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31 results about "Mrna gene expression" patented technology

The role of mRNA in Gene expression Gene expression refers to the conversion of genetic information from genes via messenger RNA (mRNA) to proteins.

Eukaryotic expression vector for producing recombinant protein by using CHO cells, and system

The invention relates to the field of genetic engineering, and specifically discloses a gene expression system for producing recombinant protein by using CHO cells, and an eukaryotic expression vector, wherein the eukaryotic expression vector comprises a GS expression unit, and the GS expression unit comprises a TK promoter gene sequence aligned along a 5' to 3' direction, a glutamine synthetase gene sequence and a SV40polyA gene sequence. The expression system comprises the eukaryotic expression vector and CHO cells. With the expression system, integration and efficient expression of exogenous gene in CHO cell genome can be achieved, and broad application prospects are provided in the field of protein.
Owner:SHANGHAI HENLIUS BIOTECH INC +1

Ovarian cancer molecular typing prediction system

ActiveCN109360604AHelps growHelp improve clinical treatment planProteomicsGenomicsPrincipal component analysisError reporting
The invention provides a ovarian cancer molecular typing prediction system. The system mainly comprises the following steps of step1, using an ovarian cancer mRNA gene expression characteristic data extraction module: acquiring ovarian cancer gene expression data; step2, for all gene expression data, using a preprocessing. scale method in skleam to carry out standardized processing, and accordingto a formula which is Z-scroce=(x-mu) / S<2>, and processing each mRNA expression spectrum data into data submitting to normal distribution with the mean value of 0 and the variance of 1; step3, selecting main characteristic gene data: using principal component analysis (PCA) and a Filter characteristic selection method; step4, using a BP neural network to carry out genetic data training model withN characteristics; and step5, using a certain amount of samples to carry out callback program verification. In the invention, through an ovarian cancer pathological section, automatic machine identification and error reporting can be realized, and rapid and accurate ovarian cancer molecular typing prediction is achieved; and the system is used to carry out ovarian cancer molecular typing prediction so that a clinical treatment plan can be improved.
Owner:NANCHANG ROYO BIOTECH CO LTD

Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis

The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.
Owner:DOW AGROSCIENCES LLC +1

Kit and method for detecting expression level of TYMS (Thymidylate Synthetase) mRNA (messenger Ribonucleic Acid)

The invention discloses a kit and method for detecting the expression level of TYMS (Thymidylate Synthetase) mRNA (messenger Ribonucleic Acid). The kit comprises a standard substance and an internal reference gene real-time quantitative PCR (Polymerase Chain Reaction) system, wherein the standard substance is used for making a standard curve. Through the method, the expression level of TYMS can be accurately, rapidly and quantitatively determined. If the method and the kit, disclosed by the invention, are applied in clinic, doctors can rationally select fluorine drugs for treatment.
Owner:周宏灏

Gene expression system for producing recombinant protein by using CHO cells and eukaryotic expression vector

The invention relates to the field of biotechnology, and in particular, relates to a gene expression system for producing a recombinant protein by using CHO cells and a eukaryotic expression vector. The invention provides the pHLX201 eukaryotic expression vector including a GS expression unit, the GS expression unit has the sequence comprising an SV40L promoter gene sequence and a glutamine synthetase gene sequence which are arrayed successively in a direction from 5' to 3', wherein the SV40L promoter gene sequence is shown in SEQ ID NO:2, and the glutamine synthetase gene sequence is shown in SEQ ID NO:3. The expression system provided by the invention can realize integration and efficient expression of exogenous genes in a CHO cell genome. The preparation method of the system mainly includes that the eukaryotic expression vector is constructed, is suitable for transient expression of a target gene in the CHO cells and is suitable for screening target gene high-expression cell lines. The CHO expression system can be used for realizing integration and expression of the recombinant protein, a monoclonal antibody and the like in the CHO cells, and has wide application prospects.
Owner:SHANGHAI HENLIUS BIOTECH INC

Reference gene for barley gene expression researches as well as application of reference gene

