51 results about "Colletotrichum sp." patented technology

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A118 and application thereof. The bacillus velezensis A118 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on colletotrichum capsici. The bacillus velezensis A118 disclosed by the invention also has relatively strong capability of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on chilies, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of pepper diseases is reduced, the pepper growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

This invention discloses the CAPE1 peptide at the C-terminus of the PtPR1 protein and its applications. There are three types of CAPE1 peptides in the PtPR1 protein: PtPR1f (amino acid sequence shown in SEQ ID NO.1), PtPR1j (amino acid sequence shown in SEQ ID NO.2), and PtPR1l (amino acid sequence shown in SEQ ID NO.3). Experimental results show that the three CAPE1 peptides at the C-terminus of the PtPR1 protein provided by this invention can induce an immune defense response in woody plants at low concentrations. The use of these three CAPE1 peptides can also significantly improve the disease resistance of woody plants to *Colletotrichum gloeosporioides*, *Pseudomonas aeruginosa*, *Botrytis cinerea*, or *Phytophthora spp.*, without inhibiting the growth of these pathogens. Therefore, it can be applied to the prevention and control of woody plant diseases, providing a new approach to improving the disease resistance of woody plants and showing broad application prospects in the green control of woody plant diseases.

The application relates to the field of agricultural disease control technology, and particularly relates to the use of indole compounds and pharmaceutically acceptable salts, solvates, crystal forms, prodrugs, stereoisomers and tautomers thereof for preparing a medicine for inhibiting pathogenic key protein kinase CgSlt2 of pathogenic fungi. The application first proposes a new type of CgSlt2-targeted inhibitor, specifically, indole compounds ((S)-N-(1-(1H-indol-3-yl)-4-(methylamino)-4-oxobutan-2-yl)-8-bromo-1,6-naphthyridine-2-carboxamide) and ((S)-N-(2-((1H-indol-3-yl)-1-(methylamino)-1-oxopropyl)-8-bromo-1,6-naphthyridine-2-carboxamide) are used as CgSlt2 inhibitors, have the characteristics of high target specificity and high activity, and exhibit excellent pathogenicity inhibition of a strain of the complex group of Colletotrichum gloeosporioides.

The invention provides an application of a CsSCPL36 gene, an encoded protein or a derivative thereof in regulating resistance of plants to anthracnose, and belongs to the technical field of biology. The invention provides a CsSCPL36 gene, an encoding protein of the CsSCPL36 gene or an application of a gene derivative containing the CsSCPL36 gene in regulation and control of resistance of plants to anthracnose and / or cultivation of anthracnose-resistant plant varieties. The CsSCPL36 gene or the CsSCPL36 gene coding protein can be used for improving the resistance of plants to anthracnose through forward regulation and control. According to the embodiment of the invention, by screening the influence of overexpression of different genes on anthracnose resistance, a result shows that overexpression of the CsSCPL36 gene can effectively improve the anthracnose resistance of the citrus, and a new way is provided for improving the resistance of the citrus to colletotrichum gloeosporioides.

The invention discloses paenibacillus FHY1 and application thereof, and belongs to the field of microorganisms. A strain of paenibacillus FHY1 is screened from Xinjiang populus euphratica juice, and experimental verification shows that a liquid culture of the paenibacillus FHY1 strain provided by the invention has a remarkable inhibition effect on magnaporthe oryzae, the bacteriostasis rate can reach 84%, and the inhibition rate on magnaporthe oryzae spore germination is 92%. And the rice growth can be promoted under the conditions of 150mM NaCl and 250mM NaCl. Meanwhile, the strain also has a good inhibition effect on pyricularia grisea, fusarium oxysporum, fusarium graminearum, sclerotinia sclerotiorum and colletotrichum gloeosporioides. Therefore, the invention provides a new strain with a good control effect on pathogenic fungi, and meanwhile, the growth and development of rice are remarkably promoted in a salt stress environment.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention belongs to the technical field of pesticides, and relates to 11 bactericide compositions for mainly preventing and treating crop diseases caused by pyricularia oryzae and colletotrichum gloeosporioides. The compound YJY-22 provided by the invention is novel in structure and unique in action mode, and has no cross resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil and tebuconazole. As the action mechanism of the compound YJY-22 is different from that of the medicament, and the compound YJY-22 and the medicament have no cross resistance, the compound YJY-22 and the medicament are compounded, so that the sterilization range can be expanded, the sterilization effect can be enhanced, the drug resistance of phytopathogen can be delayed, the safety to crops is good, the prevention and control spectrum is expanded, and the requirements of pesticide reduction and synergism are met.

