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190results about How to "Good detection repeatability" patented technology

Screw rotor type surface profile error measurement instrument

ActiveCN102538700AAvoiding Probe Radius Compensation ProblemsHigh measurement accuracyUsing optical meansEngineeringHeadstock
The invention discloses a screw rotor type surface profile error measurement instrument. The screw rotor type surface profile error measurement instrument is characterized in that: a measured rotor is supported between a headstock center and a tailstock center, and the measured screw rotor is driven by rotary working platforms to rotate; and a movable working platform is arranged, a measurement unit is arranged on the movable working platform, a long grating sensor for detecting the position of the movable working platform is arranged in the measurement unit, and a laser displacement sensor is fixedly arranged on an electric rotary disk. According to the screw rotor type surface profile error measurement instrument disclosed by the invention, the original measurement data on the screw rotor type surface feature is acquired under the motion coordination of two rotary working platforms and a one-dimensional translation platform by using the laser displacement sensor, and the corresponding data treatment and coordinate conversion are performed, so that the screw rotor type surface profile error data can be obtained and the error assessment result is given; and the screw rotor type surface profile error measurement instrument is simple in structure and good in processing and assembling performance, and is capable of realizing the surface profile error measurement on various screw surfaces and compatibly equipping sensors of other types for precise measurement of other parameters.
Owner:HEFEI UNIV OF TECH

Simulative-target metabonomics analytic method based on combination of liquid chromatography and mass spectrum

The invention provides a simulative-target metabonomics analytic method based on combination of liquid chromatography and mass spectrum. The method includes following steps: respectively manufacturing to-be-analyzed samples into merged samples according to groups; automatically collecting secondary mass spectrums of metabolites in each merged samples through data of UHPLS / Q-TOF MS with dependence on a collecting mode; extracting a retaining time and information of parent ions and daughter ions of the metabolites by qualitative analytic software; screening out characteristic ion pair information of the metabolites according to daughter ion response intensity; summing the characteristic ion pair information of each merged samples; and scanning the obtained characteristic ion pair of the metabolites by UHPLC / QQQ MS through a dynamic multi-reaction monitoring mode in an actual sample for obtaining a corresponding spectrogram; and performing integration through quantitative analytic software to obtain the metabolites in the to-be-analyzed samples and content information thereof.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Method of detecting residue of small-molecule substance harmful to human body and a special kit

The invention discloses a method of detecting the residue of small-molecule substances harmful to the human body and a special kit. The special kit comprises a non-transparent micro-porous plate and a light-emitting compound, wherein each hole of the non-transparent micro-porous plate is filled with a coat antigen which is simultaneously coated with three kinds of small-molecule substances. The invention makes full use of the multi-color marking function of QDs, establishes a novel kit for simple and rapid detection of the residue and a method thereof, and realizes the multi-color marking through indirect marking of polyclonal antibodies and monoclonal antibodies in the veterinary drug by coupling the QDs with different particle sizes and targets with functional groups (such as an amino) with specific surfaces. The method comprises: obtaining quantum dots with different fluorescent characteristics through separation and purification, namely, multi-color antibody markers, using the multi-color antibody markers as fluorescent probes, and establishing a reaction system for synchronous analysis of various antigen components of different kinds, thereby realizing the synchronous detection of multiple kinds of residues of the veterinary drug in animal food. Moreover, the method has the advantages of simple operation, high fluorescence intensity and long stabilization time.
Owner:CHINA AGRI UNIV

Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof

The invention relates to a particle-enhanced turbidimetric immune assay kit for detecting adiponectin and a preparation method thereof, in particular to a detection method of a turbidimetric immune assay. The particle-enhanced turbidimetric immune assay kit for detecting the adiponectin comprises a kit body, an application liquid bottle and a latex suspension bottle coating an antiviral adiponectin monoclonal antibody, wherein the application liquid bottle is filled with application liquid; the application liquid comprises a surfactant, a preservative, a macromolecular accelerator, sodium chloride and a buffering agent; the latex suspension bottle coating the antiviral adiponectin monoclonal antibody is filled with a latex suspension coating the antiviral adiponectin monoclonal antibody; and the latex suspension coating the antiviral adiponectin monoclonal antibody comprises latex coating the antiviral adiponectin monoclonal antibody, the surfactant, a stabilizer and the buffering agent. The preparation method comprises the following steps of: preparing a latex antibody; preparing the latex suspension coating the antiviral adiponectin monoclonal antibody; preparing the applicationliquid; and finally preparing adiponectin series standard products.
Owner:ANHUI IPROCOM BIOTECH CO LTD

