63 results about "Anthranilic acid" patented technology

The invention discloses recombinant escherichia coli capable of increasing the yield of L-tryptophan and application of the recombinant escherichia coli. According to the invention, o-aminobenzoic acid synthase TrpEQ71K and 3-deoxy-D-arabinoheptanoic acid-7-phosphate synthase (DAHP synthase) AroGQ151F, which are used for solving feedback, are subjected to integrated expression on a genome of the L-tryptophan production strain TRP30, so that the constructed engineering strain TRP30 can be used for remarkably improving the yield of the L-tryptophan. After the strain is cultured in a 5L fermentation tank for 48h, the determination shows that the yield of the L-tryptophan is increased to 52.1 g / L, which is increased by 22.59% compared with the yield of the L-tryptophan of a control strain.

The invention relates to the technical field of microbial metabolism engineering, and discloses a genetically engineered bacterium for high-yield L-histidine as well as a construction method and application of the genetically engineered bacterium. A nucleoside triphosphate pyrophosphate hydrolase coding gene mazG gene and a flagellin synthesis gene flhC gene are knocked out at the same time, an o-aminobenzoic acid phosphoribosyltransferase coding gene trpD gene and a ribulose phosphate 3-isomerase coding gene rpe gene are knocked down, and the recombinant protein is obtained. A ribose-5-phosphate isomerase gene coding rpiA gene and a lysine exponential coding gene lysE gene are overexpressed, and a promoter of 6-phosphoglucose isomerase pgi is replaced by a PfliC promoter, so that key substances for synthesis of L-histidine can be effectively accumulated, the fermentation yield of L-histidine is greatly increased, and compared with an initial strain, the yield of L-histidine is increased by 33.3%.

The invention discloses a composition and a cosmetic with anti-allergy, itching-relieving and redness-removing effects. The composition is prepared from the following raw material components: a herba centellae extract with madecassoside content of not less than 85wt%, a dendrobium officinale stem extract and dihydrooat acyl o-aminobenzoic acid. The composition comprising the three components is added into the cosmetic, so that the cosmetic has good system stability, also has enhanced anti-allergy, itching-relieving and red-removing effects, and can improve skin problems from multiple dimensions.

The invention relates to a method for producing tryptamine. Disclosed herein are prokaryotic and eukaryotic microorganisms, including Escherichia coli and Saccharomyces cerevisiae, genetically altered to biosynthesize tryptamine and tryptamine derivatives. Microorganisms of the present disclosure may be engineered to comprise a plasmid and a stable gene integration containing genetic information sufficient to convert anthranilic acid or indole to tryptamine. Fermentative production of substituted tryptamine in the presence of a whole-cell biocatalyst can be used to economically and efficiently produce these compounds for therapeutic use.

The application belongs to the technical field of medicine, and relates to N-methyl-2-(9-oxoacridin-10(9H)-yl)acetamide compounds and a preparation method and application thereof. The preparation method of the compound is as follows: taking o-aminobenzoic acid as a starting material, performing substitution reaction, condensation reaction, alkylation reaction and hydrolysis reaction to generate a key intermediate acridone acetic acid, and finally performing condensation reaction on the acridone acetic acid and substituted pyrimidine in the presence of TCFH and NMI condensing agents to obtain the target compound. The preparation method of the N-methyl-2-(9-oxoacridin-10(9H)-yl)acetamide compound provided in the application is simple and feasible, and good results are shown in in-vitro anti-tumor activity tests, and the compound has excellent anti-proliferation ability on human breast cancer cells BT-549 and MDA-MB-231 in particular.

The present invention belongs to the technical field of biological medicine, and discloses a ceramide compound shown in Chemical Formula I: #imgabs0# wherein, R 1 is selected from the residues after condensation of sialic acid, chelidamic acid monohydrate, 2,5-furandicarboxylic acid, quinic acid, chlorogenic acid, ectoine, nicotinic acid, shikimic acid, glucuronic acid, salicylic acid, carnosic acid, dihydrooat acylanthranilic acid, abietic acid, ursolic acid, tranexamic acid, syringic acid, gentisic acid, vanillic acid, 5-hydroxyisovanillic acid, orotic acid, 5-aminoorotic acid, 3,5-dihydroxy-4-methylbenzoic acid, glycyrrhizic acid or gallic acid, R 2 is selected from one of the following structures: -C 15 H 29 、-C 15 H 31 、-C 15 H 27 、-CHOHC 14 H 27 、-CHOHC 14 H 29 . The present invention also discloses a preparation method and application of the ceramide compound. The present invention introduces a molecular fragment with physiological activity of a functional carboxylic acid into ceramide, which will enhance the original efficacy of this type of molecule and also improve the physicochemical properties of the ceramide compound.

