31 results about "Mrna gene expression" patented technology

The invention relates to the field of genetic engineering, and specifically discloses a gene expression system for producing recombinant protein by using CHO cells, and an eukaryotic expression vector, wherein the eukaryotic expression vector comprises a GS expression unit, and the GS expression unit comprises a TK promoter gene sequence aligned along a 5' to 3' direction, a glutamine synthetase gene sequence and a SV40polyA gene sequence. The expression system comprises the eukaryotic expression vector and CHO cells. With the expression system, integration and efficient expression of exogenous gene in CHO cell genome can be achieved, and broad application prospects are provided in the field of protein.

The invention provides a ovarian cancer molecular typing prediction system. The system mainly comprises the following steps of step1, using an ovarian cancer mRNA gene expression characteristic data extraction module: acquiring ovarian cancer gene expression data; step2, for all gene expression data, using a preprocessing. scale method in skleam to carry out standardized processing, and accordingto a formula which is Z-scroce=(x-mu)/S<2>, and processing each mRNA expression spectrum data into data submitting to normal distribution with the mean value of 0 and the variance of 1; step3, selecting main characteristic gene data: using principal component analysis (PCA) and a Filter characteristic selection method; step4, using a BP neural network to carry out genetic data training model withN characteristics; and step5, using a certain amount of samples to carry out callback program verification. In the invention, through an ovarian cancer pathological section, automatic machine identification and error reporting can be realized, and rapid and accurate ovarian cancer molecular typing prediction is achieved; and the system is used to carry out ovarian cancer molecular typing prediction so that a clinical treatment plan can be improved.

The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

The invention discloses a kit and method for detecting the expression level of TYMS (Thymidylate Synthetase) mRNA (messenger Ribonucleic Acid). The kit comprises a standard substance and an internal reference gene real-time quantitative PCR (Polymerase Chain Reaction) system, wherein the standard substance is used for making a standard curve. Through the method, the expression level of TYMS can be accurately, rapidly and quantitatively determined. If the method and the kit, disclosed by the invention, are applied in clinic, doctors can rationally select fluorine drugs for treatment.

The invention relates to the field of biotechnology, and in particular, relates to a gene expression system for producing a recombinant protein by using CHO cells and a eukaryotic expression vector. The invention provides the pHLX201 eukaryotic expression vector including a GS expression unit, the GS expression unit has the sequence comprising an SV40L promoter gene sequence and a glutamine synthetase gene sequence which are arrayed successively in a direction from 5' to 3', wherein the SV40L promoter gene sequence is shown in SEQ ID NO:2, and the glutamine synthetase gene sequence is shown in SEQ ID NO:3. The expression system provided by the invention can realize integration and efficient expression of exogenous genes in a CHO cell genome. The preparation method of the system mainly includes that the eukaryotic expression vector is constructed, is suitable for transient expression of a target gene in the CHO cells and is suitable for screening target gene high-expression cell lines. The CHO expression system can be used for realizing integration and expression of the recombinant protein, a monoclonal antibody and the like in the CHO cells, and has wide application prospects.

The invention discloses a reference gene for barley gene expression researches as well as application of the reference gene. The reference gene is a gene segment of a ubiquitin-conjugating enzyme E2, an elongation factor EF2 and/or a tubulin beta-tubulin 6, wherein a nucleotide sequence of the gene segment of the ubiquitin-conjugating enzyme E2 is shown as SEQ ID NO.1, a nucleotide sequence of the gene segment of the elongation factor EF2 is shown as SEQ ID NO.2, and a nucleotide sequence of the gene segment of the tubulin beta-tubulin 6 is shown as SEQ ID NO.3. According to the reference gene, the detection accuracy of a barley related gene (such as low nitrogen stress and drought stress) expression can be improved, and the reference gene is large in expression quantity and stable in expression, and has a Ct value of 20 to 30.

There is presently provided a nucleic acid molecule comprising a glial-specific promoter; a coding sequence for a transgene; and a plurality of miRNA target sites. Each miRNA target site binds an miRNA that is down-regulated in .a glioma cell compared to a normal glial cell, and the glial-specific promoter and the plurality of miRNA target sites are both operably linked to the coding sequence for the transgene.

The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery.

The invention provides specific primers for detection of mRNA expression level of an Acidithiobacillus thiooxidans sor gene. The nucleotide sequences are as follows: an upstream primer sequence of 5'-AAGCCCGTGCCTAAAGTG-3', and a downstream primer sequence of 5'-CTGCCATAGTTGGTGTTGT-3'. The specific primers can detect the transcription level of Acidithiobacillus thiooxidans sor gene, and hace the advantages of high sensitivity and good specificity in detecting mRNA gene expression level. Through the real-time monitoring of mRNA expression level of sor gene in sulfur oxidation process, the primers are used to explain the sulfur oxidation mechanism of Acidithiobacillus thiooxidans.

The invention discloses an IgA nephropathy diagnosis marker combination and application thereof. In the first aspect, the application provides: a quantitative detection gene ADIPOR2; CDK14; at least one of CYB561D1 and CYB561D2; DOCK10; IL33; at least one of PCDH18 and PCDH17; at least one of PLEKHG2 and PLEKHG3; and at least one of RASDRF2 and RASGRF1. The invention discloses application of a reagent of VIL1 in preparation of a diagnostic kit for IgA nephropathy. According to the application, a combination of the nine genes is screened from total mRNA gene expression group data, wherein whether a subject suffers from the IgA nephropathy or not can be efficiently and accurately diagnosed by performing quantitative detection on the subject based on the nine genes.

