40 results about "Glutamine synthetase gene" patented technology

The invention discloses LAMP (loop-mediated isothermal amplification) primer composition for detecting colletotrichum gloeosporioides and an application of the LAMP primer composition. A GS (glutamine synthetase) gene is used as a target gene, primers which have high specificity and high sensitivity and can be used for LAMP detection of the colletotrichum gloeosporioides are designed and screened out, and the LAMP primer composition consists of a forward outer primer GS-F3 shown in SEQ ID NO.2, a reverse outer primer GS-B3 shown in SEQ ID NO.3, a forward inner primer GS-FIP shown in SEQ ID NO.4, a reverse inner primer GS-BIP shown in SEQ ID NO.5, a loop primer GS-LF shown in SEQ ID NO.6 and a loop primer GS-LB shown in SEQ ID NO.7. The primer composition is mainly used for quickly detecting the colletotrichum gloeosporioides, and only 2 hours are spent on detection each time.

The invention relates to a special plant expression vector pH2-35S-PrbcS-GS1 which comprises an arabidopsis thaliana cytoplasm glutamine synthetase gene GS1 and can improve the utilization rate of a plant nitrogen element. A method of RT-PCR is used for cloning the GS1 gene from the arabidopsis thaliana of a model plant, a photoinduction type promotor (the promotor of a a small subunit Rubisco) is used for controlling the excessive expression of the GS1 gene in a plant leaf and a leaf disc conversion method is used for transferring the GS1 gene into a pPZP221-PrbcS-Dof1 type transgene tobacco. An experiment result shows that the GS1 gene can be normally transferred in the transgene tobacco; under the nutrition condition of low nitrogen and the growing conditions of indoor irradiation for 24 hours of 2000LUX and 25 DEG C, the growing situation of the plant transferred with the single gene of Dof1 is (the expression of the gene is controlled by the photoinduction type promotor Prbcs) is a little better than that of a contrast tobacco (a wild type without transgene); after being transferred under the natural growing condition of a green house, the growing situation of the tobacco which is simultaneously transferred with the GS1 gene and the Dof1 gene shows remarkable growing advantages than that of the contrast plant; and therefore, simultaneously and excessively expressing the GS1 gene and the Dof1 gene, can improve the efficiency of the GS / GOGAT (glutamine synthetase / glutamic acid synthetase) approaches in the leaf more extensively, thereby improving the utilization rate of the plant nitrogen element. The vector can be broadly applied to the molecule breeding of crops, improving the utilization rate of the plant nitrogen element thereof and the durability to the nutrition condition of low nitrogen and being capable of obtaining a higher yield under the conditions of applying less fertilizers and even not applying the fertilizers.

The invention provides high-nucleoside-yield bacillus subtilis engineering bacterium as well as a construction method and application thereof The invention also provides a method for producing nucleoside by fermentation. The method comprises the following steps of (1) enhancing a glutamate synthase gene gltA for encoding an NCBI reference sequence WP-009967365.1 on a bacillus subtilis chromosome;and / or enhancing a glutamine synthetase gene glnA for encoding an NCBI reference sequence WP-003231737.1 on the chromosome of the bacillus subtilis; and (2) applying the gene-enhanced strain obtainedin the step (1) to fermentation production of nucleoside. The bacillus subtilis engineering bacterium provided by the invention is a high-nucleoside-yield strain, can effectively accumulate nucleoside, improves the yield of nucleoside, and lays a foundation for industrial production of nucleoside.

The invention discloses a glutamine synthetase and its special-purpose expression project bacterium and its allocation, which is to provide a glutamine synthetase and its special-purpose expression project bacterium and its allocation. The said glutamine synthetase is prepared by abrupt change of the 405th amino acid residue of amino end to phenylalanine corynebacterium glutamicum. The engineering bacteria prepare the recombination bacteria by importing the recombination expression carrier containing the said glutamine synthetase gene into host bacteria. The glutamine synthetase produced by the engineering bacteria can specially act on NH4+ and aminoglutaric acid, the durability to the high concentration ammonium ion has been distinctively improved because of the dissolution of adenosine acylation modification, the major bottleneck of glutamine with enzymatical production has been solved, and the concentration of the obtained glutamine can reach 47.6g / L. The invention has higher practicability and flackery and has wide application foreground in glutamine commercial manufacture.