The invention discloses a reference gene for barley gene expression researches as well as application of the reference gene. The reference gene is a gene segment of a ubiquitin-conjugating enzyme E2, an elongation factor EF2 and / or a tubulin beta-tubulin 6, wherein a nucleotide sequence of the gene segment of the ubiquitin-conjugating enzyme E2 is shown as SEQ ID NO.1, a nucleotide sequence of the gene segment of the elongation factor EF2 is shown as SEQ ID NO.2, and a nucleotide sequence of the gene segment of the tubulin beta-tubulin 6 is shown as SEQ ID NO.3. According to the reference gene, the detection accuracy of a barley related gene (such as low nitrogen stress and drought stress) expression can be improved, and the reference gene is large in expression quantity and stable in expression, and has a Ct value of 20 to 30.
Owner:SHANGHAI ACAD OF AGRI SCI

Nucleic acid molecule and method of targeting gene expression to gliomas

There is presently provided a nucleic acid molecule comprising a glial-specific promoter; a coding sequence for a transgene; and a plurality of miRNA target sites. Each miRNA target site binds an miRNA that is down-regulated in .a glioma cell compared to a normal glial cell, and the glial-specific promoter and the plurality of miRNA target sites are both operably linked to the coding sequence for the transgene.
Owner:AGENCY FOR SCI TECH & RES

Enhanced nucleic acid constructs for eukaryotic gene expression

The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery.
Owner:DNA2 0

Specific primers for detection of mRNA expression level of Acidithiobacillus thiooxidans sor gene

The invention provides specific primers for detection of mRNA expression level of an Acidithiobacillus thiooxidans sor gene. The nucleotide sequences are as follows: an upstream primer sequence of 5'-AAGCCCGTGCCTAAAGTG-3', and a downstream primer sequence of 5'-CTGCCATAGTTGGTGTTGT-3'. The specific primers can detect the transcription level of Acidithiobacillus thiooxidans sor gene, and hace the advantages of high sensitivity and good specificity in detecting mRNA gene expression level. Through the real-time monitoring of mRNA expression level of sor gene in sulfur oxidation process, the primers are used to explain the sulfur oxidation mechanism of Acidithiobacillus thiooxidans.
Owner:CENT SOUTH UNIV

IgA nephropathy diagnosis marker combination and application thereof

The invention discloses an IgA nephropathy diagnosis marker combination and application thereof. In the first aspect, the application provides: a quantitative detection gene ADIPOR2; CDK14; at least one of CYB561D1 and CYB561D2; DOCK10; IL33; at least one of PCDH18 and PCDH17; at least one of PLEKHG2 and PLEKHG3; and at least one of RASDRF2 and RASGRF1. The invention discloses application of a reagent of VIL1 in preparation of a diagnostic kit for IgA nephropathy. According to the application, a combination of the nine genes is screened from total mRNA gene expression group data, wherein whether a subject suffers from the IgA nephropathy or not can be efficiently and accurately diagnosed by performing quantitative detection on the subject based on the nine genes.
Owner:SHENZHEN LUWEI BIOTECHNOLOGY (BIOMANIFOLD TECH CO) LTD

Application of YKL-40 as glioblastoma biomarker

The invention relates to application of human cartilage glycoprotein (YKL-40) as a glioblastoma biomarker. The glioblastoma (Glioblastoma, GBM) patient is low in survival rate and poor in prognosis. Effective biomarker and monitoring method are absent at present. The invention finds that the overall YKL-40 meso-position expression is obviously increased (Fold change=9.483, P<0.0001) by analyzing the YKL-40 mRNA gene expression data of the glioblastoma patient in a TCGA (The Cancer Genome Atlas) database. 45 IV-stage Chinese people glycoprotein clinic samples are further collected, and after qPCR process is adopted for quantitatively analyzing the YKL-40 expression and the prognosis correlation analysis is performed, the YKL-40 mRNA transcriptional level of 41 patients is obviously increased and the patients are short in lifetime and poor in prognosis. The invention analyzes and verifies that the YKL-40 is closely related to the lifetime and poor prognosis, the YKL-40 can be used as a potential glioblastoma biomarker, and a qPCR or protein quantitative monitoring method can be utilized to indicate the lifetime and prognosis of the glioblastoma patient.
Owner:BEIJING TRICISIONBIO THERAPEUTICS INC

