55results about How to "Meet the needs of the experiment" patented technology

The invention belongs to the technical field of nano materials, and particularly relates to a graphene oxide for electrochemical reduction and a preparation method of graphene. The method comprises the following steps: dissolving graphite oxide in deionized water, and carrying out ultrasonic peeling to obtain a graphene oxide solution; cleaning electrodes and a glass container; adding the graphene oxide solution into the glass container; connecting one end of a copper conductor with cathode and anode materials, putting the electrodes into the graphene oxide solution, and respectively connecting the other end of the copper conductor with positive and negative poles of a direct-current stabilized voltage supply; switching on the direct-current stabilized voltage supply, wherein the voltage is controlled at 10-50V, and the temperature is controlled at 25-90 DEG C; and carrying out electrochemical reduction for 1-60 minutes, thereby obtaining the graphene. The graphene prepared by the method provided by the invention has the advantages of high quality; and the method is simple to operate, and has the advantages of low cost, high safety, no pollution, high-efficiency and the like.

A method for preparing a PDMS chip including both square and arc-shaped channels comprises the following steps: clamping an SU-8 male mold of a cross-linked state prepared by standard photolithography with liquid PDMS to obtain a PDMS female mold with an inverted male mold pattern after the PDMS is cured; clamping the female mold PDMS with the solid uncross-linked SU-8 photoresist, heating the photoresist by a vacuum oven to make the photoresist be in a fluid state to fill the PDMS channels to obtain the uncrosslinked SU-8 male mold; selectively exposing the uncrosslinked SU-8 male mold to make the SU-8 male mold partially crosslink to form square protrusion portions; heating the male mold for some time to make the uncrosslinked portions be arc-shaped; performing a fully exposed crosslinking reaction, post-baking, solidifying the mold to get the stereotyped SU-8 square and arc-shaped male mold; and pouring with PDMS, curing, demolding to get the PDMS chip including both square and arc-shaped channels. The chip produced by the present invention is applied to preparation of a micro-fluidic chip liquid channel layer integrated with pneumatic micro-valves. The SU-8 male mold prepared by the invention is not easy to peel off from substrates. The radian of the PDMS chip arc-shaped channel is controllable. The simple and stable method is provided for the preparation of complex chips.

The invention provides a flow measuring device and method. The device comprises a discharge pipe used for introducing a water flow under test; a header tank used for acquiring the water flow introduced by the discharge pipe; a measurement part used for measuring the weight of the header tank and then transmitting the weight; and a data processing device used for receiving the weight and calculating the rate of the water flow based on the weight. The device and the method have the advantages of high precision of measurement data, wide flow application range, high data time resolution, small data fluctuation and the like. Therefore, the device and the method are especially suitable for the small-flow continuous runoff measurement.

The invention discloses a simulation system for algal bloom emergency regulation and control under a changing environment and a method of the simulation system. The simulation system comprises a water customizing unit, a multifunctional simulation unit, an environment regulation unit, an algal bloom charging unit, a water quality monitoring unit and a control platform, wherein the water customizing unit is used for treating and customizing source water into experimental water, the multifunction simulation unit physically models according to a large-scale water area and simulates an algal bloom emergency measure, the environment regulation unit is used for emulating combined changes of field environment conditions, such as illumination, temperature and wind field, the algal bloom charging unit realizes spatial distribution simulation of algal blooms in any form at any time, the water quality monitoring unit is used for measuring spatial and temporal distribution of key water quality indexes in real time, and the control platform is used for controlling an operation state of a key device. The simulation system for the algal bloom emergency regulation and control under the changing environment and the operation method of the simulation system are suitable for prediction and optimization of emergency disposal scheme effects after large-scale water area algal bloom breaks out, and can conveniently and accurately predict implementation effects of algal bloom emergency measures, such as drainage basin water transfer, local water circulation and aeration methods.

