosphate buffer concentration being 0.1 mol / L and the disodium ethylenediaminetetraacetate concentration being 0.05 mol / L. The novoarginine-specific probe is a biotin-labeled novoarginine monoclonal antibody at a concentration of 1 mg / mL. The surface of the reaction microplate is coated with novoarginine hapten. This invention, by developing a universal sample processing solution adaptable to different matrix samples, eliminates the need for customized pretreatment procedures, significantly improving the versatility of the kit and solving the cumbersome problem of requiring separate processing for different samples.

This invention relates to the field of light source technology, and discloses an optical inspection system integrating multi-angle prism coupling and beam splitting fusion. The system includes: a housing with an installation space and a detection space within it, and a detection port communicating with the detection space on the housing; a camera fixedly connected to the installation space; a semi-transparent, semi-reflective lens disposed below the detection space; a point light source assembly disposed within the detection space, below the semi-transparent, semi-reflective lens; and a first prism and a second prism disposed above the semi-transparent, semi-reflective lens, which are tilted at 45° to the upper side of the detection space in a horizontal projection. A detection method is also provided. This invention achieves multi-field synchronous imaging of the bottom and two sides of a workpiece using a single camera and a single exposure. The three optical paths are independent and do not interfere with each other after precise optical path calibration.

This utility model relates to the field of mechanical manufacturing technology and provides an automatic inspection mechanism for sleeves. The sleeve has a conical hole. The automatic inspection mechanism includes: an operating table on which a feeding unit, a conveying unit, a first inspection unit, a second inspection unit, a third inspection unit, and a sorting unit are arranged. The feeding unit is used to convey the sleeve to be inspected to a preset position. The conveying unit is used to transport the sleeve. The first and second inspection units are used to inspect the outer diameter and the conical hole of the sleeve, respectively. The third inspection unit is used to perform visual inspection on the sleeve. The sorting unit is used to classify and collect the sleeves that have completed the inspection. In the second inspection unit, the coordinated cooperation between the inspection rod and the pressure block not only realizes the high-precision insertion measurement of the conical hole, but also ensures the positioning stability during the inspection process through the clamping force applied by the pressure block, which significantly improves the inspection accuracy and repeatability.

This invention discloses a waterproof testing device and method based on the processing of waterproof membranes for high-speed railway tunnels, belonging to the field of waterproof testing technology. The waterproof testing device based on the processing of waterproof membranes for high-speed railway tunnels includes: a testing machine; a variable frequency speed-regulating pump fixedly connected to one side of the testing machine; one end of the variable frequency speed-regulating pump connected to a liquid delivery channel; a diaphragm accumulator connected to one side of the liquid delivery channel; and a multi-state holding device for fixing the waterproof membrane and pressing it onto a testing platform. The multi-state holding device moves in the sequence of a fixed position, a puncture position, and a dynamic and static water pressure testing position. This waterproof testing device and method based on the processing of waterproof membranes for high-speed railway tunnels, through a flat-push switching between fixed position clamping, puncture position testing, and dynamic and static water pressure testing, eliminates the need for repeated disassembly and assembly of the waterproof membrane. Only the threaded rod needs to be turned to achieve synchronous downward movement and secondary clamping of the upper and lower clamps, shortening the testing cycle and improving data accuracy.

The invention belongs to the technical field of immunofluorescence detection, and particularly discloses an ultra-sensitive immunofluorescence detection method. Aiming at the problems of low sensitivity, poor repeatability and high cost of the existing method, the method comprises the following steps: capturing a target by adopting coated antibody-labeled magnetic microspheres, sequentially reacting with a biotin-labeled detection antibody and streptavidin-labeled horse radish peroxidase, and carrying out signal amplification dyeing by catalyzing casein fluorescent dye activation through peroxidase. And finally, percolating by using a microporous filter membrane or enriching by using a micro-fluidic chip, and detecting by using a fluorescence reading instrument or a microscope. According to the method, through multi-stage signal amplification and enrichment concentration, ultra-high-sensitivity detection below the picogram level is realized; the magnetic microspheres are used as reaction carriers, rapid separation, purification and washing are realized through magnetic field control, and the repeatability is remarkably improved; meanwhile, the antibody dosage is greatly reduced, the equipment requirement is lowered, and high sensitivity, high repeatability and economical efficiency are achieved.

This invention provides a Skin-Resolved Diffusion (SERS) Detection System for Deep-Resolved Drug Diffusion, comprising a SERS-active microneedle array and a confocal Raman microscope. The SERS-active microneedle array is used for in-situ drug detection at multiple depths in the skin. It consists of polymer microneedles of varying lengths, with noble metal core-shell nanostars uniformly immobilized on their surfaces. Each noble metal core-shell nanostar comprises a noble metal nanostar core modified with an internal standard reporter molecule and an outer noble metal shell. The confocal Raman microscope records the SERS signal generated after the microneedles penetrate the skin. A quantitative model is established by comparing the peak ratio of the target drug characteristic peak to the internal standard characteristic peak in the SERS signal to determine the drug concentration at different depths. This invention enables quantitative analysis of the diffusion behavior of topical drugs at different skin types and depths.

The invention provides a creatine kinase isoenzyme freeze-dried product as well as a preparation method and application thereof. The creatine kinase isoenzyme freeze-dried product is prepared from the following raw materials: a creatine kinase isoenzyme antigen, polygonatum polysaccharide and a chelating agent; the chelating agent comprises any one of citric acid, iminodiacetic acid and 1-hydroxyethylidene-1, 1-diphosphonic acid. The components such as the polygonatum polysaccharide and the chelating agent are adopted, so that double protection of oxidation resistance and dimer stability is realized, and the creatine kinase isoenzyme is good in activity after freeze-drying, stable in long-term storage and good in detection accuracy and repeatability.

