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38results about How to "Screened" patented technology

Preparation method of carbon supported core-shell Ni-Pt particles for direct methanol fuel cells

The invention provides a method for preparing carbon-supported nucleocapsid type Ni-Pt particles for direct methanol fuel cell catalysts, which belongs to a preparation process of direct methanol fuel cell catalysts. The method comprises the steps of adopting sodium citrate as a stabilizer, adopting cationic surfactant CTAB as dispersant, using sodium hypophosphite to reduce nickel acetate, generating a Ni kernel on the surface of Vulcan XC-72 or mesoporous carbon treated with sodium borohydride, washing superfluous sodium hypophosphite and generating a Pt shell on the surface of the Ni kernel through chemical replacement. The catalyst has a structure with the Ni kernel and the Pt shell, and has the advantages of low Pt support amount and high catalytic activity.
Owner:NANJING UNIV OF AERONAUTICS & ASTRONAUTICS

Gene engineering monoclonal antibody combined with A-beta oligomer specificity

The invention relates to the technical field of genetic engineering antibody and provides a monoclonal antibody. The variable region of heavy chain of the monoclonal antibody contains the amino acid sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEO ID NO.3; the variable region of light chain of the monoclonal antibody contains the amino acid sequences shown in SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The invention also specifically provides a humanization single-chain antibody generated by the recombinant strain of enterotoxigenic Escherichia coli with the accession number CGMCC No.2821 and the amino acid sequence of the humanization single-chain antibody is shown in SEQ ID NO.7. The antibody of the invention can be specifically bound with the A-beta oligomer, effectively inhibit the fibrosis aggregation of A-beta and obviously alleviate the toxic effect of the A-beta on cells. The invention also relates to a pharmaceutical composite containing the antibody. The antibody of the invention has strong activity, good specificity, easy preparation and wide prospect of experiment application and clinical application.
Owner:TSINGHUA UNIV

Advanced photoresist process quality evaluation method and system

The invention discloses an advanced photoresist process quality evaluation method and system, wherein the advanced photoresist process quality evaluation system comprises an image acquisition module,a contour extraction module, a roughness calculation module, a fitting module, a feature analysis module and a quality evaluation module, wherein the image acquisition module, the contour extraction module, the roughness calculation module, the fitting module, the feature analysis module and the quality evaluation module are connected in sequence to photoetch the acquired photoresist to obtain anelectron beam microscopic image; according to the electron beam microscopic image, extracting the photoresist edge contour, and calculating the edge roughness and the width roughness at the same time;and calculating a power spectral density curve by utilizing a fitting function, obtaining fitting parameters, calculating characteristic parameters of the electron beam microscopic image, and establishing a photoresist quality evaluation table according to the characteristic value of the photoresist, so that an optimal photoresist product can be screened.
Owner:NANJING CHENGXIN INTEGRATED CIRCUIT TECH RES INST CO LTD

H9N2 type flu virus-like particle vaccine for prevention and preparation method thereof

The invention provides an H9N2 type flu virus-like particle vaccine for prevention and a preparation method thereof. The vaccine composition comprises H9N2 type flu virus-like particles, the virus-like particles are of the virus-like shell particle structure of the H9N2 type flu virus, and the shell particle structure capsule membrane surfaces contain one or more structural protein of the H9N2 type flu virus; the preparation method includes the steps that firstly, HA, NA and M1 gene DNA are synthesized, recombinant plasmid pFastBac-DuaL-HA-NA-M1 is constructed, the constructed recombinant plasmid is led into E.coLi competent state cell strain DH10Bac cells, the plasmid rBacmid-HA-NA-M1 is obtained through blue-white selection, transfection of SF9 insect cells is conducted, the virus-like particles are obtained, an adjuvant is dissolved in the virus-like particles, and the vaccine is obtained after incubation and centrifugal cleaning are obtained. By means of the H9N2 type flu virus-like particle vaccine, an organism can be stimulated to generate a strong immune response, and immunogenicity is high.
Owner:CHONGQING UNIV OF TECH +1

PiggyBac transposon mediated transgenic vector for inducing cell immortalization, its construction method and its application

The invention relates to a PiggyBac transposon mediated transgenic vector for inducing cell immortalization, a construction method and an application, which belongs to the gene engineering technical field. The vector is PB-rtTA-NIT-STP, which is constructed by the following method: constructing a LoxP-SV40P nco-IRES-tk-PolyA-LoxP gene expression cassette; connecting an amplified fragment of the gene expression cassette, constructing a basic transgenic vector PB-NIT; constructing a transgenic vector PB-NIT-STP containing target gene SV40T and p 53; and constructing a screening vector PB-rtTA-NIT-STP containing Tet binding domain rtTA. The transgenic vector constructed by the invention has the advantage of effectively raising the transgene efficiency, and is convenient for screening.
Owner:NORTHWEST A & F UNIV

Human lung cell BEAS-2B/CYP2A13, and preparation method and application thereof

The invention relates to a human recombination gene lung epithelial cell, in particular to a human lung cell BEAS-2B / CYP2A13 and application thereof. The human lung cell BEAS-2B / CYP2A13 is characterized in that: the cell strain is collected in China Center for Type Culture Collection, and the collection number is CCTCC No: C2010127. In the method, the BEAS-2B cell for stably expressing CYP2A13 is constructed by utilizing a slow virus system for the first time at home and abroad, which is the key technology in the invention. Compared with other virus vector systems, the technology has the advantages of high transfection efficiency, stable expression, easiness in screening and the like, and has the unique advantage on transfection of immortalized cells.
Owner:NANJING MEDICAL UNIV

Gene engineering monoclonal antibody combined with A-beta oligomer specificity

The invention relates to the technical field of engineered antibody and provides a monoclonal antibody. The variable region of heavy chain of the monoclonal antibody contains the amino acid sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEO ID NO.3; the variable region of light chain of the monoclonal antibody contains the amino acid sequences shown in SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The invention also specifically provides a humanization single-chain antibody generated by the recombinant strain of enterotoxigenic Escherichia coli with the accession number CGMCC No.2820 and the amino acid sequence of the humanization single-chain antibody is shown in SEQ ID NO.7. The antibody of the invention can be specifically bound with A-beta oligomer, effectively inhibit the fibrosis aggregation of A-beta and obviously alleviate the toxic effect of the A-beta on cells. The invention also relates to a pharmaceutical composite containing the antibody. The antibody of the invention has strong activity, good specificity, easy preparation and wide prospect of experiment application and clinical application.
Owner:TSINGHUA UNIV

Intestinal trefoil factor recombinant expression vector and preparation method of intestinal trefoil factor

The invention discloses a process which uses pGAP as a promoter and Pichia Yeast GS115 as an engineering bacterium to perform fermentation expression on an intestinal trefoil factor (ITF) in a fermentation tank. The method comprises the following steps: the recombinant Pichia Yeast expression vector pGAPZ alpha A-ITF is firstly constructed and then recombined in Escherichia coli Top10 to amplify, Escherichia coli plasmid is extracted and linearized to be transformed in Saccharomyce GS115, a high copy transformant is screened according to the zeocin resistance gene carried by the vector to perform constitutive expression, a high expression pilot test engineering bacterium is screened and fermented in a culture medium for 2 days to perform secretory expression on the ITF; and the target protein ITF of which the purity is more than 95% is obtained through purification treatment, wherein the output is about 200mg / L and the yield is about 50%. The intestinal trefoil factor recombinant expression vector has low production cost, short fermentation period and simple purification method; methanol induction is not adopted, the protein expression level is high; and a good foundation is laid for the large-scale industrial production of ITF.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA

Method for repaid rejuvenation of flammulina velutipes degraded strain

The invention relates to a method for repaid rejuvenation of a flammulina velutipes degraded strain. The method includes steps: degraded strain transferring, fruiting body induction, rejuvenated strain acquisition and rejuvenated strain detection. The rejuvenation operation is performed on the flammulina velutipes degraded strain, an obtained rejuvenated strain is subjected to cultivation comparison with the degraded strain, and the rejuvenated strain which recovers original agronomic characters or is superior in character is a successfully rejuvenated flammulina velutipes strain. The rejuvenation method is provided for the flammulina velutipes degraded strain and is convenient and simple in operation, short in rejuvenation period, low in cost, less prone to contamination and applicable torapid purification and rejuvenation of the flammulina velutipes degraded strain, and the rejuvenated strain has excellent characters.
Owner:INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI

Method for repairing cultivation soil by using semi-matrix covering

The invention discloses a method for repairing cultivation soil by using a semi-matrix covering, and belongs to the field of agricultural planting. The method comprises the following steps that S1, surface soil of a cultivation tank is pretreated, wherein the soil 20-30 cm away from the ground is turned to obtain the pre-treated surface soil; S2, deep treatment of the surface soil is conducted, wherein a disinfectant is added to the filtered surface soil; S3, the cultivation tank is repaired and maintained, and the deep-treated surface soil is placed into the cultivation tank; S4, the semi-matrix covering is added, wherein the semi-matrix covering covers the surface of the cultivation tank; S5, data counting is performed, and repaired nutrient soil is obtained when the parameters of a germination test plant meet the normal plant standards. Through disinfectant application (a chemical modifier method), soil deep ploughing (a soil replacement method) and semi-matrix covering (a bioremediation method), multi-layer soil repairing is conducted, cultivation semi-soil semi-matrix repairing is conducted, the occurrence of disease and pest damage is reduced, the dead rate of plants is reduced and accordingly the annual yield is improved.
Owner:海口路氏肥业有限公司