Gene chip for detecting plant virus

This invention relates to a gene-chip for plant virus detection, particularly it relates to a method, by utilizing gene-chip, to detct samples if it contains plant virus. It is used for novel nucleic acid primer of PCR, or, for detecting PCR product probe. This method can be used for detecting simultaneously determinand sample with 2-122 kinds of virus, sich as: AIMV, CMV, PVA, PVX, PVY, TMY, BBTV, LSV, SqMV, CEVD, ASGV, PXP, PVS, LMoV, DsMV, ZYMV, WMV, CRSV, CCCVd, PNRSV, SBMV, TRSV and viroid PSTVd etc.
Owner:北京金长河科技发展有限公司

Method for representing cohesive force between diaphragm and pole piece of lithium battery coated with polymer

The invention discloses a method for representing a cohesive force between a diaphragm and a pole piece of a lithium battery coated with polymer. The method comprises the following steps: firstly, overlapping the diaphragm coated with a coating layer with the pole piece; adopting a roller press for hotly gluing the diagram with the pole piece, wherein one side coated with the coating layer is faced to the pole piece; cutting a sample for the adhered diaphragm and pole piece coated with the polymer, thereby obtaining a bar-shaped sample; using a universal tensile testing machine for stripping the bar-shaped sample and recording a force value at a stripping moment; calculating the bonding strength between the diaphragm and pole piece coated with the polymer. The method disclosed by the invention has the advantages of being simple and convenient in operation, simple in equipment, high in detecting repeatability and high in working efficiency; the bonding strength value after hot pressing between the diaphragm and the pole piece of the lithium battery coated with polymer can be accurately and conveniently obtained; the method is suitable for the detection process for the bonding strength value after hot pressing between the diaphragm and the pole piece of the lithium battery coated with polymer in different sizes; the application scope is wide.
Owner:SHENZHEN SENIOR TECH MATERIAL

Manufacturing method of carbon nanotube gas sensor based on corona discharge

The invention provides a manufacturing method of a carbon nanotube gas sensor based on corona discharge and is characterized by providing a novel carbon nanotube ion type gas sensor by using the unique physical structure of the carbon nanotube and the tip emission effect based on the gas corona discharge principle. The sensor is formed by aligned carbon nanotube arrays grown on an anodic aluminium oxide template. The carbon nanotubes and the electrodes are integrated, thus simplifying the structure of the device and the process and ensuring convenient control and measurement. Compared with the prior art, the gas sensor has the advantages of simple structure, lower cost, high sensitivity, good detection repeatability, low energy consumption, small occupied space and convenient use. The gas sensor requires lower direct current voltage, thus reducing the danger level of operation and being expected to realize the portable gas monitoring device.
Owner:ZHEJIANG UNIV

FXa activity detection reagent, preparation method and application of FXa activity detection reagent

The invention discloses a detection method for heparin content. The detection method comprises the steps of mixing a sample to be tested with an FXa activity detection reagent by a developing substrate method to obtain a mixture, incubating the mixture, and detecting the signal intensity of a developing substrate; and finally calculating signal intensity and comparing the calculated signal intensity with a standard curve to obtain the heparin content of the sample to be tested, wherein the developing substrate is a developing substrate of an activated factor X (FXa) and is selected from any one of the following substrates of Benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide.HCl, CH3O-CO-D-CHA-Gly-Arg-p-nitroanilide.AcOH, Acetyl-D-Arg-Gly-Arg-p-nitroanilide.2HCl and 4-Nz-D-Arg-Gly-Arg-p-nitroanilide.2HCl. The detection method disclosed by the invention can be used for detecting general non-graded heparin, low-molecular-weight heparin and fondaparinux; furthermore, according to the detection method, the detection stability and the repetitiveness are high, the heparin content can be reflected accurately, the sensitivity and the accuracy are extremely high, and the optimal dosage range of the heparin can be found out quickly, so that a patient does not need to be detected for multiple times. Moreover, the detection method can be also used on an automatic instrument such as a coagulation analyzer or a biochemical analyzer, so that automation is realized, and clinical popularization and application can be assisted; the detection method has the characteristics of simplicity in operation, high sensitivity and high repetitiveness.
Owner:SHANGHAI VASCUTECH DIAGNOSIS CO LTD

