30 results about "Microfilament" patented technology

The invention discloses a multi-scale artificial dermal scaffold and a preparation method thereof, and belongs to the technical field of tissue engineering and biomedical materials. The artificial dermal scaffold is of a three-dimensional porous structure formed by microfilaments of a first frame and a second frame, the microfilaments of the second frame are distributed in pores of the first frame, disordered microfilaments which do not affect the penetrability of the macropores are arranged in the macropores in the top surface of the first frame, and longitudinally aligned microfilaments are arranged in the micropores in the section of the first frame. Through the specific structure, the adhesion, proliferation and migration effects of cells can be remarkably improved, and rapid vascularization of artificial dermis is facilitated. In addition, the preparation process of the artificial dermal scaffold is simple and easy to operate, can be used for batch preparation, and has a good application prospect.

The invention discloses a gradient degradation type personalized absorbable sternum fracture fixing plate and a preparation process, and belongs to the technical field of medical implant materials. Comprising an anatomical adaptive plate body and an integrated locking assembly, the plate body is of a partitioned structure of a middle supporting area and an edge buffering area, a gradient pore array penetrating through the thickness direction is formed in the surface of the plate body, and a hydroxyapatite-collagen composite coating is loaded on the inner wall of a pore; the plate body is made of a composite absorbable material through 3D printing forming and hot pressing post-treatment, and the composite absorbable material comprises, by weight, 60%-70% of polylactic acid-glycolic acid copolymer (PLGA, LA / GA = 75 / 25), 15%-20% of silanized magnesium alloy microwires, 5%-10% of hydroxyapatite nano-particles and 5%-10% of polycaprolactone (PCL). Space-time matching of the degradation rate and the bone healing process is achieved through the partition gradient structure, the magnesium alloy microwires enhance the mechanical property and release active ions to promote osteogenesis, and the method has the advantages of personalized adaptation, gradient degradation, bone regeneration promotion and the like.

This invention provides a macroporous hydrogel with a directional structure, its preparation method, and its applications. The preparation method includes: adding a photoinitiator to a mixed solution of sodium alginate and hyaluronic acid methacrylate, stirring in the dark to form a homogeneous solution; cross-linking the homogeneous solution with a cross-linking agent to form hydrogel microfilaments, and washing away excess cross-linking agent; implanting the hydrogel microfilaments into a mold groove, and combing them in the same direction using a fine-tooth comb until a directional structure is macroscopically observed; irradiating the hydrogel with a UV lamp to promote UV cross-linking, and then removing the hydrogel microfilaments from the mold to form a macroporous SA / HA hydrogel with a directional structure. This invention utilizes a mild and simple method to prepare a macroporous hydrogel with a directional structure. This hydrogel has good biocompatibility, significantly promotes cell ingrowth and tissue integration, and promotes tendon stem cell differentiation into tendons, thus facilitating tendon repair.

The present invention relates to an absorbent composite web in which particles mixed with continuous microfilaments are arranged within the pores of a high-loft web, and to a method for forming the web, the web being applicable to filtration applications or (particularly when the particles are liquid absorbent particles) to absorbent hygiene products.

The invention provides construction and application of an intermolecular structural domain tension probe, the probe comprises a first structure, a second structure and a fluorescent protein pair eCFP and eYFP, the first structure and the second structure are respectively connected to two ends of the fluorescent protein pair, and the eCFP and the eYFP are connected through a short peptide; the first structure is a structural domain or protein capable of being combined with a cytoskeleton connecting protein, and the second structure is a structural domain or protein capable of being combined with a cytoskeleton; the cytoskeleton connecting protein is selected from talin, SYNE4, SYNE3, SYNE2, Desmoplakin and the like; the cytoskeleton is a microfilament, a microtubule or an intermediate filament. The intermolecular structural domain tension probe can be used for detecting intracellular mechanical signals, the limitation of a traditional tension probe is overcome, the adverse effect of function enhancement caused by gene cloning is avoided, a new opinion is provided for a mechanical transduction molecular mechanism, and the application has the advantages that the application range is wide, and the application prospect is wide. And a more accurate experimental tool is provided for the research of cell mechanics biology, and the effect of a mechanical signal in cell behavior regulation and control is disclosed.

