Tissue engineering bone/cartilage double-layer scaffold and construction method and application thereof

A technology for tissue engineering bone and cartilage, applied in bone implants, medical science, prostheses, etc., can solve problems such as inability to load in the body, poor gel mechanical properties, poor bone-cartilage interface integration, etc., to achieve Combining the effects of good, broad source, good biocompatibility and degradability

Inactive Publication Date: 2009-11-11
GENERAL HOSPITAL OF PLA
View PDF0 Cites 34 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Assembly methods include fibrin gel bonding, atraumatic suture suture method, or polylactic acid stick bonding fixation, etc., but the bone-cartilage complex constructed has the problem of poor bone-cartilage interface integration (Schaefer D, Martin I, Shastri P, et al. In vitro generation of osteochondralcomposites. Biomaterials JT. Biomaterials, 2000, 21: 2599-606)
[0003] Recently foreign countries have used cancellous bone and agarose gel wrapped in chondrocytes to culture ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue engineering bone/cartilage double-layer scaffold and construction method and application thereof
  • Tissue engineering bone/cartilage double-layer scaffold and construction method and application thereof
  • Tissue engineering bone/cartilage double-layer scaffold and construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Prepare a suspension of cartilage decellularized microfilaments at a concentration of 0.5% to 5%

[0049] Human articular cartilage (provided by the Bone Tissue Bank of PLA General Hospital) was rinsed with normal saline, and soaked in 50mmol / L Tris-HCL buffer (pH7.5), which contained protease inhibitor PMSF (0.035mmol / L L), crushed by a pulverizer, adopt the density gradient centrifugation method to get cartilage filaments of about 100nm-5 μm, add 1% TritonX-100 Tris-HCL buffer solution (pH7.5) at 4°C, the buffer solution also contains Protease inhibitor PMSF (0.035mmol / L), continuously shake at room temperature for 48 hours, digest with DNaseI and RNase A at 37°C for 2-3 hours, and keep stirring, wash repeatedly with triple distilled water after centrifugation, and make 0.5% ~5% (weight ratio) emulsion suspension;

[0050] 2. Preparation of decellularized bone scaffolds

[0051] Using fresh human cancellous bone (provided by the Bone Tissue Bank of PLA General Ho...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a tissue engineering bone/cartilage double-layer scaffold and a construction method and application in medical science. The method adopts natural cartilage and bone as materials, respectively removes the antigenicity of the cartilage and the bone through de-cell, and adopts a freezing and drying method to prepare the double-layer scaffold simultaneously suitable for growthof the two issues of the bone and the cartilage. A preparation method comprises that: acellular bone is soaked in suspension of cartilage acellular matrix microfilaments, and the double-layer scaffoldof which the upper layer is a cartilage acellular matrix three-dimensional porous spongy scaffold and the lower layer is an acellular bone scaffold is prepared by the freezing and drying method and acrosslinking method. The cartilage acellular matrix spongy scaffold part of the double-layer scaffold can be introduced with factors promoting the formation of the cartilage, and the acellular bone part can be introduced with factors promoting the formation of the bone, and the factors can be respectively inoculated with cells for forming the cartilage or the bone to construct a living tissue engineering bone/cartilage complex, which is used for repairing bone and cartilage complex defect or total joint defect clinically.

Description

technical field [0001] The present invention relates to a double-layer scaffold suitable for the growth of bone and cartilage, in particular to a histocompatible double-layer scaffold for guiding bone and cartilage composite tissue regeneration and its construction method. The present invention also relates to the double-layer scaffold The use of the bracket in medicine belongs to the field of tissue engineering. Background technique [0002] Tissue engineering is an interdisciplinary subject involving cell biology, material science, engineering and bioreactors. It uses the basic principles and methods of life science and engineering to construct the tissues needed by the human body for repairing and replacing trauma, Diseased non-functioning tissue (organ). The main content of tissue engineering research includes: seed cells, scaffold materials, growth factors. Seed cells include chondrocytes, bone marrow stromal stem cells, adipose-derived stem cells, and genetically eng...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/40A61L27/38A61F2/28
Inventor 卢世璧杨强彭江黄靖香孙明学张莉眭翔赵斌郭全义汪爱媛许文静田玥姚军
Owner GENERAL HOSPITAL OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap