55results about How to "Meet the needs of the experiment" patented technology

The invention belongs to the technical field of nano materials, and particularly relates to a graphene oxide for electrochemical reduction and a preparation method of graphene. The method comprises the following steps: dissolving graphite oxide in deionized water, and carrying out ultrasonic peeling to obtain a graphene oxide solution; cleaning electrodes and a glass container; adding the graphene oxide solution into the glass container; connecting one end of a copper conductor with cathode and anode materials, putting the electrodes into the graphene oxide solution, and respectively connecting the other end of the copper conductor with positive and negative poles of a direct-current stabilized voltage supply; switching on the direct-current stabilized voltage supply, wherein the voltage is controlled at 10-50V, and the temperature is controlled at 25-90 DEG C; and carrying out electrochemical reduction for 1-60 minutes, thereby obtaining the graphene. The graphene prepared by the method provided by the invention has the advantages of high quality; and the method is simple to operate, and has the advantages of low cost, high safety, no pollution, high-efficiency and the like.

The invention discloses an optical engine with variable view field. The optical engine comprises a cylinder body of the engine, a cylinder cover and a lengthened piston, wherein the middle part of the lengthened piston has hollowed structure, a reflector is placed in the space of the hollowed structure, and a piece of circular planar quartz glass is arranged at the top of the lengthened piston; aguide base is fixed at the upper part of the cylinder body, and cylinder sleeves are fixed between the guide base and the cylinder cover; the number of the cylinder sleeves is three, the heights of the three cylinder sleeves are consistent, and the connecting mode between each cylinder sleeve and the cylinder cover at the upper part of the cylinder sleeve and between the cylinder sleeve and the guide base at the lower part of the cylinder sleeve and the geometric dimension of the cylinder sleeves are the same to ensure interchangeability of the three cylinder sleeves; and the three cylinder sleeves are respectively a metal lengthened cylinder sleeve, an annular quartz glass cylinder sleeve and a planar mosaic glass cylinder sleeve, and variable view field of the optical engine is realizedby alternately using the three cylinder sleeves. The problem that a conventional optical engine cannot meet multiple measurement requirements at the same time is solved, and various measurement requirements are met by integrated design and adaptive design of the cylinder sleeves.

A method for preparing a PDMS chip including both square and arc-shaped channels comprises the following steps: clamping an SU-8 male mold of a cross-linked state prepared by standard photolithography with liquid PDMS to obtain a PDMS female mold with an inverted male mold pattern after the PDMS is cured; clamping the female mold PDMS with the solid uncross-linked SU-8 photoresist, heating the photoresist by a vacuum oven to make the photoresist be in a fluid state to fill the PDMS channels to obtain the uncrosslinked SU-8 male mold; selectively exposing the uncrosslinked SU-8 male mold to make the SU-8 male mold partially crosslink to form square protrusion portions; heating the male mold for some time to make the uncrosslinked portions be arc-shaped; performing a fully exposed crosslinking reaction, post-baking, solidifying the mold to get the stereotyped SU-8 square and arc-shaped male mold; and pouring with PDMS, curing, demolding to get the PDMS chip including both square and arc-shaped channels. The chip produced by the present invention is applied to preparation of a micro-fluidic chip liquid channel layer integrated with pneumatic micro-valves. The SU-8 male mold prepared by the invention is not easy to peel off from substrates. The radian of the PDMS chip arc-shaped channel is controllable. The simple and stable method is provided for the preparation of complex chips.

The invention discloses a simulation system for algal bloom emergency regulation and control under a changing environment and a method of the simulation system. The simulation system comprises a water customizing unit, a multifunctional simulation unit, an environment regulation unit, an algal bloom charging unit, a water quality monitoring unit and a control platform, wherein the water customizing unit is used for treating and customizing source water into experimental water, the multifunction simulation unit physically models according to a large-scale water area and simulates an algal bloom emergency measure, the environment regulation unit is used for emulating combined changes of field environment conditions, such as illumination, temperature and wind field, the algal bloom charging unit realizes spatial distribution simulation of algal blooms in any form at any time, the water quality monitoring unit is used for measuring spatial and temporal distribution of key water quality indexes in real time, and the control platform is used for controlling an operation state of a key device. The simulation system for the algal bloom emergency regulation and control under the changing environment and the operation method of the simulation system are suitable for prediction and optimization of emergency disposal scheme effects after large-scale water area algal bloom breaks out, and can conveniently and accurately predict implementation effects of algal bloom emergency measures, such as drainage basin water transfer, local water circulation and aeration methods.

