15results about How to "The experiment process is simple" patented technology

The invention belongs to the technical field of cell biology, and particularly relates to a method for detecting cell stability, a cell sample is detected in three aspects of cell performance, cell mutation and cell stability, and when the cell proliferation effect is good, the cell does not migrate, and the cell activity is gt; the cell of which the cell concentration is 80%, the cell early-stage and late-stage apoptosis ratio is less than or equal to 10%, the cell autophagy ratio is less than 5%, no proto-oncogene is remarkably expressed, the cell aging ratio is less than or equal to 20% and the cell multiplication time is less than 48 hours is a stable cell; if one of the indexes does not reach the standard, it is determined that cell passage is unstable, and the cell culture process is stopped. The method provided by the invention can comprehensively judge the life cycle of the cell, so that the cell is applied to the optimal economic / efficient service life, the cell state can be effectively and accurately judged, and the method has good reliability.

The invention discloses a quaternary ammonium salt modified magnetic microsphere and a preparation method thereof, and relates to the technical field of polymer microsphere preparation, and the preparation method comprises the following steps: modifying an epoxy group of a silicon hydroxyl magnetic microsphere through an epoxy group silane coupling agent, extending a spacer arm through a diamine compound, and coating the surface of the microsphere with a quaternary ammonium salt group. In the invention, the surface of the magnetic microsphere is coated with a large number of quaternary ammonium salt groups, the ligands have good physical and chemical stability, and quaternary ammonium salt spacer arms on the surface of the microsphere are adjusted by selecting diamine compounds with different carbon chain lengths, so that quaternary ammonium salt magnetic microspheres with different spacer arms can be obtained.

The invention discloses a performance evaluation method for a refrigerated infrared imaging system based on gas leakage, relates to the technical field of refrigerated infrared imaging systems, and solves the technical problem that the applicability of the prior art in terms of leaked gas detection is limited. Specifically, the background black body temperature, gas temperature, ambient pressure, ambient temperature, test gas type, and test optical system parameters in the experiment are controlled to remain consistent; the distances L1, L2, L3, ..., L n At equal distances, the minimum detectable flow rate of the system is measured to be LR1, LR2, ...LR n ; According to LR i =αL i 2 +β basic form for LR1...LR n With L1...L n The relationship between is fitted to obtain the specific comparison parameter α and the correction term β; the detection capability of the refrigerated infrared imaging system for gas leakage is determined by α, and the smaller α is, the stronger the detection capability of the refrigerated infrared imaging system for gas leakage is.

The invention relates to the field of gel, and discloses a PEI-CQDs composite material, gel and preparation methods of the PEI-CQDs composite material and the gel, and the preparation method of the PEI-CQDs composite material comprises the following steps: (1) electrolyzing graphite in water to obtain carbon quantum dots; and (2) carrying out a reaction on the carbon quantum dots and polyethyleneimine in the presence of an optional auxiliary agent to obtain the PEI-CQDs composite material. The PEI-CQDs composite material can be applied to the preparation process of gel, so that PEI with low molecular weight can also be used for preparing gel, and the gelation time can be shortened.

The invention relates to the technical field of gas pressure detection, in particular to a non-contact gas pressure measurement method based on dual-wavelength absorption. The method comprises the following steps: acquiring two characteristic absorption wavelengths lambda 1 and lambda 2 of gas to be measured and absorbance alpha 1 and alpha 2 of the gas to be measured at the temperature T and the wavelengths lambda 1 and lambda 2, calculating Lorentz full width at half maximum, performing Voigt linear fitting on the measured absorbance alpha 1 and alpha 2 respectively, and finally obtaining Lorentz full width at half maximum w1 and w2 of the absorbance alpha 1 and alpha 2 through Voigt linear fitting. And calculating the gas pressure P. According to the method, the dependence on the concentration in pressure measurement is eliminated by utilizing two absorption lines of gas molecules, and the measurement method is easily combined with an existing dual-wavelength absorption non-contact gas temperature measurement method to realize simultaneous measurement of the temperature, the pressure and the concentration of the gas.