The invention relates to the field of material test, and in particular relates to a universal sample rod and switching device for transmission, scanning and focused ion beam electron microscopes. Thedevice comprises a sample rod, a switching sealing belt flange sleeve, a sealing flange, a corrugated pipe sleeve, and an X-Y-Z three-dimensional siding table and a support thereof; the switching sealing belt flange sleeve is fixed at the tail end of the sample rod, and a sealing ring is used between the inner wall of the switching sealing belt flange sleeve and the sample rod to achieve vacuum seal; the switching sealing belt flange sleeve is placed in the corrugated pipe sleeve, the sealing ring is used between the outer wall of the switching sealing belt flange sleeve and the inner wall ofthe corrugated pipe sleeve to achieve vacuum seal; the X-Y-Z three-dimensional siding table is fixed on the sealing flange and the corrugated pipe sleeve via the sliding table support; and thus, a sample can translate three-dimensions and rotate for 360 degrees around the axis of the rod body. According to the device provided by the invention, the processing and microstructure analysis of the sample are greatly facilitated, the work efficiency is improved, and the device is widely applicable to the various types of transmission, scanning and focused ion beam electron microscopes.

The invention provides a heating device applied to a scanning electron microscope. The device comprises a sample crucible, a heating core ceramic, an electric heating wire and a heat radiation shielding structure, wherein the sample crucible is placed on an upper end of the heating core ceramic, the electric heating wire is wound around a surface of the heating core ceramic in a double helical manner, the heating core ceramic is heated, and the electric heating wire is made of a doped tungsten filament; the heat radiation shielding structure comprises an internal stainless steel shielding layer, a secondary external stainless steel shielding layer, an external stainless steel shielding layer, an upper stainless steel shielding layer, a secondary upper stainless steel shielding layer and a shielding base seat; inter-layer space between the internal stainless steel shielding layer, the secondary external stainless steel shielding layer and the external stainless steel shielding layer is filled with vacuum; a diameter ratio between the above three layers is determined via a heat loss equation. The heating device provided in the invention is high in heating efficiency, small in size and good in shielding effects; a problem that a conventional heating device affects normal work of the scanning electron microscope is overcome.

The invention provides application of Ad-sAxl in the establishment of a preeclampsia rat model and an establishment method of the preeclampsia rat model. The preeclampsia rat model is established by injecting Ad-sAxl into a pregnant rat through in a caudal vein manner and has the preeclampsia characteristic symptoms and symptomatic changes including elevation of blood pressure, injuries of important organs such as proteinuria, heart, liver and kidney, and the characteristic symptoms and symptomatic changes are accordant with preeclampsia symptom, signs and pathological processes of human and can reflect the systematicness and severity of diseases; and the operational controllability and the stability are good. The application can be applied to the research of occurrence and development mechanisms and control measures of preeclampsia.

The invention discloses a frequency locking system and method based on the Rydberg electromagnetic induction transparent signal. The system includes a probe light source, a coupling light source, a rubidium vapor cell, a photodetector, a sinusoidal signal source, a signal amplifier, a lock-in amplifier and a proportional-integral-integral controller; and the frequency locking method uses a coupledlight source to scan the frequency of the coupled light passing through the rubidium vapor cell to obtain a Rydberg electromagnetic induction transparent signal, and adopts an alternating magnetic field to modulate the atomic energy level to achieve the frequency modulation of the Rydberg electromagnetic induction transparent signal so as to obtain the differential signal of the Rydberg electromagnetic induction transparent signal, namely the error signals required by frequency locking, and the frequency locking for the coupled light laser is performed. The system and method provided by the invention are wide in application range, narrow in line width, simple and stable in structure, low in environmental requirement and low in cost, and are suitable for development of portable systems.