Ubi1 intron sequence capable of enhancing gene expression and applications

The invention discloses a ubi1 intron sequence capable of enhancing gene expression and applications. According to the sequence and applications, deletion analysis is conducted on a ubiquitin first intron from maize, the novel intron sequence capable of enhancing the foreign gene expression is obtained, the length of the intron sequence is 872bp, the adenine-thymine (AT) content is 61.0%, the gene expression can be improved by 13 times, and compared with the full-length ubiquitin first intron, the intron sequence improves the foreign gene expression ability by 30%. The intron obtained through the sequence can be used for constructing an efficient plant expression vector and improving the foreign gene expression level of a transgenic plant. According to the sequence and applications, the plant expression vector containing the modified intron is constructed, and the intron is used for cultivating of the transgenic plant to improve the expression of other genes in the transgenic plant.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI

shRNA (short hairpin ribonucleic acid) capable of inhibiting IRS (insulin receptor substrate) 2 gene expression and application thereof

The invention discloses shRNA (short hairpin ribonucleic acid) capable of inhibiting IRS (insulin receptor substrate) 2 gene expression and application thereof, belonging to the technical field of gene engineering. The nucleotide sequence of the shRNA is shown in SEQ ID No.1. The invention also provides an interference vector of shRNA capable of inhibiting IRS 2 gene expression. Through cloning the compared and analyzed pig source IRS 2 gene conserved sequence, interference fragments with restriction enzyme cutting site BamH I and Hind III are synthesized, the interference fragments are connected onto a plasmid vector linearized by BamH I and Hind III, and effective interference vectors are screened out. The shRNA and the interference vector thereof can effectively inhibit IRS2 gene expression, affects cell glucolipid metabolism, and lays a foundation for construction of type 2 diabetic pig model.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Replication system and application thereof in gene expression

The invention provides a method for obtaining a target gene expression product, which comprises expressing a recombinant RNA molecule in a host bacterium and culturing the host bacterium in the presence of a phage RNA replicase and under conditions suitable for expression of the target gene; wherein the recombinant RNA molecule comprises a 5' replication recognition sequence and a 3' replication recognition sequence which are recognizable by a phage RNA replicase and perform an RNA replication process, and a target gene sequence arranged between the 5' replication recognition sequence and the3' replication recognition sequence. The invention also provides s recombinant bacterium capable of expressing the recombinant RNA molecule. The method can modify engineering bacteria from the level of directly increasing the RNA expression quantity so as to promote the expression of the target gene.
Owner:TSINGHUA UNIV

Immunoglobulin A nephropathy T cell diagnostic marker

PendingCN114324887AEfficient and accurate diagnosisImprove featuresMicrobiological testing/measurementBiostatisticsGATA3Cellular immunity
The invention discloses an immunoglobulin A nephropathy T cell diagnostic marker. On the first aspect, the invention provides application of a reagent for quantitatively detecting at least one of the following markers in preparation of the diagnostic kit for IgA nephropathy: CCR3, CD4, CD8A, GATA3, GZMA, HDAC7, RORA and VEGFC. According to the application disclosed by the embodiment of the invention, the application at least has the following beneficial effects that the pathogenesis of the immunoglobulin A nephropathy is related to five gene axes (Axis), screening is carried out from related mRNA gene expression data based on a specific gene or protein of a T cell from the T cell immune axis (T Cell Immunity Axis) to obtain the eight markers, and the immunoglobulin A nephropathy can be used for detecting the immunoglobulin A nephropathy. Whether a subject suffers from IgA nephropathy or not can be efficiently and accurately diagnosed through quantitative detection on the basis of at least one of the eight markers, and good specificity and sensitivity are achieved.
Owner:SHENZHEN LUWEI BIOTECHNOLOGY (BIOMANIFOLD TECH CO) LTD