The invention relates to a novel multi-purpose magnetic-levitation-type guide rail experiment instrument. The instrument includes levelling support legs, an experiment instrument base, arc rail frame support rods, a rotary moving shaft sliding groove at the rear of a magnetic rail, a main ruler, a micrometer ruler, a micrometer ruler sliding groove, rotation shaft support columns at the front of the magnetic rail, central lines of the rotation shaft support columns at the front of the magnetic rail, a magnetic-rail levelling pointer support frame, the magnetic rail, a rotary moving shaft at the rear of the magnetic rail, a leveling support rod at the tail end of the magnetic rail, a photogate, a pulley frame, pulley frame fixing screws and a sliding block. The lower end of the experiment instrument base is provided with the three levelling support legs, the two sides of the upper portion, corresponding to the two levelling support legs at the front and lower ends of the base respectively, of the base are provided with the rotation shaft support columns at the front of the magnetic rail, and the rotation shaft support columns at the front of the magnetic rail are used for supporting the magnetic rail. The novel multi-purpose magnetic-levitation-type guide rail experiment instrument has the advantages that a measuring cylinder moving groove is specifically formed in the base regarding the project of 'research experiments on movement law of objects in fluid, and the specific placement of a measuring cylinder needs to be determined according to the dip angle of the magnetic rail.

The invention discloses a digital experimental system based on a universal serial bus (USB) and a virtual instrument. The digital experimental system comprises a computer, a digital circuit experimental instrument module and an experimental chip, wherein the computer and the digital circuit experimental instrument module communicate through a USB port signal line and supply power to a digital circuit experimental instrument system and the experimental chip through a USB port of the computer; and a connecting line between the digital circuit experimental instrument module and the experimental chip is a standard C2 self-locking connector. By employing the digital experimental system based on the USB and the virtual instrument, a microcomputer serves as the basis, an integrated test platform is constructed, a conventional instrument is replaced, the requirements of electrician and electronic experiment teaching are met, the microcomputers can serve as computers for other computer course teaching, the equipment utilization rate is greatly improved, the resource sharing is realized, the experimental construction cost is reduced, and due to the introduction of the virtual instrument, an experimental teaching mode of combining the actual operation and the computer simulation is determined.

The invention relates to the field of material test, and in particular relates to a universal sample rod and switching device for transmission, scanning and focused ion beam electron microscopes. Thedevice comprises a sample rod, a switching sealing belt flange sleeve, a sealing flange, a corrugated pipe sleeve, and an X-Y-Z three-dimensional siding table and a support thereof; the switching sealing belt flange sleeve is fixed at the tail end of the sample rod, and a sealing ring is used between the inner wall of the switching sealing belt flange sleeve and the sample rod to achieve vacuum seal; the switching sealing belt flange sleeve is placed in the corrugated pipe sleeve, the sealing ring is used between the outer wall of the switching sealing belt flange sleeve and the inner wall ofthe corrugated pipe sleeve to achieve vacuum seal; the X-Y-Z three-dimensional siding table is fixed on the sealing flange and the corrugated pipe sleeve via the sliding table support; and thus, a sample can translate three-dimensions and rotate for 360 degrees around the axis of the rod body. According to the device provided by the invention, the processing and microstructure analysis of the sample are greatly facilitated, the work efficiency is improved, and the device is widely applicable to the various types of transmission, scanning and focused ion beam electron microscopes.

The invention discloses a structure fatigue / durability experiment system and method in a complex environment. The system comprises an environment simulation device, a loading device, a testing device, a ground anchor device and a control terminal, wherein the environment simulation device, the loading device and the testing device are all connected with the control terminal; the environment simulation device, the loading device and the testing device are all arranged on the ground anchor device; the environment simulation device comprises at least one test chamber, all the test chambers are connected in sequence, and a movable partition door is arranged between every two adjacent test chambers; each test chamber is provided with an independent environment simulation mechanism and a control mechanism, the environment simulation mechanism is connected with the control mechanism, and the control mechanism is connected with the control terminal. The environment fatigue / durability experiment of different full-scale structures and models under the coupling action of various complex environments and (random) loads can be realized, the universality is good, and the application range is wide.

The invention relates to a preparation method of a PCV2Cap protein monoclonal antibody. The preparation method comprises the steps of immunizing an animal, fusing cells, selecting positive hybridoma cells and colonizing. By adopting the preparation method, the preparation time is short, operation is simple, the valence of the extracted antibody is high, and the experimental requirement can be met.