One-tube magnetic bead method for extraction of virus nucleic acid for fluorescent quantitative detection

The invention relates to a one-tube magnetic bead method for extraction of virus nucleic acid for fluorescent quantitative detection, which belongs to the technical field of biological nucleic acid application and includes: firstly, preparing lysis solution, adding the lysis solution and magnetic beads into a tube strip, adding a sample of virus nucleic acid into the tube strip, and mixing well to lead the magnetic beads to fully absorb the virus nucleic acid; secondly, placing magnetic bead mixed liquor on a magnetic carrier, and allowing the liquor to stand for seconds to separate the magnetic beads and solution; and thirdly, rinsing for protein removal and desalination, and drying in air at room temperature. The method is simple and quick, less apt to cause pollution and high in flexibility. By the method, extracting efficiency is high, nucleic acid purity is high, testing repeatability is good, compatibility to amplification reagents is high, production cost is reduced, operation accuracy is improved, and works such as clinical diagnosis, medicolegal identification and the like are facilitated greatly.
Owner:TIANGEN BIOTECH BEIJING

FISH probe groups for detecting novel coronavirus SARS-CoV-2 and preparation method and application thereof

ActiveCN111254227AImprove the current situation of insufficient detection capabilitiesEarly detectionMicrobiological testing/measurementMicroorganism based processesFluoProbesNucleic acid detection
The invention discloses FISH probe groups for detecting a novel coronavirus SARS-CoV-2. The probe groups comprise at least two fluorescent probe groups among a first fluorescent probe group taking anS gene as a target, a second fluorescent probe group taking an E gene as a target, a third fluorescent probe group taking an M gene as a target, a fourth fluorescent probe group taking an ORF 3a geneas a target, a fifth fluorescent probe group taking an N gene as a target, and a sixth fluorescent probe group taking an ORF 1ab gene as a target. In the at least two fluorescence probe groups, fluorescence molecules labeled by at least one fluorescence probe group have a fluorescence emission spectrum different from that of the other fluorescence probe groups. The FISH probe groups have high specificity and sensitivity, can realize positioning detection of SARS-CoV-2 in a to-be-detected sample, obtain the distribution and relative quantification conditions of the SARS-CoV-2, are an effectivesupplement for novel coronavirus nucleic acid detection, and have important clinical application value.
Owner:SHANGHAI GENEPHARMA CO LTD

Heparin content detection method

The invention discloses a heparin content detection method. The heparin content detection method comprises the following steps of by a developing substrate method, mixing a sample to be detected and a FXa activity detection reagent, carrying out incubation, detecting signal intensity of the developing substrate, calculating signal intensity and comparing the signal intensity and a standard curve to obtain heparin content of the sample to be detected, wherein the developing substrate is a developing substrate of an activation factor X(FXa) and is selected from Benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide.HCl, CH3O-CO-D-CHA-Gly-Arg-p-nitroanilide.AcOH, Acetyl-D-Arg-Gly-Arg-p-nitroanilide.2HCl, and 4-Nz-D-Arg-Gly-Arg-p-nitroanilide.2HCl. The heparin content detection method can be used for detecting common unclassified heparin, low-molecular weight heparin and fondaparinux, has good detection stability and good repeatability, can accurately show heparin content, has high sensitivity and accuracy, can fast find the optimal dosage scope of heparin and is free of multitime detection on patients. The heparin content detection method can be used for automation apparatuses such as a hemagglutination analyzer or a biochemical analyzer, realizes automation, is conducive to clinical popularization use and has the characteristics of simple operation, high sensitivity and good repeatability.
Owner:SHANGHAI VASCUTECH DIAGNOSIS CO LTD