The invention discloses a polysubstituted amine compound, a pharmaceutical composition containing the polysubstituted amine compound and an application of the polysubstituted amine compound in tumor resistance. The structure of the polysubstituted amine compound is shown as a formula I in the specification. Through multiple experiments, the inventor of the invention proves that the compound disclosed by the invention has a remarkable inhibiting effect on FBXO44, and can be combined with the FBXO44 in cells to inhibit down-regulation of the FBXO44 on MTSS1, so that the formation of tumor cell microfilament skeletons is disturbed, and the compound has a strong anti-proliferation effect on gastric cancer cell strains such as NUGC4 and the like. Therefore, the compound disclosed by the invention can be used as an FBXO44 inhibitor to be applied to medicines for treating solid tumors or leukemia related to human or animal cell proliferation.

The invention relates to a 3, 4, 5-trihydroxy phenylacetate compound and a preparation method and application thereof, the 3, 4, 5-trihydroxy phenylacetate compound belongs to a compound shown in a general formula I and a general formula II, R1, R2 and corresponding derivatives thereof can activate a copper death pathway mediated by endogenous copper ions, have an obvious anti-tumor effect, and have a stronger anti-tumor effect in chemotherapy of drug-resistant tumors. The tumors comprise melanoma, liver cancer, gastric cancer, breast cancer, oral squamous cell carcinoma and the like. The compound is a non-copper ion carrier compound with a brand new structure, induced copper death does not depend on exogenous copper ions, and endogenous copper ions are dissociated by reducing the content of glutathione which is a copper ion chelate in cells. The copper ions are further combined with microfilament skeleton related protein to cause disintegration of the microfilament skeleton, so that copper death is induced. The compound disclosed by the invention can be used as a potential lead compound for inducing endogenous copper ion mediated copper death for anti-tumor therapy.

The application discloses a microtube holder convenient for operation under a microscope and relates to the technical field of holders. The microtube holder is not only exquisite and flexible but also does not shield a microtube or a microfilament under the premise that the microtube or the microfilament can be reliably clamped, so that all movement tracks of the lower end of the microtube or the microfilament on an operation interface can be seen under the microscope, and the real-time position of the lower end of the microtube or the microfilament can be tracked. The microtube holder comprises a first clamping piece, a second clamping piece and a plurality of limiting pins, the first clamping piece and the second clamping piece are oppositely arranged, the front end of the first clamping piece is provided with a first clamping part, the rear end of the first clamping piece is provided with a first connecting part, and the top of the first clamping piece is provided with a connecting rod, the front end of the second clamping piece is provided with a second clamping part, the rear end of the second clamping piece is provided with a second connecting part, the first connecting part and the second connecting part are both slidably connected to the limiting pins, a first permanent magnet is arranged in the first connecting part, a second permanent magnet is arranged in the second connecting part, and the first permanent magnet and the second permanent magnet are attracted to each other. The application is used for improving the performance of the microtube holder.

This application discloses a microfilament electrode structure, its fabrication method, and its implantation method. The microfilament electrode structure includes: a substrate having a microfilament region and a bonding region; multiple electrode filament layers, each layer comprising multiple microfilament structures located on the microfilament region and a limiting structure, each microfilament structure having an electrode site on one side surface; any adjacent electrode filament layers being connected in the bonding region and relatively independent in the microfilament region; the limiting structure being fixed to one side surface of the microfilament structure; and bonding pads located in the bonding region and electrically connected to the electrode sites one-to-one. This microfilament electrode structure solves the problems of folding and breakage risks and low wafer area utilization in planar microfilament electrode structures of related technologies during implantation.