The invention provides a heating device applied to a scanning electron microscope. The device comprises a sample crucible, a heating core ceramic, an electric heating wire and a heat radiation shielding structure, wherein the sample crucible is placed on an upper end of the heating core ceramic, the electric heating wire is wound around a surface of the heating core ceramic in a double helical manner, the heating core ceramic is heated, and the electric heating wire is made of a doped tungsten filament; the heat radiation shielding structure comprises an internal stainless steel shielding layer, a secondary external stainless steel shielding layer, an external stainless steel shielding layer, an upper stainless steel shielding layer, a secondary upper stainless steel shielding layer and a shielding base seat; inter-layer space between the internal stainless steel shielding layer, the secondary external stainless steel shielding layer and the external stainless steel shielding layer is filled with vacuum; a diameter ratio between the above three layers is determined via a heat loss equation. The heating device provided in the invention is high in heating efficiency, small in size and good in shielding effects; a problem that a conventional heating device affects normal work of the scanning electron microscope is overcome.

The invention discloses a biological centrifugal machine comprising a housing, a transmission mechanism, a drum and suspension supports. The housing is composed of an outer housing and an inner housing, the inner housing is arranged in the outer housing, a gap is reserved between the inner housing and the outer housing, the top ends of the inner housing and the outer housing are communicated through a feed inlet, a sealing cover is arranged at the feed inlet, the drum is arranged in the inner housing, the bottom end of the drum is driven by a motor outside the outer housing through the transmission mechanism, the outer wall of the inner housing and the outer housing are supported through the suspension supports, and the outer wall of the inner housing is provided with a heat insulation layer. The biological centrifugal machine has the advantages that the biological centrifugal machine is reasonable in structural design and good in separation effect by the aid of the inner housing and the outer housing, and is good in sealing performance and capable of effectively preventing escape of bacteria and viruses. Meanwhile, the biological centrifugal machine has good heat insulation effect on materials, meets experimental requirements and is safe and reliable in operation.

The invention provides application of Ad-sAxl in the establishment of a preeclampsia rat model and an establishment method of the preeclampsia rat model. The preeclampsia rat model is established by injecting Ad-sAxl into a pregnant rat through in a caudal vein manner and has the preeclampsia characteristic symptoms and symptomatic changes including elevation of blood pressure, injuries of important organs such as proteinuria, heart, liver and kidney, and the characteristic symptoms and symptomatic changes are accordant with preeclampsia symptom, signs and pathological processes of human and can reflect the systematicness and severity of diseases; and the operational controllability and the stability are good. The application can be applied to the research of occurrence and development mechanisms and control measures of preeclampsia.

The invention discloses a frequency locking system and method based on the Rydberg electromagnetic induction transparent signal. The system includes a probe light source, a coupling light source, a rubidium vapor cell, a photodetector, a sinusoidal signal source, a signal amplifier, a lock-in amplifier and a proportional-integral-integral controller; and the frequency locking method uses a coupledlight source to scan the frequency of the coupled light passing through the rubidium vapor cell to obtain a Rydberg electromagnetic induction transparent signal, and adopts an alternating magnetic field to modulate the atomic energy level to achieve the frequency modulation of the Rydberg electromagnetic induction transparent signal so as to obtain the differential signal of the Rydberg electromagnetic induction transparent signal, namely the error signals required by frequency locking, and the frequency locking for the coupled light laser is performed. The system and method provided by the invention are wide in application range, narrow in line width, simple and stable in structure, low in environmental requirement and low in cost, and are suitable for development of portable systems.

The invention discloses a method and a system for quickly switching a photomultiplier of a low-light detector. The method comprises the following steps: a gating circuit is added to a voltage divisioncircuit of the low-light detector; when the laser is turned off, a pulse low-level signal is input into the input end of the gating circuit, a potential difference is formed among the first multiplication stage, the second multiplication stage and the third multiplication stage of the voltage division circuit through the high-voltage power supply of the low-light detector, and the photomultiplierPMT is turned on; when the laser is turned on, a pulse high-level signal is input into the input end of the gating circuit, power supply of a high-voltage power supply of the low-light detector is cut off, the potential difference among the first multiplication stage, the second multiplication stage and the third multiplication stage of the voltage division circuit is zero, and the photomultiplier PMT is turned off.

The invention discloses a nanoparticle aerosol generator which comprises an insulation upper cover and a base, wherein the insulation upper cover is arranged on the base; the insulation upper cover and the base are provided with hollow cavities respectively; a metal bar is arranged in the insulation upper cover and positioned in the hollow cavity of the insulation upper cover and extends into the hollow cavity of the base; a metal needle is arranged at the free end of the metal bar; a high-voltage power supply connecting terminal is arranged at the upper end of the insulation upper cover; an air inlet and an air outlet are formed at the lower part of the base; and a metal plate is arranged on the inner surface of a bottom plate of the base. The nanoparticle aerosol generator is simple in structure, easy to operate and free of environment pollution. The metal needle and the metal plate are mounted in an insulation manner. In addition, the diameter of the generated nanoparticle is less than 100 nm. Therefore, the nanoparticle aerosol generator can more widely satisfy the requirements of the experiments of a research institution.