Polymer surface charge and trap level characteristic measuring device and method

PendingCN108445310AFigure out the transport characteristicsGuiding significance of typical engineering practiceTesting dielectric strengthElectrical measurement instrument detailsPolymeric surfacePotential measurement
The invention provides a polymer surface charge and trap level characteristic measuring device and method and relates to the technical field of surface charge measurement of solid insulating materials. The measuring device comprises a high-voltage power source, a protection resistor, a low-voltage power source, a sealed air chamber, a high-voltage charging device and a potential measuring device.The measuring method comprises the steps: firstly, the high-voltage power source is connected with a needle electrode through the protection resistor, a gate electrode is connected with the low-voltage power source, charges are injected into a test sample slice of the insulating material, a power supply is cut off after charging finishes, a probe moving guiding rod is utilized to move a probe to be above a test sample, rotation of a rotary connection guiding rod is matched to drive the test sample to achieve full-plane potential measurement on the test sample, a change curve of the potential along with resolution time is drawn, and a mathematic formula is utilized to calculate and analyze charge trap level distribution of a gas-solid interface. The measuring device and method disclosed bythe invention can be suitable for surface charge measurement and trap level distribution researches of gas-solid interfaces under different voltage form types, different solid insulating materials anddifferent insulating gases; a measuring range is wide.
Owner:SHENYANG POLYTECHNIC UNIV

Colloidal gold detection card for rapidly detecting melamine and preparing method thereof

The invention provides a colloidal gold cassette for rapid detection of melamine, the colloidal gold cassette has rapid detection, convenient carry and simple operation, the invention also provides a preparation method of the cassette, the preparation process thereof is simple, and the cost is low. The colloidal gold cassette comprises a test strip, the test strip is packaged in a shell body of the cassette, a sample adding hole and an observation hole are arranged on the shell body of the cassette, the test strip comprises a bottom plate, the colloidal gold cassette is characterized in that water absorbent paper, a nitrocellulose membrane, a gold-labeled pad and a sample pad are sequentially arranged on the bottom plate of the test strip, the water absorbent paper and the gold-labeled pad are respectively arranged at the two ends of the nitrocellulose membrane, small sections of the water absorbent paper and the gold-labeled pad are respectively overlapped on the nitrocellulose membrane at the junctions at the two ends of the nitrocellulose membrane, the sample pad is arranged at the other end of the gold-labeled pad, a small section of the sample pad is overlapped on the gold-labeled pad; a gold-labeled anti-melamine monoclonal antibody is sprayed on the gold-labeled pad; a detection line with the material of a melamine-ovalbumin coupled conjugate and a quality control line with the material of goat anti-mouse IgG are sequentially sprayed on the nitrocellulose membrane.
Owner:江苏省苏微微生物研究有限公司

Multi-mode laser-induced breakdown spectroscopy device

The invention discloses a multi-mode laser-induced breakdown spectroscopy device. The multi-mode laser-induced breakdown spectroscopy device comprises three operating modes which are collineation, reheating and pre-ablation; in the collineation operating mode, an optical path climbing system is arranged on the optical path of a double pulse solid laser, and is used for raising the optical path of 1065nm / 532nm coaxially output laser, so as to ensure that laser is focused to the surface of a sample from the part above a sample table to produce plasma; in the reheating and pre-ablation operating modes, first laser of the double pulse solid laser enters the optical path climbing system, and the optical path of the first laser is raised, so that the first laser is focused to the surface of the sample from the part above the sample table to produce plasma; a delay generator controls the triggering timing sequence of the first laser and second laser; the second laser of the double pulse solid laser is directly focused to the plasma; the multi-mode laser-induced breakdown spectroscopy device further comprises a signal gathering and processing system which displays distribution information of elements on the surface of the sample according to characteristic spectral lines emitted by the plasma when the plasma is cooled and space information of the first laser when the first laser beats the sample.
Owner:ZHEJIANG UNIV

