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Osteochondral integrated repairing gradient hybrid support material and preparation method thereof

A scaffold material and bone cartilage technology, applied in prosthetics, additive processing, medical science, etc., can solve problems such as lack of mechanical functions, poor integration of host interface, etc., and achieve good repeatability, standardization, and simple operation.

Inactive Publication Date: 2018-09-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the shortcomings and deficiencies of the existing tissue engineering osteochondral scaffolds, that is, poor integration with the host interface and lack of corresponding mechanical functions, the purpose of the present invention is to provide a cartilage-integrated gradient hybrid scaffold for internal and external repair. The structure and mechanical strength meet the requirements of osteochondral repair, and the functional requirements required for each layer of the scaffold are realized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for preparing a gradient hybrid scaffold material for osteochondral integrated repair, comprising the following steps:

[0047] 1. Using computer-aided design software to establish a CAD model: a cylindrical scaffold with a diameter of 8 mm and a height of 10 mm. The scaffold consists of three parts from top to bottom: cartilage layer, cartilage calcification layer and subchondral bone layer. The thickness was 5 mm, the thickness of the calcified cartilage layer was 100 μm, the thickness of the subchondral bone layer was 5 mm, and the pore diameter of the calcified cartilage layer was 0 μm; the pore diameter of both the cartilage layer and the subchondral bone layer was 400 μm.

[0048] 2. Convert the designed CAD model into a rapid prototyping file format (STL) and import it into the 3D-Bioplotter™ printing device. Use the multi-nozzle printing system to print out the three-dimensional porous osteochondral integrated scaffold according to the preset parameters....

Embodiment 2

[0061] A method for preparing a gradient hybrid scaffold material for osteochondral integrated repair, which is basically the same as in Example 1, except that:

[0062] Established CAD model: the thickness of the cartilage layer is 2.5mm, and the thickness of the subchondral bone layer is 7.5mm;

[0063] The preparation of the cartilage layer hybrid gel is the same as in Example 1.

[0064] The preparation of the hybrid gel of the calcified cartilage layer and the subchondral bone layer is the same as in Example 1.

[0065] The set printing parameters are as follows: the internal hole structure of the bracket is rhombus.

[0066] Mechanical tests showed that the compressive strength of the integrated stent was 0.28±0.14 MPa, and the Young's modulus was 0.44±0.27 MPa.

[0067] The porosity test shows that the porosity of the integrated scaffold is (41.32±0.46)%.

Embodiment 3

[0069] A method for preparing a gradient hybrid scaffold material for osteochondral integrated repair, which is basically the same as in Example 1, except that:

[0070] The established CAD model: the thickness of the cartilage layer is 2.5 mm, the thickness of the calcification layer is 150 μm, and the thickness of the subchondral bone layer is 7.5 mm.

[0071] The preparation of the hybrid gel of the cartilage layer and cartilage calcification layer is the same as in Example 1.

[0072] In the preparation of the subchondral bone layer hybrid gel: weigh Ca(NO 3 ) 2 . 4H 2 O, H 3 PO 4 (Guarantee the molar ratio of Ca / P is 1.67) sequentially add to liquid A, stir well until mixed evenly to obtain liquid C; put the obtained milky white sol into a 3D printing barrel, and place it at a temperature of 40°C , let it stand in a constant temperature and humidity box with a humidity of 50% for 56 hours to make it fully gel;

[0073] The set printing parameters are: extrusion air...

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Abstract

The invention discloses an osteochondral integrated repairing gradient hybrid support material and a preparation method thereof. The hybrid support material successively consists of a cartilage layer,a cartilage calcification layer and a calcification lower bone layer from top to bottom, wherein all layers are closely combined into a whole body by virtue of a 3D printing technology. The preparation method comprises the following steps: (1) separately preparing hybrid gel of each layer; (2) establishing an osteochondral integrated support model, and preparing a pre-gel support by virtue of a 3D printing technology; and (3) preparing the osteochondral integrated repairing gradient support by adopting a freeze drying technology. The integrated support prepared by the invention simultaneouslyhas a material gradient and a structure gradient, the cartilage layer, and the bone layer are closely connected into a whole body by virtue of the cartilage calcification layer, so that an effect forisolating the cell migration between the two layers can be realized; and by virtue of the 3D printing technology, the individualized tissue engineering support can be rapidly and precisely prepared according to the requirements of different patients, and the integrated construction of a complicated appearance and internal micro-structure of the support can be realized, and the application potential is great.

Description

technical field [0001] The invention belongs to the technical field of preparation of medical biological materials, and relates to a three-dimensional osteochondral scaffold and a preparation method thereof, in particular to an osteochondral integrated repair gradient hybrid scaffold material and a preparation method thereof. Background technique [0002] Osteochondral defects of joints caused by trauma, sports injuries, various arthritis diseases and other factors are very common in clinical practice, mainly manifested as intractable pain and joint movement disorders, which seriously affect the quality of life of patients, and have become the main cause of physical disabilities. One of the main reasons. At present, it is difficult to treat the osteochondral defect of this joint, and satisfactory results have not yet been achieved. Conservative treatment and joint cleaning surgery can only temporarily relieve the pain, but cannot cure it; It is the gold standard, but there ...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/56A61L27/26A61L27/12A61L27/02A61L27/50B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/025A61L27/12A61L27/26A61L27/50A61L27/56A61L2430/02A61L2430/06B33Y10/00B33Y70/00B33Y80/00C08L89/00C08L5/08
Inventor 赵娜如董怡帆
Owner SOUTH CHINA UNIV OF TECH
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