190results about How to "Good detection repeatability" patented technology

The invention discloses a screw rotor type surface profile error measurement instrument. The screw rotor type surface profile error measurement instrument is characterized in that: a measured rotor is supported between a headstock center and a tailstock center, and the measured screw rotor is driven by rotary working platforms to rotate; and a movable working platform is arranged, a measurement unit is arranged on the movable working platform, a long grating sensor for detecting the position of the movable working platform is arranged in the measurement unit, and a laser displacement sensor is fixedly arranged on an electric rotary disk. According to the screw rotor type surface profile error measurement instrument disclosed by the invention, the original measurement data on the screw rotor type surface feature is acquired under the motion coordination of two rotary working platforms and a one-dimensional translation platform by using the laser displacement sensor, and the corresponding data treatment and coordinate conversion are performed, so that the screw rotor type surface profile error data can be obtained and the error assessment result is given; and the screw rotor type surface profile error measurement instrument is simple in structure and good in processing and assembling performance, and is capable of realizing the surface profile error measurement on various screw surfaces and compatibly equipping sensors of other types for precise measurement of other parameters.

This invention relates to a gene-chip for plant virus detection, particularly it relates to a method, by utilizing gene-chip, to detct samples if it contains plant virus. It is used for novel nucleic acid primer of PCR, or, for detecting PCR product probe. This method can be used for detecting simultaneously determinand sample with 2-122 kinds of virus, sich as: AIMV, CMV, PVA, PVX, PVY, TMY, BBTV, LSV, SqMV, CEVD, ASGV, PXP, PVS, LMoV, DsMV, ZYMV, WMV, CRSV, CCCVd, PNRSV, SBMV, TRSV and viroid PSTVd etc.

The invention discloses a method and a device for determining the flatness of a surface. The method comprises the following steps: acquiring spatial position information of multiple collection points on a to-be-measured surface in turn, wherein the spatial position information includes horizontal coordinate data, longitudinal coordinate data and height data of the collection points; determining a datum plane, wherein the datum plane is a plane formed through fitting according to the spatial position information of the multiple collection points; and determining the height error between each collection point and the datum plane, and calculating the flatness of the to-be-measured surface according to the height error. As the datum plane matching the collection points most is determined through fitting, error generated when an actual plane is used as a datum plane is avoided, potential error caused by manual intervention is eliminated, and the precision of flatness detection is improved greatly. Moreover, the method can run automatically, improves the repeatability and reproducibility of detection, and is suitable for automatic production lines.

The invention relates to a one-tube magnetic bead method for extraction of virus nucleic acid for fluorescent quantitative detection, which belongs to the technical field of biological nucleic acid application and includes: firstly, preparing lysis solution, adding the lysis solution and magnetic beads into a tube strip, adding a sample of virus nucleic acid into the tube strip, and mixing well to lead the magnetic beads to fully absorb the virus nucleic acid; secondly, placing magnetic bead mixed liquor on a magnetic carrier, and allowing the liquor to stand for seconds to separate the magnetic beads and solution; and thirdly, rinsing for protein removal and desalination, and drying in air at room temperature. The method is simple and quick, less apt to cause pollution and high in flexibility. By the method, extracting efficiency is high, nucleic acid purity is high, testing repeatability is good, compatibility to amplification reagents is high, production cost is reduced, operation accuracy is improved, and works such as clinical diagnosis, medicolegal identification and the like are facilitated greatly.

The invention discloses FISH probe groups for detecting a novel coronavirus SARS-CoV-2. The probe groups comprise at least two fluorescent probe groups among a first fluorescent probe group taking anS gene as a target, a second fluorescent probe group taking an E gene as a target, a third fluorescent probe group taking an M gene as a target, a fourth fluorescent probe group taking an ORF 3a geneas a target, a fifth fluorescent probe group taking an N gene as a target, and a sixth fluorescent probe group taking an ORF 1ab gene as a target. In the at least two fluorescence probe groups, fluorescence molecules labeled by at least one fluorescence probe group have a fluorescence emission spectrum different from that of the other fluorescence probe groups. The FISH probe groups have high specificity and sensitivity, can realize positioning detection of SARS-CoV-2 in a to-be-detected sample, obtain the distribution and relative quantification conditions of the SARS-CoV-2, are an effectivesupplement for novel coronavirus nucleic acid detection, and have important clinical application value.

The invention provides a polymer surface charge and trap level characteristic measuring device and method and relates to the technical field of surface charge measurement of solid insulating materials. The measuring device comprises a high-voltage power source, a protection resistor, a low-voltage power source, a sealed air chamber, a high-voltage charging device and a potential measuring device.The measuring method comprises the steps: firstly, the high-voltage power source is connected with a needle electrode through the protection resistor, a gate electrode is connected with the low-voltage power source, charges are injected into a test sample slice of the insulating material, a power supply is cut off after charging finishes, a probe moving guiding rod is utilized to move a probe to be above a test sample, rotation of a rotary connection guiding rod is matched to drive the test sample to achieve full-plane potential measurement on the test sample, a change curve of the potential along with resolution time is drawn, and a mathematic formula is utilized to calculate and analyze charge trap level distribution of a gas-solid interface. The measuring device and method disclosed bythe invention can be suitable for surface charge measurement and trap level distribution researches of gas-solid interfaces under different voltage form types, different solid insulating materials anddifferent insulating gases; a measuring range is wide.

