39 results about "Human cartilage" patented technology

The invention relates to novel trimeric costimulatory TNF family ligand-containing antigen binding molecules comprising three fusion polypeptides, each of the three fusion polypeptides comprising (a) an ectodomain of a costimulatory TNF family ligand selected from the group consisting of 4-1BBL, OX40L and GITRL or fragments thereof, (b) a trimerization domain, in particular a trimerization domain derived from human cartilage matrix protein (huCMP) of SEQ ID NO:1, and (c) a moiety capable of specific binding to a target cell antigen, and to methods of producing these molecules and to methods of using the same.

The invention discloses a preparation method of a novel high-strength micro-gel composite hydrogel. According to the invention, acrylamide and 2-acrylamide-dimethylpropane sulfonic acid are adopted as main monomers; N-methylol acrylamide is adopted as a functional monomer; reactive micro-gel containing hydroxymethyl is obtained through inverse emulsion polymerization; the micro-gel particles are dispersed into the water solution of acrylamide and 2-acrylamide-dimethylpropane sulfonic acid, and free radical polymerization is carried out, such that a micro-gel composite polymer is obtained; and the micro-gel composite polymer or a partially dehydrated micro-gel composite polymer is heated and is subjected to cross-linking, such that the micro-gel composite hydrogel is obtained. According to the invention, nano-clay is adopted as the cross-linking agent. The prepared composite hydrogel has higher mechanical strength, and high water absorption rate. The strength and water content of the hydrogel are similar to those of human cartilage tissues. Therefore, the hydrogel is an alternative material for cartilage tissue reparation.

This invention concerns an oligomeric receptor-ligand pair member complex which includes (i) an oligomeric core, said core comprising at least two chimeric proteins, said chimeric proteins comprising a first section including at least one domain forming part of a first member of a complementary binding pairand a second section comprising an oligomerising domain derived from an oligomer-forming coiled-coil protein, wherein formation of the oligomeric core occurs by oligomerisation at the oligomerising domain of the chimeric proteins; and (ii) at least two receptor-ligand pair member peptides derived from a receptor-ligand pair member peptide chain or a functional part thereof, wherein each receptor-ligand pair member peptide further comprises attached thereto a second member of said complementary binding pair capable of binding to the first complementary binding pair member as defined in (i). Each receptor-ligand pair member peptide is bound to the core via binding of the first and second members of the complementary binding pair. At least two of the receptor-ligand pair member peptides in the complex are derived from the same receptor-ligand pair member peptide chain. In one embodiment, the oligomerising domain in the second section in at least one of the chimeric proteins is derived from the pentamerisation domain of the human cartilage oligomeric matrix protein (COMP). The invention further concerns related pharmaceutical and diagnostic compositions and processes.

The invention relates to an induction method for promoting differentiation of human umbilical cord mesenchymal stem cells into chondrocytes, which comprises the following steps of: obtaining an umbilical cord tissue block, a primary culturing stem cells, subculturingstem cells and inducing stem cells differentiate to chondrocytes, wherein the induction for differentiation of stem cellsinto chondrocytes comprises the following steps of: resuspending human umbilical cord mesenchymal stem cells subcultured to a seventh generation by adopting a serum-free culture medium to prepare P7 generation stem cell suspension; inoculating P7 generation stem cell suspension into the serum-free culture medium containing a cartilage differentiation inducer for continuous culture; replacing the serum-free culture medium containing the cartilage differentiation inducer in a half amount every three days; obtaining chondrocytes after 14 days.The induction method can differentiate human umbilical cord mesenchymal stem cells into chondrocytes,which has important significance in repairing human cartilage tissues and treating bone injuries.

Medical devices having engineered mechanically functional cartilage from adult human mesenchymal stem cells and method for making same.

The invention discloses a high-strength degradable cartilage tissue engineering scaffold and a preparation method thereof. The high-strength degradable cartilage tissue engineering scaffold is formed by compounding chitosan and gelatine in weight ratio of 1:(0.01-2), wherein mass percent concentration of chitosan is 3-6%, and mass percent concentration of gelatine is 0.03-10%. The preparation method of the high-strength degradable cartilage tissue engineering scaffold is characterized in that natural biological materials such as chitosan and gelatine are adopted as raw materials, an in-situ precipitation and freeze-drying method is adopted for preparing a chitosan-gelatin compound porous scaffold which has excellent mechanical performance, controllable degradation rate and excellent biocompatibility, is not added with a crosslinking agent and is composed of pure natural biological materials. The chitosan-gelatin compound porous scaffold has the advantages that a degradation period can be regulated according to proportional relation between gelatine and chitosan, and growth rate matching probability of regenerated tissues is improved; and experiments verify that human cartilage cells can be well adhered and propagated on the surface of the materials.

The methods described herein are for conserving highly expanded cells that have functional properties such as potential for use in neotissue constructs. For example, highly expanded chondrocytes that can be used to construct neocartilage exhibiting functional properties similar to native articular cartilage. The methods and systems feature processes that form functional, human cartilage using cells that have been expanded to at least 1.5×10⁵ times or P3 or greater. This enables a large quantity of engineered cartilage implants to be produced from few cells.