The application relates to a reagent combination for detecting depression, a manufacturing method, a detection system and application thereof, and belongs to the technical field of instrument detection. The reagent combination for detecting depression comprises a calibrator, a quality control sample and an extraction solution of a to-be-detected compound serving as a biomarker; wherein the to-be-detected compound is at least three kinds selected from 5-hydroxyanthranilic acid, methionine, gamma-aminobutyric acid, 3,4-dihydroxyphenylacetic acid, 2-picolinic acid, quinolinic acid, kynurenine, 5-hydroxytryptamine, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, dopamine and norepinephrine. The reagent combination for detecting depression and the detection system can significantly improve the accuracy of diagnosis, are convenient to detect, have good repeatability of detection data, are high in specificity, and are accurate in clinical diagnosis results.

Disclosed herein are prokaryotic and eukaryotic microbes, including E. coli and S. cerevisiae, genetically altered to biosynthesize tryptamine and tryptamine derivatives. The microbes of the disclosure may be engineered to contain plasmids and stable gene integrations containing sufficient genetic information for conversion of an anthranilate or an indole to a tryptamine. The fermentative production of substituted tryptamines in a whole-cell biocatalyst may be useful for cost effective production of these compounds for therapeutic use.

The invention provides a manganese content determination method which comprises the following steps: dissolving a to-be-determined sample with hydrochloric acid to form a to-be-determined solution; adding phosphoric acid into the solution to be determined; adding perchloric acid into the solution to be measured, and heating; by taking N-benzene o-carbamic acid as an indicator, titrating the to-be-measured solution added with phosphoric acid and perchloric acid by using an ammonium ferrous sulfate standard solution; and determining the content of manganese in the to-be-determined sample according to the dosage of the ammonium ferrous sulfate standard solution. The test method can be used for stably and accurately measuring the manganese content in the sample to be measured.

This invention discloses an optimization device for the ammoniation reaction product in the production of anthranilic acid, comprising a reaction chamber. A second conveying roller is movably connected to the lower left end of the inner surface of the reaction chamber, and a first conveying roller is movably connected to the upper right end of the inner surface of the reaction chamber. The first and second conveying rollers are connected by a conveyor belt. In this invention, raw materials and catalyst are fed into the reaction chamber through the inlet. Turning on the heating plate heats the raw materials and catalyst. Turning on the motor drives the first conveying roller to rotate, which, in conjunction with the conveyor belt, drives the second conveying roller to rotate. This allows the baffles to convey the raw materials and catalyst. Furthermore, the rotation of the second conveying roller drives a second rotating rod to rotate via a single-sided toothed synchronous belt, thereby allowing the stirring blades to stir the raw materials and catalyst, effectively improving the overall reaction efficiency.

The present application relates to the technical field of organic synthesis, and particularly relates to a method for synthesizing 3-methyl-2-phenyl-2,3-dihydrobenzothiazine-4-ketone. A conventional method for synthesizing 3-methyl-2-phenyl-2,3-dihydrobenzothiazine-4-ketone generally uses anthranilic acid as a starting material, and needs to be subjected to six-step continuous reactions to obtain the product, and the reaction time is long, the yield is low, and the operation is complicated. In view of the above problems, the present application provides a method for synthesizing 3-methyl-2-phenyl-2,3-dihydrobenzothiazine-4-ketone, which uses 2-mercapto-N-methyl benzamide as a starting material, and is completed through one-step reaction, and the starting material and catalyst are easy to obtain, the operation is simple, the reaction is fast, the equipment requirement is low, the post-treatment is simple, and the yield is high.

The utility model relates to the production technology field of anthranilic acid, specifically disclose a kind of storage equipment for anthranilic acid production, including storage tank, the bottom of the storage tank inner chamber is equipped with hot plate, the top of the storage tank is equipped with anti-precipitation subassembly, the anti-precipitation subassembly includes frame, the bottom of the frame is fixedly connected with storage tank, the top of the frame is equipped with electric push rod, the output end of the electric push rod is through and extends to the inner chamber of frame and is equipped with push plate. The utility model is transmitted to the storage in storage tank by feed inlet and stores anthranilic acid, when needing to carry out anti-precipitation to anthranilic acid in storage tank, first, anthranilic acid is heated by hot plate, then electric push rod operates, push plate is moved downward by electric push rod, push plate is moved downward by the cooperation of the sliding slot and the sliding block in frame, movable shaft is moved downward by push plate.