The invention discloses a multi-mode laser-induced breakdown spectroscopy device. The multi-mode laser-induced breakdown spectroscopy device comprises three operating modes which are collineation, reheating and pre-ablation; in the collineation operating mode, an optical path climbing system is arranged on the optical path of a double pulse solid laser, and is used for raising the optical path of 1065nm/532nm coaxially output laser, so as to ensure that laser is focused to the surface of a sample from the part above a sample table to produce plasma; in the reheating and pre-ablation operating modes, first laser of the double pulse solid laser enters the optical path climbing system, and the optical path of the first laser is raised, so that the first laser is focused to the surface of the sample from the part above the sample table to produce plasma; a delay generator controls the triggering timing sequence of the first laser and second laser; the second laser of the double pulse solid laser is directly focused to the plasma; the multi-mode laser-induced breakdown spectroscopy device further comprises a signal gathering and processing system which displays distribution information of elements on the surface of the sample according to characteristic spectral lines emitted by the plasma when the plasma is cooled and space information of the first laser when the first laser beats the sample.

The invention discloses an automobile sideslip detector and a method for detecting sideslip. The automobile sideslip detector consists of a mechanical part and an electric part, wherein chain wheels of the mechanical part are fixed on a chain wheel shaft, and a plane link plate and two chain wheels form chain drive connection; a base plate is arranged on a sliding plate, the sliding plate is connected with a plate type linear guide rail through a ball bearing, and the sliding plate can reciprocate on the plate type guide rail transversely; a left-shift sensor and a right-shift sensor of the electric part are connected with an amplifier respectively, and the amplifier is connected with a microprocessor through an A/D converter; a rotary encoder is connected with the microprocessor through a counting circuit; and a rotating shaft of the rotary encoder and the chain wheel shaft are arranged coaxially, and the fixed parts of the left-shift sensor and the right-shift sensor are arranged on the plate type linear guide rail. The sideslip detector amplifies the detection running distance X of a detected automobile so that the detection is more accurate, and can measure sideslip values at different speeds and different loads for accurately matching suspension fork positional parameters of a front steering wheel.

The invention discloses a flexible surface enhanced Raman detection substrate, a preparation method and a preparation system thereof, which comprises a film substrate layer, a cured resin layer, a plurality of convex structures and a gold thin film layer, wherein the cured resin layer is attached to the surface of the film substrate layer and is doped with nano silver particles; the nano convex structures are distributed on the surface of the cured resin layer and made of resin doped with nano silver particles, and the nano silver particles are distributed on the surfaces of the convex structures; and the gold thin film layer is attached to the surfaces of the cured resin layer and the convex structure. The substrate has better enhancement effect of Raman scattering signals and higher detection precision and accuracy.

The invention discloses a reagent for detecting antithrombin activity, including reagent 1 and reagent 2, wherein: reagent 1 is a chromogenic substrate of FXa, and reagent 2 is a diluent containing FXa and heparin; wherein the chromogenic substrate of FXa is selected from From the following: CH ₃ SO ₂ ‑D‑Leu‑Gly‑Arg‑p‑nitroanilide·AcOH; CH ₃ OCO‑D‑CHG‑Gly‑Arg‑p‑nitroanilide·AcOH; CH ₃ OCO‑D‑CHA‑Gly‑Arg‑p‑nitroanilide AcOH; Benzoyl‑Ile‑Glu‑Gly‑Arg‑p‑nitroanilide HCl; Suc‑Ile‑Glu(γ‑Piperidyl)‑Gly‑Arg‑p‑nitroanilide HCl; N-α-Z-D-Arg-Gly-Arg-p-nitroanilide 2HCl; Boc-D-Arg-Gly-Arg-p-nitroanilide 2HCl; Acetyl-D-Arg-Gly-Arg-p ‑nitroanilide·2HCl; 4‑Nz‑D‑Arg‑Gly‑Arg‑p‑nitroanilide·2HCl; 4‑Mbs‑D‑Arg‑Gly‑Arg‑p‑nitroanilide·2HCl. The invention has good detection stability and repeatability, high sensitivity and accuracy, can accurately reflect the activity of antithrombin, and can find patients with abnormal antithrombin activity in the body, thereby providing a basis for treatment.

The invention, which belongs to the technical field of virus detection, provides an anti-African swine fever P30 protein single-domain antibody and an ELISA kit for detecting the African swine fever virus. The amino acid sequence of the single-domain antibody p30-17 for resisting the African swine fever virus P30 protein is as shown in SEQ ID No. 1. In addition, the invention also relates to an ELISA kit for detecting African swine fever virus based on a double-antibody sandwich method. The ELISA kit comprises an elisa plate coated with a capture antibody being a single-domain antibody p30-17,an enzyme-labeled detection antibody being a single-domain antibody p30-30 of the anti-African swine fever virus p30 protein with an amino acid sequence shown as SEQ ID No.2, a standard positive reference substance, a serum diluent, a substrate developing solution A, a substrate developing solution B, a stop solution and a concentrated washing solution. The kit can realize rapid and accurate detection and has good detection specificity.

