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Method for repaid rejuvenation of flammulina velutipes degraded strain

A technology of Flammulina velutipes and strains, which is applied in the field of rapid rejuvenation of degenerated strains of Flammulina velutipes, can solve the problems of heavy workload and technical complexity in the identification and screening of single-cell cultures, achieve good application prospects, good repeatability, and shorten the formation cycle Effect

Active Publication Date: 2019-07-05
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is technically complex, and has disadvantages such as a large workload for identification and screening of single-cell cultures in the later stage, and the excellent traits of different single-cell cultures are accidental.

Method used

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  • Method for repaid rejuvenation of flammulina velutipes degraded strain
  • Method for repaid rejuvenation of flammulina velutipes degraded strain
  • Method for repaid rejuvenation of flammulina velutipes degraded strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of rapid rejuvenation method of Flammulina velutipes degenerate strain, comprises the following steps:

[0038] Transfer of degenerate strains: Add sterilized rejuvenation medium into a standard aseptic culture dish with a diameter of 90mm to make a medium plate with a thickness of 7mm, and pick 0.3cm 2 The mycelia of the degenerated strains of the degenerated strains were inoculated on the rejuvenation medium plate, and the inoculated medium plate was sealed with a sealing film for one layer, then placed in a constant temperature incubator, and cultured in the dark at 22°C for 9 days, and the mycelium was obtained. Plate colonies;

[0039] The rejuvenation medium is composed of: 200 g of potatoes, 20 g of glucose, 1 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 100 g of corncobs, 100 g of wheat bran, 3 g of soybean protein concentrate, 5 μg of anthranilic acid, 50 μg of riboflavin, agar 20g, add water to make up to 1000mL.

[0040] Fruiting ...

Embodiment 2

[0046] A kind of rapid rejuvenation method of Flammulina velutipes degenerate strain, comprises the following steps:

[0047] Transfer of degenerate strains: Add sterilized rejuvenation medium into a standard sterile culture dish with a diameter of 90 mm to make a medium plate with a thickness of 8 mm, and pick 0.2 cm 2 The mycelia of the degenerated strains of the degenerate strains were inoculated on the rejuvenation medium plate, and the inoculated medium plate was sealed with a sealing film for 2 layers, then placed in a constant temperature incubator, and cultured in the dark at 22°C for 9 days, and the mycelium was obtained. Plate colonies;

[0048] The rejuvenation medium is composed of: 200 g of potatoes, 20 g of glucose, 1 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 80 g of corncobs, 80 g of wheat bran, 3 g of soybean protein concentrate, 5 μg of anthranilic acid, 50 μg of riboflavin, and agar 20g, water 1000mL.

[0049] Fruiting body induction: u...

experiment example 1

[0071] The rejuvenated strains and degenerated strains of Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 were subjected to cultivation tests.

[0072] Cultivation is carried out in a factory-style bottle planting method, liquid strains are inoculated, and after inoculation, they are cultivated in the dark, at 17°C, and at a relative air humidity of 60-65%. After the mycelium is full, use the fungus scratching machine to scratch the fungus, inject water and move it into the fruiting room. The management of mushroom production is the same as that of conventional factories. Harvest when the fruiting body grows to 14-15cm and the cap is about to open, and measure the biological efficiency of different bacterial strains.

[0073] The fruiting bodies of Flammulina velutipes cultivated in embodiment 1 (such as image 3 shown) compared with the fruiting bodies of Flammulina velutipes cultivated by degraded strains (such as figure 2 Shown) and the...

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Abstract

The invention relates to a method for repaid rejuvenation of a flammulina velutipes degraded strain. The method includes steps: degraded strain transferring, fruiting body induction, rejuvenated strain acquisition and rejuvenated strain detection. The rejuvenation operation is performed on the flammulina velutipes degraded strain, an obtained rejuvenated strain is subjected to cultivation comparison with the degraded strain, and the rejuvenated strain which recovers original agronomic characters or is superior in character is a successfully rejuvenated flammulina velutipes strain. The rejuvenation method is provided for the flammulina velutipes degraded strain and is convenient and simple in operation, short in rejuvenation period, low in cost, less prone to contamination and applicable torapid purification and rejuvenation of the flammulina velutipes degraded strain, and the rejuvenated strain has excellent characters.

Description

technical field [0001] The invention belongs to the technical field of Flammulina velutipes strains, in particular to a rapid rejuvenation method of Flammulina velutipes degenerated strains. Background technique [0002] Flammulina velutipes (Fr.) Sing) formal name used at school is hair handle money fungus, also known as hair handle small fire mushroom, structure fungus, port mushroom, winter mushroom etc. Flammulina velutipes is one of the main mushroom species cultivated in my country, and it is also the mushroom species with the largest scale of industrial production in my country. Its taste is crisp and tender, and it is rich in nutrition, which is deeply loved by consumers. Strain is the basis of edible fungus production, which is directly related to the yield, quality and benefit of the final product. At present, during the continuous passage of Flammulina velutipes production strains in PDA medium, problems such as aging and degradation of strain activity will occur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12R1/645
CPCC12N1/14C12N1/38
Inventor 黄春燕宫志远谢红艳李瑾杨鹏韩建东姚强
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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