Technology for converting unformatted data into formatted data in web website

The invention discloses technology for converting unformatted data into formatted data in a web website. The technology comprises the following steps of: establishing a webpage content aggregation service, and capturing the specified webpage; then establishing a website intelligent resolution service and a website semantic pattern base; and purifying heterogeneous website data to obtain standard formatted data. The technology greatly reduces the cost of enterprises and improves the reliability of webpage information transplanting by uniformly converting multiple kinds of heterogeneous information in the web website into the formatted data which is convenient to store and is easy to screen.
Owner:北京博越世纪科技有限公司

Application of serum exosome miRNAs marker to early diagnosis of endemic arsenism

The invention belongs to the field of gene engineering and clinical medicine and particularly relates to an endemic arsenism early diagnosis associated serum exosome miRNAs marker and application thereof. The marker is a combination of has-miR-155 and has-miR-191. The marker and a primer thereof can be used for preparation of a kit for early diagnosis of endemic arsenism.
Owner:NANJING MEDICAL UNIV

Dairy packaging cup hole detection structure

The invention discloses a dairy packaging cup hole detection structure which comprises a water channel and a transfer bar passing the water channel from above. A plurality of driving devices with downward driving ends are fixed on the transfer bar, the driving end of each driving device is fixedly connected with a clamping device, the bottom of each clamping device is provided with a water sensor, the center position of each clamping device is provided with a weighing sensor, one small ball is hung on the bottom of each of the clamping devices; each driving device is provided with a controller and a warning light, the receiving end of the controller is electrically connected with the water sensor and the weighing sensor, and the control end of the controller is electrically connected to the corresponding driving device and the warning light. By the arrangement, the problem that breakage of a dairy package cup cannot be discovered through naked eyes before filling in the prior art can be solved, and the purpose that whether a packaged cup is broken or not before use is detected is realized.
Owner:欧德机械成都有限公司

Recombinant estrogen receptor fluorescent two-hybrid yeast as well as preparation method and application

InactiveCN104498376ABypass the limitation of low sensitivityEasy to operateFungiFusion with DNA-binding domainFluorescenceGenotype
The invention discloses recombinant estrogen receptor fluorescent two-hybrid yeast as well as a preparation method and an application thereof. The invention discloses recombinant estrogen receptor fluorescent two-hybrid yeast, which is obtained by introducing the encoding gene of fusion protein containing an estrogen receptor and a DNA combination domain and the encoding gene of fusion protein containing an estrogen receptor nuclear receptor coactivator and a transcription activating domain into fluorescent yeast Yluc with genotype of MATalpha, ura 3-5, ade 2-101 trp 1-901, leu 2-3, 112, gal4detla, met-, gal80 delta, URA::GALiUAS-GAL1TATA-Luc. The yeast disclosed by the invention is stable in gene character, is easy to culture and screen and can be used for detecting xenoestrogen compounds or estrogenic compounds in environment, and the detection method is simple, convenient and quick and can be suitable for the requirements of high-throughput quick detection.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI

Method for obtaining transgenic cotton through transformation of live immature embryos mediated by Agrobacterium

The invention discloses a method for obtaining transgenic cotton by transforming live immature embryos mediated by Agrobacterium, which consists of the steps of peeling off aseptic embryos, preparing transformation liquid, shaking and infecting, preparing transgenic cotton, and identifying transgenic plants. In the aseptic embryo stripping, 30% hydrogen peroxide and sterile water are prepared into a disinfectant solution, the cotton seeds are plucked and put into the disinfectant solution, soaked at 25°C for 24 hours, the seeds are taken out and placed in a petri dish, and the seed coat and 1 / 3 to 1 / 2 cotyledons, and the remaining cotton embryos are placed in a petri dish containing basal medium to injure the cotton embryos; in the step of preparing the transformation solution, the induction solution is made of acetosyringone and magnesium sulfate, and acetosyringone Ketones can induce the activation of the Vir gene of Agrobacterium and improve the efficiency of exogenous gene integration; in the shaking infection step, Silwet L‑77 and MS liquid medium were added to the transformation solution to make Agrobacterium cover cotton embryos in a wider range. The invention has the advantages of easy screening, short cotton growth cycle, easy operation, transformation efficiency and the like.
Owner:SHAANXI NORMAL UNIV