The invention discloses a method and a system for quickly switching a photomultiplier of a low-light detector. The method comprises the following steps: a gating circuit is added to a voltage divisioncircuit of the low-light detector; when the laser is turned off, a pulse low-level signal is input into the input end of the gating circuit, a potential difference is formed among the first multiplication stage, the second multiplication stage and the third multiplication stage of the voltage division circuit through the high-voltage power supply of the low-light detector, and the photomultiplierPMT is turned on; when the laser is turned on, a pulse high-level signal is input into the input end of the gating circuit, power supply of a high-voltage power supply of the low-light detector is cut off, the potential difference among the first multiplication stage, the second multiplication stage and the third multiplication stage of the voltage division circuit is zero, and the photomultiplier PMT is turned off.

The invention discloses a nanoparticle aerosol generator which comprises an insulation upper cover and a base, wherein the insulation upper cover is arranged on the base; the insulation upper cover and the base are provided with hollow cavities respectively; a metal bar is arranged in the insulation upper cover and positioned in the hollow cavity of the insulation upper cover and extends into the hollow cavity of the base; a metal needle is arranged at the free end of the metal bar; a high-voltage power supply connecting terminal is arranged at the upper end of the insulation upper cover; an air inlet and an air outlet are formed at the lower part of the base; and a metal plate is arranged on the inner surface of a bottom plate of the base. The nanoparticle aerosol generator is simple in structure, easy to operate and free of environment pollution. The metal needle and the metal plate are mounted in an insulation manner. In addition, the diameter of the generated nanoparticle is less than 100 nm. Therefore, the nanoparticle aerosol generator can more widely satisfy the requirements of the experiments of a research institution.

The invention discloses a novel simple apparatus for simulating gate valve wearing. An upper cover plate is connected between the upper portions of a left pressing cover and a right pressing cover, and a lower cover plate is connected between the lower portions of the left pressing cover and the right pressing cover. The upper cover plate and the lower cover plate are provided with a replaceable simulation assembly, and organic glass plates are arranged on the two sides of the central portions of the upper and lower cover plates. The replaceable simulation assembly comprises a replaceable block, a screw and a block wearing sheet, wherein the replaceable block is installed in an upper groove in the bottom surface of the upper cover plate, the upper end of the replaceable block is connected with the screw passing through the upper cover plate, the installation height of the replaceable block can be adjusted by adjusting the screw so as to simulate the opening degree of a gate valve, and the block wearing sheet is pasted to the bottom of the replaceable block. Cost is saved substantially, the opening degree of the gate valve can be determined accurately, the structure is simple, dismounting is very convenient, and workload can be reduced substantially. Particle movement trace can be observed through the organic glass plate by using a high-speed photographic instrument, and the novel simple apparatus is widely applicable.

The invention discloses a laboratory table for a physical experiment. The laboratory table comprises a working cabinet, wherein an opening/closing door is arranged at the lower part of the working cabinet; the upper part of the working cabinet is in sliding connection with a drawer through a sliding rail; an experiment platform is fixedly connected with the top of the working cabinet; a table legis fixedly connected with one side, far away from the working cabinet, of the bottom of the experiment platform; a supporting frame is fixedly connected between the table leg and the working cabinet;a rotary working box is fixedly connected with the top of the supporting frame; a rotary motor is fixedly connected with the middle position of the bottom of an inner wall of the rotary working box; the top of an output shaft of the rotary motor is fixedly connected with a three-claw chuck through a safety device; the laboratory table relates to the technical field of the physical experiment. By adopting the laboratory table for the physical experiment, the aim of carrying out the physical experiment is realized; various physical experiments can be carried out and more physical experiments aresupported; different experiment platforms and experiment fields do not need to be replaced, the experiment efficiency and speed can be improved and the experiment safety is ensured.

The invention provides a method for efficiently synthesizing long-fragment and complex-fragment DNA in vitro. Firstly, primary fragments are synthesized by a primer; secondly, fragments are assembled; finally, the fragments are assembled to the DNA sequence. The DNA fragments synthesized by the method has stable synthetic quality and are not interfered by DNA complexity and GC content; superlong DNA fragments can be synthesized; the DNA fragments with length being within 30 kb can be stably synthesized; the requirements of the modern molecular experiments are met.

