A kind of bioink containing stem cell exosome and its manufacturing method

A bio-ink and exosome technology, applied in the field of medical devices, can solve the problems of lack of bionic properties, low mechanical strength, collapse effect, etc., and achieve the effect of good cartilage formation, high efficiency, and promotion of proliferation.

Active Publication Date: 2022-01-07
PEKING UNIV THIRD HOSPITAL
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Problems solved by technology

However, due to the low mechanical strength of simple acellular matrix materials, the "collapse effect" that is prone to occur after printing multiple layers makes it impossible to maintain the spatial arrangement of bionic scaffolds.
Although other 3D printed biomaterials can promote the differentiation of chondrocytes and repair cartilage damage, the final repair effect is not good, and they do not have bionic performance or poor bionic performance.

Method used

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  • A kind of bioink containing stem cell exosome and its manufacturing method
  • A kind of bioink containing stem cell exosome and its manufacturing method
  • A kind of bioink containing stem cell exosome and its manufacturing method

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Embodiment Construction

[0047] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0048] 1. Experimental method

[0049] 1.1 Chondrogenic differentiation

[0050] (1) When the second-generation adipose-derived mesenchymal stem cells (ADSCs) grew to 90% density, 2 mL of 0.25% trypsin digestion solution was added, and the cell morphology was observed under an inverted microscope for 1-2 minutes (min). When the cells were found to shrink, round, gap widen, and suspend, an equal volume of complete medium was added to terminate the digestion.

[0051] (2) Transfer the cell suspension to a centrifuge tube, centrifuge at 800 rpm for 5 min.

[0052] (3) Count the cell suspension with an automatic cell counter, and adjust the cell density to 1.0 × 10 7...

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Abstract

The present invention provides a bio-ink and a manufacturing method thereof, as well as a bio-scaffold fabricated by 3D printing using the bio-ink, the bio-ink comprising methacrylic anhydride gelatin (gelatin methacryloyl, GelMA), oxidized hyaluronic acid (oxidized hyaluronic acid , OHA), dopamine conjugated hyaluronic acid (HA‑DA), cartilage decellularized matrix, and exosomes.

Description

technical field [0001] The invention belongs to the field of medical devices, and in particular relates to a bioink containing stem cell exosomes and a manufacturing method thereof. Background technique [0002] Articular cartilage is one of the important components of the locomotion system. It is connected to the surface of the bone end in the joint and has a smooth and elastic texture. Articular cartilage has the functions of lubricating joints, cushioning the vibration and impact generated by movement, improving the movement and shape matching between bones, and is very important for maintaining the normal movement function of joints. Cartilage damage can cause severe pain, joint swelling, decreased mobility, and degeneration of surrounding cartilage and subchondral bone, and even develop into osteoarthritis, seriously affecting the quality of life of patients and bringing heavy economic burdens to individuals and society. With the increasing enthusiasm of the Chinese pe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0662C12N5/0667C12N5/0655C12N2533/90C12N2533/54C12N2533/80
Inventor 敖英芳李琪胡晓青
Owner PEKING UNIV THIRD HOSPITAL
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