The invention discloses a traditional Chinese medicinal material pesticide residue detection device, and relates to the technical field of traditional Chinese medicinal material residue detection, the traditional Chinese medicinal material pesticide residue detection device comprises an outer shell and an annular operation table mounted in the outer shell, and a conical grinding bin, a reagent storage tank and a spectrum detection instrument are sequentially arranged right above the annular operation table in the clockwise direction; three assembling grooves are formed in the annular operation table, a sample test tube is sleeved with each assembling groove, a gear assembly is installed at the bottom of the annular operation table, and the annular operation table is driven by the gear assembly to rotate intermittently; the three sample test tubes are sequentially switched to the positions under the conical grinding bin, the reagent storage tank and the spectrum detection instrument, continuous operation of traditional Chinese medicinal material residual liquid sampling, reaction reagent filling and spectrum detection is sequentially completed, multiple samples with gradually-increased concentrations are obtained through grinding pressure gradient regulation and control, the reagent filling amount is adjusted, and the detection accuracy is improved. The detection accuracy is comprehensively improved along with dynamic matching of sample concentration.

The invention discloses a rabbit-derived single B cell antibody of porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein, a fusion protein, a detection kit and a detection method. The invention provides two rabbit-derived single B cell antibodies T-11 and T-12, wherein amino acid sequences of variable regions of a heavy chain and a light chain of the rabbit-derived single B cell antibodies T-11 and T-12 are respectively shown as SEQ ID No.1-4; also provided are PRRSV-1 type and type 2 nucleocapsid protein fusion proteins BiN-2 (SEQ ID No.5) and antibody compositions comprising T-11 and T-12. Based on the antibody and the fusion protein, a competitive ELISA detection method and a kit are established, T-11 is used for capturing BiN-2 to construct an indirect coating antigen, biotin-labeled T-12 is used as a competitive antibody, and a judgment result is detected through an enzymatic reaction and a light absorption value. The method can be used for simultaneously detecting the PRRSV-1 type and the PRRSV-2 type antibodies, the sensitivity and the specificity both reach 97% or above, the method has no cross reaction with other porcine virus antibodies, the operation is simple and convenient, the stability is good, and a reliable tool is provided for accurate prevention and control of PRRS and epidemiological monitoring.

The invention discloses a high-temperature tempered glass ink expansion coefficient detection method and a sample preparation mold. The sample preparation mold comprises a pressing rod, a mold inner core, a mold sleeve and a movable base. The pressing rod is inserted into the mold inner core and is movably connected with the mold inner core; the mold sleeve wraps the outer surface of the mold inner core and is movably connected with the mold inner core; the movable base is positioned at the bottom of the mold inner core and the mold sleeve in the vertical direction and is movably connected with the mold inner core and the mold sleeve. The error of the test result after sample preparation does not exceed + / -0.2, the accuracy is high, the expansion coefficient detection repeatability is good, and the problem that the ink expansion coefficient cannot be tested or is not accurately tested in the industry is solved.

This invention discloses a detection device for oxygen sensor chip testing, relating to the technical field of oxygen sensor testing equipment. It includes a testing box mounted on a testing platform. The side wall of the testing box has several mounting holes for plug insertion into the oxygen sensor chips. An adjustment pipe connecting to a factory mixed gas pipeline is located on the side of the testing box, and an oxygen pipeline connecting to a factory oxygen supply pipeline is also located on the side of the testing box. Inside the testing box, partitions are slidably arranged along the height direction of the testing box. This detection device for oxygen sensor chip testing, through the use of partitions, flaps, and telescopic components, flexibly achieves zoned isolation control of the testing box, while ensuring full contact between the test gas and the plug. This facilitates rapid and uniform coverage of the sensitive areas of each oxygen sensor chip by the test gas, shortening the response time and improving the consistency and reliability when multiple chips are tested simultaneously.

The application discloses a kind of titanium alloy hydrogen content detection's anti-hydrogen absorption sample preparation and detection method, belong to metallurgical material detection technical field.The application includes: titanium alloy sample is processed into block, after ultrasonic cleaning in acetone is dried in humidity ≤24% environment, and in 4 hours detection or vacuum sealing preservation;Select tin sheet as dissolvent, tin sheet and sample mass ratio is 1:2;Graphite crucible is carried out empty burning pretreatment;Inert gas melting thermal conductivity method detection parameter is set as analysis power 2.7KW, degassing power 4.5KW, analysis time 120 seconds, cooling water temperature ≤28 ℃;Tin sheet is placed in the bottom of crucible, after adding sample, detection is carried out.The application is through anti-hydrogen absorption combined process, tin sheet high-efficiency fluxing and crucible empty burning pretreatment, effectively suppresses titanium alloy hydrogen absorption, realizes sample complete melting, eliminates crucible residual interference, the relative standard deviation of detection result is ≤3%, significantly improves detection accuracy and repeatability, applicable to the precision quality control of hydrogen content in titanium alloy and special steel.

This utility model relates to the technical field of electrical testing equipment, and provides an electrical testing device including a worktable with a support. At least one electrical testing station is mounted on the worktable, and each electrical testing station includes a first linear actuator. At least one detection terminal is provided at the power output end of the first linear actuator. The support is positioned above the first linear actuator and has a designated space for placing the workpiece to be tested. In this utility model, by setting up a support and electrical testing station on the worktable, and using a first linear actuator to automatically raise the detection terminal to contact the workpiece, the electrical testing process is automated, replacing the traditional manual testing method and significantly improving testing efficiency and stability. This structure is rationally designed and reliably operates, not only reducing manual labor intensity but also improving testing accuracy and repeatability.