Bionic artificial bone and preparation method thereof

A technology of artificial bone and derivatives, which is applied in the field of medical composite materials to achieve the effects of good clinical operability, good processability and high biological safety.

A technology of artificial bone and derivatives, which is applied in the field of medical composite materials to achieve the effects of good clinical operability, good processability and high biological safety.

CN110339399AInactive Publication Date: 2019-10-18SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Bionic artificial bone and preparation method thereof
  • Bionic artificial bone and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment is a preferred bionic artificial bone (such as figure 1 Shown cylinder shape) preparation method, comprises the steps:

[0041] 1) Preparation of component A: collect a sufficient amount of diatoms with a round sieve, wash with water several times and centrifuge to remove impurities, then ultrasonically treat with 5wt% sodium chloride solution for 30min, then use 30wt% hydrogen peroxide and 2mol L -1 Treat with hydrochloric acid for 48 hours, wash with water repeatedly until neutral, then centrifuge to remove the supernatant. Sequentially wash with 50wt%, 75wt%, 100wt% ethanol ultrasonically for 30 min each, centrifuge to remove the supernatant, and vacuum dry.

[0042] 2) Preparation of component B: type I fish collagen: hyaluronic acid = 10:1 (w / w), prepared into a 4wt% solution.

[0043] 3) Composite of component A and component B: slowly add component A to component B under mild stirring condition, the ratio is A:B=1:5(w / v). Stand still for 12 hou...

Embodiment 2

[0048] This embodiment is another preferred method for preparing bionic artificial bone, comprising the following steps:

[0049] 1) Preparation of component A: collect a sufficient amount of root canal diatoms, wash with water several times, and centrifuge to remove impurities, then ultrasonically treat with 2wt% sodium chloride solution for 20 minutes, and then use 20wt% hydrogen peroxide and 3mol L -1 Treat with hydrochloric acid for 24 hours, wash with water repeatedly until neutral, then centrifuge to remove the supernatant; sequentially wash with 50wt%, 75wt%, 100wt% ethanol ultrasonically for 20min each, centrifuge to remove the supernatant, and vacuum dry;

[0050] 2) Preparation of component B: type II fish collagen polypeptide: water-soluble chitosan=12:1 (w / w), prepared into a 10wt% solution;

[0051] 3) Composite of component A and component B: slowly add component A to component B under mild stirring condition, the ratio is A:B=1:2(w / v); stand for 8 hours under ne...

Embodiment 3

[0056] This embodiment is yet another preferred method for preparing a bionic artificial bone, comprising the following steps:

[0057] 1) Preparation of component A: After collecting sufficient diatoms, wash with water several times and centrifuge to remove impurities, first treat with 3.5wt% sodium chloride solution for 15 minutes, and then use 10wt% hydrogen peroxide and 1mol L -1 Treat with hydrochloric acid for 72 hours, wash with water repeatedly until neutral, then centrifuge to remove the supernatant; sequentially wash with 50wt%, 75wt%, 100wt% ethanol ultrasonically for 15min each, centrifuge to remove the supernatant, and vacuum dry;

[0058] 2) Preparation of component B: type III porcine collagen gelatin: fucoidan = 20:1 (w / w), prepared into a 2wt% solution;

[0059] 3) Composite of component A and component B: slowly add component A to component B under mild stirring conditions, the ratio is A:B=1:1 (w / v); stand for 4 hours under negative pressure, Allow componen...

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Abstract

The invention relates to a bionic artificial bone. The artificial bone comprises a component A and a component B, wherein the component A is a marine-source biomineralized substance with a natural multiple nano structure, and the component B is an artificial extracellular matrix organic substance. Specifically, the component B in a solution form is a complex of collagen and polysaccharide, and thecomponent A mainly consists of a marine organism exoskeleton of bacillariophyta. The invention also relates to a preparation method of the bionic artificial bone. The bionic artificial bone is bionically compounded of marine-source natural inorganic materials and organic materials, has the natural multiple nano bionic structure, can guide and induce the generation of new tissue, and comprises theinorganic mineralized components of the marine-source natural multiple nano structure and the organic components of an artificial extracellular matrix; the sources are wide, the cost is low, the biological safety is high, and therefore the bionic artificial bone is adopted as a natural bionic extracellular matrix stent material. The prepared bionic artificial bone has high processability and clinical operability and can meet the clinical demands of repairing different bone defects.

Description

technical field [0001] The invention relates to the technical field of medical composite materials, in particular to a bionic artificial bone and a preparation method thereof. Background technique [0002] Bone defects can be caused by various diseases or traumas. Natural bone is composed of organic matter and inorganic salts in a ratio of about 35:65. Organic matter mainly includes collagen, polysaccharides, etc., and inorganic salts are mainly calcium salts. [0003] Many artificial bone materials have been used for bone reconstruction, which can be divided into: (1) inorganic materials: such as bioceramics, which are biocompatible but relatively inert, high hardness but brittle; (2) organic materials: such as collagen, polyester (3) Composite materials: such as calcium phosphate-collagen composite artificial bone, PLGA-BMP artificial bone, etc., have both mechanical properties and biological activity, but the osteogenic activity is still not ideal. [0004] In recent y...

Claims

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Application Information

Patent Timeline
18 Oct 2019
Publication
CN110339399A
IPC
A61L27/36; A61L27/24; A61L27/20; A61L27/50
CPC
A61L27/20; A61L27/24; A61L27/3637; A61L27/365; A61L27/50; A61L2300/412; A61L2400/12; A61L2430/02
Inventors
位晓娟; 张长青