40 results about "Extracellular matrix scaffold" patented technology

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition may also include an extracellular matrix scaffold component of any formulation, and further include added cells, proteins, or other components to optimize the regenerative process and restore cardiac function. Methods for regenerating defective or absent myocardium apply a composition to a site of myocardium in need of regeneration using a delivery mode appropriate for the particular formulation.

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition can also have an extracellular matrix scaffold component of any formulation, then including also added cells, proteins, or other components to optimize the regenerative process and restore cardiac function. Methods for regenerating defective or absent myocardium apply a composition to a site of myocardium in need of regeneration using a delivery mode appropriate for the particular formulation.

The present invention relates to a method for preparing a cell-derived ECM scaffold to which chondrocytes or stem cells are attached, a method for cartilage regeneration by tissue engineering, which comprises using the cell-derived ECM scaffold, and a therapeutic composition for treating cartilage disorder, which contains the ECM scaffold as an effective component. More specifically, the present invention relates to a method for cartilage regeneration by tissue engineering, which comprises transplanting ECM scaffold, having chondrocytes or stem cells attached thereto, into cartilage defects, and a therapeutic composition for treating cartilage disorder, which contains the ECM scaffold, having chondrocytes or stem cells attached thereto, as an effective component. According to the present invention, when the inventive ECM scaffold having chondrocytes or stem cells attached thereto is transplanted into a cartilage defect, mature articular cartilage having the same appearance and characteristics as those of natural cartilage tissue, can be regenerated without side effects such as inflammatory responses.

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition can also have an extracellular matrix scaffold component of any formulation, then including also added cells, proteins, or other components to optimize the regenerative process and restore cardiac function. Methods for regenerating defective or absent myocardium apply a composition to a site of myocardium in need of regeneration using a delivery mode appropriate for the particular formulation.

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition can also have an extracellular matrix scaffold component of any formulation, then including also added cells, proteins, or other components to optimize the regenerative process and restore cardiac function. Methods for regenerating defective or absent myocardium apply a composition to a site of myocardium in need of regeneration using a delivery mode appropriate for the particular formulation.

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition can also have an extracellular matrix scaffold component of any formulation, then including also added cells, proteins, or other components to optimize the regenerative process and restore cardiac function. Methods for regenerating defective or absent myocardium apply a composition to a site of myocardium in need of regeneration using a delivery mode appropriate for the particular formulation.

The invention relates to the field of tissue engineering materials, and in particular relates to a decellularization method for preparing an extracellular matrix (ECM) support material. By using green surfactant glucopyranoside for decellularization treatment, cells can be completely removed, and an ultra microstructure and compositions of an ECM support are maintained.

The subject invention pertains to materials and methods for processing tissue to produce a natural, acellular replacement tissue that is immunocompatible with a recipient. According to the subject invention, harvested tissue is subjected to wash solutions wherein only amphoteric detergent(s) are used (e.g., anionic detergents are excluded). Following extraction by amphoteric detergent(s), the tissue is washed with a buffer system to facilitate the clearance of cellular components and detergent. In one embodiment, the subject invention pertains to a replacement tissue that is a nerve graft that supports axonal regeneration, guides axons toward the distal nerve end and/or is immunologically tolerated. Preferably, the nerve graft retains essential extracellular matrix scaffolding as well as biological components that promote nerve regeneration through the nerve graft.

The invention belongs to the field of biological materials and discloses an extracellular matrix support material and a preparation method thereof. The extracellular matrix support material disclosed by the invention is prepared into a three-dimensional porous support material with a certain pore diameter and porosity through performing in-vitro monolayer high-density culture on immature rabbit bone marrow mesenchymal stem cells and collecting extracellular matrixes secreted by the immature rabbit bone marrow mesenchymal stem cells by applying the freeze-drying technology and a proper cross-linking agent. The material can be trimmed into proper sizes and shapes according to a cartilage defect zone or the requirement of an in-vitro experiment, and the material is sterilized by ethylene oxide to obtain an integral packaging bag. As the stem cells are used as material sources of an extracellular matrix support, the extracellular matrix support material disclosed by the invention is wide in tissue source and strong in multiplication capacity, therefore, the extracellular matrix support material can obtain enough cell quantity per se, and the problems of social ethics involved by embryonic stem cells are avoided.

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition may also include an extracellular matrix scaffold component of any formulation, and further include added cells, proteins, or other components to optimize the regenerative process and restore cardiac function.

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition can also include an extracellular matrix scaffold component of any formulation, and further include added cells, proteins, or other components to optimize the regenerative process and restore cardiac function.

The invention discloses a nucleus pulposus-cartilage extracellular matrix scaffold and a preparing method thereof and belongs to the biological tissue engineering technology. The preparing method comprises the steps that nucleus pulposuses and bony articular surface cartilage are smashed, decellularized, subjected to gradient centrifugation to collect extracellular matrix microfilament suspension, and freeze-dried to obtain a freeze-dried decellularized nucleus pulposus matrix and a freeze-dried decellularized cartilage matrix, the two matrixes are taken in certain proportion and dissolved in ultrapure water to prepare mixed matrix suspension with certain concentration, and the mixed matrix suspension is freeze-dried and subjected to ultraviolet irradiation crosslinking and chemical reagent crosslinking in sequence so that the nucleus pulposus-cartilage extracellular matrix scaffold can be obtained. In this way, the defect that the nucleus pulposuses are hard to obtain due to the small number is overcome; the preparing technology is simple, and decellularizing can be achieved thoroughly; the matrix scaffold is degradable and high in biocompatibility; the matrix scaffold is of a three-dimensional structure, is high in mechanical property and plasticity, and can facilitate the distribution, growth and differentiation of seed cells; the freeze-dried extracellular matrixes are convenient to store and prepare and can be used for constructing a tissue engineered intervertebral disc to repair partial defects of the intervertebral disc, and broad clinical application prospects are realized.