The invention discloses a laboratory intelligent control integrated tower crane, comprising a frame, wherein upright posts are connected to four corners of a top end of the frame by screws, reinforcing ribs are connected to left and right sides of an inner cavity of the frame by screws along a front and back direction, slide rails are connected to the left and right sides of the top end of the frame by screws along the front and back direction, slide blocks capable of sliding front and back are inserted into central positions of the inner cavities of the slide rails, cross beams are connectedto front and back sides of the slide blocks by screws, a gear rack is connected to a top end of the reinforcing rib located on the left side, a motor is connected to a left end of an inner side of each cross beam by screws, an output end of the motor is in key connection with a gear engaged with the gear rack. The laboratory intelligent control integrated tower crane can drive an integrated box tomove according to the experimental position, so that the use is more flexible, the installation of experimental equipment is time-saving and labor-saving, the experiment time is shortened, the experiment efficiency and quality are improved, and the experiment requirements are greatly satisfied.

The invention discloses an in-vitro expression method of pig Six1 protein and a preparation method of polyclonal antibodies of the pig Six1 protein. The preparation method comprises the following steps: cloning a pig Six1 gene through primer design; constructing an escherichia coli recombinant expression vector pET-30a(+)-pSix1; transferring the constructed recombinant expression vector pET-30a(+)-pSix1 into the escherichia coli BL21(DE3) to construct and express a recombinant strain of the pig Sixl protein; optimizing the induction expression conditions of the recombinant strain and purifying the recombinant pig Six1 protein; and preparing the pig Six1 polyclonal antibodies according to a conventional polyclonal antibody preparation method with the purified recombinant pig Six1 protein as the antigen. According to the method disclosed by the invention, the in vitro expression of the pig Six1 protein is achieved and the pig Sixl polyclonal antibodies with high tier and good specificity are prepared, and therefore the requirements of relevant experiments are met.

The invention is applicable to the technical field of fertilizer efficiency verification and provides a verification device and method for fertilizer efficiency of fertilizer. The verification device for the fertilizer efficiency of the fertilizer comprises a planting container and a cultivating carrier for planting plants, wherein the width size of a top opening of the planting container is 10cm to 11cm, the width size of the bottom is 7cm to 8cm and the height is 10cm to 11cm; the cultivating carrier is arranged in the planting container and is used for planting testing plants for verifying the fertilizer efficiency. The verification method comprises a verification device selection step, an experiment arrangement step, an experiment data collection step and a data analysis step. According to the verification device and method for the fertilizer efficiency of the fertilizer, provided by the invention, the verification device is reasonable in design size and low in cost; wastes of resources are avoided; the verification method is simple and easy to operate and convenient to manage; the fertilizer efficiency verification duration of the fertilizer can be shortened and the improvement of a development progress of new products is facilitated.

The invention discloses preparation of a multifunctional photo-thermal diagnosis and treatment agent based on a 2D Ti3C2MXene nanosheet and application of the multifunctional photo-thermal diagnosis and treatment agent to preparation of a photo-thermal medicine for tumor treatment, and belongs to the technical field of medicine. The multifunctional photo-thermal diagnosis and treatment agent is a Ti3C2 nanosheet loaded with NaErF4: 0.5% Tm and NaLuF4 nano particles of a core-shell structure, and the surface of the photosensitizer Ti3C2 nanosheet is modified with the NaErF4: 0.5% Tm and NaLuF4 nano particles subjected to surface modification to obtain the multifunctional photo-thermal diagnosis and treatment agent. The multifunctional photo-thermal diagnosis and treatment agent has high photo-thermal conversion efficiency (eta = 43.62%) and good photo-thermal stability and biocompatibility, and growth inhibition and ablation of tumors can be effectively completed; meanwhile, efficient MR/NIR-IIb multi-mode imaging can be guided, the treatment process can be guided and monitored, and a diagnosis and treatment agent can be better applied to clinical medicine treatment.