The invention relates to an antibacterial and deodorant insole material and a preparation method thereof, and belongs to the technical field of insole materials. The antibacterial and deodorant insole material comprises the following components: EVA, TPU, an antibacterial material, a dispersing agent and a compatilizer, the preparation method comprises the following steps: adding the EVA, the TPU, the antibacterial material, the dispersing agent and the compatilizer into a double-screw extruder, and extruding and granulating to obtain the antibacterial and deodorant insole material. According to the invention, the o-aminobenzoic acid-syringaldehyde composite carrier destroys microbial cell membranes to enhance the sterilization permeation efficiency of nano-silver, and syringaldehyde neutralizes odor molecules to achieve synergistic interaction, so that the integration of dual functions of bacteriostasis and deodorization is realized.

The invention relates to the field of organic synthesis, in particular to a preparation method of 1, 2, 3-benzotriazine-4-ketone, which comprises the following steps: S1, under the catalytic action of a condensing agent T3P, directly carrying out amidation reaction on anthranilic acid and methoxyamine hydrochloride to obtain 2-amino-N-methoxybenzamide; s2, under the condition of a diazotization reaction, the 2-amino-N-methoxybenzamide is converted into 3-methoxy-1, 2, 3-benzotriazine-4-ketone, and the 3-methoxy-1, 2, 3-benzotriazine-4-ketone is converted into 2-methoxy-1, 2, 3-benzotriazine-4-ketone; and S3, reducing the 3-methoxy-1, 2, 3-benzotriazine-4-ketone into the 1, 2, 3-benzotriazine-4-ketone under the condition of palladium on carbon / hydrogen gas, so as to obtain the 1, 2, 3-benzotriazine-4-ketone. The preparation method provided by the invention has the advantages of easily available raw materials, easily separated products and high yield, and is suitable for industrial production.

The invention belongs to the field of pesticides, and discloses a compound with a structure as shown in a formula I. A preparation method of the compound comprises the following steps: reacting anthranilic acid with formamide, and then reacting with 1, 4-dibromobutane under the action of sodium hydride to obtain 3-(4-bromobutyl) quinazoline-4 (3H)-ketone; then, phenylacetic acid sequentially reacts with methyl alcohol, hydrazine hydrate, potassium hydroxide, carbon disulfide and 3-(4-bromobutyl) quinazoline-4 (3H)-ketone, and the quinazolinone derivative containing the oxadiazole unit is obtained. The quinazolinone derivative containing the oxadiazole unit is a novel compound, has an inhibiting effect on fusarium graminearum, rhizoctonia solani and botrytis cinerea, and can be used for preventing and treating plant diseases caused by fungi.

The present invention relates to the preparation of ortho-aminobenzoic acid by means of microbial fermentation, wherein mixtures of glucose and xylose are used as fermentable substrates.

The application belongs to the technical field of biological gene engineering, and particularly relates to a protein for generating methyl o-aminobenzoate, a gene thereof and application. S A protein for generating methyl o-aminobenzoate from adenosylmethionine and o-aminobenzoate, wherein the amino acid sequence of the protein is shown as SEQ ID NO. 2. The experiment of the application proves that methyl o-aminobenzoate methyltransferase CsAAMT is found in a new tea variety 'Zhongchaxiangning', the gene corresponding to the protein is expressed in Escherichia coli, and the purified recombinant protein has the activity of catalyzing S o-aminobenzoate from adenosylmethionine and o-aminobenzoate, and further, the tea leaves without the aroma component can release methyl o-aminobenzoate by transiently overexpressing the gene in tea leaves.

