3D bionic biological scaffold containing stem cell exosomes and application

A technology of exosomes and bio-inks, applied in 3D bionic bio-stents and applications, can solve the problems of incomplete tissue replacement, slow degradation rate, retention of acidic metabolites, etc., and achieve good slow-release repair effect and long-term slow-release time , the effect of promoting proliferation

Active Publication Date: 2021-09-17
PEKING UNIV THIRD HOSPITAL
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  • Abstract
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Problems solved by technology

Although considerable progress has been made in the research and development of synthetic polymer materials, metal and ceramic biomaterials and their application in cartilage or bone tissue engineering in recent years, there are obvious limitations in inducing the regeneration of articular cartilage and subchondral bone tissue at the same time, including Retention of acidic metabolites, slow degradation rate, incomplete tissue replacement, etc. are not conducive to the establishment of cartilage and subchondral bone microenvironment at the same time, making it difficult to safely and efficiently promote good repair of different tissue defects in situ
[0005] It has been confirmed that the experimental group that directly injects exosomes into the tissue to be r

Method used

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  • 3D bionic biological scaffold containing stem cell exosomes and application
  • 3D bionic biological scaffold containing stem cell exosomes and application
  • 3D bionic biological scaffold containing stem cell exosomes and application

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Embodiment Construction

[0042] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, but not to limit the present invention.

[0043] 1. Experimental method

[0044] 1.1 Chondrogenic differentiation

[0045] (1) When the second-generation adipose-derived mesenchymal stem cells (ADSCs) grow to 90% density, add 2 mL of 0.25% trypsin digestion solution, and observe the cell morphology under an inverted microscope for 1–2 minutes (min). Digestion was terminated by the addition of an equal volume of complete medium when cells were found to have retracted, rounded, gaps enlarged and suspended.

[0046] (2) Transfer the cell suspension to a centrifuge tube, centrifuge at 800 rpm for 5 min.

[0047] (3) Count the cell suspension using an automatic cell counter and adjust the cell density to 1.0 × 10 7 Cells / ...

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Abstract

The invention provides a 3D bionic biological scaffold containing stem cell exosomes and application. The bionic scaffold is characterized in that methacrylic anhydride gelatin, oxidized hyaluronic acid and dopamine modified hyaluronic acid are used as main body materials; a cartilage acellular matrix (DCM) or a bone acellular matrix (DBM) is added to simulate a cartilage or subchondral bone microenvironment; mesenchymal stem cell exosomes (Exos) are added and mixed to be used as biological ink; and 3D printing biological ink is used for building a composite scaffold containing Hydrogel-DCM-Exos and Hydrogel-DBM-Exos. The composite scaffold simulates the biological activity of the cartilage and the subchondral bone; and meanwhile, the healing of the articular cartilage and the subchondral bone is promoted.

Description

technical field [0001] The invention belongs to the field of medical devices, in particular to a 3D bionic biological scaffold containing stem cell exosomes and its application. Background technique [0002] Articular cartilage is one of the important components of the motor system. It is connected with the surface of the intra-articular bone end and has a smooth and elastic texture. Articular cartilage lubricates joints, cushions vibrations and shocks from movement, and improves bone-to-bone movement and morphological matching. It is essential for maintaining normal joint function. Cartilage defects can cause severe pain, joint swelling, decreased mobility, and degeneration of surrounding cartilage and subchondral bone, and even develop into osteoarthritis, which seriously affects the quality of life of patients and brings a heavy economic burden to individuals and society. With the increasing enthusiasm of the Chinese people for physical exercise, the types of sports acti...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/20A61L27/22A61L27/54B33Y70/10
CPCA61L27/222A61L27/20A61L27/3612A61L27/3608A61L27/3604A61L27/3654A61L27/54B33Y70/10A61L2300/30A61L2300/412A61L2300/602A61L2430/06A61L2430/24C08L89/00C08L5/08
Inventor 敖英芳李琪胡晓青
Owner PEKING UNIV THIRD HOSPITAL
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