Adipose extracellular matrix scaffold and production method and application thereof

Active Publication Date: 2020-07-07
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mechanical strength of fat extracellular matrix is ​​poor, and the degradation rate in vivo is t...

Method used

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  • Adipose extracellular matrix scaffold and production method and application thereof
  • Adipose extracellular matrix scaffold and production method and application thereof
  • Adipose extracellular matrix scaffold and production method and application thereof

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preparation example Construction

[0035] Such asfigure 1 As shown, the preparation method of the adipocyte extracellular matrix scaffold in one embodiment comprises the following steps:

[0036] Step S110: Perform pretreatment on the adipose tissue.

[0037] Wherein, the step of pretreating the adipose tissue includes: washing the adipose tissue with physiological saline to remove the blood in the adipose tissue; then mixing the washed adipose tissue with water for homogenization and collecting the precipitate.

[0038] Specifically, the adipose tissue may be animal fat or isolated human fat, wherein the isolated human fat may be extracted human fat, for example.

[0039] Specifically, the step of washing the adipose tissue with physiological saline includes: chopping the adipose tissue, mixing it with physiological saline, and centrifuging at 1000 rpm to 2000 rpm for 3 minutes to 10 minutes to remove blood in fat.

[0040] Specifically, the step of mixing the washed adipose tissue with water for homogenizat...

Embodiment 1

[0076] The preparation process of the adipocyte extracellular matrix scaffold of the present embodiment is as follows:

[0077] (1) Pretreatment of adipose tissue: cut fresh porcine adipose tissue into small pieces with a size of about 1cm×1cm×1cm, then mix with normal saline, and centrifuge at 1200 rpm for 8 minutes to remove fat in the blood. Mix the washed adipose tissue with pure water at a mass ratio of 0.5:1, then homogenize in a high-speed homogenizer at room temperature at a speed of 12,000 rpm for 6 minutes, and place the homogenized tissue suspension to obtain a suspension The suspension was centrifuged at 4° C. for 3 minutes at a speed of 3000 rpm, and the white precipitate was collected to obtain pretreated adipose tissue.

[0078] (2) Decellularization treatment: mix the pretreated adipose tissue obtained in step (1) with an aqueous solution of sodium hydroxide with a mass percentage concentration of 1.0% in a mass ratio of 1:20, and then at 25° C. Incubate in a...

Embodiment 2

[0084] The preparation process of the adipocyte extracellular matrix scaffold of the present embodiment is as follows:

[0085] (1) Pretreatment of adipose tissue: the body fat collected by liposuction was mixed with normal saline, and centrifuged at 2000 rpm for 10 minutes to remove the blood in the fat. Mix the washed adipose tissue with pure water at a mass ratio of 3:1, then homogenize in a high-speed homogenizer at room temperature at a speed of 10,000 rpm for 3 minutes, and let the homogenized tissue suspension stand still to obtain a suspension Liquid, the suspension was centrifuged at 10° C. for 10 minutes at a speed of 5000 rpm, and the white precipitate was collected to obtain pretreated adipose tissue.

[0086] (2) Decellularization treatment: mix the pretreated adipose tissue obtained in step (1) with an aqueous solution of sodium hydroxide with a mass percentage concentration of 2% in a mass ratio of 1:10, and then at 20° C. Incubate in a shaking shaker for 4 hou...

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Abstract

The invention relates to an adipose extracellular matrix scaffold and a production method and application thereof. The production method of the adipose extracellular matrix scaffold comprises the following steps: subjecting adipose tissue to decellularization treatment, dehydration treatment and degreasing treatment in sequence to obtain an adipose extracellular matrix; crushing the adipose extracellular matrix to obtain matrix powder; subjecting the matrix powder and methacrylic anhydride to a reaction in a first buffer solution to obtain a methacrylated adipose extracellular matrix; and mixing the methacrylated adipose extracellular matrix, a photoinitiator and a second buffer solution, and then carrying out a cross-linking reaction under ultraviolet irradiation to obtain the adipose extracellular matrix scaffold. According to the production method of the adipose extracellular matrix scaffold, the degradation velocity of the adipose extracellular matrix scaffold can be adjusted according to the requirements, and the obtained adipose extracellular matrix has good mechanical strength.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a fat cell extracellular matrix scaffold and its preparation method and application. Background technique [0002] In recent years, soft tissue injuries caused by congenital malformations, chronic diseases, infections, tumor resections, or traumas have been increasing every year, leading to an increase in medical costs and patient morbidity. However, for soft tissue injuries, the ideal surgical treatment is to completely resect the defective tissue first, and then replace the defective tissue and reconstruct the structure and function of the defective tissue by whole organ transplantation or autologous transplantation. However, the lack of availability to transplant soft tissue, the relative complexity of tissue matching, and the high risk of morbidity at the donor site make whole organ transplantation or autologous transplantation for soft tissue defects relatively difficult ...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/36
CPCA61L27/50A61L27/3633A61L27/3687A61L27/3691A61L2430/34A61L2430/40
Inventor 朱勇军李丽花钟梅玲康文亭佘振定谭荣伟
Owner SHENZHEN LANDO BIOMATERIALS
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