The invention belongs to the technical field of RNA biological prevention and treatment, and particularly relates to application of dsRNA of a targeted actin coding gene. The invention provides application of dsRNA of a targeted actin coding gene in prevention and treatment of crop anthracnose, the nucleotide sequence of the dsRNA is shown as SEQ ID No.2, the dsRNA is obtained by transcription of a target gene segment of a colletotrichum CaACTIN gene, and the nucleotide sequence of the target gene segment of the colletotrichum CaACTIN gene is shown as SEQ ID No.1. The dsRNA is loaded by a nano-material layered double hydroxide, so that the dsRNA can be applied to prevention and treatment of crop anthracnose, and the target gene segment of the colletotrichum CaACTIN gene is obtained through transcription of the target gene segment of the colletotrichum CaACTIN gene and the target gene segment of the colletotrichum CaACTIN gene. The pathogenicity of mango colletotrichum gloeosporioides and banana colletotrichum gloeosporioides can be obviously reduced, and the application prospect of efficient and targeted control of crop anthracnose is achieved.

The application discloses a strain of Burkholderia gladioli CT-5 and application thereof, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20242732, and the preservation date is December 5, 2024. The Burkholderia gladioli CT-5 provided by the application has a significant prevention and treatment effect (P<0.05) on root rot of Idesia polycarpa caused by Fusarium solani, and has a significant inhibitory effect on other various plant pathogenic fungi such as Schizophyllum commune, Diaporthe eres and Colletotrichum acutatum. In a pot experiment, the biomass of Idesia polycarpa seedlings, soil nutrients, SOD, POD and CAT enzyme activities of the CT-5 treatment group are significantly improved (P<0.05) compared with the pathogenic fungus control group (FP), indicating that the strain can promote plant growth, activate the activity of defense enzymes in the body, and thus effectively control the root rot of Idesia polycarpa, which provides strong theoretical basis and technical support for the sustainable development of Idesia polycarpa.

The invention provides a strain of Chryseobacterium ZJU645, a biological preparation, a culture method, a fermentation method and application, and particularly belongs to the technical field of microbial control. The Chryseobacterium sp. ZJU645 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.35186, and the preservation date is July 10, 2025. The Chryseobacterium ZJU645 disclosed by the invention has a broad-spectrum antifungal effect, and has a relatively high antibacterial effect on alternaria alternata, sclerotinia sclerotiorum, fusarium oxysporum, paniculate corn, colletotrichum gloeosporioides and botrytis cinerea, and a fermentation product of the Chryseobacterium ZJU645 is stable. Through further optimization of fermentation conditions of the Chryseobacterium sp. ZJU645, the disease resistance of the Chryseobacterium sp. ZJU645 is improved, and a new biological control means is provided for more efficient prevention and treatment of agricultural diseases, especially mulberry diseases.

The invention belongs to the technical field of microorganisms, and particularly discloses a bacillus aryabhattai strain and application thereof, the bacillus aryabhattai strain is identified as the bacillus aryabhattai strain through morphology, physiology, biochemistry and 16S rDNA (ribosomal deoxyribonucleic acid) molecules, is named as GR-6, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.37494. The bacillus aryabhattai GR-6 disclosed by the invention has an in-vitro control effect of 67.58% and a living potting control effect of 38.67% on tomato gray leaf spot disease, and has good nitrogen fixation and potassium solubilization capabilities, and generated volatile substances have an obvious inhibition effect on various plant pathogenic fungi such as Stemphylium solani, Corynespora cassiicola, Botryis cinerea, fusarium oxysporum and Colletotrichum capsici; according to the present invention, the genome contains Surfactin, Macrolactin, Difficidin and other lipopeptide and polyketone antibiotic synthesis related genes, such that the biocontrol effect is significant, the growth promoting function and the broad-spectrum antibacterial ability are provided, the environmental protection is provided, the new microbial resource is provided for the green prevention and control of the tomato gray leaf spot disease, and the important application development value is provided.

