Cloning and application of Poa pratensis glutamine synthetase gene PpGS1.1 promoter
A technology of glutamine and bluegrass, applied in the field of biotechnology breeding, to improve nitrogen absorption and utilization, and enhance transcriptional regulation
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specific Embodiment approach 1
[0040]Embodiment 1: a kind of primer of the present embodiment for cloning the promoter fragment of the bluegrass glutamine synthase PpGS1.1 gene, the primer sequence is as follows:
[0041] SP1: 5'-GCGGCCATGACAAGAATAAGAAG-3';
[0042] SP2: 5'-GCGATGATCTTCTCGGTGGTGT-3';
[0043] SP3: 5'-AGATCGTGAGGAGCGCCATAA-3';
[0044] SP4: 5'-TCGAACCGATGTACCGTATATGG-3'.
specific Embodiment approach 2
[0045] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the nucleotide sequence of the promoter fragment of the PpGS1.1 gene is shown in SEQ ID NO.1. Others are the same as the first embodiment.
specific Embodiment approach 3
[0046] Embodiment 3: This embodiment includes the kit of primers described in Embodiment 1.
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