118 results about "Colletotrichum sp." patented technology

The invention discloses a Burkholderia gladioli (Burkholderia gladioli) CT-5 strain and an application of the Burkholderia gladioli CT-5 strain, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242732, and the preservation date is December 05, 2024. The Burkholderia gladioli (Burkholderia gladioli) CT-5 provided by the invention has a remarkable prevention and treatment effect (Plt, Plt) on idesia root rot caused by Fusarium solani (Fusarium solani), and the Burkholderia gladioli (Burkholderia gladioli) CT-5 has a remarkable prevention and treatment effect on idesia root rot caused by Fusarium solani (Busarium solani). The compound has the advantages that the compound can be used for inhibiting pathogenic bacteria of various plants, and the compound can be used for inhibiting pathogenic bacteria of various plants, such as Schizophyllum commune, Diaporthe erens, Colletotrichum acutatum and the like, and has a remarkable inhibiting effect on other various plant pathogenic bacteria such as the Schizophyllum commune, the Diaporthe erens, the Colletotrichum acutatum and the like. In a pot experiment, the biomass, soil nutrients, SOD, POD and CAT enzyme activities of idesia polycarpa seedlings in a CT-5 treatment group are remarkably improved compared with those of a pathogenic bacteria control group (FP) (Plt; 0.05), which indicates that the strain can promote plant growth and activate the activity of defensive enzyme in the body, so that effective control of idesia root rot is realized, and powerful theoretical basis and technical support are provided for sustainable development of idesia.

The invention discloses bacillus amyloliquefaciens GX0003936 and application thereof, and belongs to the technical field of microorganism application. The invention discloses bacillus amyloliquefaciens GX0003936 with a broad-spectrum bacteriostatic effect, which is preserved in the China General Microbiological Culture Collection Center on March 26, 2025, and the preservation number of the bacillus amyloliquefaciens GX0003936 is CGMCC (China General Microbiological Culture Collection Center) No.33987. The bacterial strain has broad-spectrum antibacterial activity, can be used for remarkably inhibiting the growth of mycelia of colletotrichum musae and colletotrichum frutescens, and can be used for remarkably reducing the disease incidence rate of tropical fruits such as bananas, mangoes and the like in the postharvest storage period. Meanwhile, the strain shows a good inhibition effect on various plant pathogenic fungi, and has a potential broad-spectrum biocontrol effect.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A118 and application thereof. The bacillus velezensis A118 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on colletotrichum capsici. The bacillus velezensis A118 disclosed by the invention also has relatively strong capability of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on chilies, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of pepper diseases is reduced, the pepper growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention belongs to the technical field of RNA biological prevention and treatment, and particularly relates to application of dsRNA of a targeting sphingolipid C-4 hydroxylase coding gene. The invention provides application of targeted sphingolipid C-4 hydroxylase coding gene dsRNA in prevention and treatment of crop anthracnose and / or preparation of products for prevention and treatment of crop anthracnose, and the targeted sphingolipid C-4 hydroxylase coding gene dsRNA is obtained by transcription of partial sequence of a sphingolipid synthetase coding gene CsSUR2. The targeting sphingolipid C-4 hydroxylase coding gene dsRNA is loaded on the nano layered double hydroxide, so that the pathogenicity of colletotrichum gloeosporioides and mango colletotrichum gloeosporioides can be obviously reduced, and the application prospect of preventing crop anthracnose is achieved.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating idesia anthracnose, fermentation liquor, a biocontrol bacterium agent and application. According to the invention, a biocontrol bacterium is separated and screened from microbial resources on healthy idesia leaves, is classified and named as Bacillus velezensis, is preserved in China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided by the invention has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, botrytis cinerea, fusarium oxysporum, alternaria alternata and pestalotiopsis which can cause plant diseases, and also has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, fusarium oxysporum, alternaria alternata and pestalotiopsis. The biocontrol bacterium, the fermentation broth and the biocontrol inoculant provided by the invention have potential application prospects in prevention and treatment of idesia anthracnose.