The invention discloses application of mortierella 2K4 as a biocontrol bacterium for poplar anthracnose, and belongs to the technical field of microbial pesticides. A plate confrontation experiment proves that the strain has a remarkable antagonistic effect on poplar anthracnose pathogenic bacteria colletotrichum gloeosporioides, and the bacteriostasis rate reaches 50.9%. The fermentation filtrate also shows a concentration-dependent antibacterial effect, and the antibacterial rate of 50% concentration is 45.50%. In-vitro leaf and pot experiments further prove that the scab diameter and disease index of the poplar anthracnose can be effectively reduced by applying the strain, and the prevention and treatment effect exceeds 59.45%. The invention provides an efficient and environment-friendly biocontrol bacterium resource for green prevention and control of poplar anthracnose and an application method of the biocontrol bacterium resource.

11 fungicide compositions mainly used for controlling crop diseases caused by Pyricularia oryzae and Colletotrichum spp.. Compound YJY-22 has a novel structure and a unique mode of action, and exhibits no cross-resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, or tebuconazole. Because compound YJY-22 and the described agents have different mechanisms of action and have no cross-resistance, combining compound YJY-22 and the described agents not only can broaden the antifungal spectrum and enhance antifungal efficacy, but also can delay fungicide resistance of plant pathogens, ensure high crop safety, expand the control spectrum, and meet the requirement of pesticide reduction with increased efficiency.

The invention belongs to the technical field of medicinal chemistry and agricultural biology, and particularly relates to a pyrone natural product with antifungal activity and a preparation method and application thereof. According to the invention, a novel pyrone natural product is separated from the plant endophytic fungus Diamorpha kushuensis ZMU-48-1 for the first time, and the novel pyrone natural product can be obtained. According to the present invention, the compound has significant inhibitory activity on colletotrichum musae (Colletotrichum gloeosporioides) and colletotrichum gloeosporioides (Colletotrichum gloeosporioides), and can be used for the preparation of the compound, such that the compound has significant inhibitory activity on colletotrichum musae (Colletotrichum gloeosporioides), compared with traditional chemical synthetic fungicides, the compound has the outstanding advantages of novel structure, strong targeting property, environmental friendliness, slow resistance development and the like, has a good prospect in the field of research and development of agricultural antifungal drugs, is particularly suitable for green control of plant fungal diseases, and has important scientific research value and application value.

This invention provides the application of Evodia rutaecarpa root extract in the preparation of antifungal agents, belonging to the field of agricultural fungal control technology. The active ingredient of the Evodia rutaecarpa root extract is naturally derived and easily obtained; its unique chemical structure and novel mechanism of action effectively avoid the problem of pathogen resistance caused by the concentrated mechanisms of action of existing fungicides, filling the market gap for quinolone-based plant-derived pesticides. The Evodia rutaecarpa root extract exhibits a precise antifungal spectrum and significant inhibitory effect, showing obvious inhibitory activity against soil-borne and interplant-borne plant fungi that are serious threats in agricultural production, such as Phytophthora tobaccoii, Fusarium equisetifolium, Colletotrichum gloeosporioides, and Fusarium moniliforme.