The invention discloses a preparation method and application of an N-phenyl o-aminobenzoic acid rare earth complex, and the preparation method comprises the following steps: optimizing a mixing reaction process, and adopting an accurate dropwise adding device and a constant stirring speed to ensure that a rare earth nitrate solution and an N-phenyl o-aminobenzoic acid ligand solution are uniformly mixed, so as to obtain the N-phenyl o-aminobenzoic acid rare earth complex. The phenomenon of non-uniform mixing caused by direct addition or simple stirring in the prior art is avoided, so that the problems of supersaturation and incomplete reaction of the solution in a local area are avoided; due to the improvement, rare earth ions and ligands uniformly react in the whole solution, the formation of large particles or non-uniform precipitates is remarkably reduced, meanwhile, the uniform and sufficient reaction of all areas is ensured, and the N-phenyl anthranilic acid rare earth complex is finally obtained. In addition, the invention also discloses a new application of the N-phenyl anthranilic acid rare earth complex, and the N-phenyl anthranilic acid rare earth complex can be added into natural rubber as an anti-aging agent of the natural rubber to improve the thermo-oxidative aging resistance of the natural rubber.

The present invention relates to methods for diagnosis and monitoring of food addiction, obesity and related disorders, as well as for the evaluation of treatments directed to said disorders, based on the determination and comparison of Microviridae bacteriophages, preferably Gokushovirus, levels. It also relates to kits and uses for said purposes. Furthermore, the invention relates to anthranilic acid for use in the treatment or prevention of food addiction, obesity and related disorders, and non-therapeutic use in body fat reduction.

The purpose of the invention is to develop, as drug-candidate compounds, a group of novel compounds having the activity of inhibiting functions of Pin1. The invention provides: a compound represented by formula (I) or a salt thereof; and a Pin1 inhibitor, a pharmaceutical composition, a therapeutic or prophylactic agent for inflammatory diseases, a therapeutic or prophylactic agent for cancer, and a therapeutic or prophylactic agent for adiposity that use said compound / salt.

The present invention discloses an enzymatic digestion method for gently reducing agent to digest tissue samples and its mass spectrometry detection method. The present invention provides a lysis solution, which comprises: a detergent with a final concentration of 1-4% by mass fraction; the detergent is sodium deoxycholate and / or ammonium bicarbonate; 3-(2-carboxy)phosphine hydrochloride with a final concentration of 5-20 μM; 5-chloro-anthranilic acid with a final concentration of 20-60 mM; tris(hydroxymethyl)aminomethane-hydrochloride with a final concentration of 50-200 mM; phenylmethylsulfonyl fluoride with a final concentration of 0.1-2 mM; and water. After the tissue samples are lysed with the lysis solution of the present invention, tissue proteins and peptides can be obtained with a high yield.

The present invention relates to the synthesis of dyes. More specifically, the present invention is a method for preparing substituted or unsubstituted indigo, which comprises reacting a substituted or unsubstituted anthranilic acid with glyoxylic acid in a solvent in the presence of a metal catalyst under hydrogenation to obtain a substituted or unsubstituted 2-(carboxymethylamino)benzoic acid, and a step of converting the substituted or unsubstituted 2-(carboxymethylamino)benzoic acid into a substituted or unsubstituted indigo.

The present invention relates to a brewer's yeast and its fermentation product and application, belonging to the technical field of fermentation engineering. The brewer's yeast or its fermentation product can be used in a method for preparing anthranilic acid by biodegrading L-tryptophan, the method has the advantages of fast reaction speed, safe process, green environmental protection, few by-products, convenient downstream extraction and separation, etc., and the obtained anthranilic acid has high yield and high purity.

This invention provides a method for preparing 2,2'-(1,4-phenylene)bis-4H-3,1-benzoxazin-4-one, relating to the field of compounds. The preparation method of this invention includes the following steps: (1) reacting anthranilic acid with terephthaloyl chloride in an organic solvent to generate intermediate A; (2) centrifuging the reaction product of step (1) to separate the wet crystalline intermediate A and the centrifugal mother liquor; (3) performing a cyclization reaction between the wet crystalline intermediate A from step (2) and acetic anhydride; (4) centrifuging, washing, and drying the reaction product of step (3) to obtain the target 2,2'-(1,4-phenylene)bis-4H-3,1-benzoxazin-4-one product. This invention prepares 2,2'-(1,4-phenylene)bis-4H-3,1-benzoxazin-4-one with not only low yellowness values ​​and high purity, but also high yield. The preparation method of the present invention has significant industrial value, and is particularly applicable to the field of polymer material additives where strict requirements are placed on product purity and yellowness.