The invention discloses a hydrogel, and a preparation method and a three-dimensional cell culture method thereof. The preparation method of the hydrogel comprises the following steps: S1, preparing pig acellular dermis and/or pig acellular bladder; S2, freeze-drying the porcine acellular dermis and/or the porcine acellular bladder; S3, grinding the freeze-dried porcine acellular dermis and/or freeze-drying the porcine acellular bladder into fine particles; S4, adding the fine particles into pepsin digestive juice, and stirring for 48-72 hours to form an extracellular matrix scaffold; and S5, adjusting the pH value and the salt concentration of the pepsin digestive juice to inactivate the pepsin, and recombining the protein in the extracellular matrix scaffold to form the hydrogel.

The invention relates to an adipose extracellular matrix scaffold and a production method and application thereof. The production method of the adipose extracellular matrix scaffold comprises the following steps: subjecting adipose tissue to decellularization treatment, dehydration treatment and degreasing treatment in sequence to obtain an adipose extracellular matrix; crushing the adipose extracellular matrix to obtain matrix powder; subjecting the matrix powder and methacrylic anhydride to a reaction in a first buffer solution to obtain a methacrylated adipose extracellular matrix; and mixing the methacrylated adipose extracellular matrix, a photoinitiator and a second buffer solution, and then carrying out a cross-linking reaction under ultraviolet irradiation to obtain the adipose extracellular matrix scaffold. According to the production method of the adipose extracellular matrix scaffold, the degradation velocity of the adipose extracellular matrix scaffold can be adjusted according to the requirements, and the obtained adipose extracellular matrix has good mechanical strength.

The invention discloses a marine-derived bionic cartilage material and a preparation method thereof, which belong to the field of a medical material. The marine-derived bionic cartilage material is obtained by decellularizing a marine-derived cartilage material by a special process, and has a natural bionic cartilage structure, which can be used for cartilage repair to induce cartilage regeneration; and the preparation method of the marine-derived bionic cartilage material comprises the steps of pretreatment of raw materials, rough finishing, decellularization, re-finishing, preservation liquid pretreatment, molding and the like. The marine-derived bionic cartilage material prepared by the method of the invention has the natural network structure and good mechanical strength, and is a lowimmunogenic bionic extracellular matrix scaffold material, which can solve the problem of clinical cartilage repair; moreover, the marine-derived bionic cartilage material has low risk of carrying andspreading the zoonotic virus, rich resources and low cost, and can fill the market blank of cartilage repair products and has profound social significance.

The invention relates to a bionic artificial bone. The artificial bone comprises a component A and a component B, wherein the component A is a marine-source biomineralized substance with a natural multiple nano structure, and the component B is an artificial extracellular matrix organic substance. Specifically, the component B in a solution form is a complex of collagen and polysaccharide, and thecomponent A mainly consists of a marine organism exoskeleton of bacillariophyta. The invention also relates to a preparation method of the bionic artificial bone. The bionic artificial bone is bionically compounded of marine-source natural inorganic materials and organic materials, has the natural multiple nano bionic structure, can guide and induce the generation of new tissue, and comprises theinorganic mineralized components of the marine-source natural multiple nano structure and the organic components of an artificial extracellular matrix; the sources are wide, the cost is low, the biological safety is high, and therefore the bionic artificial bone is adopted as a natural bionic extracellular matrix stent material. The prepared bionic artificial bone has high processability and clinical operability and can meet the clinical demands of repairing different bone defects.

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition may also include an extracellular matrix scaffold component of any formulation, and further include added cells, proteins, or other components to optimize the regenerative process and restore cardiac function.

The invention discloses an ovarian extracellular matrix scaffold and hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biomedicine and clinical medicine. The preparation method of the ovarian extracellular matrix scaffold comprises the following steps: 1) ovarian tissue pretreatment; 2) multi-decellularization treatment: sequentially putting the ovarian tissue skin and marrow treated in the step 1) into double distilled water, a mixed solution of sodium deoxycholate and triton X-100 and a lauryl sodium sulfate solution, sufficiently shaking and washing, adding DNA enzyme, and culturing in a culture medium at 37 DEG C for 4-8 hours; (3) sterilization and freeze-drying treatment: (4) preparation of the ovarian extracellular matrix scaffold: grinding the freeze-drying tissue prepared in the step (3) into particles with the particle size of less than 1mm, and adding acid liquor, pepsase, alkali liquor and a buffer solution into the particles to prepare the ovarian extracellular matrix scaffold. and continuously adding granular cells and follicles into the ovarian extracellular matrix scaffold to prepare the hydrogel material which can be implanted into a mammal body and has the effects of repairing ovarian injury and reconstructing ovarian functions. The research provides a new strategy for female fertility protection.