A method for manufacturing a microfiber implant. The method includes a winding platform and The method uses an apparatus consisting of a feeder head, which is connected to a winding plug. Advance the microfilament towards the take-up platform and wrap it around the The feeder head is then rotated to the position where the microfilament is being wound. The feeder head moves laterally relative to the winding platform. Multiple sweeps can be performed to build up layers of coating, resulting in microfabrication. A fiber patch is obtained, which is then further processed (e.g., by applying a collagen coating). This winding technology creates a microfiber implant. Microfiber implants and devices for winding are also disclosed.

The utility model relates to the technical field of measuring instruments, in particular to a micro-filament stiffness measuring device. The micro-filament stiffness measuring device comprises a testing platform, a positioning piece and a measuring assembly. The test platform is horizontally arranged and is used for providing support for a to-be-tested fine wire, and one end of the fine wire extends out of the test platform and naturally droops. And the positioning piece is used for positioning the part of the micro-filament on the test platform so as to ensure that the micro-filament does not tilt. And the measuring assembly is used for measuring the length and the sagging height of the micro-filament extending out of the testing platform, so that the stiffness of the micro-filament can be calculated according to a measuring structure and a formula. In conclusion, the device for measuring the stiffness of the microfilaments can be used for evaluating the stiffness performance of the microfilaments made of various materials, so that the stiffness of the microfilaments can adopt a unified measurement standard and process, and the reliability and consistency of measurement results can be improved.

The application provides a blood-derived mesenchymal stem cell ovary targeting complex and a preparation method thereof. The ternary gel matrix system of the application is composed of sodium hyaluronate, four-arm polyethylene glycol maleimide and mercapto carboxymethyl dextran in the second precursor solution, a thioether bond crosslinking network is formed in situ through a maleimide-mercapto Michael addition reaction, and ovarian decellularized extracellular matrix microfilaments are introduced as a biomimetic support unit, and the blood-derived mesenchymal stem cells are encapsulated into injectable microtissue unit preparations with an arithmetic mean of 150-800 mu m and a particle size variation coefficient of not more than 15% through a microfluidic device. The application realizes the synergistic optimization of the injectable fluidity of the gel matrix and the rapid forming stability in the body, significantly improves the cell viability protection effect and the microtissue unit batch particle size uniformity, and solves the technical contradiction that the injectability and structural stability cannot be considered in the existing injectable cell preparations.

The application provides an implantable bioelectrode with a structure simulating a Cuscuta suction organ and a preparation method thereof, and relates to the technical field of medical devices. The implantable bioelectrode comprises an electrode substrate and a biomimetic interface coating arranged on the surface of the electrode substrate; the biomimetic interface coating comprises, from inside to outside, a conductive buffer layer, an imitated invasive microfilament layer and a tissue adhesion layer. The implantable bioelectrode utilizes the synergistic effect of the three-layer biomimetic interface to improve the connection stability. The outer adhesion film provides rapid and non-invasive in-situ fixation, the middle nanowire array realizes minimally invasive probe-type anchoring, and the inner hydrogel provides mechanical buffering and eliminates modulus mismatch. The structure effectively overcomes the interface wear and signal attenuation caused by physiological micro-motion, and realizes long-term, stable and low-impedance adhesion.

The invention provides a method for marking a microfilament skeleton in a living cell by using ligand-modified magnetic nanoparticles and application of the microfilament skeleton. The method comprises the following steps: preparing ligand modified magnetic nanoparticles; culturing living cells to be marked to obtain adherent cells; incubating the adherent cells and the ligand modified magnetic nanoparticles to obtain marked cells; and observing the marked intracellular microfilament skeleton by using a laser confocal imaging system. The ligand-modified magnetic nanoparticles prepared by the method have good biocompatibility, magnetic responsiveness and microfilament targeted binding characteristics, microfilament skeletons in living cells are labeled by the ligand-modified magnetic nanoparticles, and then distribution of the microfilament skeletons in the living cells can be clearly observed by using a laser confocal imaging system. According to the method, the microfilament skeleton in the living cell can be marked and clearly imaged, the distribution of the microfilament skeleton in the living cell can be deeply observed, and the method has a good application prospect in the fields of biomedical treatment, cell function research and the like.

