92 results about "Colletotrichum sp." patented technology

The invention discloses a Burkholderia gladioli (Burkholderia gladioli) CT-5 strain and an application of the Burkholderia gladioli CT-5 strain, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242732, and the preservation date is December 05, 2024. The Burkholderia gladioli (Burkholderia gladioli) CT-5 provided by the invention has a remarkable prevention and treatment effect (Plt, Plt) on idesia root rot caused by Fusarium solani (Fusarium solani), and the Burkholderia gladioli (Burkholderia gladioli) CT-5 has a remarkable prevention and treatment effect on idesia root rot caused by Fusarium solani (Busarium solani). The compound has the advantages that the compound can be used for inhibiting pathogenic bacteria of various plants, and the compound can be used for inhibiting pathogenic bacteria of various plants, such as Schizophyllum commune, Diaporthe erens, Colletotrichum acutatum and the like, and has a remarkable inhibiting effect on other various plant pathogenic bacteria such as the Schizophyllum commune, the Diaporthe erens, the Colletotrichum acutatum and the like. In a pot experiment, the biomass, soil nutrients, SOD, POD and CAT enzyme activities of idesia polycarpa seedlings in a CT-5 treatment group are remarkably improved compared with those of a pathogenic bacteria control group (FP) (Plt; 0.05), which indicates that the strain can promote plant growth and activate the activity of defensive enzyme in the body, so that effective control of idesia root rot is realized, and powerful theoretical basis and technical support are provided for sustainable development of idesia.

The invention discloses bacillus amyloliquefaciens GX0003936 and application thereof, and belongs to the technical field of microorganism application. The invention discloses bacillus amyloliquefaciens GX0003936 with a broad-spectrum bacteriostatic effect, which is preserved in the China General Microbiological Culture Collection Center on March 26, 2025, and the preservation number of the bacillus amyloliquefaciens GX0003936 is CGMCC (China General Microbiological Culture Collection Center) No.33987. The bacterial strain has broad-spectrum antibacterial activity, can be used for remarkably inhibiting the growth of mycelia of colletotrichum musae and colletotrichum frutescens, and can be used for remarkably reducing the disease incidence rate of tropical fruits such as bananas, mangoes and the like in the postharvest storage period. Meanwhile, the strain shows a good inhibition effect on various plant pathogenic fungi, and has a potential broad-spectrum biocontrol effect.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A118 and application thereof. The bacillus velezensis A118 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on colletotrichum capsici. The bacillus velezensis A118 disclosed by the invention also has relatively strong capability of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on chilies, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of pepper diseases is reduced, the pepper growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating idesia anthracnose, fermentation liquor, a biocontrol bacterium agent and application. According to the invention, a biocontrol bacterium is separated and screened from microbial resources on healthy idesia leaves, is classified and named as Bacillus velezensis, is preserved in China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided by the invention has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, botrytis cinerea, fusarium oxysporum, alternaria alternata and pestalotiopsis which can cause plant diseases, and also has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, fusarium oxysporum, alternaria alternata and pestalotiopsis. The biocontrol bacterium, the fermentation broth and the biocontrol inoculant provided by the invention have potential application prospects in prevention and treatment of idesia anthracnose.

The invention provides a stenotrophomonas sp. CLX-022 strain which has a good antagonistic effect on colletotrichum gloeosporioides and fusarium fusiforme, and the strain is preserved in China Center for Type Culture Collection (address: Wuhan University, Wuhan, China) and has a good antagonistic effect on colletotrichum gloeosporioides and fusarium fusiforme. The strain is preserved in China Center for Type Culture Collection (CCTCC) NO: M20252110 on September 24, 2025, the postal code is 430072, and the preservation number is CCTCC NO: M20252110. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The stenotrophomonas CLX-022 strain is high in antibacterial property and can be used for preparing a microbial preparation for treating / preventing strawberry anthracnose and rice bakanae disease.

The invention discloses application of a CsNOMT gene and sakuranetin in prevention and treatment of tea tree anthracnose. It is found for the first time that methyltransferase encoded by the tea tree-derived CsNOMT gene can efficiently catalyze naringenin to be converted into sakuranetin, the endogenous phytoalexin level of a tea tree is remarkably improved, and then the disease resistance of the tea tree-derived CsNOMT gene to Colletotrichum carolinae is enhanced. The silence of the CsNOMT gene can reduce the sakuranetin content by 30-40%, resulting in the increase of the scab area; and by overexpressing the gene, the sakuranetin content is increased by 30%, and the scab area is remarkably reduced. In addition, the growth of colletotrichum can be effectively inhibited by exogenously spraying 0.05-0.3 mM of sakuranetin, and the optimal concentration is 0.1-0.3 mM. The invention provides a new strategy for green prevention and control of tea tree anthracnose, and has the characteristics of high efficiency, safety and environmental friendliness.

The invention discloses bacillus subtilis Bs1 as well as an antagonistic substance and application thereof. According to the invention, Bs1 with antagonism on colletotrichum gloeosporioides is separated and screened for the first time, an antagonistic substance is clear as Bs1 serine-producing protease Vpr, a Bs1 bacteriostasis mechanism and biological control potential excavation are analyzed in a multi-omics manner, a peripheral colonization rule of Bs1-Rif is clear, and an indoor control strategy of the strain Bs1 on cunninghamia lanceolata anthracnose is optimized. When the strain Bs1 is applied on the day when pathogenic bacteria are inoculated, a certain prevention and treatment effect is achieved, and it is indicated that the strain Bs1 can rapidly occupy the ecological niche; the average antibacterial rate of the strain Bs1 can reach 66.69%. The biocontrol strain and theoretical basis are provided for prevention and control of Chinese fir diseases, the application of biological prevention and control in forestry is promoted, the interaction mechanism of biocontrol bacteria and pathogenic bacteria can be deeply understood, and the sustainable development requirement of modern forestry is met.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

This invention discloses the CAPE1 peptide at the C-terminus of the PtPR1 protein and its applications. There are three types of CAPE1 peptides in the PtPR1 protein: PtPR1f (amino acid sequence shown in SEQ ID NO.1), PtPR1j (amino acid sequence shown in SEQ ID NO.2), and PtPR1l (amino acid sequence shown in SEQ ID NO.3). Experimental results show that the three CAPE1 peptides at the C-terminus of the PtPR1 protein provided by this invention can induce an immune defense response in woody plants at low concentrations. The use of these three CAPE1 peptides can also significantly improve the disease resistance of woody plants to *Colletotrichum gloeosporioides*, *Pseudomonas aeruginosa*, *Botrytis cinerea*, or *Phytophthora spp.*, without inhibiting the growth of these pathogens. Therefore, it can be applied to the prevention and control of woody plant diseases, providing a new approach to improving the disease resistance of woody plants and showing broad application prospects in the green control of woody plant diseases.

The application relates to the field of agricultural disease control technology, and particularly relates to the use of indole compounds and pharmaceutically acceptable salts, solvates, crystal forms, prodrugs, stereoisomers and tautomers thereof for preparing a medicine for inhibiting pathogenic key protein kinase CgSlt2 of pathogenic fungi. The application first proposes a new type of CgSlt2-targeted inhibitor, specifically, indole compounds ((S)-N-(1-(1H-indol-3-yl)-4-(methylamino)-4-oxobutan-2-yl)-8-bromo-1,6-naphthyridine-2-carboxamide) and ((S)-N-(2-((1H-indol-3-yl)-1-(methylamino)-1-oxopropyl)-8-bromo-1,6-naphthyridine-2-carboxamide) are used as CgSlt2 inhibitors, have the characteristics of high target specificity and high activity, and exhibit excellent pathogenicity inhibition of a strain of the complex group of Colletotrichum gloeosporioides.

The invention provides an application of a CsSCPL36 gene, an encoded protein or a derivative thereof in regulating resistance of plants to anthracnose, and belongs to the technical field of biology. The invention provides a CsSCPL36 gene, an encoding protein of the CsSCPL36 gene or an application of a gene derivative containing the CsSCPL36 gene in regulation and control of resistance of plants to anthracnose and / or cultivation of anthracnose-resistant plant varieties. The CsSCPL36 gene or the CsSCPL36 gene coding protein can be used for improving the resistance of plants to anthracnose through forward regulation and control. According to the embodiment of the invention, by screening the influence of overexpression of different genes on anthracnose resistance, a result shows that overexpression of the CsSCPL36 gene can effectively improve the anthracnose resistance of the citrus, and a new way is provided for improving the resistance of the citrus to colletotrichum gloeosporioides.

The invention discloses paenibacillus FHY1 and application thereof, and belongs to the field of microorganisms. A strain of paenibacillus FHY1 is screened from Xinjiang populus euphratica juice, and experimental verification shows that a liquid culture of the paenibacillus FHY1 strain provided by the invention has a remarkable inhibition effect on magnaporthe oryzae, the bacteriostasis rate can reach 84%, and the inhibition rate on magnaporthe oryzae spore germination is 92%. And the rice growth can be promoted under the conditions of 150mM NaCl and 250mM NaCl. Meanwhile, the strain also has a good inhibition effect on pyricularia grisea, fusarium oxysporum, fusarium graminearum, sclerotinia sclerotiorum and colletotrichum gloeosporioides. Therefore, the invention provides a new strain with a good control effect on pathogenic fungi, and meanwhile, the growth and development of rice are remarkably promoted in a salt stress environment.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention belongs to the technical field of pesticides, and relates to 11 bactericide compositions for mainly preventing and treating crop diseases caused by pyricularia oryzae and colletotrichum gloeosporioides. The compound YJY-22 provided by the invention is novel in structure and unique in action mode, and has no cross resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil and tebuconazole. As the action mechanism of the compound YJY-22 is different from that of the medicament, and the compound YJY-22 and the medicament have no cross resistance, the compound YJY-22 and the medicament are compounded, so that the sterilization range can be expanded, the sterilization effect can be enhanced, the drug resistance of phytopathogen can be delayed, the safety to crops is good, the prevention and control spectrum is expanded, and the requirements of pesticide reduction and synergism are met.

The invention belongs to the technical field of RNA biological prevention and treatment, and particularly relates to application of dsRNA of a targeted actin coding gene. The invention provides application of dsRNA of a targeted actin coding gene in prevention and treatment of crop anthracnose, the nucleotide sequence of the dsRNA is shown as SEQ ID No.2, the dsRNA is obtained by transcription of a target gene segment of a colletotrichum CaACTIN gene, and the nucleotide sequence of the target gene segment of the colletotrichum CaACTIN gene is shown as SEQ ID No.1. The dsRNA is loaded by a nano-material layered double hydroxide, so that the dsRNA can be applied to prevention and treatment of crop anthracnose, and the target gene segment of the colletotrichum CaACTIN gene is obtained through transcription of the target gene segment of the colletotrichum CaACTIN gene and the target gene segment of the colletotrichum CaACTIN gene. The pathogenicity of mango colletotrichum gloeosporioides and banana colletotrichum gloeosporioides can be obviously reduced, and the application prospect of efficient and targeted control of crop anthracnose is achieved.

The invention provides a preparation method and application of bamboo fungus sourced sulfonic group modified carbon quantum dots, and belongs to the technical field of preparation of nano luminescent materials. The carbon quantum dot is prepared by taking bamboo fungus pileus as a carbon source and sodium sulfamate as a modifier through a hydrothermal method. The carbon quantum dot provided by the invention has a very strong inhibition effect on mango colletotrichum gloeosporioides. According to the invention, biological resources are effectively utilized, a foundation is laid for prevention and treatment of phytopathogen, and the application prospect is wide.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer combination, a kit and application of the LAMP detection primer combination and the kit. Six specific LAMP primer combinations are designed by utilizing a section of specific gene sequence of camellia anthracnose, and an LAMP rapid detection method of camellia anthracnose is established on the basis and is applied to field detection. The method has the advantages of rapidness, high efficiency, high accuracy, high sensitivity, capability of observing detection results by naked eyes and the like.

The invention discloses application of a sorghum SbPGK1 gene in improving plant disease resistance, and belongs to the technical field of biology. The nucleotide sequence of the sorghum SbPGK1 gene is as shown in SEQ ID NO.9. By analyzing phenotypes and related physiological indexes of SbPGK1 rice transgenic lines before and after inoculation of different pathogenic bacteria for infection and sorghum, the results show that compared with non-SbPGK1 gene rice lines, the SbPGK1 transgenic lines have obviously better resistance to magnaporthe oryzae and bacterial blight germs than wild types, silence SbPGK1 in sorghum has better resistance to rice blast germs and bacterial blight germs, and the SbPGK1 gene has better resistance to rice blast germs and bacterial blight germs. The SbPGK1 gene has the advantages that the SbPGK1 silent strain has reduced disease resistance to colletotrichum gloeosporioides, the SbPGK1 is overexpressed in sorghum, and the SbPGK1 overexpressed strain has enhanced disease resistance to colletotrichum gloeosporioides, so that the SbPGK1 gene is a potential disease-resistant breeding gene and can be used for plant disease-resistant genetic improvement.

The application discloses a strain of Burkholderia gladioli CT-5 and application thereof, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20242732, and the preservation date is December 5, 2024. The Burkholderia gladioli CT-5 provided by the application has a significant prevention and treatment effect (P<0.05) on root rot of Idesia polycarpa caused by Fusarium solani, and has a significant inhibitory effect on other various plant pathogenic fungi such as Schizophyllum commune, Diaporthe eres and Colletotrichum acutatum. In a pot experiment, the biomass of Idesia polycarpa seedlings, soil nutrients, SOD, POD and CAT enzyme activities of the CT-5 treatment group are significantly improved (P<0.05) compared with the pathogenic fungus control group (FP), indicating that the strain can promote plant growth, activate the activity of defense enzymes in the body, and thus effectively control the root rot of Idesia polycarpa, which provides strong theoretical basis and technical support for the sustainable development of Idesia polycarpa.

The invention provides a strain of Chryseobacterium ZJU645, a biological preparation, a culture method, a fermentation method and application, and particularly belongs to the technical field of microbial control. The Chryseobacterium sp. ZJU645 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.35186, and the preservation date is July 10, 2025. The Chryseobacterium ZJU645 disclosed by the invention has a broad-spectrum antifungal effect, and has a relatively high antibacterial effect on alternaria alternata, sclerotinia sclerotiorum, fusarium oxysporum, paniculate corn, colletotrichum gloeosporioides and botrytis cinerea, and a fermentation product of the Chryseobacterium ZJU645 is stable. Through further optimization of fermentation conditions of the Chryseobacterium sp. ZJU645, the disease resistance of the Chryseobacterium sp. ZJU645 is improved, and a new biological control means is provided for more efficient prevention and treatment of agricultural diseases, especially mulberry diseases.

The invention belongs to the technical field of microorganisms, and particularly discloses a bacillus aryabhattai strain and application thereof, the bacillus aryabhattai strain is identified as the bacillus aryabhattai strain through morphology, physiology, biochemistry and 16S rDNA (ribosomal deoxyribonucleic acid) molecules, is named as GR-6, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.37494. The bacillus aryabhattai GR-6 disclosed by the invention has an in-vitro control effect of 67.58% and a living potting control effect of 38.67% on tomato gray leaf spot disease, and has good nitrogen fixation and potassium solubilization capabilities, and generated volatile substances have an obvious inhibition effect on various plant pathogenic fungi such as Stemphylium solani, Corynespora cassiicola, Botryis cinerea, fusarium oxysporum and Colletotrichum capsici; according to the present invention, the genome contains Surfactin, Macrolactin, Difficidin and other lipopeptide and polyketone antibiotic synthesis related genes, such that the biocontrol effect is significant, the growth promoting function and the broad-spectrum antibacterial ability are provided, the environmental protection is provided, the new microbial resource is provided for the green prevention and control of the tomato gray leaf spot disease, and the important application development value is provided.

The invention discloses application of mortierella 2K4 as a biocontrol bacterium for poplar anthracnose, and belongs to the technical field of microbial pesticides. A plate confrontation experiment proves that the strain has a remarkable antagonistic effect on poplar anthracnose pathogenic bacteria colletotrichum gloeosporioides, and the bacteriostasis rate reaches 50.9%. The fermentation filtrate also shows a concentration-dependent antibacterial effect, and the antibacterial rate of 50% concentration is 45.50%. In-vitro leaf and pot experiments further prove that the scab diameter and disease index of the poplar anthracnose can be effectively reduced by applying the strain, and the prevention and treatment effect exceeds 59.45%. The invention provides an efficient and environment-friendly biocontrol bacterium resource for green prevention and control of poplar anthracnose and an application method of the biocontrol bacterium resource.

The invention discloses an application of fenbendazole as an agricultural fungicide in plant diseases, fenbendazole has a remarkable inhibition effect on botryosphaeria dothidea, rice sheath blight, tea tree colletotrichum and colletotrichum gloeosporioides, and is superior to positive control drugs thiabendazole and azoxystrobin. The inhibition rate of fenbendazole on the two rice sheath blight fungi is 100% under the concentration of 25 micrograms / mL. EC50 values are respectively 0.0093 [mu] g / mL and 0.0015 [mu] g / mL, and a remarkable inhibition effect is achieved. The EC50 of the compound for colletotrichum gloeosporioides and the EC50 of the compound for colletotrichum gloeosporioides are 0.085 mu g / mL and 0.18

11 fungicide compositions mainly used for controlling crop diseases caused by Pyricularia oryzae and Colletotrichum spp.. Compound YJY-22 has a novel structure and a unique mode of action, and exhibits no cross-resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, or tebuconazole. Because compound YJY-22 and the described agents have different mechanisms of action and have no cross-resistance, combining compound YJY-22 and the described agents not only can broaden the antifungal spectrum and enhance antifungal efficacy, but also can delay fungicide resistance of plant pathogens, ensure high crop safety, expand the control spectrum, and meet the requirement of pesticide reduction with increased efficiency.

The invention provides a colletotrichum gloeosporioides gene deletion type strain and a huperzine A biosynthesis method. A CAO3097 gene is deleted in a genome of the strain. According to the method, a CAO3097 gene is knocked out from colletotrichum gloeosporioides by utilizing a genetic engineering means, a gloeococcus gloeosporioides culture solution is added in the culture process of colletotrichum gloeosporioides, and the capability of synthesizing huperzine A by colletotrichum gloeosporioides is obviously improved by adopting a method of applying piperidine acid. According to the method, a genetic engineering means is adopted for the first time, and the efficiency of microbial fermentation of huperzine A is improved in combination with innovation of a culture process.

The invention belongs to the technical field of medicinal chemistry and agricultural biology, and particularly relates to a pyrone natural product with antifungal activity and a preparation method and application thereof. According to the invention, a novel pyrone natural product is separated from the plant endophytic fungus Diamorpha kushuensis ZMU-48-1 for the first time, and the novel pyrone natural product can be obtained. According to the present invention, the compound has significant inhibitory activity on colletotrichum musae (Colletotrichum gloeosporioides) and colletotrichum gloeosporioides (Colletotrichum gloeosporioides), and can be used for the preparation of the compound, such that the compound has significant inhibitory activity on colletotrichum musae (Colletotrichum gloeosporioides), compared with traditional chemical synthetic fungicides, the compound has the outstanding advantages of novel structure, strong targeting property, environmental friendliness, slow resistance development and the like, has a good prospect in the field of research and development of agricultural antifungal drugs, is particularly suitable for green control of plant fungal diseases, and has important scientific research value and application value.

The invention relates to the field of tea tree planting methods, in particular to an ecological niche complementary method for interplanting camphor trees with tea trees and application of the ecological niche complementary method. The method has the following effects: 1, interplanting of the cinnamomum camphora and the tea tree has an inhibition phenomenon on growth of physalospora piricola and colletotrichum gloeosporioides; 2, tea polyphenol of tea leaves can be reduced by interplanting the camphor trees and the tea trees; 3, the soil conductivity and the content of organic matters, alkali-hydrolyzable nitrogen and available phosphorus can be improved by intercropping cinnamomum camphora in the tea garden; and 4, tea amino acids can be increased by interplanting the camphor trees and the tea trees.

This invention provides the application of Evodia rutaecarpa root extract in the preparation of antifungal agents, belonging to the field of agricultural fungal control technology. The active ingredient of the Evodia rutaecarpa root extract is naturally derived and easily obtained; its unique chemical structure and novel mechanism of action effectively avoid the problem of pathogen resistance caused by the concentrated mechanisms of action of existing fungicides, filling the market gap for quinolone-based plant-derived pesticides. The Evodia rutaecarpa root extract exhibits a precise antifungal spectrum and significant inhibitory effect, showing obvious inhibitory activity against soil-borne and interplant-borne plant fungi that are serious threats in agricultural production, such as Phytophthora tobaccoii, Fusarium equisetifolium, Colletotrichum gloeosporioides, and Fusarium moniliforme.

This study used extracts from three representative bougainvillea varieties—Pink Panther, Red Cardinal, and Red Saffron—to conduct antibacterial tests against anise anise anthracnose, bitter melon wilt, tomato gray mold, and apple rot, respectively. The corresponding pathogens were Colletotrichum gloeosporioides, Fusarium oxysporum (specific for bitter melon), Botrytis cinerea, and Coriolus fusca. The results showed that the bougainvillea flower extract at a concentration of 5 mg / mL had the best inhibitory effect against Coriolus fusca, the pathogen of apple rot, reaching 100%. This study provides a new approach for preventing and controlling apple rot and Coriolus fusca.