A gradient laminated composite supporting frame material based on bionic structures and its preparation method

Abstract

The invention relates to a kind of laminated gradient composite scaffold material based on the bionic structure and its preparation method. The said material has three or more layers of porous structure, comprising of hyaluronic acid, PLGA, PLA, collagen II and nano-hydroxyapatite (nano-HA) , beta- tricalcium phosphate ( beta-TCP). The upper layer is made by collagen II / hyaluronic acid or PLGA and PLA imitating the cartilage layer. With the counterfeit the calcified cartilage layer in the middle, it is one layer or multi- sublayer, made by nano-HA or beta-TCP with collagen II / hyaluronic acid or PLGA and PLA; the bottom is made of nano-HA or beta-TCP with collagen II or PLGA and PLA. From top to bottom, the content of inorganic material increases in its layers, about 0 to 60 mass percent of layers. The aperture of the scaffold material is 50 to 450 micron, with 70 to 93 percent porosity. The scaffold material made by this invention has an adjustable degradation rate, good mechanical and biocompatible properties, can adapt to culture with cartilage bone cells and compound with growth factor and small molecular or peptides, it can be used to repair cartilage simultaneously.

Description

technical field

[0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method and a technology of a tissue engineering laminated composite support material for osteochondral defect repair. Background technique

[0002] Articular cartilage defect is a very common disease, and its defect models include: I, less than 4 mm, a small area of ​​cartilage defect, which is a slight injury; II, greater than 4 mm, difficult to heal by itself, and requires external force; III, injury to the subchondral bone , because blood vessels in the bone will penetrate the subchondral bone and enter, leading to cartilage necrosis and fibrous tissue growth. The most common is the third type of injury. Due to the special tissue structure and biological characteristics of articular cartilage, although there are many known methods for treating articular cartilage defects, including: subchondral bone drilling, electrical stimulation, laser, drug an...