The invention relates to a stereovision-based method and a stereovision-based device for detecting a vehicle wheelbase difference and belongs to vehicle performance detection equipment. The method and the device are mainly used to automatically detect the vehicle wheelbase difference on a vehicle comprehensive performance detection line. According to vehicle wheel images captured by four cameras, the detection device identifies the center three-dimensional coordinates of the vehicle wheel images by adopting a computer image processing technology and calculates the vehicle wheelbase difference on the basis of the center three-dimensional coordinates of the vehicle wheels. The system hardware comprises the cameras, an industrial control computer, an opposite-type photoelectric sensor, a revolving platform and a customized calibration target. The method and the device can rapidly and accurately detect the vehicle wheelbase difference. The method has the advantages of unique design idea, excellent linearity, less systematic error, excellent detection precision and repeatability, simple structure and convenient use. The research results have a certain theoretical value and economic value and good prospect of application and popularization.

The invention relates to a method for detecting the content of each group of free polysaccharide in a meningococcus polysaccharide conjugate vaccine finished product and belongs to the technical field of biology. The method comprises the following steps of: during preparation of a detected product sample, carrying out proteolytic enzyme K treatment, wherein the addition amount of the proteolytic enzyme K is 2-8 times the content of the protein in an enzymolysis reaction system; carrying out cold phenol solution treatment, namely separating combined polyose and free polyose in a complex compound by utilizing a sodium acetate saturated cold phenol solution; measuring the content of each group of free polysaccharide in the conjugate vaccine finished product by virtue of the conjunctive use of an immunoelectrophoresis detection technology. The method provided by the invention can be used for eliminating the influence of each group of complex compound carrier protein on relevant factors such as electrophoretic mobility during immunoelectrophoresis, effectively separating the combined polyose from the free polyose in the complex compound and establishing a suitable detection system for each group of free polysaccharide in the conjugate vaccine finished product with more than four groups, and has the characteristics of strong durability, accuracy and high precision, thereby establishing a method for evaluating the quality of the meningococcus polyose conjugate vaccine finished product with more than four groups.

The invention relates to a testing method for puncture resistance capabilities of battery diaphragm. The method includes that a diaphragm sample is put between two plate electrodes to form a test piece, the surface of at least one plate electrode is provided with burrs and comprises a plurality of hard conductive particles protruding out of the surface, the diaphragm sample is correspondingly placed on the burred surface in a contact mode, and the two plate electrodes are electrically connected to two sides of an insulated resistance tester; the test piece is put in an environment with changeable and controllable temperatures, and the pressure value of forward extrusion of the plate electrodes is increased through external force; when the insulated resistance tester detects short circuit of the plate electrodes of the test piece, the pressure value, used for representing the puncture resistance capability of the diaphragm sample, at the moment is recorded. Preferably, two dry abrasivepapers with hard conductive alloy particles on the surfaces are substituted for the two plate electrodes. The invention further relates to a combined testing device applied to tests of the puncture resistance capabilities of the battery diaphragm.

The invention discloses an electrochemical detection chip. The electrochemical detection chip comprises an electrode layer, wherein a working electrode of the electrode layer comprises a conducting layer, a nanomaterial layer and a blood clotting reaction layer which are sequentially laminated, wherein the blood clotting reaction layer is used for producing an electrical signal for detecting prothrombin time; the nanomaterial layer is used for transferring and amplifying the electrical signal. By using high conductivity, large specific area, good biocompatibility and the like of the nanomaterial layer, amplification and enhancement of the electrical signal in the detection process of the prothrombin time are realized so as to improve the sensitivity of chip detection and shorten the detection time. The invention discloses an electrochemical sensor. The electrochemical sensor comprises the electrochemical detection chip. The invention discloses a preparation method of the electrochemical sensor. The preparation method is suitable for preparing the electrochemical sensor with high sensitivity and accurate detection results.

The invention discloses a canine parvovirus colloidal gold immunochromatography test strip and a preparation method. The test strip comprises a sample absorbing area, a gold label probe area, an immobilized antibody area, a water absorbing area and a bottom plate, wherein the sample absorbing area, the gold label probe area, the immobilized antibody area and the water absorbing area are sequentially paved on the bottom plate and partially overlapped each other; the gold label probe area is coated with a colloidal gold labeled anti-canine parvovirus monoclonal antibody F1; the immobilized antibody area is sequentially provided with a test line and a control line, the test line is coated with an anti-canine parvovirus monoclonal antibody B6, and the control line is coated goat anti-mouse IgG (immunoglobulin G). According to the preparation method, a glass fiber membrane, a polyester membrane coated with the anti-canine parvovirus monoclonal antibody F1, a nitrocellulose membrane coated with the test line and the control line, and water absorbing filter paper are assembled onto a polyethylene plate to obtain the canine parvovirus colloidal gold immunochromatography test strip. The test strip is fast to test, high in accuracy rate, high in specificity, and simple and convenient to carry and operate.