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Cloning and application of Poa pratensis glutamine synthetase gene PpGS1.1 promoter

A technology of glutamine and bluegrass, applied in the field of biotechnology breeding, to improve nitrogen absorption and utilization, and enhance transcriptional regulation

Active Publication Date: 2022-07-12
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the invention patent is to clone the cDNA sequence of the PpGS1 gene, and does not involve the promoter of the PpGS1.1 gene

Method used

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  • Cloning and application of Poa pratensis glutamine synthetase gene PpGS1.1 promoter
  • Cloning and application of Poa pratensis glutamine synthetase gene PpGS1.1 promoter
  • Cloning and application of Poa pratensis glutamine synthetase gene PpGS1.1 promoter

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Experimental program
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specific Embodiment approach 1

[0040]Embodiment 1: a kind of primer of the present embodiment for cloning the promoter fragment of the bluegrass glutamine synthase PpGS1.1 gene, the primer sequence is as follows:

[0041] SP1: 5'-GCGGCCATGACAAGAATAAGAAG-3';

[0042] SP2: 5'-GCGATGATCTTCTCGGTGGTGT-3';

[0043] SP3: 5'-AGATCGTGAGGAGCGCCATAA-3';

[0044] SP4: 5'-TCGAACCGATGTACCGTATATGG-3'.

specific Embodiment approach 2

[0045] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the nucleotide sequence of the promoter fragment of the PpGS1.1 gene is shown in SEQ ID NO.1. Others are the same as the first embodiment.

specific Embodiment approach 3

[0046] Embodiment 3: This embodiment includes the kit of primers described in Embodiment 1.

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Abstract

The invention discloses cloning and application of a poa pratensis glutamine synthetase gene PpGS1.1 promoter, relates to the field of gene engineering, and aims to fill the blank of research on a poa pratensis PpGS1.1 promoter sequence in existing resources and lay a foundation for improving poa pratensis nitrogen absorption and utilization capacity and improving the turf use quality of poa pratensis. According to the primer disclosed by the invention, the specific primer is SP1: 5 '-GCGGCCATGACAAGAATAAGAAG-3'; sP2: 5 '-GCGATGATCTTCTTCGGTGGTGTT-3', and SP3: 5 '- SP3: 5 '-AGATCGGTGAGGAGCGCCCATAA-3', and SP3: 5 '- And the SP4 is 5 '-TCGAACCGATGTACCGTATATGG-3', and the SP4 is 5 '- The nucleotide sequence of the promoter fragment of the PpGS1.1 gene is as shown in SEQ ID NO. 1.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to the cloning and application of a PpGS1.1 promoter of a bluegrass glutamine synthase gene. Background technique [0002] Poa pratensis (scientific name: Poa pratensis L., English name: Ken tuckybluegrass) is a perennial cool-season rhizome-sparse clump grass belonging to the Poaceae (Gramineae) genus Poa L. The cool-season turfgrass has good properties and high ornamental value. Adequate nitrogen nutrition is an important factor that directly affects the quality of turfgrass plants. As a widely used turfgrass plant in the three northern regions of my country (including northeast, northwest and north China), a large amount of nitrogen fertilizer needs to be applied to maintain the ornamental quality of bluegrass turfgrass. . Improving the nitrogen utilization efficiency of bluegrass is not only beneficial to improve its quality and ornamental value, but also has i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/10
CPCC12N9/93C12N15/10C12Y603/01002C12Q2531/113C12Q2565/125Y02P60/21
Inventor 谢福春李勋陈雅君崔国文秦立刚胡国富李冰张攀孙晓阳
Owner NORTHEAST AGRICULTURAL UNIVERSITY