The invention discloses a fluorescent biosensor for simultaneously detecting two kinds of RNA as well as a preparation method and a use method thereof. When a target detection object miRNA appears in a reaction system, the capture chains cDNA1 and cDNA2 are respectively combined with the target microRNA, and the fluorescence of the CQDs and the Cy5 is recovered. Meanwhile, double-strand specific nuclease (DSN) is used to act on the double strands formed by the pDNA and the target MicroRNA, so that the microRNA participates in the next round of reaction, and the signal is amplified. The construction method provided by the invention is simple, the detection sensitivity is high, the expression quantity of two miRNAs in a sample can be quantitatively detected at the same time, the reaction condition is mild, the sensing system is stable and effective, and compared with expensive and tedious clinical gene sequencing, the method has great practical significance and development potential and is beneficial to popularization and application.

The invention discloses a novel simple apparatus for simulating gate valve wearing. An upper cover plate is connected between the upper portions of a left pressing cover and a right pressing cover, and a lower cover plate is connected between the lower portions of the left pressing cover and the right pressing cover. The upper cover plate and the lower cover plate are provided with a replaceable simulation assembly, and organic glass plates are arranged on the two sides of the central portions of the upper and lower cover plates. The replaceable simulation assembly comprises a replaceable block, a screw and a block wearing sheet, wherein the replaceable block is installed in an upper groove in the bottom surface of the upper cover plate, the upper end of the replaceable block is connected with the screw passing through the upper cover plate, the installation height of the replaceable block can be adjusted by adjusting the screw so as to simulate the opening degree of a gate valve, and the block wearing sheet is pasted to the bottom of the replaceable block. Cost is saved substantially, the opening degree of the gate valve can be determined accurately, the structure is simple, dismounting is very convenient, and workload can be reduced substantially. Particle movement trace can be observed through the organic glass plate by using a high-speed photographic instrument, and the novel simple apparatus is widely applicable.

The invention provides a method for efficiently synthesizing long-fragment and complex-fragment DNA in vitro. Firstly, primary fragments are synthesized by a primer; secondly, fragments are assembled; finally, the fragments are assembled to the DNA sequence. The DNA fragments synthesized by the method has stable synthetic quality and are not interfered by DNA complexity and GC content; superlong DNA fragments can be synthesized; the DNA fragments with length being within 30 kb can be stably synthesized; the requirements of the modern molecular experiments are met.

The invention discloses a laboratory intelligent control integrated tower crane, comprising a frame, wherein upright posts are connected to four corners of a top end of the frame by screws, reinforcing ribs are connected to left and right sides of an inner cavity of the frame by screws along a front and back direction, slide rails are connected to the left and right sides of the top end of the frame by screws along the front and back direction, slide blocks capable of sliding front and back are inserted into central positions of the inner cavities of the slide rails, cross beams are connectedto front and back sides of the slide blocks by screws, a gear rack is connected to a top end of the reinforcing rib located on the left side, a motor is connected to a left end of an inner side of each cross beam by screws, an output end of the motor is in key connection with a gear engaged with the gear rack. The laboratory intelligent control integrated tower crane can drive an integrated box tomove according to the experimental position, so that the use is more flexible, the installation of experimental equipment is time-saving and labor-saving, the experiment time is shortened, the experiment efficiency and quality are improved, and the experiment requirements are greatly satisfied.

The invention discloses an in-vitro expression method of pig Six1 protein and a preparation method of polyclonal antibodies of the pig Six1 protein. The preparation method comprises the following steps: cloning a pig Six1 gene through primer design; constructing an escherichia coli recombinant expression vector pET-30a(+)-pSix1; transferring the constructed recombinant expression vector pET-30a(+)-pSix1 into the escherichia coli BL21(DE3) to construct and express a recombinant strain of the pig Sixl protein; optimizing the induction expression conditions of the recombinant strain and purifying the recombinant pig Six1 protein; and preparing the pig Six1 polyclonal antibodies according to a conventional polyclonal antibody preparation method with the purified recombinant pig Six1 protein as the antigen. According to the method disclosed by the invention, the in vitro expression of the pig Six1 protein is achieved and the pig Sixl polyclonal antibodies with high tier and good specificity are prepared, and therefore the requirements of relevant experiments are met.