Composition for digesting allelochemicals in semi-substrate covering raw materials and using method of composition,

The invention discloses a composition for digesting allelochemicals in semi-substrate covering raw materials and a using method of the composition, and belongs to the field of garden engineering. Thecomposition comprises ground bark particles and a broad-spectrum microorganism combined composting substance. The using method comprises the following steps: S1, nutrient soil is laid on a bottom layer, and plant fibers, a nutrient substance and an original bacterium solution are sequentially added on the surface of the nutrient soil from top to bottom; S2, operators gather the nutrient soil and the composition into a long strip-shaped gathering pile with a width of 1-2.5 m and a height of 1.5-2 m, meanwhile, air-permeable coverage is carried out on the surface of the gathered object, and semi-corrosion treatment is carried out; S3, the operators control the semi-corrosion treatment temperature to be 43-52 DEG C; and S4, the operators replace the surface of the gathered object by a closedcover, decomposition treatment is carried out, and the temperature is controlled to be 60-65 DEG C in the decomposition process. According to the technical scheme, broad-spectrum microorganisms are used for decomposition, so that tree bark is prevented from generating an uncoupling agent, and the damage to plant roots is avoided.
Owner:海口路氏肥业有限公司

Genetically engineered monoclonal antibody specifically binding to a-beta oligomer

The present invention relates to the technical field of genetically engineered antibodies, and provides a monoclonal antibody whose heavy chain variable region contains the amino acid sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEO ID NO.3, and the light chain variable region The region contains the amino acid sequences shown in SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The present invention also specifically provides a humanized single-chain antibody produced by a genetically engineered strain of Escherichia coli with a deposit number of CGMCC No. 2821, and its amino acid sequence is shown in SEQ ID NO.7. The antibody of the invention can specifically bind to the A-beta oligomer, effectively inhibit the fibrosis aggregation of A-beta, and obviously reduce the cytotoxic effect of A-beta on cells. The invention also relates to a pharmaceutical composition containing the antibody. The antibody of the invention has strong activity, good specificity, is easy to prepare, and has broad experimental application and clinical application prospects.
Owner:TSINGHUA UNIV

Tobacco NtMLO1 and NtMLO2 fixed-point co-knockout system and application thereof

The invention belongs to the field of plant genetic engineering, and particularly relates to a tobacco NtMLO1 and NtMLO2 fixed-point co-knockout system and application thereof. The invention discloses a homologous sequence of tobacco genes NtMLO1 and NtMLO2, a target sequence is designed based on the homologous sequence, a tobacco NtMLO1 and NtMLO2 fixed-point co-knockout system is constructed, the tobacco NtMLO1 and NtMLO2 genes can be knocked out at the same time, and the simultaneous mutation efficiency of the two genes is 50.0%. The invention provides an efficient breeding mode for creating powdery mildew resistant germplasm resources of tobacco and cultivating powdery mildew resistant tobacco varieties.
Owner:TOBACCO RES INST CHIN AGRI SCI ACAD

Film sealing tooling for lead-acid battery liquid storage bottle

The invention discloses a film sealing tooling for a lead-acid battery liquid storage bottle, and belongs to the technical field of ammunition fuze products. The film sealing tooling is composed of abase (1), a cover plate (2), a welding head (4) and a modified welding head (5), wherein the modified welding head (5) is formed by processing waste universal welding heads into threads for modification. The liquid storage bottle (3) is put into the hole of the base (1) and the liquid storage bottle is fixed through the cover plate (2). A PET / PE composite film is coated on the mouth of the liquidstorage bottle, the PE surface of the film is heated and sealed with the mouth of the bottle by the welding head (4) and the excess film of the mouth of the bottle is taken out and cut off. The problems of low qualified rate and low efficiency in preparing the lead-acid battery liquid storage bottle can be solved and the screening process of the liquid storage bottle can be simplified and the screening period thereof can be shortened.
Owner:CHANGAN AUTOMOBILE (GRP) CO LTD

A method for screening flavored milk powder formula

The invention relates to the technical field of dairy products, and discloses a method for screening flavored milk powder formulas. The method of the present invention simulates the production process to design the rapid viscosity analyzer (RVA) measuring program of the common modulated milk powder formula, and tests the viscosity change of the common modulated milk powder feed liquid in the simulated production heating process; simultaneously monitors the actual production with the same formula product The viscosity of the thick milk before spraying at the control point was compared, and the consistency of the viscosity data confirmed that the method was feasible, so as to determine the RVA measurement procedure of the flavored milk powder, and then screen the flavored milk powder formula according to the characteristic value of the viscosity curve. The method of the invention is easy to operate, and the characteristic value of the viscosity curve is clear. As the basis for judging the flavored milk powder formula, it is concise and clear, easy to judge, and can quickly screen the formula. And it effectively reduces the impact caused by the deviation of production experience, reduces the unfavorable loss caused by trial production, and reduces the cost and time of research and development of new flavored milk powder.
Owner:贝因美(杭州)食品研究院有限公司