The invention belongs to the field of comprehensive utilization of palm leaves, and particularly relates to a natural preservative containing palm leaf extract as well as a preparation method and application of the natural preservative. According to the natural preservative disclosed by the invention, sunflower discs, betel nut leaves, mint leaves, oil-tea cakes and barley are added into lactobacillus plantarum to prepare fermentation liquor, the fermentation liquor is sterilized, and then palm leaf extract and purslane extract are added, so that the antibacterial rate on mango colletotrichum gloeosporioides, litchi fruit colletotrichum gloeosporioides and strawberry colletotrichum gloeosporioides can be remarkably increased; the preservative can effectively prevent fruits from decaying and deterioration, can effectively prolong the storage life of fruits when being used for preservation and fresh keeping of fruits, has small toxic and side effects, and greatly improves the safety.

The invention discloses bacillus and application thereof, and belongs to the technical field of microorganisms, the bacillus is preserved in China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20242290, and the preservation date is October 22, 2024. The composition can effectively inhibit vegetative growth and vegetative propagation of pathogenic bacteria such as colletotrichum gloeosporioides and paecilomyces japonicus, and has a good and broad-spectrum control effect on various pathogenic fungi. Bacteriostatic components contained in fermentation filtrate of the bacillus can remarkably inhibit germination of colletotrichum spruce spores and formation of appressorium. And the bacillus has a good prevention and treatment effect on the citrus huanglongbing.

The invention provides a stenotrophomonas sp. CLX-022 strain which has a good antagonistic effect on colletotrichum gloeosporioides and fusarium fusiforme, and the strain is preserved in China Center for Type Culture Collection (address: Wuhan University, Wuhan, China) and has a good antagonistic effect on colletotrichum gloeosporioides and fusarium fusiforme. The strain is preserved in China Center for Type Culture Collection (CCTCC) NO: M20252110 on September 24, 2025, the postal code is 430072, and the preservation number is CCTCC NO: M20252110. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The stenotrophomonas CLX-022 strain is high in antibacterial property and can be used for preparing a microbial preparation for treating / preventing strawberry anthracnose and rice bakanae disease.

The invention discloses application of a CsNOMT gene and sakuranetin in prevention and treatment of tea tree anthracnose. It is found for the first time that methyltransferase encoded by the tea tree-derived CsNOMT gene can efficiently catalyze naringenin to be converted into sakuranetin, the endogenous phytoalexin level of a tea tree is remarkably improved, and then the disease resistance of the tea tree-derived CsNOMT gene to Colletotrichum carolinae is enhanced. The silence of the CsNOMT gene can reduce the sakuranetin content by 30-40%, resulting in the increase of the scab area; and by overexpressing the gene, the sakuranetin content is increased by 30%, and the scab area is remarkably reduced. In addition, the growth of colletotrichum can be effectively inhibited by exogenously spraying 0.05-0.3 mM of sakuranetin, and the optimal concentration is 0.1-0.3 mM. The invention provides a new strategy for green prevention and control of tea tree anthracnose, and has the characteristics of high efficiency, safety and environmental friendliness.

The invention provides an endophyte Gliocladium roseum NG-12, which is named as Gliocladium roseum NG-12, and the preservation number of the endophyte Gliocladium roseum NG-12 is CGMCC (China General Microbiological Culture Collection Center) No. 41801. The endophyte Gliocladium roseum NG-12 provided by the invention has good root-knot nematode killing activity and antibacterial activity, has a good antagonistic effect on various plant pathogens such as fusarium oxysporum, colletotrichum gloeosporioides and colletotrichum gloeosporioides, is a potential biological preparation for preventing and treating root-knot nematode diseases, fusarium wilt, anthracnose and the like, and also has the effects of dissolving potassium, fixing nitrogen, dissolving phosphorus, preventing and treating the root-knot nematode diseases and the like. The capability of producing siderophore, protease and cellulase is realized. The gliocladium roseum provided by the invention has a wide development space and a good development and application prospect.

The invention discloses bacillus subtilis Bs1 as well as an antagonistic substance and application thereof. According to the invention, Bs1 with antagonism on colletotrichum gloeosporioides is separated and screened for the first time, an antagonistic substance is clear as Bs1 serine-producing protease Vpr, a Bs1 bacteriostasis mechanism and biological control potential excavation are analyzed in a multi-omics manner, a peripheral colonization rule of Bs1-Rif is clear, and an indoor control strategy of the strain Bs1 on cunninghamia lanceolata anthracnose is optimized. When the strain Bs1 is applied on the day when pathogenic bacteria are inoculated, a certain prevention and treatment effect is achieved, and it is indicated that the strain Bs1 can rapidly occupy the ecological niche; the average antibacterial rate of the strain Bs1 can reach 66.69%. The biocontrol strain and theoretical basis are provided for prevention and control of Chinese fir diseases, the application of biological prevention and control in forestry is promoted, the interaction mechanism of biocontrol bacteria and pathogenic bacteria can be deeply understood, and the sustainable development requirement of modern forestry is met.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

This invention discloses the CAPE1 peptide at the C-terminus of the PtPR1 protein and its applications. There are three types of CAPE1 peptides in the PtPR1 protein: PtPR1f (amino acid sequence shown in SEQ ID NO.1), PtPR1j (amino acid sequence shown in SEQ ID NO.2), and PtPR1l (amino acid sequence shown in SEQ ID NO.3). Experimental results show that the three CAPE1 peptides at the C-terminus of the PtPR1 protein provided by this invention can induce an immune defense response in woody plants at low concentrations. The use of these three CAPE1 peptides can also significantly improve the disease resistance of woody plants to *Colletotrichum gloeosporioides*, *Pseudomonas aeruginosa*, *Botrytis cinerea*, or *Phytophthora spp.*, without inhibiting the growth of these pathogens. Therefore, it can be applied to the prevention and control of woody plant diseases, providing a new approach to improving the disease resistance of woody plants and showing broad application prospects in the green control of woody plant diseases.

The application relates to the field of agricultural disease control technology, and particularly relates to the use of indole compounds and pharmaceutically acceptable salts, solvates, crystal forms, prodrugs, stereoisomers and tautomers thereof for preparing a medicine for inhibiting pathogenic key protein kinase CgSlt2 of pathogenic fungi. The application first proposes a new type of CgSlt2-targeted inhibitor, specifically, indole compounds ((S)-N-(1-(1H-indol-3-yl)-4-(methylamino)-4-oxobutan-2-yl)-8-bromo-1,6-naphthyridine-2-carboxamide) and ((S)-N-(2-((1H-indol-3-yl)-1-(methylamino)-1-oxopropyl)-8-bromo-1,6-naphthyridine-2-carboxamide) are used as CgSlt2 inhibitors, have the characteristics of high target specificity and high activity, and exhibit excellent pathogenicity inhibition of a strain of the complex group of Colletotrichum gloeosporioides.

The present invention provides a Bacillus tequilensis ZW-35 and its application, which relates to the technical field of microbial control. The Bacillus tequilensis ZW-35 is preserved in the China General Microbiological Culture Collection Center, and its taxonomic name is Bacillus tequilensis Bacillus tequilensis , and the preservation number is CGMCC No. 32878. The volatile substances in the metabolic process of Bacillus tequilensis ZW-35 can effectively inhibit the growth of Colletotrichum; and the volatile substances have high diffusion ability in air and soil pores. In the prevention and control of fruit diseases, the volatile substances can avoid direct contact with the fruits and will not leave residues on the fruit surface, thus avoiding the potential risks brought by direct contact.

The invention discloses a hydrazide compound taking syringic acid as a lead as well as a preparation method and application of the hydrazide compound, and a biological activity test result shows that all the compounds have excellent and broad-spectrum antibacterial activity on tested plant pathogenic fungi. Wherein the inhibition effect of most target compounds on plant pathogenic fungi is superior to that of positive control boscalid, the compound 11 has the best antibacterial effect on rhizoctonia solani, fusarium graminearum, alternaria solani, colletotrichum frutescens and colletotrichum sorghum, and EC50 values of the compound 11 are respectively 0.258 [mu] g / mL, 0.679 [mu] g / mL, 0.424 [mu] g / mL, 0.384 [mu] g / mL and 0.975 [mu] g / mL; for colletotrichum gloeosporioides, the compound 12 has the best antibacterial effect, and the EC50 value of the compound 12 is 0.989 mu g / mL; and secondly, a compound 11 with an EC50 value of 1.248 [mu] g / mL. The hydrazide compound prepared by taking syringic acid as a lead has excellent, broad-spectrum and efficient plant pathogenic microorganism resisting activity, and can be used for preventing and treating plant diseases caused by plant pathogenic microorganisms.

The invention provides an application of a CsSCPL36 gene, an encoded protein or a derivative thereof in regulating resistance of plants to anthracnose, and belongs to the technical field of biology. The invention provides a CsSCPL36 gene, an encoding protein of the CsSCPL36 gene or an application of a gene derivative containing the CsSCPL36 gene in regulation and control of resistance of plants to anthracnose and / or cultivation of anthracnose-resistant plant varieties. The CsSCPL36 gene or the CsSCPL36 gene coding protein can be used for improving the resistance of plants to anthracnose through forward regulation and control. According to the embodiment of the invention, by screening the influence of overexpression of different genes on anthracnose resistance, a result shows that overexpression of the CsSCPL36 gene can effectively improve the anthracnose resistance of the citrus, and a new way is provided for improving the resistance of the citrus to colletotrichum gloeosporioides.

The invention discloses paenibacillus FHY1 and application thereof, and belongs to the field of microorganisms. A strain of paenibacillus FHY1 is screened from Xinjiang populus euphratica juice, and experimental verification shows that a liquid culture of the paenibacillus FHY1 strain provided by the invention has a remarkable inhibition effect on magnaporthe oryzae, the bacteriostasis rate can reach 84%, and the inhibition rate on magnaporthe oryzae spore germination is 92%. And the rice growth can be promoted under the conditions of 150mM NaCl and 250mM NaCl. Meanwhile, the strain also has a good inhibition effect on pyricularia grisea, fusarium oxysporum, fusarium graminearum, sclerotinia sclerotiorum and colletotrichum gloeosporioides. Therefore, the invention provides a new strain with a good control effect on pathogenic fungi, and meanwhile, the growth and development of rice are remarkably promoted in a salt stress environment.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention belongs to the technical field of pesticides, and relates to 11 bactericide compositions for mainly preventing and treating crop diseases caused by pyricularia oryzae and colletotrichum gloeosporioides. The compound YJY-22 provided by the invention is novel in structure and unique in action mode, and has no cross resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil and tebuconazole. As the action mechanism of the compound YJY-22 is different from that of the medicament, and the compound YJY-22 and the medicament have no cross resistance, the compound YJY-22 and the medicament are compounded, so that the sterilization range can be expanded, the sterilization effect can be enhanced, the drug resistance of phytopathogen can be delayed, the safety to crops is good, the prevention and control spectrum is expanded, and the requirements of pesticide reduction and synergism are met.

