H9N2 type flu virus-like particle vaccine for prevention and preparation method thereof

An influenza virus, virus-like technology, applied in the field of genetic engineering, can solve the problems of human insecurity, high production cost, complex production process, etc., and achieve the effect of good immune effect, safe performance and broad anti-infection spectrum.

Inactive Publication Date: 2016-04-06
CHONGQING UNIV OF TECH +1
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Problems solved by technology

[0004] The current traditional vaccine is mainly used for the infection of chickens, and the existing traditional vaccine is prepared by replicating virus through chicken embryos with isolated infect

Method used

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  • H9N2 type flu virus-like particle vaccine for prevention and preparation method thereof
  • H9N2 type flu virus-like particle vaccine for prevention and preparation method thereof
  • H9N2 type flu virus-like particle vaccine for prevention and preparation method thereof

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[0031] The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings.

[0032] 1. A H9N2 type influenza virus-like particle vaccine for prevention, its components include H9N2 type influenza virus-like particles; The envelope surface of the shell particle structure contains one or more structural proteins of the H9N2 influenza virus.

[0033] The structural protein may be hemagglutinin HA protein, neuraminidase NA protein and matrix protein M1 of H9N2 influenza virus; wherein, the amino acid sequence of the HA protein is as shown in SEQ ID NO.1, encoding the HA protein The nucleotide sequence is shown in SEQIDNO.2; the amino acid sequence of the NA protein is shown in SEQIDNO.3, and the nucleotide sequence encoding the NA protein is shown in SEQIDNO.4; the amino acid sequence of the M1 protein is shown in As shown in SEQ ID NO.5, the nucleotide sequence encoding the M1 protein is shown in SEQ ID NO.6.

[00...

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Abstract

The invention provides an H9N2 type flu virus-like particle vaccine for prevention and a preparation method thereof. The vaccine composition comprises H9N2 type flu virus-like particles, the virus-like particles are of the virus-like shell particle structure of the H9N2 type flu virus, and the shell particle structure capsule membrane surfaces contain one or more structural protein of the H9N2 type flu virus; the preparation method includes the steps that firstly, HA, NA and M1 gene DNA are synthesized, recombinant plasmid pFastBac-DuaL-HA-NA-M1 is constructed, the constructed recombinant plasmid is led into E.coLi competent state cell strain DH10Bac cells, the plasmid rBacmid-HA-NA-M1 is obtained through blue-white selection, transfection of SF9 insect cells is conducted, the virus-like particles are obtained, an adjuvant is dissolved in the virus-like particles, and the vaccine is obtained after incubation and centrifugal cleaning are obtained. By means of the H9N2 type flu virus-like particle vaccine, an organism can be stimulated to generate a strong immune response, and immunogenicity is high.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a preventive H9N2 influenza virus-like particle vaccine and a preparation method thereof. Background technique [0002] The H9N2 influenza virus, a subtype of the avian influenza virus, was first discovered in turkeys in the late 20th century. Experiments have shown that the H9N2 subtype influenza virus has strong pathogenicity to chickens, causing a decline in the immunity of chickens, causing a large number of deaths of chickens due to secondary infection, causing a decline in egg production of laying hens, and causing serious economic losses. The pathogenicity of humans is weak. On the other hand, the transmission of this virus is very strong, and it has already spread widely. The H9N2 influenza virus can not only cause the body to undergo immunosuppression, but also allow various secondary infections to occur on the host. It is reported that pigs hav...

Claims

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Application Information

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IPC IPC(8): A61K39/145A61K39/39A61P31/16C12N15/44C12N15/866C12N7/04
CPCA61K39/12A61K39/39A61K2039/5258A61K2039/55555C12N7/00C12N15/86C12N2710/14043C12N2760/16123C12N2760/16134
Inventor 蔡家利宋梅吴胜昔王远强许雁户国达张羿肖红王璐
Owner CHONGQING UNIV OF TECH
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