Shrna sequence and application thereof for inhibiting expression of mouse macf1 gene

The invention discloses an shRNA sequence for inhibiting mouse MACF1 gene expression and application thereof, which is used to solve the technical problem of low efficiency of the existing shRNA sequence for inhibiting mouse MACF1 gene expression. The technical solution is to clone the shRNA sequence that inhibits the expression of the mouse MACF1 gene into the pGLV3 lentiviral vector to obtain a recombinant lentiviral vector containing the shRNA sequence; the obtained recombinant vector is used to transfect mouse cells to achieve inhibition Purpose of MACF1 gene expression in cells. The shRNA sequence mentioned above can obviously inhibit the expression of MACF1 gene at mRNA and protein levels in mouse preosteoblasts. The proliferation, migration and differentiation functions of preosteoblasts were inhibited by inhibiting the expression of MACF1 in mouse preosteoblasts. The inhibition rate of the present invention at the protein level reaches over 90%, and the inhibition rate at the gene level reaches 81%.
Owner:NORTHWESTERN POLYTECHNICAL UNIV

Multi-gene expression and silencing system controlled by heat shock protein gene promoter and tetracycline gene promoter

The invention discloses a multi-gene expression and silencing system controlled by a heat shock protein gene promoter and a tetracycline gene promoter. The heat shock protein gene promoter HSPP and the tetracycline gene promoter TETp in the same plasmid system do not influence each other and independently control expression of downstream target genes or shRNAs (small hairpin ribonucleic acids) respectively, expression of single gene or shRNA can be controlled manually, and co-expression of two genes or shRNAs can be also controlled manually. The multi-gene expression and silencing system provides conditions for multi-gene specificity coordination control, solves the problem of low expression efficiency caused by gene co-expression implemented with a multi-plasmid cotransfection method at present, adopts a lentivirus carrying system to realize efficient expression of selective genes or shRNAs in cells having difficulty in transfection, meets various experimental requirements, provides a multi-gene target control platform, and provides more possibilities for gene therapy.
Owner:CHONGQING UNIV

shRNA inhibiting expression of rabbit Deptor gene, lentivirus expression vector and construction method and application of lentivirus expression vector

The invention discloses shRNA inhibiting the expression of a rabbit Deptor gene, a lentivirus expression vector and a construction method and application of the lentivirus expression vector. The shRNAis pSicoR-shRNA-508 and is composed of a siRNA positive-sense strand, a loop, a siRNA antisense chain and a termination signal. shRNA can effectively and stably inhibit the expression of the rabbit Deptor gene, the influence and action mechanism of the Deptor gene on the exogenous gene expression efficiency can be studied by inhibiting the Deptor gene expression, the influence of the Deptor geneon rabbit embryonic stem cell multfunctionality maintenance and autophagy pathway related gene expression can be further verified, and a foundation is laid for stable rabbit embryonic stem cell establishment.
Owner:GUANGXI UNIV

A porcine ucoe regulatory element fragment that enhances exogenous gene expression

The invention belongs to the technical field of gene engineering, and particularly relates to a porcine UCOE regulatory element fragment for enhancing exogenous gene expression. The base sequence of the UCOE regulatory element fragment is shown in a sequence table. With respect to exogenous protein expression, the regulatory element fragment can enhance GFP expression by constructing recombinant plasmid expression vector pCpG-Mini-GFP-UCOE and transfecting HEK293T cells. According to the invention, with a human 1.5 kb UCOE fragment used as a control, a porcine UCOE fragment is cut into fragments with different lengths by an enzyme digestion method and an RT-PCR method; different expression vectors are constructed, and transfected into HEK293T cells; the relative expression level of gene GFP is reported by real-time fluorescence quantification PCR and Western blot technique detection, and the optimal UCOE fragment sequence for enhancing exogenous gene expression is screened, which is the gene sequence claimed in the invention.
Owner:HENAN AGRICULTURAL UNIVERSITY