The invention belongs to the technical field of plant disease and insect pest prevention and control, and particularly relates to application of nerol in prevention and control of rubber anthracnose. It is found for the first time that nerol with low concentration (0.5 [mu] L / mL) can effectively inhibit colletotrichum gloeosporioides, phytophthora capsici and colletotrichum gloeosporioides, and especially the inhibition rate of nerol to colletotrichum gloeosporioides and colletotrichum gloeosporioides can reach 100%. Besides, nerol also has obvious inhibitory activity on rubber anthrax bacteria inoculated on in-vitro leaves, and can obviously reduce the area of scabs, and the inhibition rate reaches 98.4%. The results show that the nerol has a good prevention and treatment effect on the rubber anthracnose, the nerol belongs to a plant-sourced volatile compound, and few residues are left when the nerol is used for preventing and treating the rubber tree anthracnose, so that the nerol can be used as a potential green pesticide for preventing and treating the rubber anthracnose, and a new way is provided for preventing and treating the rubber anthracnose.

The invention discloses colletotrichum 7060 #, belongs to the technical field of microorganisms, and is used for solving the problems of low survival rate of aquilaria sinensis seedlings, long growth cycle and lack of growth-promoting microorganism resources. According to the technical scheme, the colletotrichum 7060 # with the preservation number of CGMCC NO.42267 and the biological product of the colletotrichum 7060 # and the method for promoting agilawood growth are provided, and the method is achieved through strain activation, cake making and symbiotic culture. Preferred schemes involve addition of a host root extract, staged supply of terpene signal molecules, use of a controlled release device with a buffer layer, strain pre-adaptation and addition of an energy slow release component. The agilawood growth can be effectively promoted, the biomass and stress resistance are improved, and reliable microbial resources and technical support are provided for agilawood seedling culture.

This disclosure provides microbial consortia to target Colletotrichum or Botrytis species that target flowers, such as those of blueberries, to control diseases caused by these phytopathogenic species. Methods of delivery of the consortia to plants, including pre-blossom and post-blossom stages.

The bacillus velezensis strain EMM914 is preserved in the China Center for Type Culture Collection on May 19, 2025, and the preservation number of the bacillus velezensis strain EMM914 is CCTCC (China Center for Type Culture Collection) NO: M 20251091. The nucleotide sequence comprises a 16S rRNA (ribosomal Ribonucleic Acid) sequence and a gyrB sequence, which are as shown in SEQ ID No. 1 and SEQ ID No. 2. The strain EMM914 can be used for preparing medicines for preventing and treating fusarium moniliforme EMF994, fusarium oxysporum EMF889, colletotrichum gloeosporioides EMF991, fusarium graminearum EMF897, fusarium laminatum EMF998, black beancurd skin fungus EMF990, fusarium solani EMF893, fusarium oxysporum EMF996, botryosphaeria dothidea EMF899, fusarium solani EMF895, fusarium trifida EMF997, pear black beancurd skin fungus EMFB1, fusarium oxysporum EMF894, fusarium trifida EMF896, alternaria alternata EMF993, fusarium solani EMF1001, fusarium According to the application of the compound in microbial pesticides for any fungal disease in botrytis cinerea EMFHM, the inhibition rate eta of 19 pathogenic bacteria is 65% or above.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to bacillus BFI-L3 with a broad-spectrum antibacterial effect and application of the bacillus BFI-L3. The invention discloses bacillus velezensis BFI-L3, which is bacillus velezensis, and the preservation number of the bacillus velezensis BFI-L3 is CGMCC (China General Microbiological Culture Collection Center) No.35399. The strain has broad-spectrum antibacterial activity, has a remarkable antibacterial effect on camellia anthracnose, botryosphaeria, alternaria and other pathogenic fungi, and can synchronously prevent and control the compound diseases of the tea garden. The strain also has plant growth promoting ability, VOCs generated by the strain can significantly promote the growth of arabidopsis thaliana, and high-quality microbial resources are provided for green prevention and control of tea gardens and biological prevention and control of plant diseases.