The invention discloses an automobile cabin waterproof performance detection method, which comprises the following steps of S1, preparing a test vehicle, and preprocessing the test vehicle to enable the test vehicle to meet test requirements; s2, determining a waterproof test area of the to-be-tested vehicle; s3, connecting a detection device to a to-be-tested vehicle through an OBD interface, and then starting the vehicle; S4, splashing water in the waterproof test area, and then reading the fault code of the whole vehicle through the OBD interface in real time by the detection device to obtain a fault code signal of the vehicle after watering; and S5, the upper computer is connected with the detection equipment, and fault codes of the electric appliances in the vehicle intelligent cabin in the test process are obtained and used for evaluating the waterproof performance of the intelligent cabin. The method has the advantages that the waterproof performance of the vehicle can be rapidly detected, the waterproof performance of the vehicle can be quantitatively or qualitatively judged according to the obtained data in the experiment process, and research and development of an intelligent cabin of the vehicle can be guided accordingly.

The present invention discloses a method for determining the content of L-lysine hydrochloride and its application, which belongs to the chemical composition detection technology. The method comprises the following steps: sample group: mixing a solution of L-lysine hydrochloride to be tested with a quantitative and excess amount of silver nitrate solution, and Fe 3+ as an indicator, and titrate excess silver nitrate with a standard thiocyanate titration solution until a light reddish brown color appears and remains unfaded for 30 seconds as the titration endpoint; blank group: except for not adding the L-lysine hydrochloride to be tested, the rest of the operations are the same as the sample group; the content of L-lysine hydrochloride in the L-lysine hydrochloride to be tested is calculated according to the following formula: This method is simple and fast, has good repeatability, is economical and affordable, has a wide range of applications, and the analysis results are accurate and reliable. It can reduce the use of toxic reagents, reduce reagent management costs and laboratory safety risks, and can be used to detect the content of L-lysine hydrochloride in feed, improve feed quality and feeding effects.

This invention discloses the application of a silver group cluster composite catalyst in the epoxy ring-opening reaction. The silver group cluster composite catalyst of this invention is Ag. 32 / AC, where Ag 32 =Ag 32 (dppp)4(CCR) 22 dppp = 1,3-bis(diphenylphosphine)propane, CCR = 4-tert-butylphenylacetylene, AC = supercapacitor activated carbon. The silver group cluster composite catalyst provided by this invention exhibits excellent catalytic activity in the ring-opening reaction of styrene and methanol. The catalytic system of this invention operates under mild reaction conditions, achieves 100% selectivity, and efficiently and directionally generates the target product 2-methoxy-2-phenylethanol, effectively suppressing side reactions and providing a feasible route for the green and efficient synthesis of this type of product.

The invention discloses a preparation method and application of banana peel-based potassium-rich biochar and hydrogen-rich gas, the preparation method comprises the following steps: mixing banana peel and water according to a mass ratio of 4: 1-1: 4, carrying out in-situ gasification on the obtained mixed raw material at a high temperature of 800-900 DEG C to prepare the potassium-rich biochar, and obtaining a large amount of hydrogen-rich gas in the reaction process. The preparation method is simple, the potassium content in the biochar is greatly increased, the thought of biochar organic fertilizer utilization and banana peel utilization is expected to be developed, and waste resource utilization and agricultural sustainable development are achieved.

The invention discloses a coal bed gas horizontal well drilling fluid plugging performance evaluation method, and relates to the technical field of oil and gas exploitation, coal dust and coal gangue returned by a coal bed gas horizontal well are taken, dried and crushed, a cementing agent is added, a glue-containing mixture is prepared, and curing and cutting are performed to obtain a coal rock plate or a coal rock plate with microcracks; a coal rock plate is used as a filtration medium of the plugging instrument, and the measured plugging filtration loss and the plugging filtration loss reduction rate are used as evaluation indexes for evaluating the coal rock plugging anti-collapse performance of the drilling fluid; a coal rock plate with micro-cracks is used as a filtration medium of the plugging instrument, and the measured plugging filtration loss and the plugging filtration loss reduction rate are used as evaluation indexes for evaluating the plugging anti-collapse performance of the drilling fluid on coal rock with micro-crack development, so that the anti-collapse performance of horizontal well water-based drilling fluid such as coal bed gas and the like can be reflected more reliably, quickly and accurately; the method is used for guiding safe and rapid construction of horizontal wells easy to collapse, such as deep coal bed gas and carbonaceous mudstone.