The invention discloses a near-infrared self-flickering fluorescent dye as well as a preparation method and application thereof. The near-infrared self-flickering fluorescent dye has one of structures as shown in a formula I and a formula II. Azetidine is introduced to inhibit a twisted charge transfer effect in molecules, so that silicon rhodamine has the characteristics of high light stability, high brightness, cell permeability and the like. Based on thermodynamic equilibrium of intramolecular spiralization of a benzyl alcohol structure in the dye, the fluorescent dye can realize single-molecule localization super-resolution fluorescence imaging of various subcellular structures in living cells, and realizes visualization of structures including lysosomes, mitochondria, cell nucleuses, microfilaments and the like on a nano scale. Besides, the absorption wavelength of the dye is 650nm, the fluorescence emission wavelength reaches 666nm and reaches a near-infrared region, damage of exciting light to cells is reduced in the super-resolution imaging process, and the dynamic life process of the cells under normal physiological conditions can be visualized;

The present invention relates to an absorbent composite web in which particles mixed with continuous microfilaments are located within the pores of a highly bulky web, as well as to a method of forming such a web that can be applied for filtration, or in absorbent hygiene articles, particularly if the particles are liquid absorbent particles.

The invention relates to the technical field of cell targeted therapy, in particular to a construction method and application of a cell robot for enhancing cell therapy targeting and entity penetration. The invention relates to a construction method of a cell robot for enhancing cell therapy targeting and entity penetration. The construction method comprises the following steps: coupling magnetic microfilaments on the surfaces of cells; the magnetic microfilament comprises a microfilament and magnetic particles attached to the microfilament; and the microfilaments are made of polylactic acid-glycolic acid copolymer. According to the technical scheme, the technical problem that in the prior art, chimeric antigen receptor modified macrophages with high targeting and penetrating capacity are lacked can be solved, and the technical problems that in the prior art, chimeric antigen receptor cells generally face infiltration of solid tissues and infiltration is not ideal are further solved. The engineering cell design combining the bionic sperm model and magnetic driving breaks through the bottleneck of the traditional cell therapy in solid tissue delivery, provides a brand new technical thought and method for wider cell therapy optimization, and has ideal application and popularization values.

The present invention relates to textiles comprising non-regenerated microfibrillated cellulose in the form of fibrous microfilaments. Additionally, the present invention relates to methods for preparing and using such textiles and / or monofilaments.

The invention provides an OFD1 gene capable of promoting occurrence and development of tumors as well as an expression product and application of the OFD1 gene. The OFD1-regulated central body actin skeleton network branch inspection mechanism is found and verified for the first time, targeting of the inspection mechanism or regulation of a local actin skeleton network with OFD1 has the remarkable advantage of low toxicity, and a new target and thought are provided for diagnosis and inhibition of malignant tumors. It is successfully identified that a conservative hydrophobic acidic VCA structural domain exists at the C tail end of the OFD1 protein, is used for being combined with an Arp2 / 3 compound and cooperates with I-type NFPs to promote branching of a centrosome actin skeleton network. A polypeptide is designed aiming at the VCA structural domain, the regulation effect of the polypeptide on a microfilament skeleton is verified, and regulation of different fate of normal cells and tumor cells is realized.

