Preparation method and application of extracellular matrix scaffold with controllable pore structure

A technology of acellular matrix and pore structure, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of long material preparation cycle and uncontrollable material composition, and achieve the effect of shortening the preparation cycle

Inactive Publication Date: 2022-03-18
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method and application of an extracellular matrix scaffold with controllable pore structure, so as to solve the problems of long material preparation cycle and uncontrollable material composition in the prior art

Method used

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  • Preparation method and application of extracellular matrix scaffold with controllable pore structure
  • Preparation method and application of extracellular matrix scaffold with controllable pore structure
  • Preparation method and application of extracellular matrix scaffold with controllable pore structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Porcine Liver Acellular Matrix Scaffold with Controllable Pore Structure Using PCL Microsphere Aggregates as Template

[0037]Preparation of polycaprolactone (PCL) microsphere template: PCL microspheres were prepared by emulsification solvent evaporation method. Weigh 10.0 g of PCL with a molecular weight of 80,000, add it into 200 mL of dichloromethane, stir and dissolve at room temperature until clear, and prepare a PCL solution with a concentration of 50 g / L. The obtained PCL solution was added to 1800mL of 2% polyvinyl alcohol (PVA) solution by mass fraction, stirred at 1000rpm, volatilized at room temperature to remove dichloromethane, left to settle to collect the microspheres at the bottom, and washed 5 times with distilled water Residual PVA was removed, and the collected microsphere particles were freeze-dried to obtain PCL microspheres, which were then filtered through different meshes to obtain PCL microspheres with different diameters (diamete...

Embodiment 2

[0043] Preparation of porcine intestinal mucosa acellular matrix scaffold with controllable pore structure using PLA microsphere aggregates as template

[0044] Preparation of PLA microspheres: Weigh 8.0 g of PLA with a molecular weight of 200,000, add it into 200 mL of chloroform, stir and dissolve at room temperature until clear, and prepare a PLA solution with a concentration of 40 g / L. Add the above PLA solution to 1800mL of 3% polyvinyl alcohol (PVC) solution with a mass fraction of 3%, stir at 800rpm, volatilize and remove chloroform at room temperature, collect the microspheres by static precipitation, wash with distilled water 5 times to remove residual PVA , the collected microspheres were freeze-dried to obtain PLA microspheres. Then, it was filtered with different meshes to obtain PLA microspheres with different diameters (diameter range: 40-500 μm). Place 0.4 g of PLA microspheres with different diameters in circular holes with a diameter of 1.0 cm and a height of...

Embodiment 3

[0050] Preparation of bovine Achilles tendon tissue acellular matrix scaffold with controllable pore structure using PLGA microsphere aggregates as template

[0051] Preparation of PLGA microspheres: Weigh 10.0 g of PLGA with a molecular weight of 20,000, add it into 100 mL of chloroform, stir and dissolve at room temperature until clear, and prepare a PLGA solution with a concentration of 100 g / L. The above PLGA solution was added to 1900mL of 1% PVA solution with a mass fraction of 1900mL, stirred at 1200rpm, volatilized at room temperature to remove chloroform, left to settle to collect microspheres, washed with distilled water 5 times to remove residual PVA, and collected microspheres The pellets were freeze-dried. Then, the PLGA microspheres with different diameters (diameter range: 40-500 μm) were obtained by filtering with different meshes. 1.0 g of PLGA microspheres with different diameters were placed in circular holes with a diameter of 2.0 cm and a height of 0.8 cm...

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Abstract

The invention discloses a preparation method and application of an extracellular matrix scaffold with a controllable pore structure, firstly, a polymer microsphere aggregate template is prepared, secondly, tissue is subjected to decellularization treatment to obtain an extracellular matrix material, and the extracellular matrix material is dissolved to obtain an extracellular matrix solution; thirdly, pouring the obtained extracellular matrix solution into pores of the microsphere aggregate to obtain a polymer-acellular matrix composite material; fourthly, cross-linking and neutralizing after freeze drying; and 5, eluting the polymer template to obtain the extracellular matrix scaffold material with the controllable pore structure. The method is short in material preparation period, controllable in matrix component and controllable in mechanics.

Description

technical field [0001] The invention relates to the technical field of tissue engineering scaffold material preparation, in particular to a preparation method and application of an extracellular matrix scaffold with controllable pore structure. Background technique [0002] Scaffold materials can provide cells with a biomimetic microenvironment, regulate cell migration, proliferation and differentiation and other behaviors, and can be used to load cells or guide tissue regeneration in situ. An ideal tissue engineering scaffold material should meet the following conditions: good biocompatibility, controllable pore structure, high porosity and connectivity, degradability, ability to load biomacromolecules and maintain their activity, etc. In general, scaffolds must have an appropriate pore structure to facilitate the transport of nutrients and the formation of capillaries, as well as the growth and infiltration of cells. The spherical porous and highly connected scaffold has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/56
CPCA61L27/3633A61L27/3687A61L27/3604A61L27/3629A61L27/50A61L27/56A61L2300/412A61L2430/40
Inventor 朱美峰孔德领李雯
Owner NANKAI UNIV
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