Preparation method of a specific demineralized extracellular matrix scaffold of cross-linked antibiotics

A technology of antibiotics and extracellular matrix, applied in the field of bone infection treatment, can solve the problems of inability to prevent re-infection of "latent" bacteria, unfavorable for tissue repair and osteogenesis, and no longer have antibacterial properties, so as to achieve sufficient reference, prevention and treatment. Bone infection, significant anti-infective effect

Active Publication Date: 2022-08-05
杭州源囊生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

First of all, due to the difference in surface free energy between inorganic and organic media, this modification is not simple, and the unstable coating will inevitably fall off with the passage of time and changes in the internal environment, resulting in a decrease in the biocompatibility of the material; Secondly, with the complete release of antibiotics or antimicrobial peptides, the material itself no longer has antibacterial properties and becomes the object of bacterial adhesion, and cannot prevent the reinfection of "latent" bacteria; finally, metal ions are cytotoxic, which is not conducive to tissue repair and osteogenesis

Method used

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  • Preparation method of a specific demineralized extracellular matrix scaffold of cross-linked antibiotics
  • Preparation method of a specific demineralized extracellular matrix scaffold of cross-linked antibiotics
  • Preparation method of a specific demineralized extracellular matrix scaffold of cross-linked antibiotics

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Embodiment 1

[0076] The preparation method of cross-linked vancomycin-specific demineralized extracellular matrix scaffold (Van-SDECM), the specific steps are as follows:

[0077] (1) The cancellous bone is obtained from the fresh pig shoulder blade with a hollow bone drill with a diameter of 6mm, and then cut into a cylinder with a thickness of 3mm;

[0078] (2) Rinse the cancellous bone block with tap water for 1h, package it in an embedding box, and then soak it in 0.6% (v / v) peracetic acid ultrapure water 1; 1% (v / v) Triton-X100 After filtering and sterilizing with a 0.22 μm filter head, transfer the embedding cassette to a flask of Triton-X100 solution, shake at 100 rpm and 4°C for 24 h; wash the embedding cassette with 500 ml sterilized water, and continue stirring for 1 h, repeating twice; Subsequently, the cancellous bone blocks were added to 1% (w / v) sodium dodecyl sulfate (SDS) aqueous solution, shaken at 100 rpm and 4°C for 36 h, and the embedding cassette was washed again;

[...

Embodiment 2

[0082]The preparation method of cross-linked vancomycin-specific demineralized extracellular matrix scaffold (Van-SDECM), the specific steps are as follows:

[0083] (1) The cancellous bone is obtained from the fresh pig shoulder blade with a hollow bone drill with a diameter of 4mm, and then cut into a cylinder with a thickness of 2mm;

[0084] (2) Rinse the cancellous bone block with tap water for 1h, package it in an embedding box, and then soak it in 0.6% (v / v) peracetic acid ultrapure water 1; 1% (v / v) Triton-X100 After filtering and sterilizing with a 0.22 μm filter head, transfer the embedding cassette to a flask of Triton-X100 solution, shake at 100 rpm and 4°C for 12 h; wash the embedding cassette with 500 ml of sterilized water, and continue stirring for 1 h, repeating twice; Subsequently, the cancellous bone block was added to a 1% (w / v) sodium dodecyl sulfate (SDS) aqueous solution, shaken at 100 rpm and 4°C for 12 h, and the embedding cassette was washed again;

...

Embodiment 3

[0088] The preparation method of cross-linked vancomycin-specific demineralized extracellular matrix scaffold (Van-SDECM), the specific steps are as follows:

[0089] (1) The cancellous bone is obtained from the fresh porcine shoulder blade with a hollow bone drill with a diameter of 8mm, and then cut into a cylinder with a thickness of 4mm;

[0090] (2) Rinse the cancellous bone block with tap water for 1h, package it in an embedding box, and then soak it in 0.6% (v / v) peracetic acid ultrapure water 1; 1% (v / v) Triton-X100 After filtering and sterilizing with a 0.22 μm filter head, transfer the embedding cassette to a flask of Triton-X100 solution, shake at 100 rpm and 4°C for 48 h; wash the embedding cassette with 500 ml of sterilized water, and continue stirring for 1 h, repeat twice; Subsequently, the cancellous bone blocks were added to a 1% (w / v) sodium dodecyl sulfate (SDS) aqueous solution, shaken at 100 rpm, 4°C for 48 h, and the embedding cassette was washed again; ...

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Abstract

The invention discloses a preparation method of a cross-linked antibiotic specific demineralized extracellular matrix scaffold, and belongs to the technical field of bone infection treatment. The invention discloses a method for preparing a specific demineralized extracellular matrix scaffold of cross-linked antibiotics. The specific demineralized and decellularized cancellous bone extracellular matrix scaffold (SDECM) is used as a substrate, and the specific demineralized and decellularized cancellous bone extracellular matrix scaffold (SDECM) is used as a substrate, and the antibiotic is electrostatically adsorbed and chemically adsorbed. Cross-linking two forms of composite drug loading, releasing antibiotics through rapid pH response and accompanying material degradation in an acidic environment, anti-infection through antibiotics and promoting repair through SDECM, so as to achieve pH-sensitive drug release in an acidic environment in the early stage of infection Rapid sterilization, sustained sterilization by slow release of drugs in the mid-late physiological environment, and permanent sterilization ability to prevent infection recurrence in the absence of infection provide new ideas and tools for the antibacterial and repairing treatment of bone infections.

Description

technical field [0001] The invention relates to the technical field of bone infection treatment, and more particularly to a preparation method of a cross-linked antibiotic-specific demineralized extracellular matrix scaffold. Background technique [0002] Despite the continuous improvement of medical level, bone infection is still a huge problem in clinical work, and nearly 40% of patients are faced with the recurrence and prolonged infection of infection. In the pathological process of bone infection, bacteria multiply and form abscesses in the local area of ​​infection, evading the immune system to hunt down and protect the reproduction of viable bacteria at the core, the factors secreted by bacteria and acidic metabolites in the abscess not only promote the differentiation of osteoclasts , reduce the activity of osteoblasts, and at the same time help bacteria to adhere to collagen or cell membranes, promote the formation of biofilms, and lead to persistent bone infection....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/00A61L27/50A61L27/54
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/3608A61L27/365A61L27/00A61L27/50A61L27/54A61L2430/40A61L2430/02A61L2300/602A61L2300/412A61L2300/406A61L2300/41
Inventor 林贤丰顾辰辉方斌范顺武
Owner 杭州源囊生物科技有限公司
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