Preparation method and application of tissue engineering biological scaffold
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A biological scaffold and tissue engineering technology, applied in the field of biomedicine, can solve the problems of low biological activity, high content, difficult three-dimensional network structure of tissue engineering scaffolds, etc., achieve high specific surface area, increase biological activity, good three-dimensional porous effect of structure
Pending Publication Date: 2021-11-16
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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However, the bioactive glass nanospheres are all granular products, and their components have a tendency to devitrify. It is difficult to prepare a tissue engineering scaffold with a three-dimensional network structure by sintering, and the SiO 2 High content, low biological activity
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preparation example Construction
[0033] The present invention provides a kind of preparation method of tissue engineering biological scaffold, comprising the following steps:
[0034] Step 1: Prepare bioactive glass nanospheres by sol-gel method, mix a certain amount of distilled water and absolute ethanol, add ammonia water to adjust the pH to alkaline; add ethyl orthosilicate, stir rapidly, and react for 30 minutes; add four Calcium nitrate in water, reacted for 4 hours; 8000 rpm, centrifuged for 10 minutes, collected the reaction product, then separated, washed twice with deionized water and absolute ethanol; dried in an oven at 50°C overnight, and then placed in a muffle furnace at 700°C Sintering and grinding to obtain ordinary biological glass nanospheres;
[0035] Step 2: Prepare the extracellular matrix gel of acellular cartilage by physical pulverization and differential centrifugation; take some fresh cartilage slices, wash them with sterile distilled water, and freeze and thaw them repeatedly; phys...
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[0045] This example discloses a preparation method for constructing a bioscaffold composed of magnesium-containing bioactive glass nanospheres composited with decellularized cartilage extracellular matrix and its use for repairing tissue engineered cartilage. The preparation method includes the following steps:
[0046] 1. Preparation of magnesium-containing bioactive glass nanospheres:
[0047] Step 1. Make a mixed liquid with 12ml tetraethyl orthosilicate (99%) and 48ml alcohol (96% VWR), shake and mix well.
[0048] Step 2. Mix 33ml of absolute ethanol, 18ml of ammonium hydroxide (25%), and 100ml of deionized water to prepare a reaction solution.
[0049] Step 3. Add the mixed solution prepared in step 1 to the reaction solution prepared in step 2, stir with a magnetic stirrer, and react for 30 minutes.
[0050] Step 4. Add a certain amount of calcium nitrate tetrahydrate (99%) and magnesium nitrate tetrahydrate (99.8%), continue stirring with a magnetic stirrer for 90 min...
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Abstract
The invention relates to a preparation method and application of a tissue engineering biological scaffold, and belongs to the technical field of biological medicines. According to the invention, bioactive glass nanospheres and an acellular cartilage extracellular matrix are included; and the bioactive glass nanospheres and the acellular cartilage extracellular matrix are constructed into the composite scaffold. The composite scaffold not only has the advantages of a decellularized cartilage extracellular matrix scaffold, but also overcomes some defects of a pure decellularized cartilage scaffold, the biological activity of the scaffold is increased, meanwhile, the mechanical property of the scaffold is also increased, and the scaffold can better promote regeneration and repair of cartilage and osteocartilage. By utilizing the characteristics that the acellular cartilage extracellular matrix can be subjected to freeze-drying forming and chemical crosslinking, the defect that bioactive glass nanospheres are not easy to form as granular products is overcome, and the advantages of high specific surface area, solubility, ion slow release characteristic and the like of the bioactive glass nanospheres are fully utilized. The invention provides a good biological scaffold for tissue engineering.
Description
technical field [0001] The invention relates to a preparation method and application of a tissue engineering biological scaffold and belongs to the technical field of biomedicine. Background technique [0002] Cartilage damage is often accompanied by changes in subchondral bone, which can further lead to the occurrence and development of osteoarthritis. However, due to the lack of blood supply of cartilage itself, it is difficult to repair itself after injury, which has always been a difficult clinical problem to solve. In recent years, with the development of regenerative medicine technology, tissue engineering cartilage repair technology has brought new hope to the repair of cartilage damage, especially cartilage repair strategies based on tissue engineering scaffolds are becoming more and more hot spots in cartilage repair research. In the previous research work, the extracellular matrix of acellular cartilage was used to construct a cartilage-oriented scaffold (Chinese ...
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