36 results about "Radial cartilage" patented technology

The invention discloses an osteochondral integrated repairing gradient hybrid support material and a preparation method thereof. The hybrid support material successively consists of a cartilage layer,a cartilage calcification layer and a calcification lower bone layer from top to bottom, wherein all layers are closely combined into a whole body by virtue of a 3D printing technology. The preparation method comprises the following steps: (1) separately preparing hybrid gel of each layer; (2) establishing an osteochondral integrated support model, and preparing a pre-gel support by virtue of a 3D printing technology; and (3) preparing the osteochondral integrated repairing gradient support by adopting a freeze drying technology. The integrated support prepared by the invention simultaneouslyhas a material gradient and a structure gradient, the cartilage layer, and the bone layer are closely connected into a whole body by virtue of the cartilage calcification layer, so that an effect forisolating the cell migration between the two layers can be realized; and by virtue of the 3D printing technology, the individualized tissue engineering support can be rapidly and precisely prepared according to the requirements of different patients, and the integrated construction of a complicated appearance and internal micro-structure of the support can be realized, and the application potential is great.

The invention discloses a method for culturing an artificial cartilage with different curved surfaces or a bone cartilage under the action of rolling load and sliding load. The method comprises an incubator which contains O2 and CO2 and can perform temperature adjustment and cell culture, perfusion and other conditions which are used for keeping culture solution fresh, and active factors which are added to the culture solution. The method is characterized in that culture blanks with different curved surfaces are fixed on the bottom plate of a culture room; during the culturing process, a culture is subjected to the effect of the rolling load and the sliding load simultaneously, and the culture is cultured into cartilage tissues or bone cartilage complex tissues. The method has the advantages that under the effect of the rolling load and the sliding load, the loading way of the method is closer to the mechanical environment of the growth and the development of the cartilage tissues; and under the loading condition, the method is favor of the growth and the development of cells and tissues. An experiment shows that mucopolysaccharide secreted by cartilage cells under the effect of the rolling load and the sliding load is higher than the mucopolysaccharide secreted by the cartilage cells only under the effect of the rolling load. After being cultured, the cultures with different shapes can be taken as implants, and the method of the invention is applied in repairing the defect of the cartilage in different parts.

The invention relates to a method for constructing regenerated nerve vascularized bones, cartilages, joints or body surface organs by a prefabricated female die bracket. Firstly, the female die bracket of a defected tissue or organ is printed in a three-dimension manner by using a computer assisted design/manufacturing technology; then, a surface of a periosteum and/or perichondrium is formed in a body through an operation, the female die bracket is placed and fixedly sutured on a germinal layer of the autologous periosteum and/or perichondrium to form a closed space; after the bones, cartilages, joints or body surface organs whose male die shape corresponds to a female die are regenerated in the space constructed by the female die and the germinal layer of the surface of the periosteum and/or perichondrium, a newly regenerated tissue is taken out, the female die bracket is removed, and the tissue is transplanted to the position of the defected tissue or organ to reconstruct. The newly generated tissue or organ prepared through autologous prefabrication has rich new vessels and nerves, does not contain any exogenous materials participating in tissue regeneration, is high in safety, accurate in shape at the same time, and quite similar to the normal tissue or organ.

The present disclosure relates to a three-dimensionally (e.g., 3D) printed, surgically implantable tissue engineering scaffolds for promoting bone, vascular, and/or cartilage regeneration at osteochondral regions and a method for manufacturing the 3D printed surgically implantable tissue engineering scaffold. The 3D printed surgically implantable tissue engineering scaffold may be fabricated at least in part from a thermoplastic polyurethane (e.g., nTPU) composite via a rapid prototyping machine. In some cases, the three-dimensional shape of the fabricated tissue engineering scaffold may correspond to a three-dimensional shape of a tissue defect of a patient.

The invention discloses a preparation method of a natural tissue-derived epiphyseal cartilage combined bone acellular material, and belongs to the technical field of biological materials for tissue or organ repair and regeneration. The preparation method comprises the following steps: rinsing and repeatedly freezing and thawing epiphyseal cartilage combined bone at the distal femur of a young large white pig, and treating the epiphyseal cartilage combined bone with a PBS buffer solution containing TritonX-100, a PBS buffer solution containing SLES, a PBS buffer solution containing HTHOPS, a PBS buffer solution containing DNaseI and normal saline to obtain the decellularized epiphyseal cartilage combined bone material. After thorough decellularization treatment is carried out on the epiphyseal and cartilage combined bone material, the completeness of the epiphyseal and cartilage combined bone ECM is reserved to the maximum extent while cells with immunogenicity and cell content components are completely removed, and the epiphyseal and cartilage combined bone ECM has the capability of repairing osteochondral defects and can be used for treating orthopedic diseases caused by osteochondral defects.

Described herein are hydrogels attached to a base with the strength and fatigue comparable to that of cartilage on bone and methods of forming them. The methods and apparatuses described herein may achieve an attachment strength between a hydrogel and a substrate equivalent to the osteochondral junction. In some examples the hydrogel may be a triple-network hydrogel (such as BC-PVA-PAMPS) that is attached to a porous substrate (e.g., a titanium base) with the shear strength and fatigue strength equivalent to that of the osteochondral junction.

The invention relates to a method of producing an elastin-reduced cartilage scaffold containing channels and/or lacunae, the method comprising the steps of providing an elastic cartilage sample and reducing elastin from said cartilage sample to produce said channels and/or lacunae. The invention further relates to an elastin-reduced cartilage scaffold. Said elastin-reduced cartilage scaffold can be used in a method of implantation in vivo, for repairment of cartilage, repairment of osteochondral defects and repairment of bone defects.

The invention discloses a preparation method of a personalized biphase condylar scaffold. The preparation method comprises the following steps of separating a mandible digital model by collecting data; selecting data on the opposite side of a lesion area in the mandible digital model, mapping the data to the lesion area in a mirror symmetry manner to obtain a condylar head and neck prosthesis model, and designing and printing a bone phase scaffold according to a condylar tissue structure form; preparing a cartilage phase scaffold; and spraying a compact layer on the surface of the head of the bone phase scaffold, connecting the cartilage phase scaffold with the compact layer through a dissolution-adhesion process to form a compact layer bone-cartilage composite scaffold, constructing a condylar biphase composite scaffold, and after a position retaining handle is designed on the condylar biphase composite scaffold, constructing the personalized biphase bionic condylar scaffold. The problems that the condylar form cannot be recovered and complications of a bone supply area exist in autologous bone graft temporomandibular joint reconstruction are solved, and the problems that the material mechanical property and the degradation period of an existing prosthesis cannot meet clinical requirements are also solved.

The present invention provides implants useful for treating cartilage and/or osteochondral defects that comprise a plurality of scaffolds arranged in a multi-layer stacked configuration, wherein each scaffold comprises a mesh of polymer fibers and wherein the polymer fibers comprise gelatin, a plant-derived protein, e.g., zein protein, or a combination thereof. Methods for repairing a cartilage and/or an osteochondral defect using implants of the invention are also provided.

A method of treating hyaline cartilage of a subject is provided, the method including implanting a first exposed electrode surface in osteochondral tissue of the subject, and implanting a second exposed electrode surface in a body of the subject. Regeneration of the hyaline cartilage is promoted by activating control circuitry to drive the first and the second exposed electrode surfaces to drive nutrients toward the first exposed electrode surface. Other embodiments are also described.