The invention belongs to the technical field of RNA biological prevention and treatment, and particularly relates to application of dsRNA of a targeted actin coding gene. The invention provides application of dsRNA of a targeted actin coding gene in prevention and treatment of crop anthracnose, the nucleotide sequence of the dsRNA is shown as SEQ ID No.2, the dsRNA is obtained by transcription of a target gene segment of a colletotrichum CaACTIN gene, and the nucleotide sequence of the target gene segment of the colletotrichum CaACTIN gene is shown as SEQ ID No.1. The dsRNA is loaded by a nano-material layered double hydroxide, so that the dsRNA can be applied to prevention and treatment of crop anthracnose, and the target gene segment of the colletotrichum CaACTIN gene is obtained through transcription of the target gene segment of the colletotrichum CaACTIN gene and the target gene segment of the colletotrichum CaACTIN gene. The pathogenicity of mango colletotrichum gloeosporioides and banana colletotrichum gloeosporioides can be obviously reduced, and the application prospect of efficient and targeted control of crop anthracnose is achieved.

The invention provides a preparation method and application of bamboo fungus sourced sulfonic group modified carbon quantum dots, and belongs to the technical field of preparation of nano luminescent materials. The carbon quantum dot is prepared by taking bamboo fungus pileus as a carbon source and sodium sulfamate as a modifier through a hydrothermal method. The carbon quantum dot provided by the invention has a very strong inhibition effect on mango colletotrichum gloeosporioides. According to the invention, biological resources are effectively utilized, a foundation is laid for prevention and treatment of phytopathogen, and the application prospect is wide.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer combination, a kit and application of the LAMP detection primer combination and the kit. Six specific LAMP primer combinations are designed by utilizing a section of specific gene sequence of camellia anthracnose, and an LAMP rapid detection method of camellia anthracnose is established on the basis and is applied to field detection. The method has the advantages of rapidness, high efficiency, high accuracy, high sensitivity, capability of observing detection results by naked eyes and the like.

The invention discloses application of a sorghum SbPGK1 gene in improving plant disease resistance, and belongs to the technical field of biology. The nucleotide sequence of the sorghum SbPGK1 gene is as shown in SEQ ID NO.9. By analyzing phenotypes and related physiological indexes of SbPGK1 rice transgenic lines before and after inoculation of different pathogenic bacteria for infection and sorghum, the results show that compared with non-SbPGK1 gene rice lines, the SbPGK1 transgenic lines have obviously better resistance to magnaporthe oryzae and bacterial blight germs than wild types, silence SbPGK1 in sorghum has better resistance to rice blast germs and bacterial blight germs, and the SbPGK1 gene has better resistance to rice blast germs and bacterial blight germs. The SbPGK1 gene has the advantages that the SbPGK1 silent strain has reduced disease resistance to colletotrichum gloeosporioides, the SbPGK1 is overexpressed in sorghum, and the SbPGK1 overexpressed strain has enhanced disease resistance to colletotrichum gloeosporioides, so that the SbPGK1 gene is a potential disease-resistant breeding gene and can be used for plant disease-resistant genetic improvement.

The application discloses a strain of Burkholderia gladioli CT-5 and application thereof, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20242732, and the preservation date is December 5, 2024. The Burkholderia gladioli CT-5 provided by the application has a significant prevention and treatment effect (P<0.05) on root rot of Idesia polycarpa caused by Fusarium solani, and has a significant inhibitory effect on other various plant pathogenic fungi such as Schizophyllum commune, Diaporthe eres and Colletotrichum acutatum. In a pot experiment, the biomass of Idesia polycarpa seedlings, soil nutrients, SOD, POD and CAT enzyme activities of the CT-5 treatment group are significantly improved (P<0.05) compared with the pathogenic fungus control group (FP), indicating that the strain can promote plant growth, activate the activity of defense enzymes in the body, and thus effectively control the root rot of Idesia polycarpa, which provides strong theoretical basis and technical support for the sustainable development of Idesia polycarpa.