Photoinduced gene promoter, recombinant vector, construction method of recombinant vector and recombinant bacteria

The invention discloses a photoinduced gene promoter, a recombinant vector, a construction method of the recombinant vector and recombinant bacteria. The recombinant vector comprises the photoinduced gene promoter, and the nucleotide sequence of the photoinduced gene promoter is shown as SEQ ID NO.1. According to the invention, the photoinduced gene promoter is used for replacing a constitutive gene promoter, a recombinant vector containing a specific photoinduced promoter is provided, and the recombinant vector is formed by using pMDC162 plasmid fused with a GUS reporter gene as a basic vector and using the photoinduced gene promoter NTP2 as a target sequence. The recombinant vector constructed by the invention has the advantages of good specificity, efficient and stable expression, easiness for screening and the like, a transgenic material for photoinduced expression of a target gene can be rapidly obtained completely on the basis of ensuring illumination, and the recombinant vector is expected to play an important role in regulation and control of expression of the target gene.
Owner:SHENZHEN UNIV +1

Semi-finished product of O-shaped ring with inner diameter smaller than 0.5 mm, forming die and trimming method thereof

The invention discloses a semi-finished product of an O-shaped ring with an inner diameter smaller than 0.5 mm, a forming die and a trimming method thereof, and belongs to the field of trimming of rubber products. The semi-finished product of the O-shaped ring with the inner diameter smaller than 0.5 mm comprises the O-shaped ring, wherein a lug boss is arranged on the cutting edge of the inner circle of the O-shaped ring; a diamond-shaped cutting edge is arranged on the cutting edge of the outer circle of the O-shaped ring; the diamond-shaped cutting edges of the outer circles of adjacent O-shaped rings are overlapped with each other, so that the semi-finished products are connected to form a netlike structure. The cavity of the forming die corresponds to the structure of the semi-finished product of the O-shaped ring. The trimming method comprises the steps of: (1) charging; (2) spraying a lubricant; (3) trimming centrifugally; (4) sorting. The method realizes the purpose of reducing trimming length of the O-shaped ring with the inner diameter smaller than 0.5 mm, improving the sealing performance of the O-shaped ring, and reducing the dynamic sealing friction resistance.
Owner:南京金三力橡塑有限公司

Double-cross yeast gER beta 1-GRIP1 for reorganizing estrogen receptor of gobiocypris rarus and application of double-cross yeast gER beta 1-GRIP1

The invention discloses a double-cross yeast gER beta 1-GRIP1 for reorganizing an estrogen receptor of gobiocypris rarus and application of the double-cross yeast gER beta 1-GRIP1. The invention discloses double-cross yeast. The double-cross yeast is obtained by transferring a coding gene of fusion protein of a ligand binding region and a DNA bonding structure region containing the estrogen receptor beta 1 of the gobiocypris rarus and a coding gene of fusion protein of a common activity factor and a copying activity structure region containing the estrogen receptor into fluorescent yeast of which the gene type is MAT alpha, ura 3-5, ade 2-101 trp 1-901, leu2-3, 112, gal4 delta, met-, gal80 delta, URA:: GAL1UAS-GAL1TATA-Luc. The double-cross yeast gER beta 1-GRIP1 has important application in detecting the toxic equivalent or the size of potential ecological toxicity in natural estrogen and / or similar estrogen compounds in environmental samples, in particular assessing the risk which may exist in the endocrine system of the gobiocypris rarus.
Owner:RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI

Terminal-based flow control system and method

InactiveCN112215649ARealize refined requirementsScreenedAdvertisementsResourcesEmbedded systemServer-side
A terminal-based flow control system is located at a server side and comprises a connection module, a sorting module and a loading module. The connecting module is used for connecting a plurality of advertisement putting platforms; the sorting module is used for dynamically sorting the advertisements of the different advertisement putting platforms, so that the different advertisement putting platforms have the same display opportunity; and the loading module is used for sending the advertisements to the terminal for display according to the sorting of the sorting module. The system can control the flow of each terminal to maintain the same level, and achieves transverse comparison of each flow effect.
Owner:上海酷量科技有限公司
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