Kit for detecting PDL-1 mRNA gene expression quantity and application thereof

The invention relates to a kit for detecting a PDL-1 mRNA gene expression quantity. The kit mainly comprises specific primers and a PCR reaction reagent, and is characterized in that the sequences ofthe specific primers are as shown in the description; a PDL-1 upstream primer is as shown in the SEQ ID NO.1 and is 5'-AAGCACACGTGCCAAAGAATC-3'; and a PDL-1 downstream primer is as shown in the SEQ IDNO.2 and is 5'-TCTCGACGACGACGTTTCC-3'. The method can easily, quickly, accurately and reliably detect the gene expression quantity of the PDL-1 mRNA.
Owner:宿迁市第一人民医院

Pichia stipitis gene expression system and its construction and application

The present invention relates to a Pichia stipitis gene expression system and its construction and application, including a novel expression vector, which is circular and operably connected with the following elements sequentially from 5'-3': pMD19-Tsimple plasmid backbone, rDNA homologous recombination sequence, exogenous gene expression cassette and screening marker gene expression cassette; the exogenous gene expression cassette includes promoter, exogenous gene insertion restriction site and transcription terminator in sequence from upstream to downstream; the screening The marker gene expression cassette includes a promoter, an antibiotic resistance gene, and a transcription terminator. The yeast capable of utilizing xylose is Pichia stipitis. The expression vector of the present invention can realize integrated stable expression in Pichia stipitis, and has important significance for basic theoretical research and product development of Pichia stipitis.
Owner:JIANGNAN UNIV

A kind of eukaryotic expression vector and system using CHO cell to produce recombinant protein

The invention relates to the field of genetic engineering, and specifically discloses a gene expression system and a eukaryotic expression vector for producing recombinant proteins using CHO cells. The eukaryotic expression vector includes a GS expression unit, and the GS expression unit includes 5' to 3' TK promoter gene sequence, glutamine synthetase gene sequence and SV40 poly A gene sequence arranged in sequence in the direction; the expression system of the present invention includes eukaryotic expression vector and CHO cell, and this expression system can realize exogenous gene in CHO cell genome The integration and high-efficiency expression in the protein have broad application prospects in the field of protein preparation.
Owner:SHANGHAI HENLIUS BIOTECH INC +1

A Saccharomyces cerevisiae gene expression system and its construction and application

The invention relates to an integrated Saccharomyces cerevisiae gene expression system which comprises an expression vector, wherein the expression vector sequentially comprises the following operable elements from 5'-3': a pMD19-Tsimple plasmid framework, an rDNA homologous recombinant sequence, an exogenous gene expression cassette and a selective marker gene expression cassette; the exogenous gene expression cassette sequentially comprises a promoter, an exogenous gene insertion enzyme digestion site and a transcription terminator from upstream to downstream; and the selective marker gene expression cassette comprises a promoter, an antibiotic resistance gene and a transcription terminator. The yeast is Saccharomyces cerevisiae. The expression vector can implement integrated stable expression in the Saccharomyces cerevisiae, and has important meanings for fundamental research and product development of the Saccharomyces cerevisiae.
Owner:JIANGNAN UNIV

New gene MDL1, and quantitative detection method thereof

The invention discloses a human mitochondrial new gene MDL1, the full-length nucleotide sequence of a gene MDL 1 AS, and the quantitative detection method of the expression quantity thereof, and belongs to the field of biotechnology. The nucleotide sequence of the gene MDL1 is shown as: 1) a nucleotide sequence shown in SEQ ID NO. 1; or 2) a nucleotide sequence obtained through replacing, deleting or inserting one or several nucleotides in the nucleotide sequence shown in SEQ ID NO. 1. The nucleotide sequence of the gene MDL 1 AS is shown as: 1) a nucleotide sequence shown in SEQ ID NO. 2; or 2) a nucleotide sequence obtained through replacing, deleting or inserting one or several nucleotides in the nucleotide sequence shown in SEQ ID NO. 2. The gene MDL1 and the gene MDL 1 AS are key genes for regulating the gene expression of mitochondrial genes. The mitochondrial energy supply is the basis for various life activities. The present invention verifies that, the expression of the gene MDL1 significantly changes in various tumor cells, so that the gene MDL1 has very important clinical detection and therapeutic value.
Owner:NANKAI UNIV