The application discloses a RAPD molecular marker primer for identifying the gender of Momordica grosvenori. The nucleotide sequence of the RAPD molecular marker primer is ACAGCCTGCT. The application discloses a kit containing the RAPD molecular marker primer for identifying the gender of Momordica grosvenori and application thereof. The application also discloses a method for identifying the gender of Momordica grosvenori by using the RAPD molecular marker primer. The method comprises the following steps: performing PCR amplification on total DNA of Momordica grosvenori by using the RAPD molecular marker primer, performing electrophoresis to obtain corresponding bands, and then identifying the gender of Momordica grosvenori. The RAPD molecular marker primer provided by the application is a random primer, has high specificity for male plants, can efficiently and rapidly distinguish male plants from female plants of Momordica grosvenori, and has the advantages of small DNA dosage, low purity requirement, simple experimental process, fast working progress and low cost in the identification test.

The invention belongs to the field of structural strength testing, and particularly relates to a honeycomb equivalent parameter simulation method based on probability distribution. At present, the research on honeycomb equivalent parameter simulation does not fully consider the probability distribution problem caused by dispersity. The method comprises the following steps: scanning random N honeycombs, and measuring structural parameters of each honeycomb; performing statistical analysis on each structural parameter measurement value of the honeycomb to obtain a corresponding probability density function; performing Monte Carlo simulation based on the obtained probability density function to generate a random number sequence; in combination with the random number sequence and a cellular equivalent parameter formula, simulating to obtain a cellular equivalent parameter simulation value; and carrying out probability statistical analysis, establishing a frequency distribution histogram of the honeycomb equivalent parameters, obtaining a probability density function of the honeycomb equivalent parameters, and obtaining corresponding honeycomb equivalent parameter values in combination with given reliability. Experiments are simple and cost is low.

The present application relates to the field of fluorescent powder preparation, especially to a red light fluorescent powder coated with quantum dots and a preparation method and application thereof.A red light fluorescent powder with high light-emitting thermal stability and high temperature resistance is excited by blue light, and the general formula of the combination ratio is K 1.9 (MEA) 0.1 TiF6: x Mn 4+ @GQDs y mg / mol ; wherein 0.01<= x <=0.12, 1<= y <=10.The preparation method is ion exchange method and hydrothermal synthesis method, the raw materials are mixed and stirred at room temperature, then KF*2H2O, HF, K 1.9 (MEA) 0.1 TiF6: x Mn 4+ and GQDs are mixed and fully reacted, and the obtained mixture is washed and dried to obtain the final product.The fluorotitanate red light fluorescent powder of the present application can be excited by the blue light emitted by InGaN chip, has excellent high temperature fluorescent performance and heat resistance, and has good market prospect.

The invention provides a primer group and a method for detecting miRNA (micro Ribonucleic Acid) through one-pot real-time fluorescent quantitative PCR (Polymerase Chain Reaction), the primer group comprises a specific stem-loop primer LP, a specific Linker primer, a universal upstream primer F, a universal downstream primer R and a specific probe P, and the amplification specificity can be ensured; all reagents required by reaction are added into a reaction system at one time, and the experimental process is simple and easy to operate; the reverse transcription process and the qPCR process are carried out in one reaction tube, so that the repeated uncovering process is avoided, the pollution risk is reduced, and the personal error is reduced; the whole reaction time is greatly shortened compared with that of two-step fluorescent quantitative PCR, and the experiment time is saved; due to the design of the universal upstream primer and the universal downstream primer, multiple amplification of miRNA becomes possible.

This invention provides a method based on β'' A method for preparing Al2O3 and N-doped C composite-coated Na3V2(PO4)2F3 electrode material. First, citric acid, vanadium pentoxide, ammonium dihydrogen phosphate, and sodium fluoride are sequentially dissolved in deionized water and stirred at 60°C until gel formation occurs. After drying, the resulting powder is placed in a tube furnace and subjected to a two-step heat treatment process to obtain Na3V2(PO4)2F3. Then, Na3V2(PO4)2F3 and dopamine hydrochloride are dispersed in an aqueous solution of tris(hydroxymethyl)aminomethane hydrochloride, stirred, and then... β'' -Al2O3, after stirring for another 23 h, was filtered, dried, and then placed in a tube furnace for heat treatment to finally obtain β'' A Na3V2(PO4)2F3 electrode material was developed by coating Al2O3 and N-doped C. This electrode material is simple to prepare, and sodium-ion half-cells assembled using this material as the positive electrode exhibit ideal capacity performance and stable cycle performance, demonstrating potential application value in sodium-ion batteries.