38results about How to "Screened" patented technology

The invention provides an H9N2 type flu virus-like particle vaccine for prevention and a preparation method thereof. The vaccine composition comprises H9N2 type flu virus-like particles, the virus-like particles are of the virus-like shell particle structure of the H9N2 type flu virus, and the shell particle structure capsule membrane surfaces contain one or more structural protein of the H9N2 type flu virus; the preparation method includes the steps that firstly, HA, NA and M1 gene DNA are synthesized, recombinant plasmid pFastBac-DuaL-HA-NA-M1 is constructed, the constructed recombinant plasmid is led into E.coLi competent state cell strain DH10Bac cells, the plasmid rBacmid-HA-NA-M1 is obtained through blue-white selection, transfection of SF9 insect cells is conducted, the virus-like particles are obtained, an adjuvant is dissolved in the virus-like particles, and the vaccine is obtained after incubation and centrifugal cleaning are obtained. By means of the H9N2 type flu virus-like particle vaccine, an organism can be stimulated to generate a strong immune response, and immunogenicity is high.

The invention relates to a PiggyBac transposon mediated transgenic vector for inducing cell immortalization, a construction method and an application, which belongs to the gene engineering technical field. The vector is PB-rtTA-NIT-STP, which is constructed by the following method: constructing a LoxP-SV40P nco-IRES-tk-PolyA-LoxP gene expression cassette; connecting an amplified fragment of the gene expression cassette, constructing a basic transgenic vector PB-NIT; constructing a transgenic vector PB-NIT-STP containing target gene SV40T and p 53; and constructing a screening vector PB-rtTA-NIT-STP containing Tet binding domain rtTA. The transgenic vector constructed by the invention has the advantage of effectively raising the transgene efficiency, and is convenient for screening.

The invention relates to a human recombination gene lung epithelial cell, in particular to a human lung cell BEAS-2B/CYP2A13 and application thereof. The human lung cell BEAS-2B/CYP2A13 is characterized in that: the cell strain is collected in China Center for Type Culture Collection, and the collection number is CCTCC No: C2010127. In the method, the BEAS-2B cell for stably expressing CYP2A13 is constructed by utilizing a slow virus system for the first time at home and abroad, which is the key technology in the invention. Compared with other virus vector systems, the technology has the advantages of high transfection efficiency, stable expression, easiness in screening and the like, and has the unique advantage on transfection of immortalized cells.

The invention relates to the technical field of engineered antibody and provides a monoclonal antibody. The variable region of heavy chain of the monoclonal antibody contains the amino acid sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEO ID NO.3; the variable region of light chain of the monoclonal antibody contains the amino acid sequences shown in SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The invention also specifically provides a humanization single-chain antibody generated by the recombinant strain of enterotoxigenic Escherichia coli with the accession number CGMCC No.2820 and the amino acid sequence of the humanization single-chain antibody is shown in SEQ ID NO.7. The antibody of the invention can be specifically bound with A-beta oligomer, effectively inhibit the fibrosis aggregation of A-beta and obviously alleviate the toxic effect of the A-beta on cells. The invention also relates to a pharmaceutical composite containing the antibody. The antibody of the invention has strong activity, good specificity, easy preparation and wide prospect of experiment application and clinical application.

The invention discloses a process which uses pGAP as a promoter and Pichia Yeast GS115 as an engineering bacterium to perform fermentation expression on an intestinal trefoil factor (ITF) in a fermentation tank. The method comprises the following steps: the recombinant Pichia Yeast expression vector pGAPZ alpha A-ITF is firstly constructed and then recombined in Escherichia coli Top10 to amplify, Escherichia coli plasmid is extracted and linearized to be transformed in Saccharomyce GS115, a high copy transformant is screened according to the zeocin resistance gene carried by the vector to perform constitutive expression, a high expression pilot test engineering bacterium is screened and fermented in a culture medium for 2 days to perform secretory expression on the ITF; and the target protein ITF of which the purity is more than 95% is obtained through purification treatment, wherein the output is about 200mg/L and the yield is about 50%. The intestinal trefoil factor recombinant expression vector has low production cost, short fermentation period and simple purification method; methanol induction is not adopted, the protein expression level is high; and a good foundation is laid for the large-scale industrial production of ITF.

The invention discloses a method for repairing cultivation soil by using a semi-matrix covering, and belongs to the field of agricultural planting. The method comprises the following steps that S1, surface soil of a cultivation tank is pretreated, wherein the soil 20-30 cm away from the ground is turned to obtain the pre-treated surface soil; S2, deep treatment of the surface soil is conducted, wherein a disinfectant is added to the filtered surface soil; S3, the cultivation tank is repaired and maintained, and the deep-treated surface soil is placed into the cultivation tank; S4, the semi-matrix covering is added, wherein the semi-matrix covering covers the surface of the cultivation tank; S5, data counting is performed, and repaired nutrient soil is obtained when the parameters of a germination test plant meet the normal plant standards. Through disinfectant application (a chemical modifier method), soil deep ploughing (a soil replacement method) and semi-matrix covering (a bioremediation method), multi-layer soil repairing is conducted, cultivation semi-soil semi-matrix repairing is conducted, the occurrence of disease and pest damage is reduced, the dead rate of plants is reduced and accordingly the annual yield is improved.

