2 results about "Human cartilage" patented technology

It is an object of the present invention a method to obtain a cartilage cell population enriched in cartilage progenitor cells (CPCs), said method comprising culturing said cells in culture at a density between 10 and 500 cells / cm2. In a further aspect, it is here claimed a population of cartilage cells enriched in CPCs obtainable by means of said method, and their use in the treatment of chondral lesions or joint degeneration.

This invention relates to a method for constructing a humanized pig model of type II collagen and its application, belonging to the field of gene modification technology. The invention first provides a CRISPR-Cas9 system for targeted editing of the porcine type II collagen α1 gene. Using CRISPR / Cas9-mediated homologous recombination, the porcine COL2A1 gene is orally replaced with the human COL2A1 gene. Then, using somatic cell cloning technology, a humanized pig model of type II collagen is constructed, allowing the pig to produce cartilage tissue more similar to that of humans, reducing post-transplant immune rejection. This is expected to fundamentally solve the problem of human cartilage tissue shortage and has significant implications for the treatment of articular cartilage damage, providing a better and more suitable transplant material for the treatment of articular cartilage damage.