Precision spherical hinge clearance-measuring instrument and measurement method

The invention discloses a precision spherical hinge clearance-measuring instrument and a measurement method. The precision spherical hinge clearance-measuring instrument is characterized in that the precision spherical hinge clearance-measuring instrument is provided with a horizontal base platform, a rectangular outer frame, an inverted U-shaped inner frame, a loading cylinder, an adapter disk and an inductive displacement sensor. Under the cooperation of the motion of the rotatable rectangular outer frame and the rotatable inverted U-shaped inner frame which are perpendicularly crossed by each other, the precision spherical hinge clearance-measuring instrument can acquire the raw clearance error measurement data of the assembling clearance of a spherical hinge, and can obtain the model and rule of the spherical hinge clearance error by means of subsequent corresponding data processing; the clearance error of the spherical hinge within a three-dimensional space can be efficiently, automatically and accurately measured, and the invention can promote the progress of the parallel mechanism error modeling and correction technique.
Owner:HEFEI UNIV OF TECH

Magnetic bead time resolution fluorescence immunoassay quantitative determination CK-MB (creatine kinase-MB) kit

The invention discloses a magnetic bead time resolution fluorescence immunoassay quantitative determination CK-MB kit. The CK-MB kit comprises an immunomagnetic bead coating a CK-MB monoclonal antibody, a CK-MB standardized product solution, a europium-marked CK-MB monoclonal antibody solution, washing liquid and enhancement liquid. The immunomagnetic bead coating the CK-MB monoclonal antibody isa covalent conjugate of a superpara magnetic bead modified by a functional group and with the diameter being 1-3 microns and the CK-MB monoclonal antibody. The kit has the high sensibility, the sensibility of CK-MB is 1ng / mL, and a blood serum (plasma) does not need to be diluted; the determination time is short, and a report can be resulted within 30 minutes; the demanding amount of the sample isless, and only 50 microliters are needed for one-time sample loading; and the kit is equipped with a full-automatic time resolution immune analysis meter, operation is easy, no artificial error exists, and labor is saved. The kit reasonably utilizes the space of a reagent strip, the structure of the reagent strip is more compact, the reagent strip can be transported more easily, and used conveniently, the operation is simple, and the stability is good.
Owner:GUANGZHOU BIOKEY HEALTH TECH CO LTD

Automobile sideslip detector and method for detecting sideslip

The invention discloses an automobile sideslip detector and a method for detecting sideslip. The automobile sideslip detector consists of a mechanical part and an electric part, wherein chain wheels of the mechanical part are fixed on a chain wheel shaft, and a plane link plate and two chain wheels form chain drive connection; a base plate is arranged on a sliding plate, the sliding plate is connected with a plate type linear guide rail through a ball bearing, and the sliding plate can reciprocate on the plate type guide rail transversely; a left-shift sensor and a right-shift sensor of the electric part are connected with an amplifier respectively, and the amplifier is connected with a microprocessor through an A / D converter; a rotary encoder is connected with the microprocessor through a counting circuit; and a rotating shaft of the rotary encoder and the chain wheel shaft are arranged coaxially, and the fixed parts of the left-shift sensor and the right-shift sensor are arranged on the plate type linear guide rail. The sideslip detector amplifies the detection running distance X of a detected automobile so that the detection is more accurate, and can measure sideslip values at different speeds and different loads for accurately matching suspension fork positional parameters of a front steering wheel.
Owner:SOUTH CHINA UNIV OF TECH

Flexible surface enhanced Raman detection substrate and preparation method and preparation system thereof

The invention discloses a flexible surface enhanced Raman detection substrate, a preparation method and a preparation system thereof, which comprises a film substrate layer, a cured resin layer, a plurality of convex structures and a gold thin film layer, wherein the cured resin layer is attached to the surface of the film substrate layer and is doped with nano silver particles; the nano convex structures are distributed on the surface of the cured resin layer and made of resin doped with nano silver particles, and the nano silver particles are distributed on the surfaces of the convex structures; and the gold thin film layer is attached to the surfaces of the cured resin layer and the convex structure. The substrate has better enhancement effect of Raman scattering signals and higher detection precision and accuracy.
Owner:SHANDONG UNIV