The invention discloses technology for converting unformatted data into formatted data in a web website. The technology comprises the following steps of: establishing a webpage content aggregation service, and capturing the specified webpage; then establishing a website intelligent resolution service and a website semantic pattern base; and purifying heterogeneous website data to obtain standard formatted data. The technology greatly reduces the cost of enterprises and improves the reliability of webpage information transplanting by uniformly converting multiple kinds of heterogeneous information in the web website into the formatted data which is convenient to store and is easy to screen.

The invention discloses a dairy packaging cup hole detection structure which comprises a water channel and a transfer bar passing the water channel from above. A plurality of driving devices with downward driving ends are fixed on the transfer bar, the driving end of each driving device is fixedly connected with a clamping device, the bottom of each clamping device is provided with a water sensor, the center position of each clamping device is provided with a weighing sensor, one small ball is hung on the bottom of each of the clamping devices; each driving device is provided with a controller and a warning light, the receiving end of the controller is electrically connected with the water sensor and the weighing sensor, and the control end of the controller is electrically connected to the corresponding driving device and the warning light. By the arrangement, the problem that breakage of a dairy package cup cannot be discovered through naked eyes before filling in the prior art can be solved, and the purpose that whether a packaged cup is broken or not before use is detected is realized.

The invention discloses recombinant estrogen receptor fluorescent two-hybrid yeast as well as a preparation method and an application thereof. The invention discloses recombinant estrogen receptor fluorescent two-hybrid yeast, which is obtained by introducing the encoding gene of fusion protein containing an estrogen receptor and a DNA combination domain and the encoding gene of fusion protein containing an estrogen receptor nuclear receptor coactivator and a transcription activating domain into fluorescent yeast Yluc with genotype of MATalpha, ura 3-5, ade 2-101 trp 1-901, leu 2-3, 112, gal4detla, met-, gal80 delta, URA::GALiUAS-GAL1TATA-Luc. The yeast disclosed by the invention is stable in gene character, is easy to culture and screen and can be used for detecting xenoestrogen compounds or estrogenic compounds in environment, and the detection method is simple, convenient and quick and can be suitable for the requirements of high-throughput quick detection.

The invention discloses a film sealing tooling for a lead-acid battery liquid storage bottle, and belongs to the technical field of ammunition fuze products. The film sealing tooling is composed of abase (1), a cover plate (2), a welding head (4) and a modified welding head (5), wherein the modified welding head (5) is formed by processing waste universal welding heads into threads for modification. The liquid storage bottle (3) is put into the hole of the base (1) and the liquid storage bottle is fixed through the cover plate (2). A PET/PE composite film is coated on the mouth of the liquidstorage bottle, the PE surface of the film is heated and sealed with the mouth of the bottle by the welding head (4) and the excess film of the mouth of the bottle is taken out and cut off. The problems of low qualified rate and low efficiency in preparing the lead-acid battery liquid storage bottle can be solved and the screening process of the liquid storage bottle can be simplified and the screening period thereof can be shortened.

The invention discloses a photoinduced gene promoter, a recombinant vector, a construction method of the recombinant vector and recombinant bacteria. The recombinant vector comprises the photoinduced gene promoter, and the nucleotide sequence of the photoinduced gene promoter is shown as SEQ ID NO.1. According to the invention, the photoinduced gene promoter is used for replacing a constitutive gene promoter, a recombinant vector containing a specific photoinduced promoter is provided, and the recombinant vector is formed by using pMDC162 plasmid fused with a GUS reporter gene as a basic vector and using the photoinduced gene promoter NTP2 as a target sequence. The recombinant vector constructed by the invention has the advantages of good specificity, efficient and stable expression, easiness for screening and the like, a transgenic material for photoinduced expression of a target gene can be rapidly obtained completely on the basis of ensuring illumination, and the recombinant vector is expected to play an important role in regulation and control of expression of the target gene.

A terminal-based flow control system is located at a server side and comprises a connection module, a sorting module and a loading module. The connecting module is used for connecting a plurality of advertisement putting platforms; the sorting module is used for dynamically sorting the advertisements of the different advertisement putting platforms, so that the different advertisement putting platforms have the same display opportunity; and the loading module is used for sending the advertisements to the terminal for display according to the sorting of the sorting module. The system can control the flow of each terminal to maintain the same level, and achieves transverse comparison of each flow effect.