The invention belongs to the technical field of forensic humans, and particularly relates to a method for determining human cartilage endochrome and application of the human cartilage endochrome in forensic medicine. An in-vitro cartilage section image is obtained by using a scanner, and image processing software is used for obtaining a pigmentation quantification method of an average gray value of an image. A linear model based on human cartilage endochrome quantification and age correlation is established. The method disclosed by the invention is simple and convenient to operate, low in costand high in accuracy, has very high forensic value, and can be popularized in forensic practice.

The invention relates to application of human cartilage glycoprotein (YKL-40) as a glioblastoma biomarker. The glioblastoma (Glioblastoma, GBM) patient is low in survival rate and poor in prognosis. Effective biomarker and monitoring method are absent at present. The invention finds that the overall YKL-40 meso-position expression is obviously increased (Fold change=9.483, P<0.0001) by analyzing the YKL-40 mRNA gene expression data of the glioblastoma patient in a TCGA (The Cancer Genome Atlas) database. 45 IV-stage Chinese people glycoprotein clinic samples are further collected, and after qPCR process is adopted for quantitatively analyzing the YKL-40 expression and the prognosis correlation analysis is performed, the YKL-40 mRNA transcriptional level of 41 patients is obviously increased and the patients are short in lifetime and poor in prognosis. The invention analyzes and verifies that the YKL-40 is closely related to the lifetime and poor prognosis, the YKL-40 can be used as a potential glioblastoma biomarker, and a qPCR or protein quantitative monitoring method can be utilized to indicate the lifetime and prognosis of the glioblastoma patient.

Nucleic acid sequences for HC gp-39L are provided. Methods of detecting altered expression of tissue remodeling proteins and diagnosing tissue remodeling disorders are also provided.

The invention relates to the technical field of tissue engineering, and in particular relates to an in-vitro construction method of epiphyseal plate cartilage. The in-vitro construction method of the epiphyseal plate cartilage comprises the following steps: (1) culturing human cartilage cell line cells or human bone marrow stem cells till cell monolayers are formed, carrying out trypsinization, washing and resuspension to obtain a cell suspension, and carrying out counting for later use; (2) cutting a pretreated bone matrix gelatin material into a cylinder with the diameter smaller than the diameter of each culture hole, drilling a pore passage running through the two bottom surfaces of the cylinder in the cylinder, carrying out sterilization treatment, and fixing the cut bone matrix gelatin material in the culture holes in a suspension manner; and (3) implanting the cell suspension obtained in the step (1) to be below the bone matrix gelatin material from the pore passage, enabling the liquid level to wet the bone matrix gelatin material, and carrying out culturing to obtain the epiphyseal plate cartilage. With the adoption of the technical scheme, a culture system of C28/I2 cells and an in-vitro construction method of the epiphyseal plate cartilage by adopting the culture system are established, and therefore, the problem that the seed cells are difficultly available is solved; the cells are stable in character and low in culturing cost.

A method for differentiation of osteoarthritis from rheumatoid arthritis and non-disease conditions in a sample, comprising measuring in the sample the concentration of a peptide comprising the 15 amino acid sequence (SEQ ID NO: 1) of the human cartilage intermediate layer protein 2.

The invention discloses a preparation method for a stem cell extract-rich degradable biofilm for wound repair. The preparation method comprises the following steps: adding extracted bone marrow into a mesenchymal stem cell culture solution, discarding supernatant after centrifugation, then performing inoculation in a culture dish at 1*109/mL, performing culturing in an incubator, and inoculating third-generation mesenchymal stem cells into a fiberboard laid with a fibrin film to prepare a mesenchymal stem cell-fibrin film. According to the preparation method, mesenchymal stem cells are separated and cultured, the fibrin film is used as a stent material, and the in-vitro cultured mesenchymal stem cells and the fibrin film are combined and transplanted to a cartilage damaged part to achieve the effect of repairing a damaged cartilage. The mesenchymal stem cells can be better limited by the fibrin film to be grown and reproduced on the surface of a cartilage damaged wound so as to play an important role in a cartilage wound repair process more effectively and play a certain guiding role in human cartilage damage repair.

The invention discloses a cartilage tissue repair membrane and a preparation method thereof. The cartilage tissue repair membrane is mainly made from, by weight: 25-50 parts of collagen, 13-28 parts of glycosaminoglycans, 11-24 parts of hyaluronic acid, 8-24 parts of chitosan, 4-14 parts of fibronectin, 10-32 parts of keratin, 5-15 parts of membrane fiber, 14-26 parts of sorbitol, 10-25 parts of sodium lauryl sulfate, 14-36 parts of cellulose gum, 10-26 parts of benzyl alcohol, and 35-65 parts of double distilled water. Compared with the prior art, the invention has the advantages that the prepared cartilage tissue repair membrane has water content 60-80% and meets the requirement for repairing human cartilage tissues; the preparation method of the cartilage tissue repair membrane is simple, quick and low in cost.