High-sensitivity kit for detecting 5'-nucleotidase

Abstract

The invention relates to the field of an external diagnostic reagent, and particularly relates to a high-sensitivity kit for detecting 5'-nucleotidase. The kit for detecting 5'-nucleotidase comprises a 5'-nucleotidase R1 reagent, a 5'-nucleotidase R2 reagent and a 5'-nucleotidase calibration product, wherein the 5'-nucleotidase R1 reagent comprises a buffer solution, a stabilizing agent, a preservative agent, an enzyme activator, 4-AAP(4-aminoantipyrine), 18-crown ether-6 and UAO (Uricase), PNP (Purine Nucleoside Phosphorylase), XOD (Xanthine Oxidase) and POD (Peroxidase) reaction enzyme systems; the 5'-nucleotidase R2 reagent comprises a buffer solution, a stabilizing agent, a preservative agent, PO3- and a Trinder developing system; and the 5'-nucleotidase calibration product comprises a buffer solution, a stabilizing agent, a preservative agent and 5'-nucleotidase. The detection kit provided by the invention is enhanced in detection sensitivity by 40% compared with a 'PNP-XOD-POD' enzyme system, and has good application prospects.

Description

technical field [0001] The invention relates to the field of external diagnostic reagents, in particular to a kit for detecting 5'-nucleotidase with high sensitivity. Background technique [0002] 5'-Nucleotidase (5'-Nucleotidase, 5'-NT) is widely distributed in liver, gallbladder, pancreas, intestine, heart, brain, lung, kidney, pituitary, thyroid, prostate and other organs and tissues, located in On the cell membrane, it mainly exists in the bile canaliculi and sinusoidal spaces in the liver. It is an enzyme that catalyzes the hydrolysis of nucleoside-5'-monophosphate to generate nucleoside and inorganic phosphate. Human serum 5'-nucleotidases all come from the cell membrane and are extracellular 5'-nucleotidases with an activity level of 3-12 μmol / L phosphate. 5'-nucleotidase as a glycoprotein. Composed of two subunits with a molecular weight of 70,000, it is a dimer with a hydrophobic region structure. Glycosyl-phosphatidylinositol (GPI) is anchored on the outer surf...

AI Technical Summary

Problems solved by technology

However, during the determination of this method, PO 3- and Mg 2+ (or Mn 2+ ) of the common participation, because many kits can not solve the PO well 3- and Mg 2+ (or Mn 2+ ) to generate precipitation, making the determination of the PO of the reagent 3- and Mg 2+ (or Mn 2+ ) concentration is lower than the optimal concentration required for the reaction, and the interference of ALP to 5'-IMP cannot be completely eliminated, resulting in low sensitivity and poor repeatability, which brings difficulties to the further clinical application of this method

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