The invention provides a strain of Chryseobacterium ZJU645, a biological preparation, a culture method, a fermentation method and application, and particularly belongs to the technical field of microbial control. The Chryseobacterium sp. ZJU645 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.35186, and the preservation date is July 10, 2025. The Chryseobacterium ZJU645 disclosed by the invention has a broad-spectrum antifungal effect, and has a relatively high antibacterial effect on alternaria alternata, sclerotinia sclerotiorum, fusarium oxysporum, paniculate corn, colletotrichum gloeosporioides and botrytis cinerea, and a fermentation product of the Chryseobacterium ZJU645 is stable. Through further optimization of fermentation conditions of the Chryseobacterium sp. ZJU645, the disease resistance of the Chryseobacterium sp. ZJU645 is improved, and a new biological control means is provided for more efficient prevention and treatment of agricultural diseases, especially mulberry diseases.

The invention belongs to the technical field of microorganisms, and particularly discloses a bacillus aryabhattai strain and application thereof, the bacillus aryabhattai strain is identified as the bacillus aryabhattai strain through morphology, physiology, biochemistry and 16S rDNA (ribosomal deoxyribonucleic acid) molecules, is named as GR-6, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.37494. The bacillus aryabhattai GR-6 disclosed by the invention has an in-vitro control effect of 67.58% and a living potting control effect of 38.67% on tomato gray leaf spot disease, and has good nitrogen fixation and potassium solubilization capabilities, and generated volatile substances have an obvious inhibition effect on various plant pathogenic fungi such as Stemphylium solani, Corynespora cassiicola, Botryis cinerea, fusarium oxysporum and Colletotrichum capsici; according to the present invention, the genome contains Surfactin, Macrolactin, Difficidin and other lipopeptide and polyketone antibiotic synthesis related genes, such that the biocontrol effect is significant, the growth promoting function and the broad-spectrum antibacterial ability are provided, the environmental protection is provided, the new microbial resource is provided for the green prevention and control of the tomato gray leaf spot disease, and the important application development value is provided.

The invention relates to the technical field of microbial genetic transformation, and particularly discloses a PEG (Polyethylene Glycol)-mediated camellia anthracnose protoplast genetic transformation method which comprises the following steps: obtaining young and tender camellia anthracnose mycelia, filtering and collecting protoplast by using double-layer lens wiping paper, obtaining protoplast precipitate, purifying the protoplast by using an STC solution, and resuspending the protoplast by using the STC to obtain the PEG-mediated camellia anthracnose protoplast. The preparation method comprises the following steps: adding plasmids into a camellia anthracnose protoplast, lightly and uniformly mixing, standing at room temperature, adding a PTC solution, uniformly mixing, standing at room temperature, adding a PTC solution, uniformly mixing, standing at room temperature, pouring into a TB3 solid culture medium, uniformly mixing, pouring into a plate, culturing overnight, covering with a potato dextrose agar culture medium containing geneticin, and culturing at room temperature. The transformant is inoculated to a potato dextrose agar culture medium containing heredity mycin, the transformant with stable characters is screened, and the strain obtained through genetic transformation is obtained.By means of the method, construction of genetic transformation of the camellia anthracnose bacteria is achieved, the time needed by genetic transformation is shortened, and the transformant expressing red fluorescence and stably inheriting is obtained.

The invention discloses application of mortierella 2K4 as a biocontrol bacterium for poplar anthracnose, and belongs to the technical field of microbial pesticides. A plate confrontation experiment proves that the strain has a remarkable antagonistic effect on poplar anthracnose pathogenic bacteria colletotrichum gloeosporioides, and the bacteriostasis rate reaches 50.9%. The fermentation filtrate also shows a concentration-dependent antibacterial effect, and the antibacterial rate of 50% concentration is 45.50%. In-vitro leaf and pot experiments further prove that the scab diameter and disease index of the poplar anthracnose can be effectively reduced by applying the strain, and the prevention and treatment effect exceeds 59.45%. The invention provides an efficient and environment-friendly biocontrol bacterium resource for green prevention and control of poplar anthracnose and an application method of the biocontrol bacterium resource.