The invention discloses a tea tree CsERF9 gene and application of the tea tree CsERF9 gene in regulation and control of anthracnose resistance of tea trees. The tea tree CsERF9 gene is identified and cloned in the invention. Researches show that the gene encodes an ERF transcription factor, and the expression of the ERF transcription factor is induced by colletotrichum gloeosporioides, low temperature and drought stress. Functional verification experiments prove that overexpression of CsERF9 in tea tree leaves can significantly enhance the susceptibility of plants to colletotrichum gloeosporioides, indicating that CsERF9 is a negative regulatory factor of tea tree anthracnose resistance. Mechanism research finds that CsERF9 weakens the disease resistance of plants by inhibiting the expression of key defense genes. Therefore, the invention provides a novel method and a technical approach for improving the anthracnose resistance of the tea tree by inhibiting the activity of the CsERF9 gene or the protein of the CsERF9 gene. The invention provides a new gene target and strategy for crop disease resistance genetic improvement, and has important theoretical value and wide application prospect.

The invention relates to the technical field of agricultural biology, in particular to application of flavin adenine dinucleotide as a ribonuclease inhibitor in prevention and treatment of pear anthracnose. The invention clarifies the action effect of the natural compound flavin adenine dinucleotide (FAD) in inhibiting ribonuclease activity and preventing and treating pear anthracnose. The FAD (100 [mu] M) can be combined with ribonuclease of colletotrichum gloeosporioides and significantly inhibit the enzymatic activity of the ribonuclease, external application of the FAD can inhibit the colletotrichum gloeosporioides from infecting pears, significantly inhibit the morbidity of pear anthracnose and reduce the morbidity of pear fruits in the storage process, and the application value is good.

The invention discloses Cas9-based primers, a Cas9-based detection system, a Cas9-based detection method and a Cas9-based detection kit for colletotrichum gloeosporioides on fruits and vegetables, and the Cas9-based detection system comprises an RPA forward primer, an RPA reverse primer and an sgRNA sequence; the nucleotide sequence of the RPA forward primer is as shown in SEQ ID NO.1, the nucleotide sequence of the RPA reverse primer is as shown in SEQ ID NO.2, and the nucleotide sequence of the sgRNA sequence is as shown in SEQ ID NO.3; the detection system comprises an RPA (recombinase polymerase amplification) pre-amplification reaction mixed solution and a CRISPR-dCas9 detection mixed solution; the RPA pre-amplification reaction mixed solution contains an RPA forward primer and an RPA reverse primer; the CRISPR-dCas9 detection mixed solution contains an sgRNA (small guide ribonucleic acid) sequence and dCas9 biotinylated protein. The detection method comprises the following steps: placing a CRISPR-dCas9 detection mixed solution on a cover of a reaction tube; placing an RPA pre-amplification reaction mixed solution at the bottom of a reaction tube, and adding a DNA template solution and a magnesium acetate solution for incubation; inverting the reaction tube and continuing incubation; adding a chromatography buffer solution into the reaction tube, and inserting a lateral chromatography test strip for detection. The method can solve the problems of long time consumption and low accuracy of a traditional colletotrichum gloeosporioides morphological identification method.

The invention discloses a strain of salt-tolerant bacillus (bacillus halotolans) with a biocontrol effect as well as a microbial agent and application of the salt-tolerant bacillus halotolans. The salt-tolerant bacillus is named as bacillus halotolans 1-64 and is preserved in the China Center for Type Culture Collection, and the preservation number of the salt-tolerant bacillus is CCTCC (China Center for Type Culture Collection) NO: M20252455. Experiments prove that the strain has nitrogen fixation capacity, can generate non-volatile and volatile antibacterial metabolites with thermal stability, and has a remarkable inhibition effect on various important plant pathogenic fungi such as phytophthora nicotianae, alternaria alternata, fusarium oxysporum, penicillium expansum, botryosphaeria dothidea, colletotrichum gloeosporioides and rhizoctonia solani. The invention also optimizes the fermentation medium formula and culture conditions of the strain, and obviously improves the biomass of the strain. The strain 1-64 has a wide application prospect in the aspect of being developed into efficient, stable and environment-friendly biopesticides.

The invention discloses bacillus velezensis JN-Y2 tending to and inhibiting colletotrichum graminearum spores and application of the bacillus velezensis JN-Y2, and belongs to the technical field of biological control. The preservation number of the bacillus velezensis JN-Y2 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 35094. The bacillus velezensis JN-Y2 tends to the oat anthracnose pathogenic bacterium colletotrichum graminearum, has a good prevention and treatment effect on the oat anthracnose, and has a good development and application prospect. Compared with a chemical agent, the JN-Y2 bacterial agent has the advantages of being not prone to generating drug resistance, safe to human and livestock, free of pesticide residues and the like.