The invention discloses a mutant site of a glufosinate-ammonium resisting Eleusine indica species. The mutant site is located at a 175th position of a nucleotide sequence shown in SEQ ID NO: 1 of a target enzyme, i.e., glutamine synthetase gene EiGS1-S cloned from glufosinate-ammonium sensitive Eleusine indica, and generated mutation is A-G. The invention further discloses a primer dCAPs for detecting a gene mutation site of the glufosinate-ammonium resisting Eleusine indica species, a detection method for the glufosinate-ammonium resisting Eleusine indica species and application of the primerin preparation of a kit or biological preparation for detecting the glufosinate-ammonium resisting Eleusine indica species.

The invention relates to a glutamine synthetase gene, a protein coded by same and a cloning method thereof. The gene is from dunaliella, the base sequence of the gene is represented by SEQ ID No.1, and the gene has a function of synthesizing glutamine synthetase and is proved to be capable of obviously improve the glutamine synthesizing capacity of Escherichia coli which is a model organism. Thus, it is indicated that the disclosed full-length gene and the amino acid sequence of the gene have application values in plant gene engineering aspects of improving nitrogen utilization rate of organisms and increasing nitrogen content in organisms.

The invention discloses a glutamine synthetase and its special-purpose expression project bacterium and its allocation, which is to provide a glutamine synthetase and its special-purpose expression project bacterium and its allocation. The said glutamine synthetase is prepared by abrupt change of the 405th amino acid residue of amino end to phenylalanine corynebacterium glutamicum. The engineering bacteria prepare the recombination bacteria by importing the recombination expression carrier containing the said glutamine synthetase gene into host bacteria. The glutamine synthetase produced by the engineering bacteria can specially act on NH4+ and aminoglutaric acid, the durability to the high concentration ammonium ion has been distinctively improved because of the dissolution of adenosine acylation modification, the major bottleneck of glutamine with enzymatical production has been solved, and the concentration of the obtained glutamine can reach 47.6g / L. The invention has higher practicability and flackery and has wide application foreground in glutamine commercial manufacture.

The invention relates to a glutamine synthetase gene of acinetobacter as well as encoding protein and a cloning method of the glutamine synthetase gene. The gene is from acinetobacter bacteria and adopts a base sequence represented by SEQ ID NO.1. The gene encodes glutamine synthetase, can play functions of glutamine synthetase in escherichia coli and can recover glutamine synthesis capacity of escherichia coli mutants, and application values of gene in efficient utilization of other biological nitrogen are further predicted simultaneously.

Disclosed herein are methods and compositions for inactivating the glutamine synthetase (GS) gene using a fusion protein comprising a zinc finger protein and a cleavage domain or half-domain. Also provided is a polynucleotide encoding the fusion protein, a cell comprising the polynucleotide and the fusion protein.

The present disclosure is directed to genetically engineered bacteria strains with enhanced biofuel-producing capabilities from cellulosic substrates. The bacteria strains of the present disclosure comprise an inactivated Type I glutamine synthetase gene. The present disclosure is also directed to methods of producing biofuels from cellulosic biomass using the genetically engineered bacteria strains.

The invention discloses cloning and application of a poa pratensis glutamine synthetase gene PpGS1.1 promoter, relates to the field of gene engineering, and aims to fill the blank of research on a poa pratensis PpGS1.1 promoter sequence in existing resources and lay a foundation for improving poa pratensis nitrogen absorption and utilization capacity and improving the turf use quality of poa pratensis. According to the primer disclosed by the invention, the specific primer is SP1: 5 '-GCGGCCATGACAAGAATAAGAAG-3'; sP2: 5 '-GCGATGATCTTCTTCGGTGGTGTT-3', and SP3: 5 '- SP3: 5 '-AGATCGGTGAGGAGCGCCCATAA-3', and SP3: 5 '- And the SP4 is 5 '-TCGAACCGATGTACCGTATATGG-3', and the SP4 is 5 '- The nucleotide sequence of the promoter fragment of the PpGS1.1 gene is as shown in SEQ ID NO. 1.

The present invention provides a method for integrating multiple exogenous genes at specified sites in the host cell genome. The present invention also provides a method for modifying the host cell genome, the method comprising: a. integrating an exogenous gene at a designated site in the host cell genome; and / or b. knocking out fucose transfer in the host cell genome Enzyme 8 gene and / or glutamine synthase gene and / or α2,3-sialyltransferase gene 4 and / or α2,3-sialyltransferase gene 6. The present invention also provides host cells prepared according to the methods of the present invention. The present invention also provides the use of the host cell for producing the target protein. The cell genome of the CHO strain provided by the present invention integrates genes capable of improving protein expression at a specific point, which is of great significance for the development of novel antibodies and high-end imitation antibodies.