A 3D biomimetic biological scaffold containing stem cell exosomes and its application

Abstract

The invention provides a 3D biomimetic biological scaffold containing stem cell exosomes and its application. The biomimetic scaffold uses methacrylic anhydride gelatin, oxidized hyaluronic acid and dopamine-modified hyaluronic acid as the main material, and adds cartilage decellularization Matrix (DCM) or bone decellularized matrix (DBM) mimics the microenvironment of cartilage or osteochondral bone and adds mesenchymal stem cell exosomes (Exos) as a bioink, using 3D printing bioinks to construct containing Hydrogel‑DCM‑Exos and Hydrogel‑DBM‑Exos, a composite scaffold that mimics the bioactivity of cartilage and subchondral bone while promoting healing of articular cartilage and subarticular bone.

Description

technical field [0001] The invention belongs to the field of medical devices, and in particular relates to a 3D bionic biological scaffold containing stem cell exosomes and its application. Background technique [0002] Articular cartilage is one of the important components of the locomotion system. It is connected to the surface of the bone end in the joint and has a smooth and elastic texture. Articular cartilage has the functions of lubricating joints, cushioning the vibration and impact generated by movement, improving the movement and shape matching between bones, and is very important for maintaining the normal movement function of joints. Cartilage defects can cause severe pain, joint swelling, decreased mobility, and degeneration of surrounding cartilage and subchondral bone, and even develop into osteoarthritis, seriously affecting the quality of life of patients and bringing heavy economic burdens to individuals and society. With the increasing enthusiasm of the C...

AI Technical Summary

Problems solved by technology

Although considerable progress has been made in the research and development of synthetic polymer materials, metal and ceramic biomaterials and their application in cartilage or bone tissue engineering in recent years, there are obvious limitations in inducing the regeneration of articular cartilage and subchondral bone tissue at the same time, including Retention of acidic metabolites, slow degradation rate, incomplete tissue replacement, etc. are not conducive to the establishment of cartilage and subchondral bone microenvironment at the same time, making it difficult to safely and efficiently promote good repair of different tissue defects in situ

[0005] It has been confirmed that the experimental group that directly injects exosomes into the tissue to be repaired has a better repair effect than the control group, but the risk of infection caused by multiple injections and the diffusion of exosome suspension in the joint cavity reduce its therapeutic effect. efficiency

[0006] The traditional freeze-drying method is difficult to prepare different space-specific biomimetic scaffolds, and the emergence of 3D bioprinting technology provides a new method for accurately constructing biomimetic structures to simulate and reshape the three-dimensional spatial structure of different tissues

However, due to the low mechanical strength of simple acellular matrix materials, the "collapse effect" that occurs after printing multiple layers makes it impossible to maintain the spatial arrangement of cartilage and subchondral bone

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