The invention belongs to the technical field of fermentation engineering and plant disease prevention and control, and particularly relates to pseudomonas veronii and application thereof in prevention and control of plant fungal diseases. The invention discloses Pseudomonas veronii JD003 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36825. The test shows that the Pseudomonas veronii JD003 can effectively inhibit the growth of tomato root rot, grape botrytis cinerea, colletotrichum capsici and alternaria capsici, and can effectively inhibit the growth of the tomato root rot, the grape botrytis cinerea, the colletotrichum capsici and the alternaria capsici. The compound provided by the invention has the advantages that the inhibition effects on the corresponding pathogenic microorganisms such as fusarium solani, botrytis cinerea, colletotrichum oxysporum and alternaria hexabarzicola respectively reach 64.7%, 72.9%, 74.1% and 73.8%, the compound has a good inhibition effect on pepper anthracnose, the field prevention and control effect reaches 83.5%, and a new direction is found for preventing and controlling plant diseases.

The invention discloses a preparation method of a lipopeptide mixture and an antibacterial effect determination method, relates to the technical field of lipopeptide mixtures, and realizes efficient preparation and accurate identification of the lipopeptide mixture by optimizing fermentation, separation and purification processes of bacillus velezensis TZ01 and combining genome sequencing and liquid chromatography-mass spectrometry technologies. The antibacterial effect of the lipopeptide mixture is systematically verified, the provided preparation method of the lipopeptide mixture is clear in process and mild in condition, complex equipment and harsh reaction conditions are not needed, accurate identification of lipopeptide components is achieved through a genomic sequencing and liquid chromatography and high-resolution mass spectrometry combined technology, and the application prospect is wide. According to the present invention, the antibacterial spectrum covers the main citrus pathogenic bacteria such as Pseudomonas solani, Alternaria alternata, Colletotrichum gloeosporioides, Penicillium digitatum, Penicillium italicum and the like, such that the requirement of the comprehensive prevention and control of various citrus diseases can be met, the defect of the narrow antibacterial spectrum of the existing lipopeptide antibacterial agent is solved, the lipopeptide substance is derived from the microbial metabolism, the chemical pesticide residue risk does not exist, and the application prospect is broad. Ecological environments such as soil and water cannot be polluted.

This invention discloses a class of imidazo[1,2-]azoline containing a hydrazone structure. a Preparation methods and applications of pyridine derivatives, this invention uses imidazo[1,2- a Using the pyridine skeleton as the parent structure, a series of imidazo[1,2-] hydrazone fragments were introduced to design and synthesize a series of imidazo[1,2-] hydrazone-containing structures. a Pyridine derivatives. In vitro activity results showed that these compounds had excellent inhibitory activity against plant pathogenic fungi (such as *Botrytis cinerea*, *Colletotrichum gloeosporioides*, *Colletotrichum sorghum*, *Colletotrichum tea*, *Alternaria alternata*, and *Rhizoctonia solani*), and some of the target compounds also showed good inhibitory activity against plant pathogenic bacteria.

The invention relates to application of streptomyces mnorhizosphere and fermentation liquor thereof in inhibition of colletotrichum truncatum resisting picolinamide, and belongs to the technical field of biological prevention and control of crop diseases. The biocontrol strain 14-3 is named as streptomyces farnesii, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No. 63693. The strain shows remarkable antagonistic activity on colletotrichum truncatum with picolinamide resistance, and fermentation liquor of the strain can effectively inhibit mycelial growth of pathogenic bacteria, germination of conidia and formation of attachment spores and can cause spore splitting decomposition. In addition, fermentation liquor of the strain has a good prevention and treatment effect on soybean anthracnose caused by infection of colletotrichum truncatum resisting picolinamide, and shows good application potential in the aspect of crop drug resistance treatment.