Antithrombin activity assay reagent and preparation method and application thereof

The invention discloses a reagent for detecting antithrombin activity, including reagent 1 and reagent 2, wherein: reagent 1 is a chromogenic substrate of FXa, and reagent 2 is a diluent containing FXa and heparin; wherein the chromogenic substrate of FXa is selected from From the following: CH 3 SO 2 ‑D‑Leu‑Gly‑Arg‑p‑nitroanilide·AcOH; CH 3 OCO‑D‑CHG‑Gly‑Arg‑p‑nitroanilide·AcOH; CH 3 OCO‑D‑CHA‑Gly‑Arg‑p‑nitroanilide AcOH; Benzoyl‑Ile‑Glu‑Gly‑Arg‑p‑nitroanilide HCl; Suc‑Ile‑Glu(γ‑Piperidyl)‑Gly‑Arg‑p‑nitroanilide HCl; N-α-Z-D-Arg-Gly-Arg-p-nitroanilide 2HCl; Boc-D-Arg-Gly-Arg-p-nitroanilide 2HCl; Acetyl-D-Arg-Gly-Arg-p ‑nitroanilide·2HCl; 4‑Nz‑D‑Arg‑Gly‑Arg‑p‑nitroanilide·2HCl; 4‑Mbs‑D‑Arg‑Gly‑Arg‑p‑nitroanilide·2HCl. The invention has good detection stability and repeatability, high sensitivity and accuracy, can accurately reflect the activity of antithrombin, and can find patients with abnormal antithrombin activity in the body, thereby providing a basis for treatment.
Owner:SHANGHAI VASCUTECH DIAGNOSIS CO LTD

Anti-African swine fever P30 protein single-domain antibody and ELISA kit for detecting African swine fever virus

The invention, which belongs to the technical field of virus detection, provides an anti-African swine fever P30 protein single-domain antibody and an ELISA kit for detecting the African swine fever virus. The amino acid sequence of the single-domain antibody p30-17 for resisting the African swine fever virus P30 protein is as shown in SEQ ID No. 1. In addition, the invention also relates to an ELISA kit for detecting African swine fever virus based on a double-antibody sandwich method. The ELISA kit comprises an elisa plate coated with a capture antibody being a single-domain antibody p30-17,an enzyme-labeled detection antibody being a single-domain antibody p30-30 of the anti-African swine fever virus p30 protein with an amino acid sequence shown as SEQ ID No.2, a standard positive reference substance, a serum diluent, a substrate developing solution A, a substrate developing solution B, a stop solution and a concentrated washing solution. The kit can realize rapid and accurate detection and has good detection specificity.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI

Stereovision-based method and stereovision-based device for detecting vehicle wheelbase difference

The invention relates to a stereovision-based method and a stereovision-based device for detecting a vehicle wheelbase difference and belongs to vehicle performance detection equipment. The method and the device are mainly used to automatically detect the vehicle wheelbase difference on a vehicle comprehensive performance detection line. According to vehicle wheel images captured by four cameras, the detection device identifies the center three-dimensional coordinates of the vehicle wheel images by adopting a computer image processing technology and calculates the vehicle wheelbase difference on the basis of the center three-dimensional coordinates of the vehicle wheels. The system hardware comprises the cameras, an industrial control computer, an opposite-type photoelectric sensor, a revolving platform and a customized calibration target. The method and the device can rapidly and accurately detect the vehicle wheelbase difference. The method has the advantages of unique design idea, excellent linearity, less systematic error, excellent detection precision and repeatability, simple structure and convenient use. The research results have a certain theoretical value and economic value and good prospect of application and popularization.
Owner:JILIN UNIV

Small-Abbe-error three-dimensional measurement system

The invention discloses a small-Abbe-error three-dimensional measurement system, which is characterized in that the small-Abbe-error three-dimensional measurement system is arranged in a mechanical transmission unit and comprises an X-direction assembly, a Y-direction assembly and a Z-direction assembly, a Z-direction clamp head used for clamping a sensor is fixedly arranged on a Z-direction slide seat arranged in the Z-direction assembly, and gratings consisting of grating rulers and grating heads respectively comprise an X-direction grating assembly, a Y-direction grating assembly and a Z-direction grating assembly. The small-Abbe-error three-dimensional measurement system has the technical effects of high efficiency, accuracy and simplicity when being applied to the thermal deformation detection, and the Abbe errors can be effectively reduced.
Owner:HEFEI UNIV OF TECH

Method for detecting content of each group of free polysaccharide in meningococcus polysaccharide conjugate vaccine finished product

The invention relates to a method for detecting the content of each group of free polysaccharide in a meningococcus polysaccharide conjugate vaccine finished product and belongs to the technical field of biology. The method comprises the following steps of: during preparation of a detected product sample, carrying out proteolytic enzyme K treatment, wherein the addition amount of the proteolytic enzyme K is 2-8 times the content of the protein in an enzymolysis reaction system; carrying out cold phenol solution treatment, namely separating combined polyose and free polyose in a complex compound by utilizing a sodium acetate saturated cold phenol solution; measuring the content of each group of free polysaccharide in the conjugate vaccine finished product by virtue of the conjunctive use of an immunoelectrophoresis detection technology. The method provided by the invention can be used for eliminating the influence of each group of complex compound carrier protein on relevant factors such as electrophoretic mobility during immunoelectrophoresis, effectively separating the combined polyose from the free polyose in the complex compound and establishing a suitable detection system for each group of free polysaccharide in the conjugate vaccine finished product with more than four groups, and has the characteristics of strong durability, accuracy and high precision, thereby establishing a method for evaluating the quality of the meningococcus polyose conjugate vaccine finished product with more than four groups.
Owner:云南沃森生物技术股份有限公司

Testing method and combined testing device for puncture resistance capabilities of battery diaphragm

ActiveCN108627387ATest the ability to pierceComprehensive evaluation of puncture resistanceStrength propertiesElectricityElectrical resistance and conductance
The invention relates to a testing method for puncture resistance capabilities of battery diaphragm. The method includes that a diaphragm sample is put between two plate electrodes to form a test piece, the surface of at least one plate electrode is provided with burrs and comprises a plurality of hard conductive particles protruding out of the surface, the diaphragm sample is correspondingly placed on the burred surface in a contact mode, and the two plate electrodes are electrically connected to two sides of an insulated resistance tester; the test piece is put in an environment with changeable and controllable temperatures, and the pressure value of forward extrusion of the plate electrodes is increased through external force; when the insulated resistance tester detects short circuit of the plate electrodes of the test piece, the pressure value, used for representing the puncture resistance capability of the diaphragm sample, at the moment is recorded. Preferably, two dry abrasivepapers with hard conductive alloy particles on the surfaces are substituted for the two plate electrodes. The invention further relates to a combined testing device applied to tests of the puncture resistance capabilities of the battery diaphragm.
Owner:SOUNDON NEW ENERGY TECH CO LTD

Micro-fluidic chip immunoassay kit and detection method thereof

The invention discloses a micro-fluidic chip immunoassay kit and a detection method thereof. The kit comprises a micro-fluidic chip, a wafer cover plate and a wafer bottom plate, wherein the wafer cover plate and the wafer bottom plate are bonded up and down. One or more detection units are arranged in the wafer bottom plate, and each detection unit comprises a liquid storage tank, a mixed reaction tank, a detection tank, a waste liquid tank and a first vent hole which are connected in sequence; the mixed reaction tank is fixedly provided with a fluorescent probe corresponding to a detection drug; goat anti-mouse immune globulin G and corresponding drug antigens are fixed in the detection tank. The invention also discloses a detection method using the kit. The method has the characteristics of high flux, low sample consumption, high detection speed, simplicity and convenience in operation and the like; the method has the advantages of no need of an electrode, a voltage or an external magnetic field, no need of electroosmosis driving, hot air pump driving or optical capture micropump driving, simultaneous detection of various veterinary drug residues in various matrixes, suitableness for on-site rapid screening analysis, and great alleviation of the detection pressure of related detection personnel.
Owner:SOUTH CHINA AGRI UNIV

