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Marine-derived bionic cartilage material and preparation method thereof

A bionic and cartilage technology, applied in the medical field, can solve problems such as poor functionality, high biological risk, and high risk, and achieve the effects of low risk of carrying and spreading, low risk of virus transmission, and wide-ranging sources

Inactive Publication Date: 2019-09-03
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Cartilage injury repair is a worldwide problem, and there is no dedicated clinical product at present; currently, collagen materials (type I collagen, derived from terrestrial animals) or collagen composite materials are mostly used as cartilage repair materials, but the effect is not good: ①Poor functionality: for Fibrocartilage repair, non-hyaline cartilage repair; ②Poor compatibility: cartilage collagen is type II, while commercially available collagen products are all type I, and the risk of chronic inflammatory reaction after implantation is high; ③Mechanical strength is not good Good; ④ High biological risk of virus transmission
There are currently no commercially available type II collagen medical material products

Method used

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  • Marine-derived bionic cartilage material and preparation method thereof
  • Marine-derived bionic cartilage material and preparation method thereof
  • Marine-derived bionic cartilage material and preparation method thereof

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preparation example Construction

[0036] refer to figure 1 As shown, the present invention provides a method for preparing marine-derived biomimetic cartilage material, comprising the steps of:

[0037] (1) Raw material pretreatment: Remove redundant tissues and impurities from the marine-derived cartilage material, cut it into a suitable size, and freeze it at a low temperature for later use;

[0038] (2) Pretreatment: at 4°C, stir the cartilage material treated in step (1) with a 2%-5% NaCl solution with a solid-liquid ratio of 1:5-1:20 (w / w) for 1-4 hours and then remove For waste liquid, repeat 2-4 times; then perform rough finishing to remove residual redundancy;

[0039] (3) Microstructure protection: at 4°C, stir the pretreated cartilage material in step (2) with a phosphate buffer solution with a solid-to-liquid ratio of 1:5-1:20 (w / w) for 2-4 hours, and then remove the waste solution; the above steps were repeated 1-3 times;

[0040] (4) Cleaning: at 4°C, stir the cartilage material treated in step...

Embodiment 1

[0056] (1) Raw material pretreatment: remove redundant and other tissues from the fresh anglerfish cartilage, rinse it with deionized water at 4°C for several times; then cut it into appropriate sizes according to different anatomical parts, with a thickness of about 0.3cm; then quickly place it at -40 ℃ frozen, take out and thaw under running water at room temperature before use.

[0057] (2) Pretreatment: at 4°C, stir the cartilage material treated in step (1) with a 5% NaCl solution with a solid-to-liquid ratio of 1:20 (w / w) for 3 hours, then remove the waste liquid, and repeat twice; Roughly organize and remove some residual redundancy.

[0058] (3) Microstructure protection: Stir the pretreated cartilage material in step (2) with phosphate buffer (0.2M, pH7.2) at a solid-to-liquid ratio of 1:20 (w / w) for 2 hours at 4°C Remove the waste liquid; repeat the above steps 2 times.

[0059] (4) Cleaning: at 4°C, the cartilage material treated in step (3) was stirred with deion...

Embodiment 2

[0067] (1) Raw material pretreatment: remove redundant and other tissues from the fresh anglerfish cartilage, rinse it with deionized water at 4°C for several times; then cut it into appropriate sizes according to different anatomical parts, with a thickness of about 0.3cm; then quickly place it at -40 ℃ frozen, take out and thaw under running water at room temperature before use.

[0068] (2) Pretreatment: at 4°C, stir the cartilage material treated in step (1) with a 6% NaCl solution with a solid-to-liquid ratio of 1:20 (w / w) for 3 hours, then remove the waste liquid, and repeat twice; Roughly organize and remove some residual redundancy.

[0069] (3) Microstructure protection: Stir the pretreated cartilage material in step (2) with phosphate buffer (0.2M, pH7.2) at a solid-to-liquid ratio of 1:20 (w / w) for 2 hours at 4°C Remove the waste liquid; repeat the above steps 2 times.

[0070] (4) Cleaning: at 4°C, the cartilage material treated in step (3) was stirred with deion...

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Abstract

The invention discloses a marine-derived bionic cartilage material and a preparation method thereof, which belong to the field of a medical material. The marine-derived bionic cartilage material is obtained by decellularizing a marine-derived cartilage material by a special process, and has a natural bionic cartilage structure, which can be used for cartilage repair to induce cartilage regeneration; and the preparation method of the marine-derived bionic cartilage material comprises the steps of pretreatment of raw materials, rough finishing, decellularization, re-finishing, preservation liquid pretreatment, molding and the like. The marine-derived bionic cartilage material prepared by the method of the invention has the natural network structure and good mechanical strength, and is a lowimmunogenic bionic extracellular matrix scaffold material, which can solve the problem of clinical cartilage repair; moreover, the marine-derived bionic cartilage material has low risk of carrying andspreading the zoonotic virus, rich resources and low cost, and can fill the market blank of cartilage repair products and has profound social significance.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a marine-derived bionic cartilage material and a preparation method thereof. Background technique [0002] Cartilage injury repair is a worldwide problem, and there is no dedicated clinical product at present; currently, collagen materials (type I collagen, derived from terrestrial animals) or collagen composite materials are mostly used as cartilage repair materials, but the effect is not good: ①Poor functionality: for Fibrocartilage repair, non-hyaline cartilage repair; ②Poor compatibility: cartilage collagen is type II, while commercially available collagen products are all type I, and the risk of chronic inflammatory reaction after implantation is high; ③Mechanical strength is not good Good; ④ The biological risk of virus transmission is high. Currently there is no commercially available type II collagen medical material product. Contents of the invention [0003] In ord...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3612A61L27/3654A61L27/3687A61L27/50A61L2430/06
Inventor 位晓娟张长青林途唐千朱振中
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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