Glutamine synthetase gene and application
A glutamine and synthetase technology, applied in the direction of enzymes, ligases, and the introduction of foreign genetic material using vectors, can solve the problems of difficulty in screening high-expressing cell lines, and the expression efficiency cannot meet production requirements, and achieve the effect of improving expression.
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[0028] 1. Construction of recombinant expression vector
[0029] The light chain nucleotide sequence of antibody mAb01 is shown in SEQ ID NO:1, and the heavy chain nucleotide sequence is shown in SEQ ID NO:2. The light chain nucleotide sequence of the antibody mAb02 is shown in SEQ ID NO:3, and the heavy chain nucleotide sequence is shown in SEQ ID NO:4. The light chain nucleotide sequence of antibody mAb03 is shown in SEQ ID NO:5, and the heavy chain nucleotide sequence is shown in SEQ ID NO:6.
[0030] The eukaryotic expression vector containing the GS16034 gene and the target antibody sequence can be constructed by the following steps: (1) replace the sequence between the 2136-2931bp of the initial vector pcDNA3.1 (+) site with mGS to obtain the intermediate vector pcDNA3.1-mGS, and then The initial vector pcDNA3.1 (+) site 232-819bp sequence was cloned into the intermediate vector pcDNA3.1-mGS between the 1252-1253bp site to obtain a new vector pcDNA3.1-mGS-DGV, and the v...
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