A composition comprising an active substance selected from nicotinamide; nicotinic acid; nicotinic acid esters; tryptophan; a tryptophan dipeptide; nicotinamide adenine dinucleotide (NAD); nicotinamide adenine dinucleotide phosphate (NADP); an intermediate in the biosynthesis of NAD or NADP selected from the group consisting of N-formylkynurenine, L-kynurenine, 3-hydroxy-L-kynurenine, 3-hydroxyanthranilate, 2-amino-3-carboxymuconate semialdehyde, quinolinate, nicotinic acid mononucleotide (beta-nicotinate D-ribonucleotide), and nicotinic acid adenine dinucleotide; nicotinamide riboside; nicotinamide mononucleotide; 1-methylnicotinamide (N-methylnicotinamide); or a combination thereof, for use for preventing and / or reducing one or more post-acute symptoms of an infection, wherein the composition is formulated to partly or completely release the active substance(s), preferably nicotinamide, for topical supplementation or efficacy in the lower small intestine and / or the colon, wherein preferably the pathogen is SARS-CoV-2 and / or the post-acute symptoms are PCS

The invention relates to a washing and caring composition with dandruff removing, itching relieving and repairing effects and a preparation method of the washing and caring composition, and belongs to the technical field of daily chemicals. Aiming at dandruff formation and itching factors of scalp, the piroctone olamine salt, palmitoyl tetrapeptide-10, tetrapeptide-1 and speranskia herb extract are correspondingly compounded to play a role in removing dandruff and relieving itching, and the limitation of a single component is made up by compounding chemical components and plant extracts, so that more efficient and milder scalp care is realized. In addition, the dihydrooat acyl o-aminobenzoic acid, pyridoxine tripalmitate and quaternary ammonium salt-73 are combined for use, so that the shampoo also has an excellent synergistic repairing capability on scalp with symptoms such as acne, red and swollen, slight folliculitis and the like.

The invention provides application of an NtFAR3 gene in regulation and control of tobacco organic acid metabolism and cultivation of a tobacco cold-sensitive plant, and belongs to the technical field of tobacco gene engineering. Metabolism of tobacco organic acid is regulated by regulating and controlling expression of the NtFAR3 gene, a base sequence of the NtFAR3 gene is shown as SEQ ID NO.1, and the tobacco cold-sensitive plant is constructed by silencing the NtFAR3 gene. In the seedling stage, the contents of anthranilic acid, L-pyroglutamic acid, suberic acid and 4-coumaric acid in the leaves of the NtFAR3 gene silencing plant are obviously increased, and the contents of L-malic acid and 3-hydroxy-3-methylglutamic acid are obviously reduced. According to the invention, a new strategy and path can be provided for cultivating tobacco varieties with different organic acid contents and varieties adapting to different environment temperatures.

The utility model relates to methyl anthranilate production raw material storage equipment, which belongs to the technical field of storage equipment and comprises a storage tank, a stirring shaft, a stirring paddle, a stirring motor, a heat preservation jacket, a liquid inlet pipe, an exhaust pipe, a feeding port, a sealing cover, a discharging pipe, a gas distribution pipe and a nitrogen tank. The other end of the nitrogen conveying pipe extends into the storage tank, an outlet of the nitrogen conveying pipe faces the discharging pipe downwards, the nitrogen conveying pipe is connected with the gas distribution pipe through a pipeline, valves are arranged on the liquid inlet pipe, the exhaust pipe, the discharging pipe and the nitrogen conveying pipe, and a temperature measuring device is arranged in the storage tank. According to the utility model, temporary storage and mixing functions of anthranilic acid and methanol are integrated in one storage tank, so that the number of equipment and the occupied area are reduced, the production cost is reduced, two raw materials are respectively added through the sealed feeding hole and the liquid inlet pipe and are directly mixed in the storage tank, the volatilization and waste of the raw materials in the conveying process are avoided, and the production efficiency is improved. The raw material utilization rate is improved.

The present invention relates to the preparation of cyano-anthranilic acid derivatives from 2-amino-5-formyl-3-methylbenzoic acid derivatives, including 2-amino-5-cyano-3- methylbenzoic acid, in the presence of N-methylpyrrolidone (NMP), N,N- dimethylformamide (DMF), N,N-dimethylacetamide (DMA), dimethylsulfoxide (DMSO), and the mixtures thereof, used as a dehydration agent. The present invention discloses an efficient, high yield process to obtain cyano-anthranilic acid intermediate further leading to the manufacturing of diamides, in particular, cyantraniliprole.