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IgA nephropathy diagnosis marker combination and application thereof

A nephropathy and diagnostic kit technology, applied in the field of nephropathy detection, can solve the problems such as the inability of patients to obtain a clear diagnosis, the inability to perform early diagnosis by renal puncture, and the inability to independently apply the clinical diagnosis of IgA nephropathy.

Pending Publication Date: 2021-11-16
SHENZHEN LUWEI BIOTECHNOLOGY (BIOMANIFOLD TECH CO) LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the gold standard for the diagnosis of IgA nephropathy is pathological tissue biopsy of renal puncture. However, invasive renal puncture has several disadvantages: (1) Renal puncture cannot be used for early diagnosis and can only detect patients with established renal damage
(2) Renal puncture is risky, because many patients have relative contraindications for renal puncture, or the hospital does not have the conditions for pathological diagnosis of renal puncture, so patients cannot obtain a clear diagnosis and receive targeted treatment
(3) The medical cost of renal puncture is high, which is equivalent to one operation and needs to be hospitalized for one week
[0005] However, in the above studies, only single gene diagnostic markers are often screened, and these genes are usually involved in multiple biological processes, which are not enough to be independently used in the clinical diagnosis of IgA nephropathy. Therefore, it is necessary to provide a marker with good diagnostic value combination

Method used

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  • IgA nephropathy diagnosis marker combination and application thereof
  • IgA nephropathy diagnosis marker combination and application thereof
  • IgA nephropathy diagnosis marker combination and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0175] This embodiment provides a method for screening IgA nephropathy gene diagnostic markers using mRNA gene expression data, the process is as follows:

[0176] 1. Data set preparation

[0177] 1. Download the dataset GSE93798 from the Gene Expression Omnibus (GEO). GSE93798 is gene chip data (Affymetrix GPL22945 platform, Affyme-trix human genome U133 Plus 2.0 array), which contains 22 cases of healthy people and 20 cases of IgA nephropathy patients kidney tissue samples, more than 20,000 gene probes.

[0178] 2. After excluding gene transcripts with extremely low expression (the number of samples with non-zero expression does not exceed 10), the number of remaining genes is 19764.

[0179] 3. Data standardization: For each sample, calculate the median of all gene expression levels. The normalized expression of each sample is: original expression level - median of all gene expression levels in the sample, and the standardization removes the input amount of sample mRNA di...

Embodiment 2

[0210] This embodiment provides a device for assessing the risk of IgA nephropathy. The device includes a processor and a memory, and the memory stores a computer program that can be executed by the processor. The method of using this device to assess the risk of IgA nephropathy for subjects is as follows:

[0211] 1. Select the subjects' peripheral blood samples to extract exosomal mRNA.

[0212]2. Send the extracted mRNA into a detection device (for example, a standard qPCR platform) to perform quantitative data on the expression of 9 gene diagnostic markers provided in Example 1: ADIPOR2, CDK14, CYB561D1, DOCK10, IL33, PCDH18, PLEKHG2, RASGRF2, VIL1.

[0213] 3. Use the device to retrain a linear regression model using clinical observations (e.g., proteinuria, eGFR, pathological grade of renal biopsy, 5- or 10-year risk of uremia, drug effectiveness prediction, drug resistance) as target variables , determine the parameter vector w for the peripheral blood sample n (n=0~...

Embodiment 3

[0215] This embodiment provides a kit, including reagents capable of quantifying the mRNA levels of ADIPOR2, CDK14, CYB561D1, DOCK10, IL33, PCDH18, PLEKHG2, RASGRF2, VIL1, the reagents include reverse transcriptase, primers, Taq enzymes, fluorescent dyes, etc. .

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Abstract

The invention discloses an IgA nephropathy diagnosis marker combination and application thereof. In the first aspect, the application provides: a quantitative detection gene ADIPOR2; CDK14; at least one of CYB561D1 and CYB561D2; DOCK10; IL33; at least one of PCDH18 and PCDH17; at least one of PLEKHG2 and PLEKHG3; and at least one of RASDRF2 and RASGRF1. The invention discloses application of a reagent of VIL1 in preparation of a diagnostic kit for IgA nephropathy. According to the application, a combination of the nine genes is screened from total mRNA gene expression group data, wherein whether a subject suffers from the IgA nephropathy or not can be efficiently and accurately diagnosed by performing quantitative detection on the subject based on the nine genes.

Description

technical field [0001] The present application relates to the technical field of nephropathy detection, in particular to IgA nephropathy diagnostic marker combination and its application. Background technique [0002] Immunoglobulin (IgA) nephropathy is the most common primary glomerular disease. More than 30% of patients progress to end-stage renal disease (ESRD) 10-20 years after onset, making IgA nephropathy one of the most common causes of uremia. Currently, the gold standard for the diagnosis of IgA nephropathy is pathological tissue biopsy of renal biopsy. However, invasive renal biopsy has several disadvantages: (1) Renal biopsy cannot be used for early diagnosis and can only detect patients with established renal damage. (2) Renal puncture is risky, because many patients have relative contraindications for renal puncture, or the hospital does not have the conditions for pathological diagnosis of renal puncture, so patients cannot obtain a clear diagnosis and receive...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883G01N33/6893C12Q2600/158G01N2800/347G01N2800/60
Inventor 饶皑炳
Owner SHENZHEN LUWEI BIOTECHNOLOGY (BIOMANIFOLD TECH CO) LTD
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