The invention relates to application of human cartilage glycoprotein (YKL-40) as a glioblastoma biomarker. The glioblastoma (Glioblastoma, GBM) patient is low in survival rate and poor in prognosis. Effective biomarker and monitoring method are absent at present. The invention finds that the overall YKL-40 meso-position expression is obviously increased (Fold change=9.483, P<0.0001) by analyzing the YKL-40 mRNA gene expression data of the glioblastoma patient in a TCGA (The Cancer Genome Atlas) database. 45 IV-stage Chinese people glycoprotein clinic samples are further collected, and after qPCR process is adopted for quantitatively analyzing the YKL-40 expression and the prognosis correlation analysis is performed, the YKL-40 mRNA transcriptional level of 41 patients is obviously increased and the patients are short in lifetime and poor in prognosis. The invention analyzes and verifies that the YKL-40 is closely related to the lifetime and poor prognosis, the YKL-40 can be used as a potential glioblastoma biomarker, and a qPCR or protein quantitative monitoring method can be utilized to indicate the lifetime and prognosis of the glioblastoma patient.

The invention discloses a ubi1 intron sequence capable of enhancing gene expression and applications. According to the sequence and applications, deletion analysis is conducted on a ubiquitin first intron from maize, the novel intron sequence capable of enhancing the foreign gene expression is obtained, the length of the intron sequence is 872bp, the adenine-thymine (AT) content is 61.0%, the gene expression can be improved by 13 times, and compared with the full-length ubiquitin first intron, the intron sequence improves the foreign gene expression ability by 30%. The intron obtained through the sequence can be used for constructing an efficient plant expression vector and improving the foreign gene expression level of a transgenic plant. According to the sequence and applications, the plant expression vector containing the modified intron is constructed, and the intron is used for cultivating of the transgenic plant to improve the expression of other genes in the transgenic plant.

The invention discloses shRNA (short hairpin ribonucleic acid) capable of inhibiting IRS (insulin receptor substrate) 2 gene expression and application thereof, belonging to the technical field of gene engineering. The nucleotide sequence of the shRNA is shown in SEQ ID No.1. The invention also provides an interference vector of shRNA capable of inhibiting IRS 2 gene expression. Through cloning the compared and analyzed pig source IRS 2 gene conserved sequence, interference fragments with restriction enzyme cutting site BamH I and Hind III are synthesized, the interference fragments are connected onto a plasmid vector linearized by BamH I and Hind III, and effective interference vectors are screened out. The shRNA and the interference vector thereof can effectively inhibit IRS2 gene expression, affects cell glucolipid metabolism, and lays a foundation for construction of type 2 diabetic pig model.

The invention discloses an immunoglobulin A nephropathy T cell diagnostic marker. On the first aspect, the invention provides application of a reagent for quantitatively detecting at least one of the following markers in preparation of the diagnostic kit for IgA nephropathy: CCR3, CD4, CD8A, GATA3, GZMA, HDAC7, RORA and VEGFC. According to the application disclosed by the embodiment of the invention, the application at least has the following beneficial effects that the pathogenesis of the immunoglobulin A nephropathy is related to five gene axes (Axis), screening is carried out from related mRNA gene expression data based on a specific gene or protein of a T cell from the T cell immune axis (T Cell Immunity Axis) to obtain the eight markers, and the immunoglobulin A nephropathy can be used for detecting the immunoglobulin A nephropathy. Whether a subject suffers from IgA nephropathy or not can be efficiently and accurately diagnosed through quantitative detection on the basis of at least one of the eight markers, and good specificity and sensitivity are achieved.

The invention discloses shRNA inhibiting the expression of a rabbit Deptor gene, a lentivirus expression vector and a construction method and application of the lentivirus expression vector. The shRNAis pSicoR-shRNA-508 and is composed of a siRNA positive-sense strand, a loop, a siRNA antisense chain and a termination signal. shRNA can effectively and stably inhibit the expression of the rabbit Deptor gene, the influence and action mechanism of the Deptor gene on the exogenous gene expression efficiency can be studied by inhibiting the Deptor gene expression, the influence of the Deptor geneon rabbit embryonic stem cell multfunctionality maintenance and autophagy pathway related gene expression can be further verified, and a foundation is laid for stable rabbit embryonic stem cell establishment.

The invention relates to a kit for detecting a PDL-1 mRNA gene expression quantity. The kit mainly comprises specific primers and a PCR reaction reagent, and is characterized in that the sequences ofthe specific primers are as shown in the description; a PDL-1 upstream primer is as shown in the SEQ ID NO.1 and is 5'-AAGCACACGTGCCAAAGAATC-3'; and a PDL-1 downstream primer is as shown in the SEQ IDNO.2 and is 5'-TCTCGACGACGACGTTTCC-3'. The method can easily, quickly, accurately and reliably detect the gene expression quantity of the PDL-1 mRNA.

The invention discloses a method for screening and functionally analyzing related oncogenes of non-small-cell lung cancer. The method includes steps of searching mRNA [messenger RNA (ribonucleic acid)] expression chip results related to the NSCLC (non-small-cell lung cancer) from GEO (gene expression omnibus) databases http://www.ncbi.nlm.nich.gov/geo/ and acquiring mRNA expression results by the aid of GEO2R databases; searching identical mRNA gene expression results in two research results by the aid of Venn diagrams; functionally analyzing gene enrichment by the aid of bioinformatics technologies. The method has the advantages that mRNA differential expression genes are downloaded by the aid of the diversified online databases, the common differential expression genes in different research series are searched and are subjected to bioinformatics analysis, and accordingly significant exploration and bases can be provided for tumor marker screening, molecular pathogenesis and the like of the NSCLC.