Preparation method of gene-activated poly-lactic acid material for treating liver diseases

A technology of polylactic acid material and gene activation, which is applied in the field of gene activated polylactic acid material and its preparation, can solve the problems of integration and long-term stability between polylactic acid implants and surrounding tissues of the liver, and achieve good cell compatibility , Improve adhesion performance, promote repair and regeneration

Inactive Publication Date: 2014-03-05
CHONGQING UNIV
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Problems solved by technology

[0005] However, these methods still cannot solve the problems of integration of PLA implants with surrounding tissues of the liver, long-term stability, etc.

Method used

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  • Preparation method of gene-activated poly-lactic acid material for treating liver diseases

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Experimental program
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Embodiment

[0031] ①Preparation of galactosylated chitosan

[0032] Configure NaHCO 3 / acetic acid buffer solution (pH4.7 of the buffer solution, NaHCO in the solution 3 The concentration is 0.1N); Take lacturonic acid (LA) and dissolve it in NaHCO 3 / acetic acid buffer solution, prepared into a molar concentration of 64mM lacturonic acid solution, then added 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinyl Imine (NHS), in the resulting solution, the molar ratio of LA, NHS and EDC is 2:1:1, of course, the molar ratio of LA, NHS and EDC is 1:1:1-2: 1:1 is acceptable;

[0033]Stir magnetically at room temperature for 5 minutes to completely dissolve the material, then place it at 4°C for 3 hours, then react at room temperature for 3 hours, then add chitosan to the solution (the molar ratio of LA to chitosan is 2:1), and keep stirring For 3 days, dialyze in deionized water with a dialysis bag for 3 days, and obtain a galactose grafting rate of 10.9% galactosylat...

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Abstract

The invention discloses a gene-activated poly-lactic acid material for treating a liver disease, comprising a poly-lactic acid material body and an activating layer, wherein the activating layer comprises a plasmid DNA layer and a galactose glycosidation chitosan layer, the plasmid DNA layer and the galactose glycosidation chitosan layer are sequentially arranged from the interior to the exterior at intervals, the gene-activated poly-lactic acid material has excellent cellular compatibility, can induce the growth of liver cells in situ, can strengthen the adhesive performance of the liver cells on an extracellular matrix scaffold material, can improve the stability of an implant, further induces the transfer of liver targeted genes, efficiently regulates and controls the genes to release in a targeted manner, strengthens the activity of cells and increases the transfection efficiency so as to realize the treatment of the liver disease. The invention further provides a preparation method of the gene-activated poly-lactic acid material, wherein the galactose glycosidation chitosan layer and the plasmid DNA layer are sequentially deposited on the surface of the poly-lactic acid material by utilizing the electrostatic interaction between the galactose glycosidation chitosan molecules and the plasmid DNAs, the method has simple operation and low cost and is easy to implement and very suitable for the industrial application.

Description

technical field [0001] The invention relates to a gene-activated polylactic acid material for liver disease treatment and a preparation method thereof. Background technique [0002] The liver is the center of material metabolism in the human body and has functions such as metabolism and detoxification. Deaths due to liver diseases such as liver cancer, fatty liver, cirrhosis, and hepatitis have occurred frequently, seriously threatening human health. The development of ideal scaffold materials for liver tissue engineering is an important direction in the field of medicine and biomaterial science. [0003] Biomaterials refer to a class of materials that can repair, replace and regenerate body tissues and have special functions. The surface of biomaterials plays a vital role in biology and medicine, because after biomaterials are implanted into organisms, the first contact with the body to exert biological reactions is the surface / interface of biomaterials. Most of the biolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/34A61L27/18A61L27/54A61K48/00A61P1/16
Inventor 蔡开勇胡燕
Owner CHONGQING UNIV
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