The application provides a cytoskeleton modeling method based on dissipative particle dynamics simulation, comprising the following steps: step 1, setting a cell region in a dissipative particle dynamics simulation software; step 2, randomly distributing microfilament single chains which do not intersect each other in the cell region; step 3, randomly generating a plurality of free cross-linking protein particles in the cell region; step 4, the microfilament single chains and the cross-linking protein particles are polymerized / dissociated until the number of the cross-linking protein particles in three states tends to be stable, the three states of the cross-linking protein particles are free, combined with only one microfilament single chain and combined with two microfilament single chains; step 5, deleting the cross-linking protein particles and directly connecting two microfilament single chains connected by the same cross-linking protein particle; and step 6, calculating cytoskeleton parameters and outputting a cytoskeleton model. The cytoskeleton constructed by the application has high fidelity and presents mechanical characteristics such as viscoelasticity under the action of an external force.

The application discloses a cytoskeleton parameter testing method based on a random forest algorithm, and cells are cultured by using a modified culture dish; when the cells in the dish continuously proliferate and cover 60% of the bottom area of the culture dish, the positions of selected experimental cells are marked by using an AFM, and mechanical parameters and topographic parameters of the experimental cells are obtained; meanwhile, Tubulin-tackerRed and Action-TrackerGreen are used to dye the cytoskeleton of the selected experimental cells, and the microfilament fluorescence intensity and the microtubule fluorescence intensity of the cells are obtained, which are used as cytoskeleton parameters. The cytoskeleton parameters are regressed and predicted according to the mechanical parameters and the topographic parameters of the selected cells by using the random forest algorithm. The cytoskeleton parameters of the cells can be quickly obtained by the application, and the economic and time costs are saved; and the cells are non-toxic and non-injurious during the detection of the topographic parameters and the mechanical parameters by using the AFM. After the detection is completed, the cells still have activity and can be used for other detections.

This application discloses a pre-assembled structure, pre-assembled method, and implantation method for a microfilament electrode. The pre-assembled structure includes: a microfilament electrode having a substrate and multiple microfilament structures attached to the substrate; an organic thin film with the microfilament structures arranged on it; and a rigid implantation tool fixedly connected to the microfilament structures. The rigid implantation tool is used to implant the electrode recording sites on the microfilament structures into a target object. The technical solution of this application effectively solves the problems in the prior art where microfilament electrodes easily adhere to the substrate during drying, leading to separation difficulties, and where dried microfilament electrodes are unstable in air, easily drifting and entangled, causing operational difficulties in subsequent implantation steps.

The application relates to the technical field of micro-filament drawing, in particular to a dynamic adjustment method and device for high-speed drawing force of micro-filaments, which are applied to a dynamic adjustment system for high-speed drawing force of micro-filaments, based on a pressure sensor, real-time measurement of drawing resistance generated by a drawing unit in the process of reducing the diameter of a wire, and based on a force sensor, real-time measurement of counter-tension of the wire in the transmission process; dynamic adjustment of the current measured counter-tension is carried out according to an established counter-tension regulation strategy; wherein the counter-tension regulation strategy comprises a first relational expression for limiting counter-tension utilization rate, and a second relational expression for representing the mapping relationship between the drawing force and the breaking force of the wire after the diameter reduction by the drawing unit. Thus, in the high-speed drawing process, the drawing force of the wire is always maintained in a stable state, effectively reducing the problems of wire breakage, surface quality defects and die wear, improving the production efficiency and the finished product quality of the micro-filaments, and prolonging the service life of the die.

The present application relates to the use of a plant fermentation product, in particular the use of a Imperata cylindra fermentation product for increasing the expression of genes, promoting the proliferation of collagen and elastin, and increasing the antioxidant capacity. The present application provides an Imperata cylindra fermentation product prepared by two-stage fermentation of an Imperata cylindra water extract with yeast and lactobacillus. The present application also provides the use of an Imperata cylindra fermentation product for preparing a composition for increasing the expression of keratin gene, polykeratin microfilament gene and hyaluronic acid synthase gene, promoting the proliferation of collagen and elastin, and increasing the antioxidant capacity. The Imperata cylindra fermentation product can effectively increase the skin moisturizing capacity, maintain the arrangement of keratinocytes, maintain the integrity of the stratum corneum structure, improve the skin barrier function, make the skin firm and elastic, and prevent skin aging.

