Method for establishing tumor multi-drug resistant cell mode and human breast cancer multi-drug resistant cell strain established by virtue of method

Abstract

The invention belongs to the field of oncobiology and relates to a method of establishing a tumor multi-drug resistant cell mode in vitro. The method comprises the step of continuously culturing a sensitive tumor cell by taking ROS as an incubation drug to enable the sensitive tumor cell to generate multi-drug resistance. Moreover, the invention further relates to a human breast cancer multi-drug resistant cell strain established by virtue of the method. The cell strain is named MCF-7 / ROS and preserved in CCTCC (China Center for Type Culture Collection), wherein the address is 430072, Wuhan University, Wuhan, China; the preservation date is 28th, January, 2015; the preservation number is CCTCC-C201520. The method provided by the invention is simple in process and stable in modeling condition, the modeling cost can be remarkably lowered, and the method has wide applicability. The MCF-7 / ROS cell strain established by the method can serve as an advantageous experimental mode for researching related mechanism, target spot exploration and screening of new drugs.

Description

technical field [0001] The invention belongs to the field of tumor biology, and in particular relates to a method for producing a mold constructing an in vitro tumor multidrug-resistant cell model by a single-machine reaction oxygen group, and also relates to a human breast cancer multidrug-resistant cell line established by the method. Background technique [0002] The construction of tumor cell models in vitro is an important experimental basis for the study of tumor occurrence, development and intervention. The choice of modeling conditions and modeling methods is the decisive factor for model construction. It is a key platform for revealing the molecular mechanism of similar tumor formation and studying tumor drug intervention. . [0003] Tumor multidrug resistance (MDR) refers to the phenomenon that tumor cells develop drug resistance after exposure to antineoplastic drugs, and also produce cross-resistance to a variety of antineoplastic drugs with different structures...

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