siRNA capable of inhibiting mat2a gene expression and its application

The invention discloses siRNA capable of inhibiting MAT2A gene expression and application thereof. For the siRNA MAT2A-1 that inhibits the expression of the MAT2A gene of the present invention, its sense strand is shown in SEQ ID NO.2, and the antisense strand is as shown in SEQ ID NO.3; for the siRNA MAT2A-2 that inhibits the expression of the MAT2A gene, its sense strand As shown in SEQ ID NO.4, the antisense strand is shown in SEQ ID NO.5. The lentiviral expression vector for inhibiting the expression of the MAT2A gene constructed based on the siRNA that inhibits the expression of the MAT2A gene is combined with the lentivirus that overexpresses the MAT1A gene (its nucleotide sequence is shown in the 256th-1443rd base of SEQ ID NO.1) Vector transfection of liver cancer cells successfully inhibits hepatocellular carcinoma angiogenesis, migration and invasion, and lung metastasis, which can be further applied to the preparation of drugs for the treatment of liver cancer, and has high theoretical and application value.
Owner:李家平 +3

Application of ein2 protein in repressing gene expression

The invention discloses an application of EIN2 protein in inhibiting gene expression. Cytoplasm in EIN2 protein acts with 3'UTR of EBF1mRNA, and is positioned at P-body together with protein such as EIN5, so that the translation process of EBF1mRNA can be inhibited. Due to expression of EIN2 protein in plants, particularly high-class plants, the translation process of any gene with EBF1 3'UTR can be inhibited; only by combining a coding sequence of a target gene with EBF1 3'UTR, expression of the gene can be horizontally started or ended after transcription by expressing EIN2 under certain conditions.
Owner:PEKING UNIV

Gene expression down-regulated vector of cell specificity ndrg2

InactiveCN101565717BDown-regulation effect is obviousEasy to observeVector-based foreign material introductionRNA polymerase IIPreproinsulin
The invention discloses a gene expression down-regulated vector of cell specificity ndrg2, which comprises an RNAi transgenic vector. A preproinsulin promoter is connected to the RNAi transgenic vector, a miR accessed locus is inserted to the down stream of the preproinsulin promoter, and the miR accessed locus is connected with a miRNA sequence aiming at down-regulated ndrg2 genes. The constructed RNAi transgenic vector adopts the preproinsulin promoter relied by RNA polymerase II to promote the downstream miRNA sequence by specific insulin beta cells of the preproinsulin promoter, and the specific insulin beta cells promote RNA interference aiming at ndrg2 genes to down-regulate the expression thereof; and the vector is used for constructing an animal model and researching the functionsof the ndrg2 genes of the insulin beta cells.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY

A method for screening and functional analysis of oncogenes related to non-small cell lung cancer

ActiveCN106778066BBiostatisticsProteomicsMrna gene expressionMolecular pathogenesis
The invention discloses a method for screening and functionally analyzing related oncogenes of non-small-cell lung cancer. The method includes steps of searching mRNA [messenger RNA (ribonucleic acid)] expression chip results related to the NSCLC (non-small-cell lung cancer) from GEO (gene expression omnibus) databases http: / / www.ncbi.nlm.nich.gov / geo / and acquiring mRNA expression results by the aid of GEO2R databases; searching identical mRNA gene expression results in two research results by the aid of Venn diagrams; functionally analyzing gene enrichment by the aid of bioinformatics technologies. The method has the advantages that mRNA differential expression genes are downloaded by the aid of the diversified online databases, the common differential expression genes in different research series are searched and are subjected to bioinformatics analysis, and accordingly significant exploration and bases can be provided for tumor marker screening, molecular pathogenesis and the like of the NSCLC.
Owner:THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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