The invention provides a microfilament binding protein sequence design method and device. The method comprises the following steps: randomly generating an initial amino acid sequence; carrying out random mutation on single amino acid by starting from the initial amino acid sequence to obtain a mutated amino acid sequence, and scoring the amino acid sequences before and after mutation according to a scoring function; and selecting the amino acid sequence of which the score meets a preset condition to carry out next round of random mutation until a preset number of iterations is reached, thereby obtaining a design sequence. According to the method, the microfilament binding protein sequence is accurately designed under the small sample condition.

The invention discloses a high-precision grinding and polishing clamp for microfilament sample column end face positioning and modular quick change, and belongs to the technical field of electron microscope sample preparation. The invention discloses a high-precision grinding and polishing clamp for microfilament sample column end face positioning and modular quick change. The high-precision grinding and polishing clamp comprises a clamping base, a microfilament positioning module and an end face gland. According to the invention, the problems of poor clamping stability, low positioning precision, easy damage of samples, low operation efficiency and incapability of batch processing of microwires with the diameter of 10-100 [mu] m in the existing clamping technology are solved, and controllable vertical pre-tightening force is applied by the end face gland to realize high-precision coplanar positioning of the end faces of the microwires; flexible lossless constraint on the side face is achieved through a stepped micropore array with a micro-texture inner wall or a V-shaped groove array with a flexible liner, and efficient, lossless, batched and repeatable high-quality preparation of microfilament samples is achieved through modular quick-change design and a high-precision repeated positioning interface.

The utility model relates to the technical field of medical instruments, in particular to a medical long tube type device connecting mechanism which comprises an inner shell used for clamping a plurality of bundles of microwires, an outer shell is clamped in the inner shell, a connecting mechanism body is arranged on one side of the outer shell, and the connecting mechanism body comprises a filling channel tube clamped on the inner wall of the inner shell. And a limiting hook clamped with the inner wall of the shell is arranged at one end of the filling channel pipe, the filling channel pipe is sleeved with a separation pipe, and the separation pipe is connected with the shell in an abutting mode. After the multiple beams of microfilaments and the connecting mechanism are installed and inserted into the simulation cavity, the multiple beams of microfilaments are attached to the inner cavity, tissue enters the multiple beams of microfilaments through the filling channel pipe till the interiors of the multiple beams of microfilaments are filled, the separating pipe is fixed, the filling channel pipe is pulled out forcibly, and the multiple beams of microfilaments are integrally separated from the connecting mechanism and are convenient to disassemble. The filling speed and the inner diameter of the tissue are guaranteed to a great extent, and the damage degree is greatly reduced.

This invention relates to an apparatus for preparing liquid metal microfilaments, comprising a water tank, inside which are fixed tweezers and movable tweezers. The movable tweezers are driven by an electric mechanism to reciprocate along the stretching direction of the liquid metal microfilaments. A temperature control component is provided on the water tank. This invention has a simple structure, good processability, and can efficiently prepare liquid metal microfilaments with a diameter of less than 100 μm that have good stability and high repeatability.

The invention relates to the field of sewage treatment, in particular to a treatment method for secondary sedimentation tank float sludge initiated by a DCM control agent in sulfate pulping. The method comprises a step of adding a fusion protein as shown in SEQ ID NO.3 into a secondary sedimentation tank, and further comprises the following steps: regulating the concentration of dissolved oxygen to 2.0-4.0 mg / L, the temperature to 35-40 DEG C and the pH value to 6.8-7.2, hydrolyzing extracellular proteins or extracellular polysaccharides generated by various zoogloea bacteria in the secondary sedimentation tank, destroying a foam skeleton and reducing adhesion of microhyphae, thereby realizing the purpose of removing the microhyphae in the secondary sedimentation tank. And the problem of'combined action of a DCM control agent and filamentous bacteria 'is solved, and the problem of float sludge in a secondary sedimentation tank is solved.