Filter bag for fibre analysis and manufacturing method thereof

InactiveCN101590346AOvercoming shortcomings in detectionReduce transfer lossGravity filtersCrude fibreCombustion
The invention relates to a filter bag for fibre analysis and a manufacturing method thereof. A polypropylene melt-blown ultrafine fibre material is adopted to manufacture a U-shaped three-dimensional bag with an opening, wherein the middle part of the bag is formed into a space capable of containing a sample; the fibre fineness of the polypropylene melt-blown ultrafine fibre material is less than 4mum, while the filtration efficiency is more than 99 percent, the resistance is less than 7Pa, the thickness is 0.28+ / -0.03mm and the constant area is 55 to 60g / m . The filter bag has the characteristics of acid resistance, alkali resistance, heat resistance (110 DEG C), no generation of ash after combustion, no nitrogen, high filtration efficiency, convenient and firm sealing by a heat sealing machine, and the like; therefore, the filter bag is suitable for determining crude fibre, neutral rinsing fibre and acidic rinsing fibre in feedstuff.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

Dog anti rabies virus antibody colloidal gold immunochromatography assay detection reagent plate and preparation method thereof

This invention relates to one anti-rabies virus antigen gold immune analysis test agent board and its process method, which comprises the following steps: paving glass fiber paper and aqua fortis fiber film on the underlay film of polyethylene board and polychloroethylene underlay film; covering the film onto the test line and comparison line; the test line is added with gold detector ester polymer film; the comparison line side is added with water absorptive pad; the test line is to cover the virus protein. This invention process method is to cover virus protein analysis film by glue gold label rabbit anti-virus IgG multi-clone antigen to process gold detection combination pad.
Owner:WUHAN J H BIO TECH

Electrochemical detection chip and electrochemical sensor, as well as preparation methods and application thereof

The invention discloses an electrochemical detection chip. The electrochemical detection chip comprises an electrode layer, wherein a working electrode of the electrode layer comprises a conducting layer, a nanomaterial layer and a blood clotting reaction layer which are sequentially laminated, wherein the blood clotting reaction layer is used for producing an electrical signal for detecting prothrombin time; the nanomaterial layer is used for transferring and amplifying the electrical signal. By using high conductivity, large specific area, good biocompatibility and the like of the nanomaterial layer, amplification and enhancement of the electrical signal in the detection process of the prothrombin time are realized so as to improve the sensitivity of chip detection and shorten the detection time. The invention discloses an electrochemical sensor. The electrochemical sensor comprises the electrochemical detection chip. The invention discloses a preparation method of the electrochemical sensor. The preparation method is suitable for preparing the electrochemical sensor with high sensitivity and accurate detection results.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI

Canine parvovirus colloidal gold immunochromatography test strip and preparation method

The invention discloses a canine parvovirus colloidal gold immunochromatography test strip and a preparation method. The test strip comprises a sample absorbing area, a gold label probe area, an immobilized antibody area, a water absorbing area and a bottom plate, wherein the sample absorbing area, the gold label probe area, the immobilized antibody area and the water absorbing area are sequentially paved on the bottom plate and partially overlapped each other; the gold label probe area is coated with a colloidal gold labeled anti-canine parvovirus monoclonal antibody F1; the immobilized antibody area is sequentially provided with a test line and a control line, the test line is coated with an anti-canine parvovirus monoclonal antibody B6, and the control line is coated goat anti-mouse IgG (immunoglobulin G). According to the preparation method, a glass fiber membrane, a polyester membrane coated with the anti-canine parvovirus monoclonal antibody F1, a nitrocellulose membrane coated with the test line and the control line, and water absorbing filter paper are assembled onto a polyethylene plate to obtain the canine parvovirus colloidal gold immunochromatography test strip. The test strip is fast to test, high in accuracy rate, high in specificity, and simple and convenient to carry and operate.
Owner:WUHAN CHOPPER BIOLOGY
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