The invention is applicable to the technical field of fertilizer efficiency verification and provides a verification device and method for fertilizer efficiency of fertilizer. The verification device for the fertilizer efficiency of the fertilizer comprises a planting container and a cultivating carrier for planting plants, wherein the width size of a top opening of the planting container is 10cm to 11cm, the width size of the bottom is 7cm to 8cm and the height is 10cm to 11cm; the cultivating carrier is arranged in the planting container and is used for planting testing plants for verifying the fertilizer efficiency. The verification method comprises a verification device selection step, an experiment arrangement step, an experiment data collection step and a data analysis step. According to the verification device and method for the fertilizer efficiency of the fertilizer, provided by the invention, the verification device is reasonable in design size and low in cost; wastes of resources are avoided; the verification method is simple and easy to operate and convenient to manage; the fertilizer efficiency verification duration of the fertilizer can be shortened and the improvement of a development progress of new products is facilitated.

The invention discloses a glass plate for manufacturing of protein gel. The glass plate comprises a large glass plate, a small glass plate and a comb, wherein the comb is suitable for being inserted between the large glass plate and the small glass plate in the gelling process; a plurality of wedge-shaped glass strips are arranged at the upper part of the large glass plate at intervals along the inserting direction of the comb, and the wedge-shaped glass strips are suitable for extending into gaps between comb teeth of the comb when the comb is inserted. The glass plate has the advantage thatthe wedge-shaped glass strips on the large glass plate correspond to the gaps between the adjacent comb teeth of the comb, so as to effectively avoid the situation that the gel cannot be used normallydue to occurrence of air bubbles in the protein gel sampling hole adhesive strip or breakage in the comb inserting process.

The invention discloses a multi-gene expression and silencing system controlled by a heat shock protein gene promoter and a tetracycline gene promoter. The heat shock protein gene promoter HSPP and the tetracycline gene promoter TETp in the same plasmid system do not influence each other and independently control expression of downstream target genes or shRNAs (small hairpin ribonucleic acids) respectively, expression of single gene or shRNA can be controlled manually, and co-expression of two genes or shRNAs can be also controlled manually. The multi-gene expression and silencing system provides conditions for multi-gene specificity coordination control, solves the problem of low expression efficiency caused by gene co-expression implemented with a multi-plasmid cotransfection method at present, adopts a lentivirus carrying system to realize efficient expression of selective genes or shRNAs in cells having difficulty in transfection, meets various experimental requirements, provides a multi-gene target control platform, and provides more possibilities for gene therapy.

The invention discloses preparation of a multifunctional photo-thermal diagnosis and treatment agent based on a 2D Ti3C2MXene nanosheet and application of the multifunctional photo-thermal diagnosis and treatment agent to preparation of a photo-thermal medicine for tumor treatment, and belongs to the technical field of medicine. The multifunctional photo-thermal diagnosis and treatment agent is a Ti3C2 nanosheet loaded with NaErF4: 0.5% Tm and NaLuF4 nano particles of a core-shell structure, and the surface of the photosensitizer Ti3C2 nanosheet is modified with the NaErF4: 0.5% Tm and NaLuF4 nano particles subjected to surface modification to obtain the multifunctional photo-thermal diagnosis and treatment agent. The multifunctional photo-thermal diagnosis and treatment agent has high photo-thermal conversion efficiency (eta = 43.62%) and good photo-thermal stability and biocompatibility, and growth inhibition and ablation of tumors can be effectively completed; meanwhile, efficient MR / NIR-IIb multi-mode imaging can be guided, the treatment process can be guided and monitored, and a diagnosis and treatment agent can be better applied to clinical medicine treatment.

The invention discloses a perfluoropropane lipid microsphere for injection for angiography of cardiovascular diseases. The preparation method comprises the following steps of: weighing a certain amount of DPPC, DPPA, span 60, Tween 80 and a small amount of PBS, fully mixing and grinding, diluting PBS to 250ml, acting by an ultrasonic dispersion instrument for 30min, introducing nitrogen for 2min,sealing the system, filling perfluoropropane gas into the system for 2 minutes (the flow is 1ml / s), and performing high-shear dispersion at different speeds and action time when a dispersion tool bitextends into the liquid level to obtain a white uniform emulsion. The invention belongs to the technical field of radiography. OFP gas contained in the perfluoropropane human serum albumin microsphereinjection is completely eliminated from the lung in a prototype, and is not accumulated in vivo or metabolized in vivo; 2, the OFP is detected by adopting a GC-MS method, so that the method has the characteristics of accuracy, sensitivity and precision, and can fully meet the requirements of experiments; and 3, the medicine is safe and good in tolerance under the condition of reagent dosage.