A kind of preparation method of tissue engineering porous extracellular matrix scaffold

A tissue engineering and extracellular matrix technology is applied in the field of preparation of tissue engineering porous extracellular matrix scaffolds, which can solve problems such as limiting the application of decellularized scaffolds, and achieve the effect of broadening the application range.

Active Publication Date: 2022-03-22
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existence of these problems also limits the further application of decellularized scaffolds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Preparation of nanopore cell matrix scaffold material

[0012] Preparation of PCL nanofiber scaffold: Weigh 1.0 g of PCL with a molecular weight of 80,000, add it to 10 ml of dichloromethane, stir and dissolve at room temperature until clear, and prepare a PCL solution with a concentration fraction of 10% (m / v). Electrospinning was performed at room temperature with a relative humidity of 60%. Cover the electrospinning drum receiving device with aluminum foil and ground it, fill the 10% (m / v) PCL spinning solution into a disposable syringe with a diameter of about 14.95 mm, and connect the high voltage DC power supply to the syringe needle. Adjust the syringe needle to the center of the cylindrical receiver, set the distance between the needle and the receiver to 12 cm, the solution flow rate to 1 ml / h, the DC voltage to 16 kV, and the spinning time to 40 min. After the preparation is completed, the obtained membrane scaffold material is vacuum-dried at room...

Embodiment 2

[0017] Example 2: Preparation of cell matrix scaffold material with micron pore size

[0018] Preparation of PLA microfiber scaffold: The scaffold was prepared in a fume hood at room temperature by wet spinning. Dissolve 1 gram of polylactic acid in 10 ml of hexafluoroisopropanol, and stir at room temperature until the solution is clear. Connect a cylinder with a diameter of 3 cm to a rotating motor. The polylactic acid spinning solution was sucked into the syringe, and the needle of the syringe was placed in the ethanol coagulation bath at a position 1 cm away from the receiving cylinder. Set the flow rate of the solution to 2ml / h, the rotational speed of the receiving rod to 500rpm, and the spinning time to 40min. After the preparation is completed, the micron fiber scaffold is vacuum-dried for future use.

[0019] Subcutaneous implantation of the PLA microfiber scaffold: cut the PLA microfiber scaffold obtained above into strips of 4 cm×2 cm, sterilize with 75% medical a...

Embodiment 3

[0023] Example 3: Preparation of cell matrix scaffold material with large pore size

[0024] Preparation of PCL crude fiber scaffold: the scaffold was prepared at room temperature by melt spinning. 20 grams of PCL was added to the barrel. Raise the temperature of the barrel to 90°C for 5 hours, adjust the distance between the needle of the barrel and the receiving plate, set the advancing speed of the barrel to 6ml / h, the moving speed of the x-axis and y-axis of the receiving plate to 1mm / s, and the moving distance It is 15cm, and the spinning time is 60min. After the preparation is completed, the crude fiber scaffold is vacuum-dried for future use.

[0025] Subcutaneous implantation of the PCL crude fiber scaffold: cut the PCL crude fiber scaffold obtained above into a rectangle of 10 cm×8 cm, and sterilize it with ethylene oxide before use. After anesthetizing the sheep, the two sides of the back were shaved with a size of 12 cm × 2 cm, and an 11 cm long incision was made...

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PUM

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Abstract

The invention provides a method for preparing a porous extracellular matrix scaffold for tissue engineering. In the method, a nanometer or micrometer polymer scaffold is implanted into the subcutaneous or peritoneal cavity of a host, and the polymer scaffold is used as a template to prepare a tissue-engineered porous extracellular matrix scaffold using the immune protection mechanism of the host. Controlled pore structure, and non-immunogenic tissue engineered extracellular matrix scaffold implanted in vivo. The invention solves the problem of pore formation of extracellular matrix scaffold materials; the porous scaffold is derived from the animal itself and is used for repairing autologous damaged tissues or organs without immune rejection; at the same time, since there is no complicated decellularization step for autologous repair, the scaffold The mechanical strength of the cell matrix is ​​obviously better than that of the decellularized scaffold material; the cell matrix porous scaffold can be further decellularized for allogeneic tissue or organ repair, and has a good application prospect in the field of organ repair.

Description

technical field [0001] The invention relates to a method for preparing a porous scaffold in the field of tissue engineering, in particular to a method for preparing a porous extracellular matrix scaffold for tissue engineering. Background technique [0002] The development of tissue engineering has opened up a new path for the repair of human tissue and organ defects. At present, scientists have developed a variety of tissue engineering products and used them clinically, and achieved good results. Tissue engineering mainly includes three aspects: scaffolds, cells and signaling factors. Tissue engineering scaffold materials can be implanted in the body to match the corresponding tissues and organs, including tissue scaffold materials for bone, cartilage, nerves, blood vessels, skin and artificial organs, such as liver, kidney, spleen, urethra, etc. Scaffold materials need to meet the following conditions: good biocompatibility; controllable degradation rate; high porosity; s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/38A61L27/36A61L27/50
Inventor 朱美峰孔德领王恺王淑芳李雯
Owner NANKAI UNIV
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