3D bionic biological scaffold containing stem cell exosomes and application

Abstract

The invention provides a 3D bionic biological scaffold containing stem cell exosomes and application. The bionic scaffold is characterized in that methacrylic anhydride gelatin, oxidized hyaluronic acid and dopamine modified hyaluronic acid are used as main body materials; a cartilage acellular matrix (DCM) or a bone acellular matrix (DBM) is added to simulate a cartilage or subchondral bone microenvironment; mesenchymal stem cell exosomes (Exos) are added and mixed to be used as biological ink; and 3D printing biological ink is used for building a composite scaffold containing Hydrogel-DCM-Exos and Hydrogel-DBM-Exos. The composite scaffold simulates the biological activity of the cartilage and the subchondral bone; and meanwhile, the healing of the articular cartilage and the subchondral bone is promoted.

Description

technical field [0001] The invention belongs to the field of medical devices, in particular to a 3D bionic biological scaffold containing stem cell exosomes and its application. Background technique [0002] Articular cartilage is one of the important components of the motor system. It is connected with the surface of the intra-articular bone end and has a smooth and elastic texture. Articular cartilage lubricates joints, cushions vibrations and shocks from movement, and improves bone-to-bone movement and morphological matching. It is essential for maintaining normal joint function. Cartilage defects can cause severe pain, joint swelling, decreased mobility, and degeneration of surrounding cartilage and subchondral bone, and even develop into osteoarthritis, which seriously affects the quality of life of patients and brings a heavy economic burden to individuals and society. With the increasing enthusiasm of the Chinese people for physical exercise, the types of sports acti...

AI Technical Summary

Problems solved by technology

Although considerable progress has been made in the research and development of synthetic polymer materials, metal and ceramic biomaterials and their application in cartilage or bone tissue engineering in recent years, there are obvious limitations in inducing the regeneration of articular cartilage and subchondral bone tissue at the same time, including Retention of acidic metabolites, slow degradation rate, incomplete tissue replacement, etc. are not conducive to the establishment of cartilage and subchondral bone microenvironment at the same time, making it difficult to safely and efficiently promote good repair of different tissue defects in situ

[0005] It has been confirmed that the experimental group that directly injects exosomes into the tissue to be repaired has a better repair effect than the control group, but the risk of infection caused by multiple injections and the diffusion of exosome suspension in the joint cavity reduce its therapeutic effect. efficiency

[0006] The traditional freeze-drying method is difficult to prepare different space-specific biomimetic scaffolds, and the emergence of 3D bioprinting technology provides a new method for accurately constructing biomimetic structures to simulate and reshape the three-dimensional spatial structure of different tissues

However, due to the low mechanical strength of simple acellular matrix materials, the "collapse effect" that occurs after printing multiple layers makes it impossible to maintain the spatial arrangement of cartilage and subchondral bone

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